LOCUS       pCD-HA-MAP3K7           7255 bp    DNA     circular      8-JUL-2004
DEFINITION  LMBP 5939.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5939
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 30-SEP-2010
            
            
            
COMMENT     VNTDATE|343561948|
COMMENT     VNTNAME|pCD-HA-MAP3K7|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-HA-MAP3K7" 0 7255 
COMMENT       (CDBMol 0 0 1 4448 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 121 0 "" 0 "" "" "" "" "" "" "pcDNA3.1-HAc" "" 0 0 
COMMENT             1 5510 2810 1063 1112 1063 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "XhoI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "MAP3K7-ORF" "" 0 0 1 
COMMENT             1746 1064 2809 3 1748 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "XhoI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "HindIII" "NcoI" "PvuII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7255 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1064 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 4 912 0 1340 0 2167 0 
COMMENT            2705 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 611 0 1053 0 2426 0 3786 
COMMENT            0 4521 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 1710 0 3099 0 4195 0 
COMMENT            5264 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 235 818 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "235..818") 
COMMENT        #7=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "863..879") 
COMMENT        #8=(CFSignal (CObList) "BGH polyA" 43 0 0 2852 3076 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2852..3076") 
COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 0 3122 3550 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3122..3550") 
COMMENT        #10=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3555 3836 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3555..3837") 
COMMENT        #11=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 3960 4754 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3961..4755") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4928 5002 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4929..5003") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 5316 5718 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5317..5719)") 
COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6259 7119 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6260..7120)") 
COMMENT        #15=(CFSignal (CObList) "HA epitope" 22 0 0 997 1022 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "997..1022") 
COMMENT        #16=(CFSignal (CObList) "human MAP3K7" 4 0 0 1069 2808 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1069..2808") 
COMMENT        #17=(CFSignal (CObList) "HA epitope" 22 0 0 967 993 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "967..993") 
COMMENT        #18=(CFSignal (CObList) "HA epitope" 22 0 0 1027 1053 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1027..1053") 
COMMENT        #19=(CFSignal (CObList) "SV40 ori" 33 0 0 3793 3868 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3794..3869") 
COMMENT        #20=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4928 5002 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4929..5003)") 
COMMENT        #21=(CFSignal (CObList) "lac promoter" 30 0 1 5141 5224 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5142..5225)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #22=(CFSignal (CObList) "Last modified: 30-SEP-2010" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "LMBP 5939" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT              #25=(CRefLinePar 
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COMMENT                   5 "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCD-HA-MAP3K7'" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5939." 
COMMENT                          1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: Thijs Baens. Original author: Thijs Baens" 
COMMENT                          1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 07/08/04 09:52AM" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 07/08/04 09:52AM" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7255 bp" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                    (CObjectList 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 8-JUL-2004" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #38=(CFolderPar 
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COMMENT                    (CObjectList 
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COMMENT                          "Thijs Baens" 1))) "Author") 
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COMMENT                          "Thijs Baens" 1))) "Original Author") 
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COMMENT                   0 0) 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "Last modified: 30-SEP-2010") 
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COMMENT                                 "" 1))) "LMBP 5939"))) "Annotations") 
COMMENT              #48=(CFolderPar 
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COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1069  End: 2808" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1069..2808" 1))) "human MAP3K7") 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3961..4755" 1))) 
COMMENT                                 "Tn5 neomycin resistance") 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6259  End: 7119 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6260..7120)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
COMMENT                     #62=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 967   End: 993 " 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   967..993" 1))) "HA epitope") 
COMMENT                            #67=(CFolderPar 
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COMMENT                                  (CParagraph 997 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 997   End: 1022" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   997..1022" 1))) "HA epitope") 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1027 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1027  End: 1053" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1027..1053" 1))) "HA epitope"))) 
COMMENT                          "Misc. Marker (3 total)") 
COMMENT                     #75=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 235 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 235   End: 818 " 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   235..818" 1))) 
COMMENT                                 "hCMV-IE promoter and enhancer") 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3555 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3555  End: 3836" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3555..3837" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #84=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #85=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 863 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 863   End: 879 " 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   863..879" 1))) "T7 promoter") 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5141 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5141  End: 5224 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5142..5225)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
COMMENT                     #93=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                        "Start: 3122  End: 3550" 1) 
COMMENT                                   #96=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3122..3550" 1))) "f1 ori") 
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COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
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COMMENT                                         "   3794..3869" 1))) "SV40 ori") 
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COMMENT                                          "   complement(5317..5719)" 1))) 
COMMENT                                  "pMB1 ori"))) "Replication Origin (3 total)") 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2852..3076" 1))) "BGH polyA") 
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COMMENT                                     #114=(CLinePar 
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COMMENT                                           "   4929..5003" 1))) 
COMMENT                                   "SV40 polyA early") 
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COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(4929..5003)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                     (CObjectList 
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COMMENT                                            " N1: 1064 " 1))))) "BamHI: 1 site") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
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COMMENT                                            " N1: 912 " 1) 
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COMMENT                                            " N2: 1340 " 1) 
COMMENT                                      #127=(CLinePar 
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COMMENT                                            " N3: 2167 " 1) 
COMMENT                                      #128=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 2705 " 1))))) 
COMMENT                             "HindIII: 4 sites") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                                            " N1: 1710 " 1) 
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COMMENT                                            " N2: 3099 " 1) 
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COMMENT                                            " N3: 4195 " 1) 
COMMENT                                      #134=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 5264 " 1))))) "PvuII: 4 sites") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
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FEATURES             Location/Qualifiers
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     terminator      2852..3076
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      3122..3550
                     /vntifkey="33"
                     /label=f1\ori
     promoter        3555..3836
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             3960..4754
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4928..5002
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      complement(5316..5718)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(6259..7119)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_marker     997..1022
                     /vntifkey="22"
                     /label=HA\epitope
     CDS             1069..2808
                     /vntifkey="4"
                     /label=human\MAP3K7
     misc_marker     967..993
                     /vntifkey="22"
                     /label=HA\epitope
     misc_marker     1027..1053
                     /vntifkey="22"
                     /label=HA\epitope
     rep_origin      3793..3868
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      complement(4928..5002)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        complement(5141..5224)
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     1812 a      1815 c      1801 g      1827 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttgctt gttcattttt gcagaagctc agaataaacg ctcaactttg 
      961 gccatgtacc catacgatgt tccagattac gccatgtacc catacgatgt tccagattac 
     1021 gccatgtacc catacgatgt tccagattac gccatggtac ccggatccat gtctacagcc 
     1081 tctgccgcct cctcctcctc ctcgtcttcg gccggtgaga tgatcgaagc cccttcccag 
     1141 gtcctcaact ttgaagagat cgactacaag gagatcgagg tggaagaggt tgttggaaga 
     1201 ggagcctttg gagttgtttg caaagctaag tggagagcaa aagatgttgc tattaaacaa 
     1261 atagaaagtg aatctgagag gaaagcgttt attgtagagc ttcggcagtt atcccgtgtg 
     1321 aaccatccta atattgtaaa gctttatgga gcctgcttga atccagtgtg tcttgtgatg 
     1381 gaatatgctg aagggggctc tttatataat gtgctgcatg gtgctgaacc attgccatat 
     1441 tatactgctg cccacgcaat gagttggtgt ttacagtgtt cccaaggagt ggcttatctt 
     1501 cacagcatgc aacccaaagc gctaattcac agggacctga aaccaccaaa cttactgctg 
     1561 gttgcagggg ggacagttct aaaaatttgt gattttggta cagcctgtga cattcagaca 
     1621 cacatgacca ataacaaggg gagtgctgct tggatggcac ctgaagtttt tgaaggtagt 
     1681 aattacagtg aaaaatgtga cgtcttcagc tggggtatta ttctttggga agtgataacg 
     1741 cgtcggaaac cctttgatga gattggtggc ccagctttcc gaatcatgtg ggctgttcat 
     1801 aatggtactc gaccaccact gataaaaaat ttacctaagc ccattgagag cctgatgact 
     1861 cgttgttggt ctaaagatcc ttcccagcgc ccttcaatgg aggaaattgt gaaaataatg 
     1921 actcacttga tgcggtactt tccaggagca gatgagccat tacagtatcc ttgtcagtat 
     1981 tcagatgaag gacagagcaa ctctgccacc agtacaggct cattcatgga cattgcttct 
     2041 acaaatacga gtaacaaaag tgacactaat atggagcaag ttcctgccac aaatgatact 
     2101 attaagcgct tagaatcaaa attgttgaaa aatcaggcaa agcaacagag tgaatctgga 
     2161 cgtttaagct tgggagcctc ccgtgggagc agtgtggaga gcttgccccc aacctctgag 
     2221 ggcaagagga tgagtgctga catgtctgaa atagaagcta ggatcgccgc aaccacaggc 
     2281 aacggacagc caagacgtag atccatccaa gacttgactg taactggaac agaacctggt 
     2341 caggtgagca gtaggtcatc cagtcccagt gtcagaatga ttactacctc aggaccaacc 
     2401 tcagaaaagc caactcgaag tcatccatgg acccctgatg attccacaga taccaatgga 
     2461 tcagataact ccatcccaat ggcttatctt acactggatc accaactaca gcctctagca 
     2521 ccgtgcccaa actccaaaga atctatggca gtgtttgaac agcattgtaa aatggcacaa 
     2581 gaatatatga aagttcaaac agaaattgca ttgttattac agagaaagca agaactagtt 
     2641 gcagaactgg accaggatga aaaggaccag caaaatacat ctcgcctggt acaggaacat 
     2701 aaaaagcttt tagatgaaaa caaaagcctt tctacttact accagcaatg caaaaaacaa 
     2761 ctagaggtca tcagaagtca gcagcagaaa cgacaaggca cttcatgact cgagtctaga 
     2821 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 
     2881 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 
     2941 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 
     3001 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 
     3061 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 
     3121 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 
     3181 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 
     3241 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt 
     3301 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 
     3361 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 
     3421 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 
     3481 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 
     3541 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccagctcccc 
     3601 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 
     3661 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 
     3721 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     3781 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 
     3841 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 
     3901 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca 
     3961 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 
     4021 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 
     4081 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 
     4141 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 
     4201 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 
     4261 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 
     4321 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 
     4381 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 
     4441 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 
     4501 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 
     4561 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 
     4621 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 
     4681 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 
     4741 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 
     4801 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 
     4861 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 
     4921 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     4981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     5041 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 
     5101 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 
     5161 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 
     5221 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
     5281 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     5341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     5401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     5461 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     5521 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     5581 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     5641 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 
     5701 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     5761 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     5821 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     5881 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     5941 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     6001 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     6061 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     6121 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     6181 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     6241 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     6301 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     6361 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     6421 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     6481 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     6541 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     6601 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     6661 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     6721 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     6781 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     6841 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     6901 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     6961 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     7021 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     7081 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     7141 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     7201 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc 
//
