ID pCAGGS-FLAG-hUb-K33 standard; circular DNA; ; 5000 BP. DE LMBP 5899. CC Accession number: LMBP 5899 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 4-JUN-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT intron 4025..4949 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid intron" FT promoter 3656..4997 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT promoter 3273..3650 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT CDS complement(2273..3133) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(1331..1732) FT /vntifkey="33" FT /label=pMB1\ori FT terminator complement(1142..1216) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 1142..1216 FT /vntifkey="43" FT /label=SV40\polyA\early FT rep_origin 1036..1111 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter complement(812..894) FT /vntifkey="30" FT /label=lac\promoter FT misc_feature 378..734 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT terminator 286..377 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT CDS 46..276 FT /vntifkey="4" FT /label=hUBB\mutated\mature\sequence FT mutation 61..63 FT /vntifkey="62" FT /label=K6R FT /note="wt: AAG" FT mutation 76..78 FT /vntifkey="62" FT /label=K11R FT /note="wt: AAG" FT mutation 124..126 FT /vntifkey="62" FT /label=K27R FT /note="wt: AAG" FT mutation 130..132 FT /vntifkey="62" FT /label=K29R FT /note="wt: AAG" FT mutation 187..189 FT /vntifkey="62" FT /label=K48R FT /note="wt: AAG" FT mutation 232..234 FT /vntifkey="62" FT /label=K63R FT /note="wt: AAA" SQ Sequence 5000 BP; 1058 A; 1327 C; 1398 G; 1216 t; 1 other; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgcgatgca gatcttcgtg 60 aggacccwga ccggcaggac catcactctg gaggtggagc ccagtgacac catcgaaaat 120 gtgagggcca gaatccaaga taaggaaggc atcccccccg accagcagag gctcatcttt 180 gcaggcaggc agctggaaga tggccgcact ctttctgact acaacatcca gagggagtcg 240 accctgcacc tggtcctgcg tctcagaggt gggtaggtcg acgaagatct ttttccctct 300 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg 360 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca 420 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca 480 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg 540 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt 600 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat 660 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt 720 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt 780 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 840 gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 900 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa 960 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 1020 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 1080 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 1140 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 1200 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 1260 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg 1320 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 1380 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcagggata 1440 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 1500 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 1560 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 1620 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 1680 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 1740 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 1800 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 1860 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 1920 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 1980 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 2040 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 2100 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 2160 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 2220 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 2280 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 2340 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 2400 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 2460 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 2520 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 2580 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 2640 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 2700 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 2760 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 2820 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 2880 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 2940 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 3000 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 3060 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 3120 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 3180 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 3240 catttccccg aaaagtgcca cctgggtcga tcgacattga ttattgacta gttattaata 3300 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 3360 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 3420 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 3480 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 3540 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 3600 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt 3660 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta 3720 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc 3780 cccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc 3840 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg 3900 gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt 3960 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc 4020 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg 4080 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt 4140 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg 4200 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct 4260 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg 4320 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg 4380 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc 4440 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg 4500 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg 4560 ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc 4620 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg 4680 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt 4740 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc 4800 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac 4860 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc 4920 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt 4980 ctcatcattt tggcaaagaa 5000 //