LOCUS       pET3E-His-AU1-hUb-K11        4924 bp    DNA     circular     23-MAY-2011
DEFINITION  LMBP 5907.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5907
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-MAY-2011
            
            
            
COMMENT     VNTDATE|590940948|
COMMENT     VNTNAME|pET3E-His-AU1-hUb-K11|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pET3E-His-AU1-hUb-K11" 0 4924 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 2 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "2mu ori" 
COMMENT             "AGAAAGTATAGGAACTTCAGAGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGCACTTTCAAAAAACCAAAAACGCACCGGACTGTAACGAGCTACTAAAATATTGCGAATACCGCTTCCACAAACATTGCTCAAAAGTATCTCTTTGCTATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACTCGACCTCTACATTTTTTATGTTTATCTCTAGTATTACTCTTTAGACAAAAAAATTGTAGTAAGAACTATTCATAGAGTGAATCGAAAACAATACGAAAATGTAAACATTTCCTATACGTAGTATATAGAGACAAAATAGAAGAAACCGTTCATAATTTTCTGACCAATGAAGAATCATCAACGCTATCACTTTCTGTTCACAAAGTATGCGCAATCCACATCGGTATAGAATATAATCGGGGATGCCTTTATCTTGAAAAAATGCACCCGCAGCTTCGCTAGTAATCAGTAAACGCGGGAAGTGGAGTCAGGCTTTTTTTATGGAAGAGAAAATAGACACCAAAGTAGCCTTCTTCTAACCTTAACGGACCTACAGTGCAAAAAGTTATCAAGAGACTGCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTTAGAAAAATAGCGCTCTCGGGATGCATTTTTGTAGAACAAAAAAGAAGTATAGATTCTTTGTTGGTAAAATAGCGCTCTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTTTACAAAAATGAAGCACAGATTCTTCGTTGGTAAAATAGCGCTTTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTCTACAAAATGAAGCACAGATGCTTCGTT" 
COMMENT             "Fragment of pAS3; Length: 981" 0 (CStringList) 0) 
COMMENT         #1=(COligo "3' FR of \337-globin" 
COMMENT             "AATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATATGGGAGGGCAAATCATTTAAAACATCAGAATGAGTATTTGGTTTAGAGTTTGGCAACATATGCCCATATGCTGGCTGCCATGAACAAAGGTTGGCTATAAAGAGGTCATCAGTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTGACTTGAGGTTAGATTTTTTTTATATTTTGTTTTGTGTTATTTTTTTCTTTAACATCCCTAAAATTTTCCTTACATGTTTTACTAGCCAGATTTTTCCTCCTCTCCTGACTACTCCCAGTCATAGCTGTCCCTCTTCTCTTATGGAGATC" 
COMMENT             "Fragment of pCAGGS-FLAGAVPShOMI; Length: 357" 0 (CStringList) 0) 
COMMENT         #2=(COligo "3' MMLV-LTR" 
COMMENT             "AATGAAAGACCCCACCTGTAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTCAGGAACAGATGGAACAGCTGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGAACAGCTGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCAGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGGGCGCCAGTCCTCCGATTGACTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTTGCAGTTGCATCCGACTTGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCGTCAGCGGGGGTCTTTCATT" 
COMMENT             "Fragment of LNXCO3; Length: 594" 0 (CStringList) 0) 
COMMENT         #3=(COligo "3' MSCV-PCMV LTR" 
COMMENT             "CTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTTAGGAACAGAGAGACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCCGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCCTCCGATAGACTGCGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTTGCAGTTGCATCCGACTTGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCGTCAGCGGGGGTCTTTCA" 
COMMENT             "Fragment of pMSCV-F-BirA; Length: 485" 0 (CStringList) 0) 
COMMENT         #4=(COligo "5' MMLV-LTR" 
COMMENT             "TTTGAAAGACCCCACCCGTAGGTGGCAAGCTAGCTTAAGTAACGCCACTTTGCAAGGCATGGAAAAATACATAACTGAGAATAGAAAAGTTCAGATCAAGGTCAGGAACAAAGAAACAGCTGAATACCAAACAGGATATCTGTGGTAAGCGGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGAGACAGCTGAGTGATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCGGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCAGCCCTCAGCAGTTTCTAGTGAATCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAAATGACCCTGTACCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCCGCTCTCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCTTCCGATAGACTGCGTCGCCCGGGTACCCGTATTCCCAATAAAGCCTCTTGCTGTTTGCATCCGAATCGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCACGACGGGGGTCTTTCATT" 
COMMENT             "Fragment of LNXCO3; Length: 589" 0 (CStringList) 0) 
COMMENT         #5=(COligo "5' MSCV-PCMV LTR" 
COMMENT             "TGAAAGACCCCACCTGTAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTTAGGAACAGAGAGACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCCGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCCTCCGATAGACTGCGTCGCCCGGGTAC" 
COMMENT             "Fragment of pMSCV-F-BirA; Length: 407" 0 (CStringList) 0) 
COMMENT         #6=(COligo "5' UTR of AOX1" 
COMMENT             "ACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAA" 
COMMENT             "Fragment of pPP1MFmds1; Length: 165" 0 (CStringList) 0) 
COMMENT         #7=(COligo "ADE2 (1)" 
COMMENT             "ATGGATTCTAGAACAGTTGGTATATTAGGAGGGGGACAATTGGGACGTATGATTGTTGAGGCAGCAAACAGGCTCAACATTAAGACGGTAATACTAGATGCTGAAAATTCTCCTGCCAAACAAATAAGCAACTCCAATGACCACGTTAATGGCTCCTTTTCCAATCCTCTTGATATCGAAAAACTAGCTGAAAAATGTGATGTGCTAACGATTGAGATTGAGCATGTTGATGTTCCTACACTAAAGAATCTTCAAGTAAAACATCCCAAATTAAAAATTTACCCTTCTCCAGAAACAATCAGATTGATACAAGACAAATATATTCAAAAAGAGCATTTAATCAAAAATGGTATAGCAGTTACCCAAAGTGTTCCTGTGGAACAAGCCAGTGAGACGTCCCTATTGAATGTTGGAAGAGATTTGGGTTTTCCATTCGTCTTGAAGTCGAGGACTTTGGCATACGATGGAAGAGGTAACTTCGTTGTAAAGAATAAGGAAATGATTCCGGAAGCTTTGGAAGTACTGAAGGATCGTCCTTTGTACGCCGAAAAATGGGCACCATTTACTAAAGAATTAGCAGTCATGATTGTGAGATCTGTTAACGGTTTAGTGTTTTCTTACCCAATTGTAGAGACTATCCACAAGGACAATATTTGTGACTTATGTTATGCGCCTGCTAGAGTTCCGGACTCCGTTCAACTTAAGGCGAAGTTGTTGGCAGAAAATGCAATCAAATCTTTTCCCGGTTGTGGTATATTTGGTGTGGAAATGTTCTATTTAGAAACAGGGGAATTGCTTATTAACGAAATTGCCCCAAGGCCTCACAACTCTGGACATTATACCATTGATGCTTGCGTCACTTCTCAATTTGAAGCTCATTTGAGATCAATATTGGATTTGCCAATGCCAAAGAATTTCACATCTTTCTCCACCATTACAACGAACGCCATTATGCTAAATGTTCTTGGAGACAAACATACAAAAGATAAAGAGCTAGAAA" 
COMMENT             "Fragment of pAS3; Length: 1000" 0 (CStringList) 0) 
COMMENT         #8=(COligo "ADE2 (2)" 
COMMENT             "CTTGCGAAAGAGCATTGGCGACTCCAGGTTCCTCAGTGTACTTATATGGAAAAGAGTCTAGACCTAACAGAAAAGTAGGTCACATAAATATTATTGCCTCCAGTATGGCGGAATGTGAACAAAGGCTGAACTACATTACAGGTAGAACTGATATTCCAATCAAAATCTCTGTCGCTCAAAAGTTGGACTTGGAAGCAATGGTCAAACCATTGGTTGGAATCATCATGGGATCAGACTCTGACTTGCCGGTAATGTCTGCCGCATGTGCGGTTTTAAAAGATTTTGGCGTTCCATTTGAAGTGACAATAGTCTCTGCTCATAGAACTCCACATAGGATGTCAGCATATGCTATTTCCGCAAGCAAGCGTGGAATTAAAACAATTATCGCTGGAGCTGGTGGGGCTGCTCACTTGCCAGGTATGGTGGCTGCAATGACACCACTTCCTGTCATCGGTGTGCCCGTAAAAGGTTCTTGTCTAGATGGAGTAGATTCTTTACATTCAATTGTGCAAATGCCTAGAGGTGTTCCAGTAGCTACCGTCGCTATTAATAATAGTACGAACGCTGCGCTGTTGGCTGTCAGACTGCTTGGCGCTTATGATTCAAGTTATACAACGAAAATGGAACAGTTTTTATTAAAGCAAGAAGAAGAAGTTCTTGTCAAAGCACAAAAGTTAGAAACTGTCGGTTACGAAGCTTATCTAGAAAACAAGTAA" 
COMMENT             "Fragment of pAS3; Length: 716" 0 (CStringList) 0) 
COMMENT         #9=(COligo "ADH1 promoter" 
COMMENT             "TGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAGTTTGCCGCTTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACTCCAAG" 
COMMENT             "Fragment of pAS3; Length: 404" 0 (CStringList) 0) 
COMMENT         #10=(COligo "ADH1 terminator" 
COMMENT              "GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTC" 
COMMENT              "Fragment of pRH007; Length: 102" 0 (CStringList) 0) 
COMMENT         #11=(COligo "AG promoter (1)" 
COMMENT              "GAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCCCCCCCCAGGCGGGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAAAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGTTGCGCGCTGCCTTCCCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAGGGCTCGGGGGAAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCAG" 
COMMENT              "Fragment of pCAGGS-FLAGAVPShOMI; Length: 1000" 0 (CStringList) 0) 
COMMENT         #12=(COligo "AG promoter (2)" 
COMMENT              "GGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGAAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAAGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAA" 
COMMENT              "Fragment of pCAGGS-FLAGAVPShOMI; Length: 342" 0 (CStringList) 0) 
COMMENT         #13=(COligo "amp promoter" 
COMMENT              "CAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCT" 
COMMENT              "Fragment of pdEYFP; Length: 96" 0 (CStringList) 0) 
COMMENT         #14=(COligo "AMP RBS" "GATAAATGCTTCAATAATATTGAAAAAGGAAGAGT" 
COMMENT              "Fragment of pdECFP; Length: 35" 0 (CStringList) 0) 
COMMENT         #15=(COligo "ampicillin" 
COMMENT              "ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA" 
COMMENT              "Fragment of pEF6-E-mTRAF2; Length: 861" 0 (CStringList) 0) 
COMMENT         #16=(COligo "AOX1 promoter" 
COMMENT              "AACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGCCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTG" 
COMMENT              "Fragment of pPP1MFmds1; Length: 766" 0 (CStringList) 0) 
COMMENT         #17=(COligo "AOX1 terminator" 
COMMENT              "TCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGAAGTTCGTTTGTGC" 
COMMENT              "Fragment of pPP1MFmds1; Length: 260" 0 (CStringList) 0) 
COMMENT         #18=(COligo "attR1" 
COMMENT              "CATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTTTTGTACAAACTTGT" 
COMMENT              "Fragment of pRH007; Length: 125" 0 (CStringList) 0) 
COMMENT         #19=(COligo "attR2" 
COMMENT              "CATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGT" 
COMMENT              "Fragment of pRH007; Length: 125" 0 (CStringList) 0) 
COMMENT         #20=(COligo "birA" 
COMMENT              "ATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTACCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGGCGCAAACTTATATTTGTCGATGTTCTGGCGTCTGGAACAAGGCCCGGCGGCGGCGATTGGTTTAAGTCTGGTTATCGGTATCGTGATGGCGGAAGTATTACGCAAGCTGGGTGCAGATAAAGTTCGTGTTAAATGGCCTAATGACCTCTATCTGCAGGATCGCAAGCTGGCAGGCATTCTGGTGGAGCTGACTGGCAAAACTGGCGATGCGGCGCAAATAGTCATTGGAGCCGGGATCAACATGGCAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAA" 
COMMENT              "Fragment of MSCVp-F-BirA; Length: 966" 0 (CStringList) 0) 
COMMENT         #21=(COligo "BK ori" "GAGGCCGAGGCCGCCTCTGCCTC" 
COMMENT              "Fragment of pBK-1; Length: 23 " 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #22=(COligo "blasticidin" 
COMMENT              "ATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAA" 
COMMENT              "Fragment of pEF6-E-mTRAF2; Length: 399" 0 (CStringList) 0) 
COMMENT         #23=(COligo "bleomycin" 
COMMENT              "ATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACGGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCAAGCTGGAGTTCTTCGCCCACCCCGGGCTCGATCCCCTCGCGAGTTGGTTCAGCTGCTGCCTGAGGCTGGACGACCTCGCGGAGTTCTACCGGCAGTGCAAATCCGTCGGCATCCAGGAAACCAGCAGCGGCTATCCGCGCATCCATGCCCCCGAACTGCAGGAGTGGGGAGGCACGATGGCCGCTTTGGTCGACCCGGACGGGACGCTCCTGCGCCTGATACAGAACGAATTGCTTGCAGGCATCTCATGA" 
COMMENT              "Fragment of pSCUDMFE6L2; Length: 381" 0 (CStringList) 0) 
COMMENT         #24=(COligo "bovine growth hormone polyA" 
COMMENT              "CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG" 
COMMENT              "Fragment of pEF1hCASP-9S-His; Length: 225" 0 (CStringList) 0) 
COMMENT         #25=(COligo "CaGAL1 promoter (1)" 
COMMENT              "AGATCTGATATTGACGAAGAGGAACCAATGTAACGTTACACTGAAGAAAACACACAATAAACGGGAAGAAACGGTGTAAAAGTGTGAAAATAATTTTTGAATATCATTTCCCTTGGTTTAATTCCAAACGAAACGTGTTTTTTTTAGAGAATGGGAATTCTTATTGGATGTCTAGATTGTTTGTTTACTCCAGACTGTGCACAAAAACGTTTGGATGGATGATCAGAAGATATTTTTAGGCTTAGCTCTAAATATAAGAAATGATGCTTGAAAAACCAGACAGAAATTGAGTTTCAAAAATTGGTAATGTGAGGTATTAGTCAACTAACCAAATAACAATGCAAACCGGTTGATACATTTCATTTTGAAAATAATGAAACTGGAATTGGATGACCAGCACACAAACACATAAAGTAATTATGGGAATTAGAAGCGAACATAGAGGAGTACTTGGCCACGAACAGAATACAAGTGGGAACACTATTTTCTCCATTGTTTTAGTTCTGTTTTTTTGTCAGCCTAGTTTTGTGCTATGTGTAAAAAATATTGCCAAGAAAAAAAGCTTGTTTTGTGGCCAGTGTCCGAAAAAAATTTTGGGGAATCTTCGGATTAATTTATGTTTTCATTCCATCGGGGAAAGTGGGGGGGAAAAAATTTTAAGCAGTTCACAAAACCTTCCAAAAAATATATGGACAAAGATGATTGTATTTTCCCGACACCAAAATCATAATTAATTATGAGAAAGTTAAATGTAACGTTACAATTTATGTTTATTTGAAGGTGAAAAGCGATTTATGATTTTTCCGAAATGAAAATTTTTTTTAGGTTTATTTTTTTTGTCGGGCAAAGAAAAACTGAACAAGGATTATTAAAATTTTTGGTGTTTGTTTGTGTCTGGAGAATTCATTCCTCTCTCATCTTCACACAATGTTTAGACATCTGACACGATTCATGATAGTTCGGTTTCCGGGGTTGGTGTTTAGTTTTCGTTTTTCTTTTT" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 1000" 0 (CStringList) 0) 
COMMENT         #26=(COligo "CaGAL1 promoter (2)" 
COMMENT              "TTTTGGAAAGAATGTTTTAGCTCATTGGTTTTCTTTCTTCATTCAATAGTTTTGAAAGAATTTGCCCACTTGTTATTACAATCATATAAAATTAAACTTTGATATAAAATAGAGTTTGAAAGTTTCCCAGATCCTTTTTGATTTCTTTGTAAATTTTTTTTTCTCCCACATATACACACATACAAACCGATTTTTATAAGAAAGAGTTATACC" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 213" 0 (CStringList) 0) 
COMMENT         #27=(COligo "calmodulin-binding peptide" 
COMMENT              "AAGCGACGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGCCAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTT" 
COMMENT              "Fragment of pCal-c-A7M8K; Length: 78" 0 (CStringList) 0) 
COMMENT         #28=(COligo "CaMV promoter" 
COMMENT              "GATCTCCTTTGCCCCGGAGATCACCATGGACGACTTTCTCTATCTCTACGATCTAGGAAGAAAGTTCGACGGAGAAGGTGACGATACCATGTTCACCACCGATAATGAGAAGATTAGCCTCTTCAATTTCAGAAAGAATGCTGACCCACAGATGGTTAGAGAGGCCTACGCGGCAGGTCTCATCAAGACGATCTACCCGAGTAATAATCTCCAGGAGATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCATAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAGAAAGTAGTTCCTACTGAATCAAAGGCCATGGAGTCAAAAATTCAGATCGAGGATCTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCT" 
COMMENT              "Fragment of pEX1SOD; Length: 947" 0 (CStringList) 0) 
COMMENT         #29=(COligo "CaURA3" 
COMMENT              "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #30=(COligo "ccdB" 
COMMENT              "ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA" 
COMMENT              "Fragment of pRH001; Length: 306" 0 (CStringList) 0) 
COMMENT         #31=(COligo "CEN6/ARSH4 ori" 
COMMENT              "CTTTTCATCACGTGCTATAAAAATAATTATAATTTAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAAAGAAATTAAAGAAAAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGATCGCCAACAAATACTACCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTTTCATCTTGTCTGTGTAGAAGACCACACACGAAAATCCTGTGATTTTACATTTTACTTATCGTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAGTACGCTTTTTGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTAACTCTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACACAGAGTAAATTCCCAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTACAGGCAAGCGATCCGTCCTAAGAAACCA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 525" 0 (CStringList) 0) 
COMMENT         #32=(COligo "chloramphenicol" 
COMMENT              "ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAA" 
COMMENT              "Fragment of pRH001; Length: 660" 0 (CStringList) 0) 
COMMENT         #33=(COligo "ColE1 ori" 
COMMENT              "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCTATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCTGCTACGTGGCCTTCTTCCTGTTCCTGGTCTTTTGCTCACATGTTCTTTCCGGCCTTATCCCCTGATTCTGTGGATAACTGTGTTACCGTTTTTGTGTGAGTCAGTACCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAAAAGC" 
COMMENT              "Fragment of pPLa2311trpA1; Length: 392" 0 (CStringList) 0) 
COMMENT         #34=(COligo "CYC1 promoter" 
COMMENT              "GACAATCTTACATGGTCTACCTTTGATGACAACGAAACCATTCTTTCTCAAGGCAGAACATTGCATTGGGTAGGTGGCGGAGGCACCAGCGTCAGCATTTTCAAAGGTTGTGTTCTTCGTCAGACATGTTTTAGTGTGTGAATGAAATAGGTGTATGTTTTCTTTTGCAGACAATAATTAGGAACAAGGTAAGGGAACTAAAGTGTAGAATAAGAATTAAAAAAGAAGAACAAGTTGAAAGGCAAGTTGAAATTTCAAGAAAAAAGTCAATTGAAGTACAGTAAATTGACCTGAATATATCTGAGTTCCGAGCAACAATGAGTTTACCGAAGAGAACAATGGAATAGGAAACTTTGAACGAAGAAAGGAAAGCAGGAAAGGAAAAAATTTTTAGGCTCGAGAACAATAGGGCAAAAAAACAGGCAACGAACGAACAAATGGAAAAACGAAAAAAAAAAAAACACAGAAAAGAATGCAGAAAGTTGTAAACTGAAAAAAAAAAAAAAAAAAGGTGAACACAGGAAAAAAAATAAAAAAAAAAAAAAAGGAGGACGAAACAAAAAAGTGAAAAAAAATGAAAATTTTTTTGGAAAACCAAGAAATGAATTATATTTCCGTGTGAGACGACATCGTCGAATATGATTCAGGGTAACAGTATTTATGTAATCAATTTCCTACCTGAATCTAAAATTCCCGGGAGCAAGATCAAGATGTTTTCACCGATCTTTCCGGTCTCTTTGGCCGGGGTTTACGGACGATGACCGAAGACCAAGCGCCAGCTCATTTGGCGAGCGTTGGTTGGTGGATCAAGCCCACGCGTAGGCAATCCTCGAGCAGATCCGCCAGGCGTGTATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCT" 
COMMENT              "Fragment of pEX2; Length: 982" 0 (CStringList) 0) 
COMMENT         #35=(COligo "CYC1 terminator" 
COMMENT              "ACAGGCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAG" 
COMMENT              "Fragment of pAS2; Length: 276  (laatste N: G (276) ipv A (275) -> midden HindIII)" 
COMMENT              0 (CStringList) 0) 
COMMENT         #36=(COligo "CYH2" 
COMMENT              "ATGCCTTCCAGATTCACTAAGACTAGAAAGCACAGAGGTCACGTCTCAGCCGGTAAAGGTCGTATCGGTAAGCACAGAAAGCACCCCGGTGGTAGAGGTATGGCCGGTGGTCAACATCACCACAGAATTAACATGGATAAATACCATCCAGGTTATTTCGGTAAGGTTGGTATGAGATACTTCCACAAGCAACAAGCTCATTTCTGGAAGCCAGTCTTGAACTTGGACAAATTGTGGACATTGATCCCAGAAGACAAGAGAGACCAATACTTGAAATCTGCTTCTAAGGAAACTGCTCCAGTTATTGACACTTTGGCAGCCGGTTACGGTAAGATCTTGGGTAAGGGTAGAATTCCAAATGTTCCAGTTATCGTCAAAGCTAGATTCGTCTCCAAGTTGGCTGAAGAAAAAATCAGAGCTGCTGGTGGTGTTGTTGAATTGATCGCTTAA" 
COMMENT              "Fragment of pAS2; Length: 450" 0 (CStringList) 0) 
COMMENT         #37=(COligo "E-tag" "GGNGCNCCNGTNCCNTAYCCNGAYCCNYTNGARCCNCGN" 
COMMENT              " Fragment of pYD1TSE ; Length: 39" 0 (CStringList) 0) 
COMMENT         #38=(COligo "EBNA1 (1)" 
COMMENT              "ATGTCTGACGAGGGGCCAGGTACAGGACCTGGAAATGGCCTAGGAGAGAAGGGAGACACATCTGGACCAGAAGGCTCCGGCGGCAGTGGACCTCAAAGAAGAGGGGGTGATAACCATGGACGAGGACGGGGAAGAGGACGAGGACGAGGAGGCGGAAGACCAGGAGCCCCGGGCGGCTCAGGATCAGGGCCAAGACATAGAGATGGTGTCCGGAGACCCCAAAAACGTCCAAGTTGCATTGGCTGCAAAGGGACCCACGGTGGAACAGGAGCAGGAGCAGGAGCGGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGTGGAGGCCGGGGTCGAGGAGGCA" 
COMMENT              "Fragment of pREP4FLAG; Length: 1000" 0 (CStringList) 0) 
COMMENT         #39=(COligo "EBNA1 (2)" 
COMMENT              "GTGGAGGCCGGGGTCGAGGAGGTAGTGGAGGCCGGGGTCGAGGAGGTAGTGGAGGCCGCCGGGGTAGAGGACGTGAAAGAGCCAGGGGGGGAAGTCGTGAAAGAGCCAGGGGGAGAGGTCGTGGACGTGGAGAAAAGAGGCCCAGGAGTCCCAGTAGTCAGTCATCATCATCCGGGTCTCCACCGCGCAGGCCCCCTCCAGGTAGAAGGCCATTTTTCCACCCTGTAGGGGAAGCCGATTATTTTGAATACCACCAAGAAGGTGGCCCAGATGGTGAGCCTGACGTGCCCCCGGGAGCGATAGAGCAGGGCCCCGCAGATGACCCAGGAGAAGGCCCAAGCACTGGACCCCGGGGTCAGGGTGATGGAGGCAGGCGCAAAAAAGGAGGGTGGTTTGGAAAGCATCGTGGTCAAGGAGGTTCCAACCCGAAATTTGAGAACATTGCAGAAGGTTTAAGAGCTCTCCTGGCTAGGAGTCACGTAGAAAGGACTACCGACGAAGGAACTTGGGTCGCCGGTGTGTTCGTATATGGAGGTAGTAAGACCTCCCTTTACAACCTAAGGCGAGGAACTGCCCTTGCTATTCCACAATGTCGTCTTACACCATTGAGTCGTCTCCCCTTTGGAATGGCCCCTGGACCCGGCCCACAACCTGGCCCGCTAAGGGAGTCCATTGTCTGTTATTTCATGGTCTTTTTACAAACTCATATATTTGCTGAGGTTTTGAAGGATGCGATTAAGGACCTTGTTATGACAAAGCCCGCTCCTACCTGCAATATCAGGGTGACTGTGTGCAGCTTTGACGATGGAGTAGATTTGCCTCCCTGGTTTCCACCTATGGTGGAAGGGGCTGCCGCGGAGGGTGATGACGGAGATGACGGAGATGAAGGAGGTGATGGAGATGAGGGTGAGGAAGGGCAGGAGTGA" 
COMMENT              "Fragment of pREP4FLAG; Length: 926" 0 (CStringList) 0) 
COMMENT         #40=(COligo "ECFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTGGGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACATCAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pECFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #41=(COligo "EGFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pEGFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #42=(COligo "EK recognition site" "GAYGAYGAYGAYAAR" "Length: 15 bp" 0 
COMMENT              (CStringList) 0) 
COMMENT         #43=(COligo "Epstein-Barr oriP (1)" 
COMMENT              "CAGGAAAAGGACAAGCAGCGAAAATTCACGCCCCCTTGGGAGGTGGCGGCATATGCAAAGGATAGCACTCCCACTCTACTACTGGGTATCATATGCTGACTGTATATGCATGAGGATAGCATATGCTACCCGGATACAGATTAGGATAGCATATACTACCCAGATATAGATTAGGATAGCATATGCTACCCAGATATAGATTAGGATAGCCTATGCTACCCAGATATAAATTAGGATAGCATATACTACCCAGATATAGATTAGGATAGCATATGCTACCCAGATATAGATTAGGATAGCCTATGCTACCCAGATATAGATTAGGATAGCATATGCTACCCAGATATAGATTAGGATAGCATATGCTATCCAGATATTTGGGTAGTATATGCTACCCAGATATAAATTAGGATAGCATATACTACCCTAATCTCTATTAGGATAGCATATGCTACCCGGATACAGATTAGGATAGCATATACTACCCAGATATAGATTAGGATAGCATATGCTACCCAGATATAGATTAGGATAGCCTATGCTACCCAGATATAAATTAGGATAGCATATACTACCCAGATATAGATTAGGATAGCATATGCTACCCAGATATAGATTAGGATAGCCTATGCTACCCAGATATAGATTAGGATAGCATATGCTATCCAGATATTTGGGTAGTATATGCTACCCATGGCAACATTAGCCCACCGTGCTCTCAGCGACCTCGTGAATATGAGGACCAACAACCCTGTGCTTGGCGCTCAGGCGCAAGTGTGTGTAATTTGTCCTCCAGATCGCAGCAATCGCGCCCCTATCTTGGCCCGCCCACCTACTTATGCAGGTATTCCCCGGGGTGCCATTAGTGGTTTTGTGGGCAAGTGGTTTGACCGCAGTGGTTAGCGGGGTTACAATCAGCCAAGTTATTACACCCTTATTTTACAGTCCAAAACCGCAGGGCGGCGTGTGGGGGCTGACGCGTGCCCCCACTCCACAATTT" 
COMMENT              "Fragment of pREP4FLAG; Length: 1000" 0 (CStringList) 0) 
COMMENT         #44=(COligo "Epstein-Barr oriP (2)" 
COMMENT              "CAAAAAAAAGAGTGGCCACTTGTCTTTGTTTATGGGCCCCATTGGCGTGGAGCCCCGTTTAATTTTCGGGGGTGTTAGAGACAACCAGTGGAGTCCGCTGCTGTCGGCGTCCACTCTCTTTCCCCTTGTTACAAATAGAGTGTAACAACATGGTTCACCTGTCTTGGTCCCTGCCTGGGACACATCTTAATAACCCCAGTATCATATTGCACTAGGATTATGTGTTGCCCATAGCCATAAATTCGTGTGAGATGGACATCCAGTCTTTACGGCTTGTCCCCACCCCATGGATTTCTATTGTTAAAGATATTCAGAATGTTTCATTCCTACACTAGTATTTATTGCCCAAGGGGTTTGTGAGGGTTATATTGGTGTCATAGCACAATGCCACCACTGAACCCCCCGTCCAAATTTTATTCTGGGGGCGTCACCTGAAACCTTGTTTTCGAGCACCTCACATACACCTTACTGTTCACAACTCAGCAGTTATTCTATTAGCTAAACGAAGGAGAATGAAGAAGCAGGCGAAGATTCAGGAGAGTTCACTGCCCGCTCCTTGATCTTCAGCCACTGCCCTTGTGACTAAAATGGTTCACTACCCTCGTGGAATCCTGACCCCATGTAAATAAAACCGTGACAGCTCATGGGGTGGGAGATATCGCTGTTCCTTAGGACCCTTTTACTAACCCTAATTCGATAGCATATGCTTCCCGTTGGGTAACATATGCTATTGAATTAGGGTTAGTCTGGATAGTATATACTACTACCCGGG" 
COMMENT              "Fragment of pREP4FLAG; Length: 772" 0 (CStringList) 0) 
COMMENT         #45=(COligo "EYFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pEYFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #46=(COligo "f1 ori" 
COMMENT              "ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT" 
COMMENT              "Fragment of pAS2; Length: 456" 0 (CStringList) 0) 
COMMENT         #47=(COligo "fd terminator" 
COMMENT              "TAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGAGATTTTCAACGTGAAAAAATTATTATTCGCAATTCC" 
COMMENT              "Fragment of bacteriophage_fd; Length: 82, zoals in pMa58" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #48=(COligo "FLAG epitope" "GAYTAYAARGAYGAYGAYGAYAAR" 
COMMENT              " Fragment of pCAGGS-FLAGmA20; Length: 24" 0 (CStringList) 0) 
COMMENT         #49=(COligo "FLD1 promoter start" 
COMMENT              "GCATGCAGGAATCTCTGGCACGGTGCTAATGGTAGTTATCCAACGGAGCTGAGGTAGTCGATATATCTGGATATGCCGCCTATAGGATAAAAACAGGAGA" 
COMMENT              "Fragment of pFLDalpha; Length: 100" 0 (CStringList) 0) 
COMMENT         #50=(COligo "FLD1 promoter stop" 
COMMENT              "TTTAGAGCCTTACCTGATCGCAATCAGGATTTCACTACTCATATAAATACATCGCTCAAAGCTCCAACTTTGCTTGTTCATACAATTCTTGATATTCACA" 
COMMENT              "Fragment of pFLDalpha; Length: 100" 0 (CStringList) 0) 
COMMENT         #51=(COligo "FLP terminator" 
COMMENT              "GTACGCATTTAAGCATAAACACGCACTATGCCGTTCTTCTCATGTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTGTATGTGC" 
COMMENT              "Fragment of pSCGALyEGFP1 compl; Length: 105" 0 (CStringList) 0) 
COMMENT         #52=(COligo "GAL1 promoter" 
COMMENT              "AAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 384" 0 (CStringList) 0) 
COMMENT         #53=(COligo "GAL10/CYC1 promoter" 
COMMENT              "ATAACCATAAAAGCTAGTATTGTAGAATCTTTATTGTTCGGAGCAGTGCGGCGCGAGGCACATCTGCGTTTCAGGAACGCGACCGGTGAAGACGAGGACGCACGGAGGAGAGTCTTCCTTCGGAGGGCTGTCACCCGCTCGGCGGCTTCTAATCCGTACTTCAATATAGCAATGAGCAGTTAAGCGTATTACTGAAAGTTCCAAAGAGAAGGTTTTTTTAGGCTAATCGACCTCGAGCAGATCCGCCAGGCGTGTATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCTCTAAATATTCTTTCCTTATACAT" 
COMMENT              "Fragment of pEMBLyex4; Length: 408" 0 (CStringList) 0) 
COMMENT         #54=(COligo "GALad" 
COMMENT              "ATGGATAAAGCGGAATTAATTCCCGAGCCTCCAAAAAAGAAGAGAAAGGTCGAATTGGGTACCGCCGCCAATTTTAATCAAAGTGGGAATATTGCTGATAGCTCATTGTCCTTCACTTTCACTAACAGTAGCAACGGTCCGAACCTCATAACAACTCAAACAAATTCTCAAGCGCTTTCACAACCAATTGCCTCCTCTAACGTTCATGATAACTTCATGAATAATGAAATCACGGCTAGTAAAATTGATGATGGTAATAATTCAAAACCACTGTCACCTGGTTGGACGGACCAAACTGCGTATAACGCGTTTGGAATCACTACAGGGATGTTTAATACCACTACAATGGATGATGTATATAACTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAG" 
COMMENT              "Fragment of pGAD-mCASP-11; Length: 408" 0 (CStringList) 0) 
COMMENT         #55=(COligo "GALbd" 
COMMENT              "ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGATAATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCG" 
COMMENT              "Fragment of pAS3; Length: 441" 0 (CStringList) 0) 
COMMENT         #56=(COligo "GALL promoter" 
COMMENT              "TGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT              "Fragment of p415GALLmBAX; Length: 369" 0 (CStringList) 0) 
COMMENT         #57=(COligo "GAP promoter" 
COMMENT              "CTTTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACGGCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGCCCAGGGATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGACGCATGTCATGAGATTATTGGAAACCACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAATTTATTTGTCCCTATTTCAATCAATTGAACAACTAT" 
COMMENT              "Fragment of pGAPZMFManHDEL; Length: 483" 0 (CStringList) 0) 
COMMENT         #58=(COligo "GST" 
COMMENT              "ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAA" 
COMMENT              "Fragment of pGEXGSTp65(12-317); Length: 654" 0 (CStringList) 0) 
COMMENT         #59=(COligo "GUS start" 
COMMENT              "ATGGTCCGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAACTGTGGAATTGATCAGCGTT" 
COMMENT              "Fragment of pENTR-gus; Length: 100" 0 (CStringList) 0) 
COMMENT         #60=(COligo "GUS stop" 
COMMENT              "CACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATAA" 
COMMENT              "Fragment of pENTR-gus; Length: 100" 0 (CStringList) 0) 
COMMENT         #61=(COligo "HA epitope" "TAYCCNTAYGAYGTNCCNGAYTAYGCN" 
COMMENT              "Fragment of pAS2; Length: 27" 0 (CStringList) 0) 
COMMENT         #62=(COligo "hBID" 
COMMENT              "ATGGACTGTGAGGTCAACAACGGTTCCAGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCTTCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCACTCCCGCTTGGGAAGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCCGGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAGGCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATGTCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGA" 
COMMENT              "Fragment of pAS2hBID; Length: 588" 0 (CStringList) 0) 
COMMENT         #63=(COligo "hCMV-IE enhancer" 
COMMENT              "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCA" 
COMMENT              "Fragment of pCAGGS-FLAGAVPShOMI; Length: 378" 0 (CStringList) 0) 
COMMENT         #64=(COligo "hCMV-IE promoter and enhancer" 
COMMENT              "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT" 
COMMENT              "Fragment of pCDM8; Length: 584" 0 (CStringList) 0) 
COMMENT         #65=(COligo "HDEL-tag" "CAYGAYGARYTN" 
COMMENT              "Fragment of pGAPZScgls2a-HDEL; Length: 12" 0 (CStringList) 0) 
COMMENT         #66=(COligo "hEF1 promoter (1)" 
COMMENT              "GCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACT" 
COMMENT              "Fragment of pEF1-hOMI; Length: 1000" 0 (CStringList) 0) 
COMMENT         #67=(COligo "hEF1 promoter (2)" 
COMMENT              "GAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGA" 
COMMENT              "Fragment of pEF1-hOMI; Length: 144" 0 (CStringList) 0) 
COMMENT         #68=(COligo "His-tag" "CAYCAYCAYCAYCAYCAY" "" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #69=(COligo "HIS3 start" 
COMMENT              "ATGACAGAGCAGAAAGCCCTAGTAAAGCGTATTACAAATGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTGGTCCCCTAGCGATAGAGCACTCGA" 
COMMENT              "Fragment of p413 GAL1; Length: 100" 0 (CStringList) 0) 
COMMENT         #70=(COligo "HIS3 stop" 
COMMENT              "TAGTGAGAGTGCGTTCAAGGCTCTTGCGGTTGCCATAAGAGAAGCCACCTCGCCCAATGGTACCAACGATGTTCCCTCCACCAAAGGTGTTCTTATGTAG" 
COMMENT              "Fragment of p413 GAL1; Length: 100" 0 (CStringList) 0) 
COMMENT         #71=(COligo "HIS4 terminator" 
COMMENT              "TTATTTAGAGATTTTAACTTACATTTAGATTCGATAGATCC" 
COMMENT              "Fragment of pGlycoSwitchM5; Length: 41" 0 (CStringList) 0) 
COMMENT         #72=(COligo "hOMI (1)" 
COMMENT              "ATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGACCCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTCACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACTCTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCTCGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAGATCCTGGACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATGTGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCTGAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCACTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATCC" 
COMMENT              "Fragment of pEF1-hOMI; Length: 1000" 0 (CStringList) 0) 
COMMENT         #73=(COligo "hOMI (2)" 
COMMENT              "CTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTGCTGAACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGA" 
COMMENT              "Fragment of pEF1-hOMI; Length: 377" 0 (CStringList) 0) 
COMMENT         #74=(COligo "HSV-TK polyA" 
COMMENT              "AACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCAC" 
COMMENT              "Fragment of pEYFP-N1; Length: 70" 0 (CStringList) 0) 
COMMENT         #75=(COligo "HSV-TK promoter" 
COMMENT              "CCCCGCCCAGCGTCTTGTCATTGGCGAATTCGAACACGCAGATGCAGTCGGGGCGGCGCGGTCCCAGGTCCACTTCGCATATTAAGGTGACGCGTGTGGCCTCGA" 
COMMENT              "Fragment of p220.2; Length: 105" 0 (CStringList) 0) 
COMMENT         #76=(COligo "htBID" 
COMMENT              "GGCAACCGCAGCAGCCACTCCCGCTTGGGAAGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCCGGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAGGCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATGTCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGA" 
COMMENT              "Fragment of pAS2htBID; Length: 408" 0 (CStringList) 0) 
COMMENT         #77=(COligo "hygromycin (1)" 
COMMENT              "ATGAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCA" 
COMMENT              "Fragment of pREP4FLAG; Length: 1000" 0 (CStringList) 0) 
COMMENT         #78=(COligo "hygromycin (2)" "GCACTCGTCCGAGGGCAAAGGAATAG" 
COMMENT              "Fragment of pREP4FLAG; Length: 26" 0 (CStringList) 0) 
COMMENT         #79=(COligo "intron AG promoter" 
COMMENT              "GTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAGGGCTCGGGGGAAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGAAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAAGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAG" 
COMMENT              "Fragment of pCAGGS-FLAGAVPShOMI; Length: 925" 0 (CStringList) 0) 
COMMENT         #80=(COligo "intron hEF1 promoter" 
COMMENT              "GTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAG" 
COMMENT              "Fragment of pEF1-hOMI; Length: 939" 0 (CStringList) 0) 
COMMENT         #81=(COligo "IRES start" 
COMMENT              "CCCCCCCCCCCCCCCCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCC" 
COMMENT              "Fragment of aaapUHGIRES-lacZ; Length: 100" 0 (CStringList) 0) 
COMMENT         #82=(COligo "IRES stop" 
COMMENT              "CTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAA" 
COMMENT              "Fragment of aaapUHGIRES-lacZ; Length: 100" 0 (CStringList) 0) 
COMMENT         #83=(COligo "kanamycin" 
COMMENT              "ATGAGCCATATTCAACGGGAAACGTCTTGCTCAAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGTTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA" 
COMMENT              "Fragment of pRH001; Length: 816" 0 (CStringList) 0) 
COMMENT         #84=(COligo "lac operator NIET APART AANDUIDEN" "TTGTGAGCGGATAACAATT" 
COMMENT              "Fragment of pCAL-c; Length: 19     SAMEN MET PROMOTER AANDUIDEN! zie T7" 
COMMENT              0 (CStringList) 0) 
COMMENT         #85=(COligo "lac promoter" 
COMMENT              "GCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGG" 
COMMENT              "from pUC18; 84 bp." 0 (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #86=(COligo "lacA" 
COMMENT              "TTGAACATGCCAATGACCGAAAGAATAAGAGCAGGCAAGCTATTTACCGATATGTGCGAAGGCTTACCGGAAAAAAGACTTCGTGGGAAAACGTTAATGTATGAGTTTAATCACTCGCATCCATCAGAAGTTGAAAAAAGAGAAAGCCTGATTAAAGAAATGTTTGCCACGGTAGGGGAAAACGCCTGGGTAGAACCGCCTGTCTATTTCTCTTACGGTTCCAACATCCATATAGGCCGCAATTTTTATGCAAATTTCAATTTAACCATTGTCGATGACTACACGGTAACAATCGGTGATAACGTACTGATTGCACCCAACGTTACTCTTTCCGTTACGGGACACCCTGTACACCATGAATTGAGAAAAAACGGCGAGATGTACTCTTTTCCGATAACGATTGGCAATAACGTCTGGATCGGAAGTCATGTGGTTATTAATCCAGGCGTCACCATCGGGGATAATTCTGTTATTGGCGCGGGTAGTATCGTCACAAAAGACATTCCACCAAACGTCGTGGCGGCTGGCGTTCCTTGTCGGGTTATTCGCGAAATAAACGACCGGGATAAGCACTATTATTTCAAAGATTATAAAGTTGAATCGTCAGTTTAA" 
COMMENT              "Fragment of pLacDN10HIFNG; Length: 612" 0 (CStringList) 0) 
COMMENT         #87=(COligo "lacI promoter" 
COMMENT              "GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT" 
COMMENT              "Fragment of pREP4; Length: 78" 0 (CStringList) 0) 
COMMENT         #88=(COligo "lacI start" 
COMMENT              "GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAA" 
COMMENT              "Fragment of p714; Length: 100" 0 (CStringList) 0) 
COMMENT         #89=(COligo "lacI stop" 
COMMENT              "CACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA" 
COMMENT              "Fragment of p714; Length: 100" 0 (CStringList) 0) 
COMMENT         #90=(COligo "lacIq promoter" 
COMMENT              "GACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT" 
COMMENT              "Fragment of p714; Length: 78" 0 (CStringList) 0) 
COMMENT         #91=(COligo "lacUV5 promoter" 
COMMENT              "CACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGG" 
COMMENT              "Fragment of p714; Length: 57" 0 (CStringList) 0) 
COMMENT         #92=(COligo "lacZ RBS" "AATTGTGAGCGGATAACAATTTCACACAGGAAACAGCT" 
COMMENT              "Fragment of pGBF-NLScre; Length: 38" 0 (CStringList) 0) 
COMMENT         #93=(COligo "lacZ start" 
COMMENT              "ATGGGGCCCAAGAAGAAACGCAAAGTGGGGAGCATGGGGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC" 
COMMENT              "Fragment of pBI-G; Length: 100" 0 (CStringList) 0) 
COMMENT         #94=(COligo "lacZ stop" 
COMMENT              "GGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTACAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAA" 
COMMENT              "Fragment of pBI-G; Length: 100" 0 (CStringList) 0) 
COMMENT         #95=(COligo "LEU2 start" 
COMMENT              "ATGTTTGTTCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGAAATCACAGCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTC" 
COMMENT              "Fragment of pSCGALyEGFP1; Length: 100" 0 (CStringList) 0) 
COMMENT         #96=(COligo "LEU2 stop" 
COMMENT              "TTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAA" 
COMMENT              "Fragment of pSCGALyEGFP1; Length: 100" 0 (CStringList) 0) 
COMMENT         #97=(COligo "loxP" "ATAACTTCGTATAGCATACATTATACGAAGTT" 
COMMENT              "Fragment of pCALNL5; Length: 32" 0 (CStringList) 0) 
COMMENT         #98=(COligo "LUC start" 
COMMENT              "ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCGCTAGAGGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACG" 
COMMENT              "Fragment of pBI-GL; Length: 100" 0 (CStringList) 0) 
COMMENT         #99=(COligo "LUC stop" 
COMMENT              "TGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTAA" 
COMMENT              "Fragment of pBI-GL; Length: 100" 0 (CStringList) 0) 
COMMENT         #100=(COligo "luxA (1)" 
COMMENT               "ATGAAGTTTGGAAATATTTGTTTTTCGTATCAACCACCAGGTGAAACTCATAAGCAAGTAATGGATCGCTTTGTTCGGCTTGGTATCGCCTCAGAAGAGGTAGGGTTTGATACATATTGGACCTTAGAACATCATTTTACAGAGTTTGGTCTTACGGGAAATTTATTTGTTGCTGCGGCTAACCTGTTAGGAAGAACTAAAACATTAAATGTTGGCACTATGGGGGTTGTTATTCCGACAGCACACCCAGTTCGACAGTTAGAAGACGTTTTATTATTAGATCAAATGTCGAAAGGTCGTTTTAATTTTGGAACCGTTCGAGGGCTATACCATAAAGATTTTCGAGTATTTGGTGTTGATATGGAAGAGTCTCGAGCAATTACTCAAAATTTCTACCAGATGATAATGGAAAGCTTACAGACAGGAACCATTAGCTCTGATAGTGATTACATTCAATTTCCTAAGGTTGATGTATATCCCAAAGTGTACTCAAAAAATGTACCAACCTGTATGACTGCTGAGTCCGCAAGTACGACAGAATGGCTAGCAATACAAGGGCTACCAATGGTTCTTAGTTGGATTATTGGTACTAATGAAAAAAAAGCACAGATGGAACTCTATAATGAAATTGCGACAGAATATGGTCATGATATATCTAAAATAGATCATTGTATGACTTATATTTGTTCTGTTGATGATGATGCACAAAAGGCGCAAGATGTTTGTCGGGAGTTTCTGAAAAATTGGTATGACTCATATGTAAATGCGACCAATATCTTTAATGATAGCAATCAAACTCGTGGTTATGATTATCATAAAGGTCAATGGCGTGATTTTGTTTTACAAGGACATACAAACACCAATCGACGTGTTGATTATAGCAATGGTATTAACCCTGTAGGCACTCCTGAGCAGTGTATTGAAATCATTCAACGTGATATTGATGCAACGGGTATTACAAACATTACATGCGGATTTGAAGCTAATGGAACTGAAGATG" 
COMMENT               "Fragment of pJE202; Length: 1000" 0 (CStringList) 0) 
COMMENT         #101=(COligo "luxA (2)" 
COMMENT               "AAATAATTGCTTCCATGCGACGCTTTATGACACAAGTCGCTCCTTTCTTAAAAGAACCTAAATAA" 
COMMENT               "Fragment of pJE202; Length: 65" 0 (CStringList) 0) 
COMMENT         #102=(COligo "luxB" 
COMMENT               "ATGAAATTTGGATTATTTTTTCTAAACTTTCAGAAAGATGGAATAACATCTGAAGAAACGTTGGATAATATGGTAAAGACTGTCACGTTAATTGATTCAACTAAATATCATTTTAATACTGCCTTTGTTAATGAACATCACTTTTCAAAAAATGGTATTGTTGGAGCACCTATTACCGCAGCTGGTTTTTTATTAGGGTTAACAAATAAATTACATATTGGTTCATTAAATCAAGTAATTACCACCCATCACCCTGTACGTGTAGCAGAAGAAGCCAGTTTATTAGATCAAATGTCAGAGGGACGCTTCATTCTTGGTTTTAGTGACTGCGAAAGTGATTTCGAAATGGAATTTTTTAGACGTCATATCTCATCAAGGCAACAACAATTTGAAGCATGCTATGAAATAATTAATGACGCATTAACTACAGGTTATTGTCATCCCCAAAACGACTTTTATGATTTTCCAAAGGTTTCAATTAATCCACACTGTTACAGTGAGAATGGACCTAAGCAATATGTATCCGCTACATCAAAAGAAGTCGTCATGTGGGCAGCGAAAAAGGCACTGCCTTTAACATTTAAGTGGGAGGATAATTTAGAAACCAAAGAACGCTATGCAATTCTATATAATAAAACAGCACAACAATATGGTATTGATATTTCGGATGTTGATCATCAATTAACTGTAATTGCGAACTTAAATGCTGATAGAAGTACGGCTCAAGAAGAAGTGAGAGAATACTTAAAAGACTATATCACTGAAACTTACCCTCAAATGGACAGAGATGAAAAAATTAACTGCATTATTGAAGAGAATGCAGTTGGGTCTCATGATGACTATTATGAATCGACAAAATTAGCAGTGGAAAAAACAGGGTCTAAAAATATTTTATTATCCTTTGAATCAATGTCCGATATTAAAGATGTAAAAGATATTATTGATATGTTGAACCAAAAAATCGAAATGAATTTACCATAA" 
COMMENT               "Fragment of pJE202; Length: 981" 0 (CStringList) 0) 
COMMENT         #103=(COligo "luxC (1)" 
COMMENT               "ATGAATAAATGTATTCCAATGATAATTAATGGAATGATTCAAGATTTTGATAATTATGCATATAAAGAAGTTAAACTAAATAATGATAATAGAGTAAAATTATCTGTCATTACTGAAAGTTCAGTTTCAAAAACATTAAATATCAAAGATAGAATTAATCTAAATTTAAATCAGATTGTGAATTTTTTATATACCGTTGGTCAACGATGGAAAAGTGAAGAATATAATCGGCGACGAACCTATATCCGTGAGTTAAAAACATATCTTGGTTATTCTGATGAAATGGCAAGATTAGAAGCGAATTGGATTGCAATGTTATTGTGCTCTAAAAGTGCTTTGTATGACATTGTTAATTATGATTTGGGCTCTATACACGTATTAGATGAATGGCTTCCACGTGGTGATTGCTATGTTAAAGCACAACCGAAAGGTGTTTCTGTTCACTTGTTAGCTGGTAATGTTCCATTATCAGGAGTGACATCTATTTTGCGTGCTATTTTAACAAAAAATGAGTGCATTATTAAAACTTCGTCTTCAGATCCTTTTACTGCAAACGCTTTAGTTTCCAGTTTTATTGATGTTAATGCAGACCATCCAATAACCAAATCAATGTCTGTTATGTATTGGCCGCATGATGAAGATATGACTCTATCTCAAAGAATAATGAATCATGCCGACGTGGTTATTGCTTGGGGTGGAGACGAGGCGATTAAATGGGCGGTAAAATATTCACCACCGCATGTCGATATTCTGAAATTTGGACCAAAGAAAAGCTTAAGTATTATTGAAGCTCCTAAAGATATAGAAGCCGCAGCAATGGGGGTTGCTCATGATATTTGTTTCTATGACCAGCAAGCCTGCTTCTCTACTCAAGACGTTTATTATATAGGAGATAATTTACCTTTATTTTTAAATGAACTTGAAAAACAGCTAGATCGATACGCGAAAATTTTACCAAAAGGTTCAAATAGTTTTGATGAAAAAGCGGCGTTTACTCTTA" 
COMMENT               "Fragment of pJE202; Length: 1000" 0 (CStringList) 0) 
COMMENT         #104=(COligo "luxC (2)" 
COMMENT               "CTGAAAAAGAAAGTCTATTTGCTGGATATGAAGTGAGAAAGGGAGATAAGCAAGCTTGGTTAATAGTCGTATCACCTACAAATAGCTTTGGAAATCAACCGCTATCACGAAGTGTGTATGTTCATCAAGTATCTGATATTAAAGAGATAATTCCTTTTGTTAATAAAAATAGAACACAAACTGTTTCTATTTATCCTTGGGAAGCGTCATTAAAATATCGAGATAAATTAGCAAGAAGTGGAGTTGAAAGAATTGTTGAATCAGGCATGAATAATATTTTCAGAGTTGGAGGGGCTCATGATTCATTATCTCCTCTCCAGTACCTAGTTAGGTTTGTATCGCATGAGAGACCATTTAATTATACGACAAAAGATGTTGCGGTTGAAATCGAACAAACACGTTACTTAGAGGAAGATAAGTTTTTAGTTTTTGTCCCATAG" 
COMMENT               "Fragment of pJE202; Length: 440" 0 (CStringList) 0) 
COMMENT         #105=(COligo "luxD" 
COMMENT               "ATGAAAGATGAAAGTGCTTTTTTTACGATTGATCACATCATCAAGCTTGATAATGGTCAGTCTATCCGAGTTTGGGAAACACTCCCTAAAAAGAACGTACCAGAGAAAAAACATACAATACTTATTGCTTCGGGTTTTGCTAGAAGAATGGATCATTTTGCAGGTCTTGCTGAGTATTTATCTACTAACGGTTTTCATGTCATTCGCTACGATTCTTTGCATCATGTTGGATTAAGCAGTGGATGTATAAATGAATTTACGATGTCGATTGGAAAAAATAGCCTGCTTACAGTCGTAGATTGGCTTACAGATCATGGTGTCGAACGAATAGGGCTGATTGCTGCTAGTTTGTCAGCGAGAATCGCCTATGAGGTAGTAAATAAAATTAAATTATCATTTTTAATTACGGCCGTAGGTGTCGTTAATCTTAGAGATACATTAGAAAAAGCATTGGAGTATGACTATTTGCAATTACCTATTTCAGAGTTACCAGAAGATCTTGACTTTGAAGGTCATAATTTAGGATCGGAGGTCTTTGTTACAGATTGCTTTAAACATAATTGGGACACGTTAGACTCGACACTTAATAGTGTTAAAGGATTAGCGATTCCATTTATTGCTTTTACTGCAAACGATGATAGTTGGGTAAAGCAAAGTGAAGTTATAGAGCTCATTGATAGCATTGAATCTAGTAATTGTAAGCTCTATTCGCTAATTGGAAGTTCACATGATCTTGGGGAAAATTTGGTTGTATTAAGAAATTTTTATCAATCAGTGACGAAGGCAGCCTTAGCATTAGATGATGGTTTATTGGATTTAGAGATAGACATTATTGAACCTCGATTTGAGGACGTTACAAGTATTACTGTTAAGGAGCGTAGATTAAAAAATGAAATTGAAAATGAATTATTAGAATTGGCTTAA" 
COMMENT               "Fragment of pJE202; Length: 924" 0 (CStringList) 0) 
COMMENT         #106=(COligo "luxE (1)" 
COMMENT               "ATGACTGTCCATACTGAATATAAAAGAAATCAAATCATTGCTAGTTCAGAAATTGATGATCTTATCTTTATGACGAAACCACAAGAGTGGTCATTTGAAGAGCAAAAAGAAATACGGGATAAATTAGTTCGTGAGGCTTTTTATTTTCACTACAATAGAAATGAAGAATATAGAAATTATTGTATCAATCAGCATGTGAGTGATAATTTACACACTATTGATGAAATACCCGTGTTTCCAACATCTGTTTTTAAATATAAGAAATTACATACTGTCACAGCCGAGGACATTGAAAATTGGTATACAAGTAGTGGAACTCGTGGAGTAAAAAGTCATATTGCACGTGATCGTCTTAGCATTGAACGCTTGCTTGGTTCTGTCAACTTCGGAATGAAATACGTTGGAGATTGGTTTGAGCATCAAATGGAATTGATAAATTTAGGACCAGATAGATTCAATACAAATAATATTTGGTTTAAATATGTCATGAGTTTGGTCGAGTTACTTTATCCGACTGAATTTACAGTTGATAATGACAAAATAGATTTTGAAAAAACAGTAAAACATCTATTTAGAATTAAGAATAGTAAAAAAGACATTTGCTTAATTGGGCCACCATTTTTTGTGTATCTTTTGTGCCAATATATGAAAGAAAACAATATTGAATTTAAAGGAGGAGATAGAGTACATATTATTACTGGTGGAGGATGGAAATCTAATCAGAATGACTCTTTAGATCGTGCTGATTTTAATCAATTATTAATGGATACTTTCCAACTCGACAAGATTAATCAAATTAGAGATACCTTTAATCAAGTTGAGCTTAATACTTGTTTTTTTGAAGATGAATTTCAAAGAAAACATGTTCCACCGTGGGTATATGCTCGGGCTCTTGATCCTGAAACCTTGAAACCCGTAGCAGATGGTGAGATCGGGTTGTTAAGTTATATGGACGCCTCATCAACTGCTTACCCTGCTTTTATTGTTACTGATGATATCG" 
COMMENT               "Fragment of pJE202; Length: 1000" 0 (CStringList) 0) 
COMMENT         #107=(COligo "luxE (2)" 
COMMENT               "GTATTGTAAAAGAAATTAGAGAACCAGATCCTTACCCAGGGGTAACTGTTGAGATTGTTCGGCGCTTAAATACACGTGCGCAAAAAGGATGCGCGCTCTCTATGGCTAATGTCATACAAAAGAATATCAAGGATTAA" 
COMMENT               "Fragment of pJE202; Length: 137" 0 (CStringList) 0) 
COMMENT         #108=(COligo "luxG" 
COMMENT               "ATGATTGTTGATGGTAGAGTTTCAAAAATAGTATTAGCGTCAATAAGAAATAATATATATAAGGTATTTATTACTGTAAATTCACCAATAAAGTTCATCGCTGGACAATTTGTAATGGTCACGATTAATGGGAAAAAATGCCCTTTTTCAATTGCGAATTGCCCGACAAAAAATTACGAAATAGAATTGCATATTGGTAGTTCGAATAGAGACTGCTCATTGGATATTATCGAATATTTTGTCGATGCTCTTGTTGAGGAAGTCGCAATTGAGTTAGATGCTCCCCATGGAAACGCTTGGTTACGGTCTGAAAGTAATAACCCATTGCTATTAATTGCGGGAGGTACAGGTTTATCATATATAAATAGCATTCTAACAAATTGCTTAAATAGGAATATACCTCAAGATATTTATCTTTACTGGGGAGTAAAAGACAGTTCTCTTTTGTATGAAGATGAAGAGTTACTAAACTTATCACTAAACAACAAAAACTTTCATTATATTCCTGTTATTGAAGATAAAAGTGAAGAATGGATAGGGAGAAAAGGCACTGTTCTTGATGCTGTCATGGAAGATTTTACTGATCTTACTTATTTTGATATTTATGTTTGTGGACCCTTCATGATGGCTAAAACAGCAAAAGAAAAATTAATTGAAGAGAAAAAAGCAAAGTCAGAACAGATGTTTGCCGATGCTTTTGCATACGTATAA" 
COMMENT               "Fragment of pJE202; Length: 711" 0 (CStringList) 0) 
COMMENT         #109=(COligo "luxI" 
COMMENT               "ATGACTATAATGATAAAAAAATCGGATTTTTTGGCAATTCCATCGGAGGAGTATAAAGGTATTCTAAGTCTTCGTTATCAAGTGTTTAAGCAAAGACTTGAGTGGGACTTAGTTGTAGAAAATAACCTTGAATCAGATGAGTATGATAACTCAAATGCAGAATATATTTATGCTTGTGATGATACTGAAAATGTAAGTGGATGCTGGCGTTTATTACCTACAACAGGTGATTATATGCTGAAAAGTGTTTTTCCTGAATTGCTTGGTCAACAGAGTGCTCCCAAAGATCCTAATATAGTCGAATTAAGTCGTTTTGCTGTAGGTAAAAATAGCTCAAAGATAAATAACTCTGCTAGTGAAATTACAATGAAACTATTTGAAGCTATATATAAACACGCTGTTAGTCAAGGTATTACAGAATATGTAACAGTAACATCAACAGCAATAGAGCGATTTTTAAAGCGTATTAAAGTTCCTTGTCATCGTATTGGAGACAAAGAAATTCATGTATTAGGTGATACTAAATCGGTTGTATTGTCTATGCCTATTAATGAACAGTTTAAAAAAGCAGTCTTAAATTAA" 
COMMENT               "Fragment of pJE202; Length: 582" 0 (CStringList) 0) 
COMMENT         #110=(COligo "luxICDABEG terminator" 
COMMENT               "AGAGAATATAAAAAGCCAGATTATTAATCCGGCTT" 
COMMENT               "Fragment of pJE202; Length: 35" 0 (CStringList) 0) 
COMMENT         #111=(COligo "luxR" 
COMMENT               "ATGAAAAACATAAATGCCGACGACACATACAGAATAATTAATAAAATTAAAGCTTGTAGAAGCAATAATGATATTAATCAATGCTTATCTGATATGACTAAAATGGTACATTGTGAATATTATTTACTCGCGATCATTTATCCTCATTCTATGGTTAAATCTGATATTTCAATTCTAGATAATTACCCTAAAAAATGGAGGCAATATTATGATGACGCTAATTTAATAAAATATGATCCTATAGTAGATTATTCTAACTCCAATCATTCACCAATTAATTGGAATATATTTGAAAACAATGCTGTAAATAAAAAATCTCCAAATGTAATTAAAGAAGCGAAAACATCAGGTCTTATCACTGGGTTTAGTTTCCCTATTCATACGGCTAACAATGGCTTCGGAATGCTTAGTTTTGCACATTCAGAAAAAGACAACTATATAGATAGTTTATTTTTACATGCGTGTATGAACATACCATTAATTGTTCCTTCTCTAGTTGATAATTATCGAAAAATAAATATAGCAAATAATAAATCAAACAACGATTTAACCAAAAGAGAAAAAGAATGTTTAGCGTGGGCATGCGAAGGAAAAAGCTCTTGGGATATTTCAAAAATATTAGGCTGCAGTGAGCGTACTGTCACTTTCCATTTAACCAATGCGCAAATGAAACTCAATACAACAAACCGCTGCCAAAGTATTTCTAAAGCAATTTTAACAGGAGCAATTGATTGCCCATACTTTAAAAATTAA" 
COMMENT               "Fragment of pJE202; Length: 753" 0 (CStringList) 0) 
COMMENT         #112=(COligo "M13 ori" 
COMMENT               "ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT" 
COMMENT               "Fragment of pCS4; Length: 456" 0 (CStringList) 0) 
COMMENT         #113=(COligo "mABIN-2 (1)" 
COMMENT               "ATGTCGTCTGGGGACCCAAGGTCTGGTAGACAGGACGGGGCCCCGCGTGCGGCCGCAGCGCTCTGTGGCCTGTACCACGAGGCCGGCCAGCAACTACAGCGCCTGAAGGATCAGCTGGCCGCGCGTGACGCCCTCATCGCGAGCCTCCGCACCCGCCTCGCGGCTCTGGAAGGGCACACGGCGCCGTCACTCGTGGACGCACTTCTGGATCAGGTGGAGCGCTTCCGTGAGCAGCTGCGACGACAGGAGGAAGGCGCTTCGGAGACCCAGCTGCGGCAGGAAGTTGAAAGACTTACGGAGCGTCTAGAGGAAAAAGAGAGGGAGATGCAACAGCTGATGAGCCAGCCTCAGCATGAGCAAGAGAAGGAGGTAGTCTTGCTTCGGCGAAGTGTGGCAGAGAAGGAGAAAGCCAGGGCCGCCAGTGATGTTCTGTGCCGCTCCTTGGCTGATGAGACCCACCAACTGCGCAGGACATTGGCAGCCACTGCCCACATGTGCCAACATCTGGCCAAATGTCTGGATGAACGACAGTGTGCACAGGGAGACGCTGGGGAGAAAAGCCCTGCTGAGCTAGAGCAAACAAGCAGCGATGCTTCTGGCCAGAGTGTTATTAAGAAGTTACAGGAAGAAAATCGACTGTTAAAACAGAAGGTGACTCATGTAGAAGACCTCAATGCTAAGTGGCAGCGTTATGATGCAAGTAGGGACGAATATGTGAAGGGGTTGCATGCCCAGCTAAAGAGGCGGCAGGTCCCTCTGGAGCCTGAGCTGATGAAGAAGGAGATTTCCCGACTTAACAGACAGTTGGAGGAGAAAATAAGTGACTGTGCGGAAGCAAACCAGGAGCTGACAGCCATGAGGATGTCCCGGGACACTGCGCTGGAGCGAGTGCAGATGCTAGAACAGCAGATTCTTGCTTACAAGGATGACTTCAAATCAGAAAGGGCAGATCGGGAACGAGCGCACAGTAGGATTCAAGAGCTGGAGGAAAAGATCATGT" 
COMMENT               "Fragment of pAS2-mABIN-2new; Length: 1000" 0 (CStringList) 0) 
COMMENT         #114=(COligo "mABIN-2 (2)" 
COMMENT               "CCTTGATGTACCAAGTGTCCCAGAGACAGGACTCCCGGGAGCCAGGACCCTGTCGGATTCATACGGGGAACAAAACTGCCAAGTACTTAGAGATGGATGCACTGGAGCATGTGACCCCTGGCGGCTGGAGGCCTGAGTCTAGGTCCCAACAGATGGAACCTTCTGCAGAGGGTGGGCATGTGTGCACAGCCCAGAGAGGTCAGGGTGACCTTCAGTGCCCTCATTGCCTGCGGTGCTTCAGTGATGAGCAAGGCGAGGCATTCCTCAGGCACCTGTCTGAGTGCTGCCAATGA" 
COMMENT               "Fragment of pAS2-mABIN-2new; Length: 293" 0 (CStringList) 0) 
COMMENT         #115=(COligo "mBAX" 
COMMENT               "ATGGACGGGTCCGGGGAGCAGCTTGGGAGCGGCGGGCCCACCAGCTCTGAACAGATCATGAAGACAGGGGCCTTTTTGCTACAGGGTTTCATCCAGGATCGAGCAGGGAGGATGGCTGGGGAGACACCTGAGCTGACCTTGGAGCAGCCGCCCCAGGATGCGTCCACCAAGAAGCTGAGCGAGTGTCTCCGGCGAATTGGAGATGAACTGGATAGCAATATGGAGCTGCAGAGGATGATTGCTGACGTGGACACGGACTCCCCCCGAGAGGTCTTCTTCCGGGTGGCAGCTGACATGTTTGCTGATGGCAACTTCAACTGGGGCCGCGTGGTTGCCCTCTTCTACTTTGCTAGCAAACTGGTGCTCAAGGCCCTGTGCACTAAAGTGCCCGAGCTGATCAGAACCATCATGGGCTGGACACTGGACTTCCTCCGTGAGCGGCTGCTTGTCTGGATCCAAGACCAGGGTGGCTGGGAAGGCCTCCTCTCCTACTTCGGGACCCCCACATGGCAGACAGTGACCATCTTTGTGGCTGGAGTCCTCACCGCCTCGCTCACCATCTGGAAGAAGATGGGCTGA" 
COMMENT               "Fragment of pRS415GAL1mBAX; Length: 579" 0 (CStringList) 0) 
COMMENT         #116=(COligo "mcPLA2 (1)" 
COMMENT               "ATGTCTTTCATAGATCCTTATCAGCACATTATAGTGGAACACCAGTACTCCCATAAGTTTACTGTTGTGGTTCTACGTGCCACCAAAGTAACCAAGGGGACCTTTGGCGATATGCTGGACACTCCAGATCCTTATGTGGAACTTTTCATCTCTACAACCCCTGACAGCAGGAAGCGAACGAGACACTTCAATAATGATATAAACCCCGTGTGGAATGAGACCTTTGAGTTCATTTTGGATCCTAATCAGGAAAATGTTTTGGAGATCACACTGATGGATGCCAACTACGTCATGGATGAAACCCTAGGCACAGCTACATTCCCTGTATCTTCAATGAAAGTGGGAGAGAAGAAAGAAGTCCCTTTTATTTTCAACCAAGTCACTGAAATGATTCTGGAAATGTCTCTGGAAGTTTGTTCATGCCCAGACCTACGGTTCAGCATGGCACTGTGTGATCAGGAGAAAACTTTCAGACAGCAGAGGAAAGAGAACATAAAAGAGAACATGAAGAAACTTTTGGGTCCAAAAAAGAGTGAGGGGCTTTATTCCACACGTGATGTGCCGGTGGTGGCCATTTTGGGTTCAGGTGGGGGTTTCCGGGCCATGGTGGGATTCTCTGGTGTGATGAAGGCACTGTATGAGTCGGGGATTTTGGACTGTGCTACATACATTGCTGGTCTTTCTGGATCCACATGGTACATGTCAACCTTGTACTCTCACCCCGATTTTCCAGAGAAAGGTCCCGAGGAGATTAATGAAGAGCTAATGAAAAATGTCAGCCACAACCCTCTCTTACTTCTTACACCACAGAAAGTTAAAAGATACGTTGAGTCTTTATGGAAGAAGAAAAGTTCTGGCCAGCCTGTCACCTTTACTGACATCTTTGGGATGCTAATAGGAGAAACACTAATTCAAAATAGGATGAGCATGACCCTGAGTAGTTTGAAGGAAAAGGTCAATGCCGCCCGGTGTCCTTTGCCTCTCTTCACGTGTCTCCACG" 
COMMENT               "Fragment of pEF6-Etag-mcPLA2; Length: 1000" 0 (CStringList) 0) 
COMMENT         #117=(COligo "mcPLA2 (2)" 
COMMENT               "TCAAACCTGATGTGTCAGAGCTGATGTTTGCCGATTGGGTGGAATTTAGTCCATATGAGATTGGCATGGCAAAATATGGTACCTTTATGGCTCCTGACCTATTTGGAAGCAAGTTTTTTATGGGAACAGTTGTAAAAAAATATGAAGAAAACCCCTTGCATTTCTTGATGGGTGTCTGGGGCAGTGCCTTTTCTATACTGTTCAACAGAGTTTTGGGAGTTTCTGGCTCACAGAATAAAGGCTCTACAATGGAAGAGGAATTAGAAAATATTACAGCAAAGCACATCGTGAGTAATGACAGCTCCGACAGTGATGATGAGGCTCAAGGACCCAAAGGCACCGAGAATGAAGAAGCTGAAAAAGAGTACCAAAGCGACAACCAAGCAAGTTGGGTCCATCGGATGCTAATGGCCTTGGTGAGCGACTCGGCTTTATTCAATACCCGAGAAGGACGTGCCGGAAAGGTGCATAACTTCATGCTGGGCTTGAATCTCAACACATCATATCCACTGTCTCCCCTGAGAGACTTCAGCTCTCAGGATTCCTTCGATGACGAGCTCGACGCAGCGGTAGCAGATCCAGATGAATTTGAACGAATATATGAACCACTGGATGTCAAAAGTAAGAAGATTCATGTGGTAGATAGTGGGCTCACATTTAACCTGCCGTATCCCTTGATTCTGCGACCTCAGAGAGGTGTGGATCTTATCATCTCCTTTGACTTTTCTGCAAGGCCGAGTGACACCAGTCCCCCTTTCAAGGAACTTCTGCTTGCAGAGAAGTGGGCGAAAATGAACAAGCTTCCCTTTCCAAAGATCGATCCTTATGTGTTTGATCGGGAAGGATTAAAGGAATGCTATGTTTTTAAACCTAAGAATCCTGATGTGGAGAAGGATTGCCCAACCATTATCCACTTTGTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCCCCAGGTGTTCTAAGGGAAACCAAAGAAGAGAAAGAAATCGCTGACTT" 
COMMENT               "Fragment of pEF6-Etag-mcPLA2; Length: 1000" 0 (CStringList) 0) 
COMMENT         #118=(COligo "mcPLA2 (3)" 
COMMENT               "TGACATTTTTGATGACCCCGAATCGCCATTTTCAACCTTCAACTTTCAGTATCCCAATCAAGCATTCAAAAGGCTGCATGATCTGATGTACTTCAACACACTGAACAACATTGATGTGATAAAGGATGCCATTGTTGAGAGCATTGAATACAGAAGACAGAACCCATCTCGTTGCTCTGTTTCCCTCAGTAATGTTGAAGCAAGAAAATTCTTCAATAAGGAGTTTCTAAGTAAACCCACTGTGTAA" 
COMMENT               "Fragment of pEF6-Etag-mcPLA2; Length: 247" 0 (CStringList) 0) 
COMMENT         #119=(COligo "mDHFR" 
COMMENT               "ATGGTTCGACCATTGAACTGCATCGTCGCCGTGTCCCAAAATATGGGGATTGGCAAGAACGGAGACCTACCCTGGCCTCCGCTCAGGAACGAGTTCAAGTACTTCCAAAGAATGACCACAACCTCTTCAGTGGAAGGTAAACAGAATCTGGTGATTATGGGTAGGAAAACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGACAGAATTAATATAGTTCTCAGTAGAGAACTCAAAGAACCACCACGAGGAGCTCATTTTCTTGCCAAAAGTTTGGATGATGCCTTAAGACTTATTGAACAACCGGAATTGGCAAGTAAAGTAGACATGGTTTGGATAGTCGGAGGCAGTTCTGTTTACCAGGAAGCCATGAATCAACCAGGCCACCTTAGACTCTTTGTGACAAGGATCATGCAGGAATTTGAAAGTGACACGTTTTTCCCAGAAATTGATTTGGGGAAATATAAACTTCTCCCAGAATACCCAGGCGTCCTCTCTGAGGTCCAGGAGGAAAAAGGCATCAAGTATAAGTTTGAAGTCTACGAGAAGAAAGACTAA" 
COMMENT               "Fragment of pSCUDMFE6L2; Length: 564" 0 (CStringList) 0) 
COMMENT         #120=(COligo "mob'" 
COMMENT               "ATGGCGGCATACGCGATCATGCGATGCAAGAAGCTGGCGAAAATGGGCAACGTGGCGGCCAGTCTCAAGCACGCCTACCGCGAGCGCGAGACGCCCAACGCTGACGCCAGCAGGACGCCAGAGAACGAGCACTGGGCGGCCAGCAGCACCGATGAAGCGATGGGCCGACTGCGCGAGTTGCTGCCAGAGAAGCGGCGCAAGGACGCTGTGTTGGCGGTCGAGTACGTCATGACGGCCAGCCCGGAATGGTGGAAGTCGGCCAGCCAAGAACAGCAGGCGGCGTTCTTCGAGAAGGCGCACAAGTGGCTGGCGGACAAGTACGGGGCGGATCGCATCGTGACGGCCAGCATCCACCGTGACGAAACCAGCCCGCACATGACCGCGTTCGTGGTGCCGCTGACGCAGGACGGCAGGCTGTCGGCCAAGGAGTTCATCGGCAACAAAGCGCAGATGACCCGCGACCAGACCACGTTTGCGGCCGCTGTGGCCGATCTAGGGCTGCAACGGGGCATCGAGGGCAGCAAGGCACGTCACACGCGCATTCAGGCGTTCTACGAGGCCCTGGAGCGGCCACCAGTGGGCCACGTCACCATCAGCCCGCAAGCGGTCGAGCCACGCGCCTATGCACCGCAGGGATTGGCCGAAAAGCTGGGAATCTCAAAGCGCGTTGAGACGCCGGAAGCCGTGGCCGACCGGCTGACAAAAGCGGTTCGGCAGGGGTATGAGCCTGCCCTACAGGCCGCCGCAGGAGCGCGTGAGATGCGCAAGAAGGCCGATCAAGCCCAAGAGACGGCCCGAGACCTTCGGGAGCGCCTGAAGCCCGTTCTGGACGCCCTGGGGCCGTTGAATCGGGATATGCAGGCCAAGGCCGCCGCGATCATCAAGGCCGTGGGCGAAAAGCTGCTGACGGAACAGCGGGAAGTCCAGCGCCAGAAACAGGCCCAGCGCCAGCAGGAACGCGGGCGCG" 
COMMENT               "incomplete but functional mobilization gene; 6 C-terminal codons missing" 
COMMENT               0 (CStringList) 0) 
COMMENT         #121=(COligo "mPGK1 promoter" 
COMMENT               "TTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGA" 
COMMENT               "Fragment of pPNEcad; Length: 508" 0 (CStringList) 0) 
COMMENT         #122=(COligo "Myc epitope" "GARCARAARYTNATHWSNGARGARGAYYTN" 
COMMENT               "Fragment of MYC-TAG; Length: 30" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #123=(COligo "neomycin" 
COMMENT               "ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA" 
COMMENT               "Fragment of pCDNA-mCASP-8; Length: 795" 0 (CStringList) 0) 
COMMENT         #124=(COligo "nos promoter start" 
COMMENT               "GATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGATTGAA" 
COMMENT               "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #125=(COligo "nos promoter stop" 
COMMENT               "AGGTCACTATCAGCTAGCAAATATTTCTTGTCAAAAATGCTCCACTGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAA" 
COMMENT               "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #126=(COligo "ocs terminator start" 
COMMENT               "CTGCTTTAATGAGATATGCGAGACGCCTATGATCGCATGATATTTGCTTTCAATTCTGTTGTGCACGTTGTAAAAAACCTGAGCATGTGTAGCTCAGATC" 
COMMENT               "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #127=(COligo "ocs terminator stop" 
COMMENT               "GTGGGCCCCCAAATGAAGTGCAGGTCAAACCTTGACAGTGACGACAAATCGTTGGGCGGGTCCAGGGCGAATTTTGCGACAACATGTCGAGGCTCAGCAG" 
COMMENT               "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #128=(COligo "p15A ori start" 
COMMENT               "GGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGT" 
COMMENT               "Fragment of pACYC177; Length: 100" 0 (CStringList) 0) 
COMMENT         #129=(COligo "p15A ori stop" 
COMMENT               "GTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTT" 
COMMENT               "Fragment of pACYC177; Length: 100" 0 (CStringList) 0) 
COMMENT         #130=(COligo "parA" 
COMMENT               "TTGAAGGTCGCCCGCATCTACCTGCGCGCCAGTACGGACGAGCAGAATCTTGAACGCCAGGAGAGCCTTGTAGCGGCCACGCGGGCCGCCGGGTACTACGTCGCCGGCATCTACCGCGAGAAGGCGTCCGGCGCACGCGCCGACCGGCCCGAGCTGCTGCGCATGATCGCGGACCTGCAACCTGGTGAAGTCGTCGTGGAGAGCCTTGTAGCGGCCACGCGGGCCGCCGGGTACTACGTCGCCGGCATCTACCGCGAGAAGGCGTCCGGCGCACGCGCCGACCGGCCCGAGCTGCTGCGCATGATCGCGGACCTGCAACCTGGTGAAGTCGTCGTTGCGGAGAAGATCGACCGCATCAGCCGCTTGCCGTTGGCCGAGGCCGAGCGCCTGGTTGCGTCGATCCGGGCCAAAGGGGCCAAGCTGGCCGTGCCTGGCGTGGTGGACCTGTCGGAGCTGGCCGCCGAGGCGAACGGAGTGGCGAAAATCGTTCTGGAATCCGTCCAGGACATGCTTTTGAAGCTCGCCTTGCAGATGGCCCGCGACGACTACGAGGATCGGCGCGAGCGTCAACGTCAGGGTGTCCAGTTGGCGAAGGCCGCCGGCCGCTACACCGGCCGCAAACGTGACGCCGGCATGCACGACCGCATCATCACGCTTCGCTCCGGCGGATCGAGCATTGCCAAGACGGCCAAGCTGGTCGGATGCAGCCCGAGCCAGGTCAAACGAGTGTGGGCGGCCTGGAACGCGCAGCAGCAAAAATAA" 
COMMENT               "Fragment of pRH001; Length: 762" 0 (CStringList) 0) 
COMMENT         #131=(COligo "parB" 
COMMENT               "ATGAAGCGCCGAAGCTACGCCATGCTGCGCGCCGCTGCCGCGCTGGCCGTCCTGGTCGTTGCCTCGCCGGCATGGGCCGAGCTGCGCGGCGAGGTCGTGCGCATCATCGACGGCGACACCATCGACGTGCTGGTAGACAAGCAGCCGGTGCGCGTGCGCCTGGTGGACATTGACGCGCCGGAAAAGCGGCAAGCCTTCGGCGAACGTGCGCGCCAGGCGCTGGCCGGCATGGTGTTCCGCCGGCACGTCCTGGTCGACGAGAAGGACACCGACCGTTACGGCCGCACGCTGGGCACCGTGTGGGTCAACATGGAGCTGGCCAGCCGGCCGCCGCAGCCGCGCAACGTCAACGCCGCGATGGTTCACCAGGGCATGGCGTGGGCCTATCGCTTCCACGGCCGCGCGGCCGACCCTGAAATGCTGCGGCTCGAACAGGAGGCGCGAGGCAAGCGCGTCGGCCTCTGGTCCGATCCGCACGCCGTCGAGCCGTGGAAATGGCGACGCGAGAGCAACAACCGGAGGGACGAAGGTTGA" 
COMMENT               "Fragment of pRH001; Length: 534" 0 (CStringList) 0) 
COMMENT         #132=(COligo "parC" 
COMMENT               "ATGGGTATACCCAATTTGACCAAGGGGGACGCGATGAGGGCGGCCAAGCACTACCGACAACTTCTATCCATCGACTTCAACATCGAGGCGCTGGCCTTCGTGCCTGGACCCGACGGCACACGCGGCCGGCGCATCCACGTCCTGGGGCGCGAGGTCCGCGACCGGCCCGGCCTGGTCGAGTACCTTTCGCCGGCGTTCGGCTCGCGGGTGGCGCTGGACGGCTACTGCAAGGCCAATTTCGATGCAGTGCTGCACCTGGCGTACCCCGATCATCAGCAATGGGGCCACGCATGA" 
COMMENT               "Fragment of pRH001; Length: 294" 0 (CStringList) 0) 
COMMENT         #133=(COligo "parD" 
COMMENT               "ATGAGCCGCCTGACAATCGACATGACGGACCAGCAGCACCAGAGCCTGAAAGCCCTGGCCGCCTTGCAGGGCAAGACCATTAAGCAATACGCCCTCGAACGTCTGTTCCCCGGTGACGCTGATGCCGATCAGGCATGGCAGGAACTGAAAACCATGCTGGGGAACCGCATCAACGATGGGCTTGCCGGCAAGGTGTCCACCAAGAGCGTCGGCGAAATTCTTGATGAAGAACTCAGCGGGGATCGCGCTTGA" 
COMMENT               "Fragment of pMR10cat; Length: 252" 0 (CStringList) 0) 
COMMENT         #134=(COligo "parE" 
COMMENT               "TTGACGGCCTACATCCTCACGGCTGAGGCCGAAGCCGATCTACGCGGCATCATCCGCTACACGCGCCGGGAGTGGGGCGCGGCGCAGGTGCGCCGCTATATCGCTAAGCTGGAACAGGGCATAGCCAGGCTTGCCGCCGGCGAAGGCCCGTTTAAGGACATGAGCGAACTCTTTCCCGCGCTGCGGATGGCCCGCTGCGAACACCACTACGTTTTTTGCCTGCCGCGTGCGGGCGAACCCGCGTTGGTCGTGGCGATCCTGCATGAGCGCATGGACCTCATGACGCGACTTGCCGACAGGCTCAAGGGCTGA" 
COMMENT               "Fragment of pMR10cat; Length: 312" 0 (CStringList) 0) 
COMMENT         #135=(COligo "pBBR1 ori" 
COMMENT               "ATGGCCACGCAGTCCAGAGAAATCGGCATTCAAGCCAAGAACAAGCCCGGTCACTGGGTGCAAACGGAACGCAAAGCGCATGAGGCGTGGGCCGGGCTTATTGCGAGGAAACCCACGGCGGCAATGCTGCTGCATCACCTCGTGGCGCAGATGGGCCACCAGAACGCCGTGGTGGTCAGCCAGAAGACACTTTCCAAGCTCATCGGACGTTCTTTGCGGACGGTCCAATACGCAGTCAAGGACTTGGTGGCCGAGCGCTGGATCTCCGTCGTGAAGCTCAACGGCCCCGGCACCGTGTCGGCCTACGTGGTCAATGACCGCGTGGCGTGGGGCCAGCCCCGCGACCAGTTGCGCCTGTCGGTGTTCAGTGCCGCCGTGGTGGTTGATCACGACGACCAGGACGAATCGCTGTTGGGGCATGGCGACCTGCGCCGCATCCCGACCCTGTATCCGGGCGAGCAGCAACTACCGACCGGCCCCGGCGAGGAGCCGCCCAGCCAGCCCGGCATTCCGGGCATGGAACCAGACCTGCCAGCCTTGACCGAAACGGAGGAATGGGAACGGCGCGGGCAGCAGCGCCTGCCGATGCCCGATGAGCCGTGTTTTCTGGACGATGGCGAGCCGTTGGAGCCGCCGACACGGGTCACGCTGCCGCGCCGGTAG" 
COMMENT               "" 0 (CStringList) 0) 
COMMENT         #136=(COligo "Pichia URA3" 
COMMENT               "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT               "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #137=(COligo "PL promoter" 
COMMENT               "TCTCTCACCTACCAAACAATGCCCCCCTGCAAAAAATAAATTCATATAAAAAACATACAGATAACCATCTGCGGTGATAAATTATCTCTGGCGGTGTTGACATAAATACCACTGGCGGTGATACTGAGCAC" 
COMMENT               "Fragment of pLT-mCASP-1p30-1; Length: 131" 0 (CStringList) 0) 
COMMENT         #138=(COligo "pMB1 ori" 
COMMENT               "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGC" 
COMMENT               "Fragment of pRH007; Length: 403" 0 (CStringList) 0) 
COMMENT         #139=(COligo "polyoma ori" 
COMMENT               "CTGTTTTTTTTAGTATTAAGCAGAGGCCGGGGACCCCTGGGCCCGCTTACTCTGGAGAAAAAGAAGAGAGGCATTGTAGAGGCTTCCAGAGGCAACTTGTCAAAACAGGACTG" 
COMMENT               "Fragment of pCDM92; Length: 113" 0 (CStringList) 0) 
COMMENT         #140=(COligo "ppMF" 
COMMENT               "ATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCT" 
COMMENT               "Fragment of pGAPZMFManHDEL; Length: 243" 0 (CStringList) 0) 
COMMENT         #141=(COligo "puromycin" 
COMMENT               "ATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGACCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGTGCCCGAAGGACCGCGCGACCTGGTGCATGACCCGCAAGCCCGGTGCCTGA" 
COMMENT               "Fragment of pSVpaz22; Length: 600" 0 (CStringList) 0) 
COMMENT         #142=(COligo "R6K ori" 
COMMENT               "TCTGAAGATCAGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTCAGTGACACAGGAACACTTAACGGCTGACAT" 
COMMENT               "Fragment of pKNG101; Length: 388" 0 (CStringList) 0) 
COMMENT         #143=(COligo "RK2 ori" 
COMMENT               "GGCCGCCGGCGTTGTGGATACCTCGCGGAAAACTTGGCCCTCACTGACAGATGAGGGGCGGACGTTGACACTTGAGGGGCCGACTCACCCGGCGCGGCGTTGACAGATGAGGGGCAGGCTCGATTTCGGCCGGCGACGTGGAGCTGGCCAGCCTCGCAAATCGGCGAAAACGCCTGATTTTACGCGAGTTTCCCACAGATGATGTGGACAAGCCTGGGGATAAGTGCCCTGCGGTATTGACACTTGAGGGGCGCGACTACTGACAGATGAGGGGCGCGATCCTTGACACTTGAGGGGCAGAGTGCTGACAGATGAGGGGCGCACCTATTGACATTTGAGGGGCTGTCCACAGGCAGAAAATCCAGCATTTGCAAGGGTTTCCGCCCGTTTTTCGGCCACCGCTAACCTGTCTTTTAACCTGCTTTTAAACCAATATTTATAAACCTTGTTTTTAACCAGGGCTGCGCCCTGTGCGCGTGACCGCGCACGCCGAAGGGGGGTGCCCCCCCTTCTCGAACCCTCCCGG" 
COMMENT               "Fragment of pRH001; Length: 526" 0 (CStringList) 0) 
COMMENT         #144=(COligo "RK2/RP4 oriT" 
COMMENT               "CGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGG" 
COMMENT               "Fragment of pRH001; Length: 111" 0 (CStringList) 0) 
COMMENT         #145=(COligo "rop" 
COMMENT               "GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA" 
COMMENT               "Fragment of pBR322; Length: 192" 0 (CStringList) 0) 
COMMENT         #146=(COligo "RSV-LTR" 
COMMENT               "GCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACAGGTCTGACATGGATTGGACGAACCACTGAATTCCGCATTGCAGAGATAATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCT" 
COMMENT               "Fragment of pRcRSVp65; Length: 183" 0 (CStringList) 0) 
COMMENT         #147=(COligo "SP6 promoter" "ATTTAGGTGACACTATA" 
COMMENT               "Fragment of pdCS2-MT ; Length: 17" 0 (CStringList) 0) 
COMMENT         #148=(COligo "Strep-tag" "GCTTGGCGTCACCCGCAGTTCGGTGGT" 
COMMENT               "pUChGNTI2: 27 nucleotides" 0 (CStringList) 0) 
COMMENT         #149=(COligo "supF" 
COMMENT               "TCTTTCTCAACGTAACACTTTACAGCGGCGCGTCATTTGATATGATGCGCCCCGCTTCCCGATAAGGGAGCAGGCCAGTAAAAGCATTACCCGTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTCTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCGAAAGAAT" 
COMMENT               "Fragment of pCDM92; Length: 203" 0 (CStringList) 0) 
COMMENT         #150=(COligo "SV40 early promoter" 
COMMENT               "CTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCC" 
COMMENT               "Fragment of pEF6/Myc-HisA; Length: 282" 0 (CStringList) 0) 
COMMENT         #151=(COligo "SV40 ori" 
COMMENT               "GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCKGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTT" 
COMMENT               "Fragment of SV40; Length: 76" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #152=(COligo "SV40 polyA early" 
COMMENT               "AATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTT" 
COMMENT               "Fragment of pdEYFP; Length: 97" 0 (CStringList) 0) 
COMMENT         #153=(COligo "synthetic EM7 promoter" 
COMMENT               "GTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACC" 
COMMENT               "Fragment of pPICZTS; Length: 66, as in the pEF6 plasmids" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #154=(COligo "T1 terminator" 
COMMENT               "ATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT" 
COMMENT               "Fragment of pDONR201; Length: 44" 0 (CStringList) 0) 
COMMENT         #155=(COligo "T2 terminator" "AGAAGGCCATCCTGACGGATGGCCTTTT" 
COMMENT               "Fragment of pDONR201; Length: 28" 0 (CStringList) 0) 
COMMENT         #156=(COligo "T3 promoter" "AATTAACCCTCACTAAA" 
COMMENT               "Fragment of pLT-mCASP-14" 0 (CStringList) 0) 
COMMENT         #157=(COligo "T7 promoter" "TAATACGACTCACTATA" 
COMMENT               "Fragment of pGEM-TAX1BP1-S; Length: 17" 0 (CStringList) 0) 
COMMENT         #158=(COligo "T7 promoter with lac operator" 
COMMENT               "TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCC" 
COMMENT               "Fragment of pCAL-c; Length: 44" 0 (CStringList) 0) 
COMMENT         #159=(COligo "T7 RBS" 
COMMENT               "GGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAT" 
COMMENT               "Fragment of pLT10Bgal; Length: 63" 0 (CStringList) 0) 
COMMENT         #160=(COligo "T7 terminator" 
COMMENT               "CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG" 
COMMENT               "Fragment of pGBKT7; Length: 48" 0 (CStringList) 0) 
COMMENT         #161=(COligo "tac promoter" "GTTGACAATTAATCATCGGCTCGTATAATGTGTGG" 
COMMENT               "Fragment of pGEX5X1; Length: 35" 0 (CStringList) 0) 
COMMENT         #162=(COligo "TEF1 E. gossypii promoter" 
COMMENT               "GGTTGTTTATGTTCGGATGTGATGTGAGAACTGTATCCTAGCAAGATTTTAAAAGGAAGTATATGAAAGAAGAACCTCAGTGGCAAATCCTAACCTTTTATATTTCTCTACAGGGGCGCGGCGTGGGGACAATTCAACGCGTCTGTGAGGGGAGCGTTTCCCTGCTCGCAGGTCTGCAGCGAGGAGCCGTAATTTTTGCTTCGCGCCGTGCGGCCATCAAAATGTATGGATGCAAATGATTATACATGGGGATGTATGGGCTAAATGTACGGGCGACAGTCACATCATGCCCCTGAGCTGCGCACGTCAAGACTGTCAAGGAGGGTATTCTGGGCCTCCATGTC" 
COMMENT               "E. gossypii TEF1alpha promoter" 0 (CStringList) 0) 
COMMENT         #163=(COligo "TEF1 promoter" 
COMMENT               "CCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTTCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAATTTTTTTTTTTAGTTTTTTTCTCTTTCAGTGACCTCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGTTCATTAGAAAGA" 
COMMENT               "Fragment of pGlycoSwitchM8; Length: 406" 0 (CStringList) 0) 
COMMENT         #164=(COligo "TEF1 terminator start" 
COMMENT               "AGTAACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATT" 
COMMENT               "Fragment of Eremothecium gossypii TEF1; Length: 100" 0 
COMMENT               (CStringList "nagekeken 06/2005 AO") 0) 
COMMENT         #165=(COligo "TEF1 terminator stop" 
COMMENT               "ATATTTTTTTTGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG" 
COMMENT               "Fragment of Eremothecium gossypii TEF1; Length: 100" 0 
COMMENT               (CStringList "nagekeken 06/2005 AO") 0) 
COMMENT         #166=(COligo "TET start" 
COMMENT               "ATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGG" 
COMMENT               "Fragment of pBR322; Length: 100" 0 (CStringList) 0) 
COMMENT         #167=(COligo "TET stop" 
COMMENT               "CACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGA" 
COMMENT               "Fragment of pBR322; Length: 100" 0 (CStringList) 0) 
COMMENT         #168=(COligo "thrombin recognition site" "CTGGTTCCGCGTGGATCC" 
COMMENT               "Fragment of pGEX-4T2; Length: 18" 0 (CStringList) 0) 
COMMENT         #169=(COligo "TRP1" 
COMMENT               "ATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTAGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCCGGTTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAAATAGTTCAGGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGATGTTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGTGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAG" 
COMMENT               "Fragment of pAS2; Length: 675" 0 (CStringList) 0) 
COMMENT         #170=(COligo "URA3" 
COMMENT               "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT               "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0) 
COMMENT         #171=(COligo "V5 epitope" "GGNAARCCNATHCCNAAYCCNYTNYTNGGNYTNGAYWSNACN" 
COMMENT               "length: 42 bp" 0 (CStringList) 0) 
COMMENT         #172=(COligo "VSV epitope" "TACACCGACATCGAGATGAACCGGTTGGGCAAG" 
COMMENT               "Fragment of pCR3VSV-CED-4; Length: 33" 0 (CStringList) 0) 
COMMENT         #173=(COligo "XGPRT" 
COMMENT               "ATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCTTAAAGTGCTGAAACGCGCAGAAGGCGATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAA" 
COMMENT               "Fragment of pDelta1; Length: 459" 0 (CStringList) 0) 
COMMENT         #174=(COligo "Xpress epitope" "GAYYTNTAYGAYGAYGAYGAYAAR" 
COMMENT               "Fragment of pYD1TSE ; Length: 24" 0 (CStringList) 0) 
COMMENT         #175=(COligo "zeocin" 
COMMENT               "ATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGA" 
COMMENT               "Fragment of pGAPZMFManHDEL; Length: 375" 0 (CStringList) 0) 
COMMENT         #176=(COligo "\337-globin polyA" 
COMMENT               "GATCTTTTTCCCTCTGCCAAAAATTATGGGGACATCATGAAGCCCCTTGAGCATCTGACTTCTGGSTAATAAAGGAAATTTATTTTCATTGC" 
COMMENT               "Fragment of pBI-G; Length: 92" 0 (CStringList) 0)) 1 "{(0,1),2}" 
COMMENT        0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 4924 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #177=(CFSignal (CObList) "T7 terminator" 43 0 1 404 451 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(404..451)") 
COMMENT        #178=(CFSignal (CObList) "T710 fragment" 4 0 1 803 835 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(803..835)") 
COMMENT        #179=(CFSignal (CObList) "5' UTR of phage T7 gene 10, incl. RBS" 32 0 1 
COMMENT              836 898 0 (CStringList) (CStringList) 1 1 1 1 
COMMENT              "complement(836..898)") 
COMMENT        #180=(CFSignal (CObList) "T7 promoter" 30 0 1 899 915 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(899..915)") 
COMMENT        #181=(CFSignal (CObList) "pMB1 ori" 33 0 1 2913 3315 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(2913..3315)") 
COMMENT        #182=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3856 4716 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "complement(3856..4716)") 
COMMENT        #183=(CFSignal (CObList) "rop" 21 0 0 2476 2667 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "2476..2667") 
COMMENT        #184=(CFSignal (CObList) "His-tag" 22 0 1 777 797 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(777..797)") 
COMMENT        #185=(CFSignal (CObList) "AU1-tag" 22 0 1 753 770 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(753..770)") 
COMMENT        #186=(CFSignal (CObList) "hUBB mutated mature sequence" 4 0 1 516 746 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "complement(516..746)") 
COMMENT        #187=(CFSignal (CObList) "K6R" 62 0 1 729 731 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(729..731)") 
COMMENT        #188=(CFSignal (CObList) "K29R" 62 0 1 660 662 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(660..662)") 
COMMENT        #189=(CFSignal (CObList) "K27R" 62 0 1 666 668 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(666..668)") 
COMMENT        #190=(CFSignal (CObList) "K33R" 62 0 1 648 650 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(648..650)") 
COMMENT        #191=(CFSignal (CObList) "K48R" 62 0 1 603 605 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(603..605)") 
COMMENT        #192=(CFSignal (CObList) "K63R" 62 0 1 558 560 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(558..560)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #193=(CFSignal (CObList) "Last modified: 23-MAY-2011" 2 0 0 0 0 0 
COMMENT              (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #194=(CFSignal (CObList) "LMBP 5907" 2 0 0 0 0 0 (CStringList) 
COMMENT              (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #195=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT              (CObjectList 
COMMENT               #196=(CRefLinePar 
COMMENT                     (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                      "pET3E-His-AU1-hUb-K11" 2) 5 "" 0 4) 
COMMENT               #197=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #198=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "DNA 'pET3E-His-AU1-hUb-K11'" 1) 
COMMENT                       #199=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                             1) 
COMMENT                       #200=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Created: 05/23/11 02:15" 1) 
COMMENT                       #201=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Last Modified: 05/23/11 02:15" 1) 
COMMENT                       #202=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "length: 4924 bp" 1) 
COMMENT                       #203=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "storage type: Basic" 1) 
COMMENT                       #204=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "form: Circular" 1))) "General Description") 
COMMENT               #205=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #206=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Original Source Database: GenBank" 1) 
COMMENT                       #207=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Modification Date in the Original DB: 23-MAY-2011" 
COMMENT                             1))) "Standard Fields") 
COMMENT               #208=(CRefLinePar 
COMMENT                     (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 
COMMENT                     "" 0 0) 
COMMENT               #209=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #210=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                              (CObjectList 
COMMENT                               #211=(CLinePar 
COMMENT                                     (CParagraph 0 (15 #193# 0) 1 2 2 0 180) "" 
COMMENT                                     1))) "Last modified: 23-MAY-2011") 
COMMENT                       #212=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                              (CObjectList 
COMMENT                               #213=(CLinePar 
COMMENT                                     (CParagraph 0 (15 #194# 0) 1 2 2 0 180) "" 
COMMENT                                     1))) "LMBP 5907"))) "Annotations") 
COMMENT               #214=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #215=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #216=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 516 (3 #186# 0) 1 2 2 0 327) 
COMMENT                                      (CObjectList 
COMMENT                                       #217=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 516   End: 746  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #218=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #219=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(516..746)" 1))) 
COMMENT                                     "hUBB mutated mature sequence") 
COMMENT                               #220=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 803 (3 #178# 0) 1 2 2 0 327) 
COMMENT                                      (CObjectList 
COMMENT                                       #221=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 803   End: 835  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #222=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "first 11 codons of phage T7 gene 10" 
COMMENT                                             1) 
COMMENT                                       #223=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #224=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(803..835)" 1))) 
COMMENT                                     "T710 fragment") 
COMMENT                               #225=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 3856 (3 #182# 0) 1 2 2 0 327) 
COMMENT                                      (CObjectList 
COMMENT                                       #226=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 3856  End: 4716 (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #227=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #228=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(3856..4716)" 1))) 
COMMENT                                     "ampicillin resistance"))) "CDS (3 total)") 
COMMENT                       #229=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #230=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 2476 (3 #183# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #231=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 2476  End: 2667" 1) 
COMMENT                                       #232=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #233=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   2476..2667" 1))) "rop"))) 
COMMENT                             "Misc. Feature (1 total)") 
COMMENT                       #234=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #235=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 753 (3 #185# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #236=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 753   End: 770  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #237=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #238=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(753..770)" 1))) 
COMMENT                                     "AU1-tag") 
COMMENT                               #239=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 777 (3 #184# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #240=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 777   End: 797  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #241=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #242=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(777..797)" 1))) 
COMMENT                                     "His-tag"))) "Misc. Marker (2 total)") 
COMMENT                       #243=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #244=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 899 (3 #180# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #245=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 899   End: 915  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #246=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #247=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(899..915)" 1))) 
COMMENT                                     "T7 promoter"))) 
COMMENT                             "Promoter Prokaryotic (1 total)") 
COMMENT                       #248=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #249=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 836 (3 #179# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #250=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 836   End: 898  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #251=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #252=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(836..898)" 1))) 
COMMENT                                     "5' UTR of phage T7 gene 10, incl. RBS"))) 
COMMENT                             "RBS (1 total)") 
COMMENT                       #253=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #254=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 2913 (3 #181# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #255=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 2913  End: 3315 (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #256=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #257=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(2913..3315)" 1))) 
COMMENT                                     "pMB1 ori"))) 
COMMENT                             "Replication Origin (1 total)") 
COMMENT                       #258=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #259=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 404 (3 #177# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #260=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 404   End: 451  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #261=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #262=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(404..451)" 1))) 
COMMENT                                     "T7 terminator"))) "Terminator (1 total)") 
COMMENT                       #263=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 62 (7 62 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #264=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 558 (3 #192# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #265=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 558   End: 560  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #266=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "wt: AAA" 1) 
COMMENT                                       #267=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #268=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(558..560)" 1))) 
COMMENT                                     "K63R") 
COMMENT                               #269=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 603 (3 #191# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #270=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 603   End: 605  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #271=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "wt: AAG" 1) 
COMMENT                                       #272=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #273=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(603..605)" 1))) 
COMMENT                                     "K48R") 
COMMENT                               #274=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 648 (3 #190# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #275=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 648   End: 650  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #276=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "wt: AAA" 1) 
COMMENT                                       #277=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #278=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(648..650)" 1))) 
COMMENT                                     "K33R") 
COMMENT                               #279=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 660 (3 #188# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #280=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 660   End: 662  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #281=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "wt: AAG" 1) 
COMMENT                                       #282=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #283=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(660..662)" 1))) 
COMMENT                                     "K29R") 
COMMENT                               #284=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 666 (3 #189# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #285=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 666   End: 668  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #286=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "wt: AAG" 1) 
COMMENT                                       #287=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #288=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(666..668)" 1))) 
COMMENT                                     "K27R") 
COMMENT                               #289=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 729 (3 #187# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #290=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 729   End: 731  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #291=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "wt: AAG" 1) 
COMMENT                                       #292=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #293=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(729..731)" 1))) 
COMMENT                                     "K6R"))) "Mutation (6 total)"))) 
COMMENT                     "Feature Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #294=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #295=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #296=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #297=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #298=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #299=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #300=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #301=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #302=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #303=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #304=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #305=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #306=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #307=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #308=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #309=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #310=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) #311=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #312=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #313=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #314=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #315=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #316=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #317=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #318=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #319=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #320=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #321=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #322=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #323=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #324=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #325=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #326=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #327=(CWidgetStyle "~Scrap" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #328=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711935 1 5 "@N (@S)" 0) 
COMMENT          #329=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #330=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #331=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 
COMMENT                1 5 "@N (@S)" 0) 
COMMENT          #332=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #333=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #334=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #335=(CWidgetStyle "Intron Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #336=(CWidgetStyle "Misc_Feature Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #337=(CWidgetStyle "Promoter_E Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #338=(CWidgetStyle "Promoter_P Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #339=(CWidgetStyle "Rep_Origin Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #340=(CWidgetStyle "Terminator Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #341=(CWidgetStyle "oriT Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #342=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #343=(CWidgetStyle "Intron" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #344=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #345=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #346=(CWidgetStyle "Label 8" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #347=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #348=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #349=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #350=(CWidgetStyle "Label 12" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #351=(CWidgetStyle "Uniq RSite" 1 (LOGPEN 0 0 16750899) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #352=(CWidgetStyle "Label 13" 1 (LOGPEN 0 0 16763904) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #353=(CWidgetStyle "Label 14" 1 (LOGPEN 0 0 39168) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #354=(CWidgetStyle "Label 15" 1 (LOGPEN 0 0 16750848) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #355=(CWidgetStyle "Marker" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #356=(CWidgetStyle "Marker Label" 1 (LOGPEN 0 0 0) 3 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #357=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 3 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #358=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #359=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 3381504) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #360=(CWidgetStyle "Label 16" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #361=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 16737792) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #362=(CWidgetStyle "Label 17" 1 (LOGPEN 0 0 16737792) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #363=(CWidgetStyle "Label 18" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #364=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #365=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #366=(CWidgetStyle "Label 19" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #367=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 153) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #368=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 10040064) 4 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #369=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #370=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #371=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #372=(CWidgetStyle "RBS Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #373=(CWidgetStyle "Label 22" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #374=(CWidgetStyle "Label 23" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #375=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #376=(CWidgetStyle "Attenuator" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #377=(CWidgetStyle "Shape 15" 1 (LOGPEN 0 0 6684774) 4 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #378=(CWidgetStyle "Promoter_P" 1 (LOGPEN 0 0 6684774) 8 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #379=(CWidgetStyle "Shape 16" 1 (LOGPEN 0 0 6684774) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #380=(CWidgetStyle "Shape 17" 1 (LOGPEN 0 0 0) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #381=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #382=(CWidgetStyle "Label 24" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #383=(CWidgetStyle "Label 25" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #384=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #385=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #386=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #387=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 16763955) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #388=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #389=(CWidgetStyle "Shape 21" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16763955 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #390=(CWidgetStyle "Attenuator Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #391=(CWidgetStyle "5'UTR Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #392=(CWidgetStyle "3'UTR Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #393=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #394=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #395=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #396=(CWidgetStyle "tRNA Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #397=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #398=(CWidgetStyle "Label 31" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #399=(CWidgetStyle "Label 32" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 
COMMENT                1 5 "@N (@S)" 0) 
COMMENT          #400=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #401=(CWidgetStyle "Label 33" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #402=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #403=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #404=(CWidgetStyle "Shape 30" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #405=(CWidgetStyle "Shape 31" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #406=(CWidgetStyle "Label 35" 1 (LOGPEN 0 0 3394560) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #407=(CWidgetStyle "Label 36" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #408=(CWidgetStyle "Label 37" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 8421376 1 5 "@N (@S)" 0) 
COMMENT          #409=(CWidgetStyle "Label 38" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #410=(CWidgetStyle "Label 39" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 65280 1 5 "@N (@S)" 0) 
COMMENT          #411=(CWidgetStyle "Label 40" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 8388736 1 5 "@N (@S)" 0) 
COMMENT          #412=(CWidgetStyle "Label 41" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711935 1 5 "@N (@S)" 0) 
COMMENT          #413=(CWidgetStyle "Label 42" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16776960 1 5 "@N (@S)" 0) 
COMMENT          #414=(CWidgetStyle "Label 43" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 128 1 5 "@N (@S)" 0) 
COMMENT          #415=(CWidgetStyle "Label 44" 1 (LOGPEN 0 0 39321) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #416=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #417=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #418=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 19.4338 -20.0288 40.3368 40.3368 0.01 10 -1 4924 4924 1 0 0) 
COMMENT        #419=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 979924071 100) 
COMMENT              (CObjectList 
COMMENT               #420=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -896 100) 
COMMENT                     (CObjectList 
COMMENT                      #421=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #310# 35561020 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27546 
COMMENT                             6.27346 1 0.0214037) 0.1274) 
COMMENT                      #422=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #304# 539782773 100) 
COMMENT                            (LOGPEN 0 0 3381657) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pET3E-His-AU1-hUb-K11" "@N" 1 0 
COMMENT                            0.37534 0 -10 16.0152 1.56185 Nil) 
COMMENT                      #423=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #303# 539782773 100) 
COMMENT                            (LOGPEN 840989025 540094768 959593013) 1414350624 
COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "4924 bp" "@L bp" 16 
COMMENT                            0 -1.49095 0 -10.8 4.48722 1.80976 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #424=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -896 100) 
COMMENT                     (CObjectList 
COMMENT                      #425=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -183 100) 
COMMENT                            (CObjectList 
COMMENT                             #426=(CScratch 
COMMENT                                   (CWidget 0 (3 #178# 0) 1 2 0 0 #319# 1 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.25144 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #427=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #370# 1413564227 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T710 fragment" 
COMMENT                                   "@N" 1 19.6114 4.65381 0 -10 6.37135 1.11561 
COMMENT                                   #426#) 
COMMENT                             #428=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #182# 0) 1 2 0 0 #306# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.264992 1.36191 1 0.082322) 0.8 1.8 1) 
COMMENT                             #429=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #370# 892355124 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ampicillin resistance" "@N" 1 -13.2752 
COMMENT                                   9.08352 0 -10 8.85048 1.11561 #428#) 
COMMENT                             #430=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #186# 0) 1 2 0 0 #306# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1.0021 
COMMENT                                    5.32278 5.61707 1 0.082322) 0.8 1.8 1) 
COMMENT                             #431=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #370# 959460916 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hUBB mutated mature sequence" "@N" 1 18.3027 
COMMENT                                   13.5529 0 -10 14.0566 1.11561 #430#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #432=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -254 100) 
COMMENT                            (CObjectList 
COMMENT                             #433=(CLine 
COMMENT                                   (CWidget 0 (3 #183# 0) 1 2 0 0 #314# 1007 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 2.87542 
COMMENT                                   3.12003) 
COMMENT                             #434=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #371# 539782773 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "rop" "@N" 1 
COMMENT                                   -3.26696 -12.1963 0 -10 1.53706 1.11561 #433#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #435=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -324 100) 
COMMENT                            (CObjectList 
COMMENT                             #436=(CScratch 
COMMENT                                   (CWidget 0 (3 #184# 0) 1 2 0 0 #369# 0 100) 
COMMENT                                   (LOGPEN 0 4 10040064) 6 1 5.27182 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #437=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #370# 539782773 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "His-tag" "@N" 1 
COMMENT                                   17.9111 6.39614 0 -10 3.19807 1.11561 #436#) 
COMMENT                             #438=(CScratch 
COMMENT                                   (CWidget 0 (3 #185# 0) 1 2 0 0 #369# 0 100) 
COMMENT                                   (LOGPEN 0 4 10040064) 6 1 5.30494 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #439=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #370# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AU1-tag" "@N" 1 
COMMENT                                   17.9697 7.81619 0 -10 3.6939 1.11561 #438#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #440=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -535 100) 
COMMENT                            (CObjectList 
COMMENT                             #441=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #180# 0) 1 2 0 0 #316# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 5.12913 5.2164) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #442=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #370# 2081903016 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 19.0926 2.91148 0 -10 5.3797 1.11561 
COMMENT                                   #441#)) (CObjectList)) 
COMMENT                      #443=(CGroupWidget 
COMMENT                            (CWidget 32 (7 32 0) 1 2 0 0 Nil -816 100) 
COMMENT                            (CObjectList 
COMMENT                             #444=(CLine 
COMMENT                                   (CWidget 0 (3 #179# 0) 1 2 0 0 #313# 1007 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.670712 5.12913 
COMMENT                                   5.20939) 
COMMENT                             #445=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #371# 539782773 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of phage T7 gene 10, incl. RBS" "@N" 
COMMENT                                   1 21.3884 1.34269 0 -10 16.6597 1.11561 #444#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #446=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -956 100) 
COMMENT                            (CObjectList 
COMMENT                             #447=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #181# 0) 1 2 0 0 #305# 546 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    2.04987 2.56329 1 0.082322) 0.8 1.8 1) 
COMMENT                             #448=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #370# 1144468275 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -11.4093 -8.71165 0 -10 4.06577 1.11561 
COMMENT                                   #447#)) (CObjectList)) 
COMMENT                      #449=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -394 100) 
COMMENT                            (CObjectList 
COMMENT                             #450=(CLine 
COMMENT                                   (CWidget 0 (3 #177# 0) 1 2 0 0 #314# 1007 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 5.69861 
COMMENT                                   5.75976) 
COMMENT                             #451=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #397# 539782773 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 terminator" 
COMMENT                                   "@N" 1 11.5206 17.7386 0 -10 5.80115 1.11561 
COMMENT                                   #450#)) (CObjectList)) 
COMMENT                      #452=(CGroupWidget 
COMMENT                            (CWidget 62 (7 62 0) 1 2 0 0 Nil -464 100) 
COMMENT                            (CObjectList 
COMMENT                             #453=(CScratch 
COMMENT                                   (CWidget 0 (3 #187# 0) 1 2 0 0 #319# 1 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.34571 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #454=(CLabel (CWidget 0 (0 0) 1 2 0 0 #395# 0 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "K6R" "@N" 1 
COMMENT                                   13.8185 8.195 0 -10 1.90893 1.06602 #453#) 
COMMENT                             #455=(CScratch 
COMMENT                                   (CWidget 0 (3 #188# 0) 1 2 0 0 #319# 0 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.43362 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #456=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #395# 1145451330 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "K29R" "@N" 1 
COMMENT                                   13.3523 10.7624 0 -10 2.40475 1.06602 #455#) 
COMMENT                             #457=(CScratch 
COMMENT                                   (CWidget 0 (3 #189# 0) 1 2 0 0 #319# 0 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.42597 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #458=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #395# 2082602632 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "K27R" "@N" 1 
COMMENT                                   13.7309 9.49109 0 -10 2.40475 1.06602 #457#) 
COMMENT                             #459=(CScratch 
COMMENT                                   (CWidget 0 (3 #190# 0) 1 2 0 0 #319# 1 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.44891 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #460=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #395# 1144468275 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "K33R" "@N" 1 
COMMENT                                   12.8814 12.1328 0 -10 2.40475 1.06602 #459#) 
COMMENT                             #461=(CScratch 
COMMENT                                   (CWidget 0 (3 #191# 0) 1 2 0 0 #319# 0 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.50624 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #462=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #395# 959591990 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "K48R" "@N" 1 
COMMENT                                   12.1172 14.7994 0 -10 2.40475 1.06602 #461#) 
COMMENT                             #463=(CScratch 
COMMENT                                   (CWidget 0 (3 #192# 0) 1 2 0 0 #319# 0 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.56357 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #464=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #395# 826689090 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "K63R" "@N" 1 
COMMENT                                   11.7299 16.1203 0 -10 2.40475 1.06602 #463#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #465=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -675 100) 
COMMENT                     (CObjectList 
COMMENT                      #466=(CLabel 
COMMENT                            (CWidget 0 (15 #193# 0) 1 2 0 0 #294# 539782773 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 23-MAY-2011" 
COMMENT                            "Last modified: 23-MAY-2011" 65535 0 -5.20616 0 -10 
COMMENT                            11.3792 1.06602 Nil) 
COMMENT                      #467=(CLabel 
COMMENT                            (CWidget 0 (15 #194# 0) 1 2 0 0 #294# 539782773 100) 
COMMENT                            (LOGPEN 0 0 8388608) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 5907" 
COMMENT                            "LMBP 5907" 65535 -0.0495825 -3.52036 0 -10 7.41259 
COMMENT                            1.56185 Nil)) (CObjectList)) 
COMMENT               #468=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -605 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #469=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -746 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1196705607 
COMMENT       (CStringList) 1094795585 1195852099 (CObList)))
FEATURES             Location/Qualifiers
     terminator      complement(404..451)
                     /vntifkey="43"
                     /label=T7\terminator
     CDS             complement(803..835)
                     /vntifkey="4"
                     /label=T710\fragment
                     /note="first 11 codons of phage T7 gene 10"
     RBS             complement(836..898)
                     /vntifkey="32"
                     /label=5'\UTR\of\phage\T7\gene\10,\incl.\RBS
     promoter        complement(899..915)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      complement(2913..3315)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3856..4716)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    2476..2667
                     /vntifkey="21"
                     /label=rop
     misc_marker     complement(777..797)
                     /vntifkey="22"
                     /label=His-tag
     misc_marker     complement(753..770)
                     /vntifkey="22"
                     /label=AU1-tag
     CDS             complement(516..746)
                     /vntifkey="4"
                     /label=hUBB\mutated\mature\sequence
     mutation        complement(729..731)
                     /vntifkey="62"
                     /label=K6R
                     /note="wt: AAG"
     mutation        complement(660..662)
                     /vntifkey="62"
                     /label=K29R
                     /note="wt: AAG"
     mutation        complement(666..668)
                     /vntifkey="62"
                     /label=K27R
                     /note="wt: AAG"
     mutation        complement(648..650)
                     /vntifkey="62"
                     /label=K33R
                     /note="wt: AAA"
     mutation        complement(603..605)
                     /vntifkey="62"
                     /label=K48R
                     /note="wt: AAG"
     mutation        complement(558..560)
                     /vntifkey="62"
                     /label=K63R
                     /note="wt: AAA"
BASE COUNT     1101 a      1355 c      1277 g      1187 t         4 others
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 
      421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 
      481 cagcttcctt tcgggctttg ttagcagccg aattcctacc cacctctgag acgcaggacc 
      541 aggtgcaggg tcgactccct ctggatgttg tagtcagaaa gagtgcggcc atcttccagc 
      601 tgcctgcctg caaagatgag cctctgctgg tcggggggga tgccttccct atcttggatc 
      661 ctggccctca cattttcgat ggtgtcactg ggctccacct ccagagtgat ggtcttgccg 
      721 gtcagggtcc tcacgaagat ctgcatggat ccgatgtagc ggtatgtgtc ccatggatgg 
      781 tgatggtgat gatgcatnnn ngacccattt gctgtccacc agtcatgcta gccatatgta 
      841 tatctccttc ttaaagttaa acaaaattat ttctagaggg aaaccgttgt ggtctcccta 
      901 tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt 
      961 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga 
     1021 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt 
     1081 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc 
     1141 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca 
     1201 taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg 
     1261 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt 
     1321 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc 
     1381 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt 
     1441 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc 
     1501 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc 
     1561 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc 
     1621 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag 
     1681 cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac 
     1741 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt 
     1801 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct 
     1861 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg 
     1921 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg 
     1981 cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg 
     2041 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc 
     2101 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg 
     2161 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt 
     2221 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta 
     2281 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc 
     2341 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt 
     2401 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct 
     2461 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc 
     2521 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca 
     2581 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt 
     2641 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 
     2701 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 
     2761 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact 
     2821 atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca 
     2881 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc 
     2941 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     3001 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     3061 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     3121 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     3181 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     3241 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     3301 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     3361 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     3421 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     3481 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
     3541 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     3601 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     3661 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     3721 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     3781 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     3841 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     3901 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     3961 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     4021 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     4081 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     4141 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt 
     4201 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     4261 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     4321 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     4381 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     4441 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag 
     4501 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     4561 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     4621 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     4681 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     4741 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     4801 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 
     4861 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca 
     4921 agaa 
//
