LOCUS       pCAGGS-E-hA20-Ct-ZF1        5926 bp    DNA     circular     13-JAN-2014
DEFINITION  LMBP 5928.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5928
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 13-JAN-2014
            
COMMENT     VNTDATE|511789531|
COMMENT     VNTNAME|pCAGGS-E-hA20-Ct-ZF1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hA20-Ct-ZF1" 0 5926 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "NcoI" "NotI" "PvuI" "XhoI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 793 792 1 5926 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1124 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 12 0 1959 0 4575 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3647 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 779 0 1095 0 3876 0 0 
COMMENT            "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "intron AG promoter" 15 0 0 4951 5875 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4951..5875") 
COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3203 4063 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3203..4063)") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 2261 2662 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2261..2662)") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2072 2146 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2072..2146)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2072 2146 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2072..2146") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 1966 2041 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1966..2041") 
COMMENT        #11=(CFSignal (CObList) "lac promoter" 30 0 1 1742 1824 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1742..1824)") 
COMMENT        #12=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 1216 1307 0 (CStringList) (CStringList) 1 1 1 1 "1216..1307") 
COMMENT        #13=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #14=(CFSignal (CObList) "AG promoter" 29 0 0 4582 5923 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4582..5923") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4199 4576 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4199..4576") 
COMMENT        #16=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 1308 1664 0 (CStringList) (CStringList) 1 1 1 1 "1308..1664") 
COMMENT        #17=(CFSignal (CObList) "hA20 fragment" 4 0 0 64 1122 0 (CStringList) 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 13-JAN-2014" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-hA20-Ct-ZF1'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 03/03/09 11:45" 1) 
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COMMENT                          "Last Modified: 01/13/14 12:23" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5926 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 3-MAR-2009" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                 "" 1))) "LMBP 5928") 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "   complement(1742..1824)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                            6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                            5 5 "GAANNNNTTC" "CTTNNNNAAG"))) 
COMMENT                    "Restriction/Methylation Map") 
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FEATURES             Location/Qualifiers
     intron          4951..5875
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3203..4063)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2261..2662)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2072..2146)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2072..2146
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      1966..2041
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1742..1824)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1216..1307
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        4582..5923
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4199..4576
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1308..1664
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..1122
                     /vntifkey="4"
                     /label=hA20\fragment
BASE COUNT     1263 a      1626 c      1646 g      1391 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcgatgggag aagcctatga gcccttggcg tggaaccctg aggagtccac tggggggcct 
      121 cattcggccc caccgacagc acccagccct tttctgttca gtgagaccac tgccatgaag 
      181 tgcaggagcc ccggctgccc cttcacactg aatgtgcagc acaacggatt ttgtgaacgt 
      241 tgccacaacg cccggcaact tcacgccagc cacgccccag accacacaag gcacttggat 
      301 cccgggaagt gccaagcctg cctccaggat gttaccagga catttaatgg gatctgcagt 
      361 acttgcttca aaaggactac agcagaggcc tcctccagcc tcagcaccag cctccctcct 
      421 tcctgtcacc agcgttccaa gtcagatccc tcgcggctcg tccggagccc ctccccgcat 
      481 tcttgccaca gagctggaaa cgacgcccct gctggctgcc tgtctcaagc tgcacggact 
      541 cctggggaca ggacggggac gagcaagtgc agaaaagccg gctgcgtgta ttttgggact 
      601 ccagaaaaca agggcttttg cacactgtgt ttcatcgagt acagagaaaa caaacatttt 
      661 gctgctgcct cagggaaagt cagtcccaca gcgtccaggt tccagaacac cattccgtgc 
      721 ctggggaggg aatgcggcac ccttggaagc accatgtttg aaggatactg ccagaagtgt 
      781 ttcattgaag ctcagaatca gagatttcat gaggccaaaa ggacagaaga gcaactgaga 
      841 tcgagccagc gcagagatgt gcctcgaacc acacaaagca cctcaaggcc caagtgcgcc 
      901 cgggcctcct gcaagaacat cctggcctgc cgcagcgagg agctctgcat ggagtgtcag 
      961 catcccaacc agaggatggg ccctggggcc caccggggtg agcctgcccc cgaagacccc 
     1021 cccaagcagc gttgccgggc ccccgcctgt gatcattttg gcaatgccaa gtgcaacggc 
     1081 tactgcaacg aatgctttca gttcaagcag atgtatggct aactcgaggt cgaggaattc 
     1141 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct 
     1201 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc 
     1261 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg 
     1321 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag 
     1381 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa 
     1441 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc 
     1501 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt 
     1561 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc 
     1621 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc 
     1681 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     1741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc 
     1801 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     1861 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 
     1921 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     1981 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 
     2041 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta 
     2101 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     2161 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga 
     2221 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     2281 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     2341 gtaatacggt tatccacaga atcagggata acgcaggaaa gaacatgtga gcaaaaggcc 
     2401 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     2461 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     2521 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     2581 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     2641 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     2701 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     2761 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     2821 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     2881 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     2941 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     3001 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     3061 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     3121 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     3181 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     3241 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     3301 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     3361 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     3421 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     3481 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     3541 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     3601 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     3661 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     3721 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     3781 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     3841 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     3901 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     3961 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     4021 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     4081 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     4141 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga 
     4201 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 
     4261 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 
     4321 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 
     4381 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 
     4441 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 
     4501 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 
     4561 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc 
     4621 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg 
     4681 ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga ggggcggggc 
     4741 ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct 
     4801 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga 
     4861 gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc 
     4921 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg 
     4981 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc 
     5041 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg 
     5101 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc 
     5161 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc 
     5221 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc 
     5281 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc 
     5341 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc 
     5401 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 
     5461 ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg 
     5521 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca 
     5581 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc 
     5641 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg 
     5701 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg 
     5761 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac 
     5821 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct 
     5881 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa 
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