LOCUS       pCD-F-mMK              7865 bp    DNA     circular     29-SEP-2010
DEFINITION  LMBP 5955.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5955
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 29-SEP-2010
            
COMMENT     VNTDATE|471627429|
COMMENT     VNTNAME|pCD-F-mMK|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-F-mMK" 0 7865 
COMMENT       (CDBMol 0 0 1 4432 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "HindIII" "NcoI" "PvuII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7865 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 954 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 3 912 0 2022 0 3133 0 0 
COMMENT            "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 611 0 919 0 4396 0 5131 0 
COMMENT            0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 5 1145 0 1592 0 3709 0 
COMMENT            4805 0 5874 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "FLAG epitope" 22 0 0 923 946 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "923..946") 
COMMENT        #5=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6869 7729 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(6869..7729)") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5538 5612 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "5538..5612") 
COMMENT        #7=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4570 5364 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4570..5364") 
COMMENT        #8=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4165 4446 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4165..4446") 
COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 0 3732 4160 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3732..4160") 
COMMENT        #10=(CFSignal (CObList) "BGH polyA" 43 0 0 3462 3686 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3462..3686") 
COMMENT        #11=(CFSignal (CObList) "T7_promoter" 30 0 0 863 879 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "863..879") 
COMMENT        #12=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 235 818 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "235..818") 
COMMENT        #13=(CFSignal (CObList) "mouse MALT1" 4 0 0 959 3424 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "959..3424") 
COMMENT        #14=(CFSignal (CObList) "SV40 ori" 33 0 0 4403 4478 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4403..4478") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5538 5612 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5538..5612)") 
COMMENT        #16=(CFSignal (CObList) "lac promoter" 30 0 1 5751 5834 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5751..5834)") 
COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 5926 6328 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5926..6328)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #18=(CFSignal (CObList) "Last modified: 29-SEP-2010" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "LMBP 5955" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pCD-F-mMK'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: Thijs Baens" 
COMMENT                          1) 
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COMMENT                          "Created: 01/18/08 03:37PM" 1) 
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COMMENT                          "Last Modified: 09/29/10 05:03PM" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7865 bp" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                          "Modification Date in the Original DB: 29-SEP-2010" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "Tn5 neomycin resistance") 
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COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
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COMMENT                                 "hCMV-IE promoter and enhancer") 
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COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #74=(CLinePar 
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COMMENT                                        "   863..879" 1))) "T7_promoter") 
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COMMENT                                        "   complement(5751..5834)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                   "Feature Map") 
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COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     misc_marker     923..946
                     /vntifkey="22"
                     /label=FLAG\epitope
     CDS             complement(6869..7729)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      5538..5612
                     /vntifkey="43"
                     /label=SV40\polyA\early
     CDS             4570..5364
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     promoter        4165..4446
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      3732..4160
                     /vntifkey="33"
                     /label=f1\ori
     terminator      3462..3686
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        863..879
                     /vntifkey="30"
                     /label=T7_promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             959..3424
                     /vntifkey="4"
                     /label=mouse\MALT1
     rep_origin      4403..4478
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      complement(5538..5612)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        complement(5751..5834)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(5926..6328)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     1949 a      1960 c      1968 g      1988 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ccggatccat 
      961 gtcgctgtgg gggcagccgc tgcaggcctc acctcccctg gccgtacggc agccccctac 
     1021 cgcgtcctcg ggaccttcga cctcccctcc agccggcgca accctcaacc gcctgcccga 
     1081 gccgctgctg cggaggctca gcgagagctt ggaccgcgct ccggagggcc ggggctggcg 
     1141 gcagctggcc gagctggcgg gcagtcgggg acgcctccgg ctcagtggcc tggacctgga 
     1201 acagtgttct cttaaagtat tggagccaga gggaagccca agtttgtgtt tattgaaact 
     1261 aatgggtgaa aaaggatgta cagtcacaga actgagcgac ttcctacagg ctctcgagca 
     1321 cactgaggtt cttccgcttc tcaacccccc aggattaaag atcactgtaa atccggaatc 
     1381 caaggcagtg ttggctggac agtttgtaaa actgtgctgc cgggcaactg gacatccttt 
     1441 tgtacaatat caatggttca aaatgaataa agagatccca tatggaaatt catcagaact 
     1501 tgtttttaac acagtgcatg taaaagatgc aggcttttat gtctgccggg ttaacaacag 
     1561 ttccactttt gaatttagcc agtggtcaca gctggatgtg tgtgatgtgg cagaagtaac 
     1621 agacagcttc cagggaagta tggatggcat ctcagaatcc aggttgcaaa tctgtgttga 
     1681 accaaggtcc caaaggctgg tgccaggcag catgttgttg ttacagtgtg ttgctattgg 
     1741 aagtcctatg cctcactacc agtggtttaa agatgaatca ccattaacac atgagacaaa 
     1801 aaagcactac acggttccat atgtggatat agaacatgaa ggaacctact ggtgtcatgt 
     1861 atataatgac cgagacagtc aagatagcaa gaaggcagaa gtcaccatag atgaattaaa 
     1921 caatttgggg catcctgata ataaagaaca aactggccag cctttggcaa aggacaaagt 
     1981 cgcccttttg atagggaata tgagttactg ggagcatccc aagcttaagg cgcctttggt 
     2041 ggatgtgtat gaattgacca acttactaag acaactagat ttcaaagttg tctcactgtt 
     2101 ggaccttact gaatatgaga tgtgtaacgc tgtggatgaa tttttactac ttttagacaa 
     2161 aggagtatat ggattgttat attatgcagg gcacggttat gaaaactttg gaaacagttt 
     2221 catggtccct gttgatgctc caaatccata taggtctgaa aattgcctat gcgtacaaaa 
     2281 catactgaaa ttaatgcaag aaaaggagac tggcctgaat gtgttcctgt tggatatgtg 
     2341 tcggaaaaga aatgactacg atgataccat tccaatcttg gacgcactga aagtcactgc 
     2401 caacattgtg tttggatatg ccacgtgcca aggagcagaa gcctttgaaa tccagcattc 
     2461 tgggttggca aatgggattt ttatgaaatt tttaaaagat agattattag aagacaagaa 
     2521 aattactgtg ttactggatg aagttgcaga agatatgggt aaatgtcacc ttaccaaagg 
     2581 cagacaagct ctagagattc gcagcagctt gtctgaaaag agagcactta ccgatccagt 
     2641 ccagggagca ccttgctctg cagaggctct ggtgcgcaat ctacagtggg ccaaggctca 
     2701 tgaacttcca gaaagtatgt gccttaaatt tcagtgtggc gttcacattc aattaggatt 
     2761 tgccgcagag ttctccaatg tcatgataat ctacacaagt atagtccaca aaccacctga 
     2821 gataataatg tgtgacgcct atgttactga tttccctctt gacctagata ttgatccaaa 
     2881 acatgcaaac aaggggactc ctgaagaaac tggcagctac ttagtatcaa aagaccttcc 
     2941 caagcactgc ctctacacta gactcagctc actacaaaaa ttaaaggaac atctgatctt 
     3001 cacagtctgt ctgtcctatc agtattcagg actagaagac actgtggagg agaagcagga 
     3061 ggtgaacgtg gggaagcctc ttattgccaa gctagacatg caccgaggac tgggcaggaa 
     3121 gacttgcttc caagcttgtc gtatgcctga cgagccgtac cacagctcca catctacctc 
     3181 agcgggagcg gggcatttcc attcctctca ggactcattc catgatgtgt accattcaca 
     3241 tcttggcaat gcagacagtg gtatgccacc agacaggtgt cattgcagcc ggactccaca 
     3301 cacattcatt tcaaattatc ccccccacca ctactgccag tttggtagaa gtaatgtgcc 
     3361 cgtagagaca actgatgaaa tgccattcag tttttctgac aggcttatga tttctgaaaa 
     3421 ctagctagct gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 
     3481 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 
     3541 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 
     3601 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 
     3661 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 
     3721 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     3781 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     3841 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     3901 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     3961 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     4021 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     4081 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     4141 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 
     4201 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 
     4261 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 
     4321 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 
     4381 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 
     4441 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 
     4501 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 
     4561 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 
     4621 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 
     4681 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 
     4741 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 
     4801 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 
     4861 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 
     4921 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 
     4981 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 
     5041 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 
     5101 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 
     5161 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 
     5221 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 
     5281 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 
     5341 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 
     5401 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 
     5461 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 
     5521 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 
     5581 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 
     5641 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 
     5701 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     5761 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 
     5821 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
     5881 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
     5941 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     6001 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     6061 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     6121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     6181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     6241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     6301 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     6361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     6421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     6481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     6541 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     6601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     6661 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     6721 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     6781 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     6841 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     6901 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     6961 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     7021 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     7081 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     7141 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     7201 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     7261 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     7321 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     7381 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     7441 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     7501 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     7561 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     7621 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     7681 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     7741 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     7801 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
     7861 acgtc 
//
