LOCUS       pCD-F-HLH-p20mut          6388 bp    DNA     circular      3-JUL-2006
DEFINITION  LMBP 5956.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|266935941|
COMMENT     VNTNAME|pCD-F-HLH-p20mut|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         "EcoRV" "HpaI" "PmeI" "SpeI" "BssHII" "BseAI" "Alw44I" "PinAI" "SspBI" 
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COMMENT        #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 912 0 0 "") 
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COMMENT        #8=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 33 0 4199 0 6387 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SphI" "gcatgc" 6 0 4 2185 0 2760 0 2832 0 
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COMMENT        #14=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 819 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 951 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
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COMMENT        #19=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
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COMMENT        #22=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 250 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 2 1893 0 3619 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "BseAI" "" -592361889 28290616 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "Alw44I" "" 1072917202 28290776 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "PinAI" "" 107202384 28290652 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "SspBI" "" 1073668906 28290628 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 1195 0 0 "") 
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COMMENT                          "Foreign object. Author: Peter Marynen. Original author: Peter Marynen" 
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COMMENT                          "Created: 05/17/02 12:52" 1) 
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FEATURES             Location/Qualifiers
     CDS             968..1957
                     /vntifkey="4"
                     /label=HLH-p20m
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     polyA_signal    1977..2191
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      2254..2667
                     /vntifkey="33"
                     /label=f1
     promoter        2732..3057
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     3093..3887
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    3903..4142
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      4574..5247
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(5392..6253)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     primer          complement(1975..1991)
                     /vntifkey="27"
                     /label=1503
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     modified_base   971..971
                     /vntifkey="23"
                     /label=kozak
                     /note="T replaced by G to create Kozak consensus, TCG becomes GCG,  S for A"
     protein_bind    1127..1384
                     /vntifkey="31"
                     /label=PTN
                     /note="pointed or helix loop helix domain"
     primer          1183..1204
                     /vntifkey="27"
                     /label=486
     protein_bind    1523..1924
                     /vntifkey="31"
                     /label=p20\like
     primer          complement(1462..1485)
                     /vntifkey="27"
                     /label=1706
     primer          complement(1499..1519)
                     /vntifkey="27"
                     /label=1705
     primer          1522..1539
                     /vntifkey="27"
                     /label=1744
     primer          complement(1572..1591)
                     /vntifkey="27"
                     /label=2038
     primer          complement(1572..1591)
                     /vntifkey="27"
                     /label=1745
     primer          complement(1737..1758)
                     /vntifkey="27"
                     /label=1947
     primer          1760..1779
                     /vntifkey="27"
                     /label=1344
     primer          complement(1760..1779)
                     /vntifkey="27"
                     /label=1946
     primer          complement(1783..1802)
                     /vntifkey="27"
                     /label=1678
     mutation        1892..1894
                     /vntifkey="62"
                     /label=p20-mut
                     /note="Cys of caspase p20 like mutated to Ala"
     primer          1848..1868
                     /vntifkey="27"
                     /label=1725
     primer          1931..1951
                     /vntifkey="27"
                     /label=1726
BASE COUNT     1507 a      1642 c      1612 g      1627 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc 
      961 tgccaccatg gcgctgttgg gggacccgct acaggccctg ccgccctcgg ccgcccccac 
     1021 ggggccgctg ctcgcccctc cggccggcgc gaccctcaac cgcctgcggg agccgctgct 
     1081 gcggaggctc agctacgctt cctcgacgcc acttcatgtt ccagtgcctc gagcgctcag 
     1141 gatggaggaa gactcgatcc gcctgcctgc gcacctgcgc ttgcagccaa tttactggag 
     1201 cagggatgac gtagcccagt ggctcaagtg ggctgaaaat gagttttctt taaggccaat 
     1261 tgacagcaac acgtttgaaa tgaatggcaa agctctcctg ctgctgacca aagaggactt 
     1321 tcgctatcga tctcctcatt caggtgatgt gctctatgaa ctccttcagc atattctgaa 
     1381 gcagaggaaa cctcggattc ttttttcacc attcttccac cctggaaact ctatacacac 
     1441 acagccggtt tgcactgaag atgaattaaa taatcttggt catcctgata ataaagagca 
     1501 aacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa atatgaatta 
     1561 ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga ctaacttact 
     1621 gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg agatgcgtaa 
     1681 tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat tatattatgc 
     1741 aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg ctccaaatcc 
     1801 atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc aagaaaaaga 
     1861 aactggactt aatgtgttct tattggatat ggcgcgcaaa agaaatgact acgatgatac 
     1921 cattccaatc ttggatgcac taaaagtcac cgccaatcgt ttaaacccgc tgatcagcct 
     1981 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 
     2041 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 
     2101 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 
     2161 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 
     2221 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 
     2281 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
     2341 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 
     2401 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 
     2461 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 
     2521 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 
     2581 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt 
     2641 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 
     2701 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc 
     2761 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 
     2821 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 
     2881 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     2941 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 
     3001 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 
     3061 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 
     3121 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 
     3181 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 
     3241 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 
     3301 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 
     3361 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 
     3421 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 
     3481 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 
     3541 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 
     3601 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 
     3661 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 
     3721 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 
     3781 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 
     3841 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 
     3901 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 
     3961 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 
     4021 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 
     4081 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 
     4141 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 
     4201 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 
     4261 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 
     4321 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 
     4381 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 
     4441 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 
     4501 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 
     4561 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 
     4621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 
     4681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 
     4741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 
     4801 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta 
     4861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 
     4921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 
     4981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 
     5041 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 
     5101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 
     5161 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 
     5221 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 
     5281 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 
     5341 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 
     5401 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 
     5461 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 
     5521 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 
     5581 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 
     5641 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 
     5701 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 
     5761 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 
     5821 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 
     5881 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 
     5941 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 
     6001 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 
     6061 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
     6121 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
     6181 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
     6241 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
     6301 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
     6361 atttccccga aaagtgccac ctgacgtc 
//
