LOCUS       pCD-Lck-F-MK-R149A        7922 bp    DNA     circular     17-MAY-2006
DEFINITION  LMBP 5957.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|332677856|
COMMENT     VNTNAME|pCD-Lck-F-MK-R149A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

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FEATURES             Location/Qualifiers
     misc_signal     209..863
                     /vntifkey="87"
                     /label=Pcmv
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     polyA_signal    3509..3723
                     /vntifkey="25"
                     /label=BGH\pA
     rep_origin      3786..4199
                     /vntifkey="33"
                     /label=f1
     promoter        4264..4589
                     /vntifkey="29"
                     /label=SV40\prom\ori
     misc_recomb     4625..5419
                     /vntifkey="86"
                     /label=Neomycin
     polyA_signal    5435..5674
                     /vntifkey="25"
                     /label=SV40\pA
     rep_origin      6106..6779
                     /vntifkey="33"
                     /label=Col\E1
     misc_marker     complement(6924..7785)
                     /vntifkey="22"
                     /label=AmpR
     primer          863..882
                     /vntifkey="27"
                     /label=2506
                     /note="T7"
     primer          complement(3507..3523)
                     /vntifkey="27"
                     /label=1503
     protein_bind    914..949
                     /vntifkey="31"
                     /label=LRS
                     /note="lipid raft signalsequence"
     misc_feature    965..991
                     /vntifkey="21"
                     /label=flag\epitope
     protein_bind    1688..1894
                     /vntifkey="31"
                     /label=IgL2
     protein_bind    2972..3103
                     /vntifkey="31"
                     /label=VDJ4
     modified_base   2500..2500
                     /vntifkey="23"
                     /label=stability\creator
                     /note="T replaces A as GCAGAAG recombines with the same heptanucleotide present in API2"
     mutation        2962..2962
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation, G replaces A"
     protein_bind    2042..2434
                     /vntifkey="31"
                     /label=casp\like
     primer          complement(1640..1659)
                     /vntifkey="27"
                     /label=2010
     primer          complement(1665..1684)
                     /vntifkey="27"
                     /label=1943
     primer          complement(1758..1777)
                     /vntifkey="27"
                     /label=1987
     primer          complement(1809..1832)
                     /vntifkey="27"
                     /label=1988
     primer          1899..1916
                     /vntifkey="27"
                     /label=1743
     primer          1953..1970
                     /vntifkey="27"
                     /label=1707
     primer          complement(1972..1995)
                     /vntifkey="27"
                     /label=1706
     primer          complement(2009..2029)
                     /vntifkey="27"
                     /label=1705
     primer          2032..2049
                     /vntifkey="27"
                     /label=1744
     primer          complement(2082..2101)
                     /vntifkey="27"
                     /label=2038
     primer          complement(2082..2101)
                     /vntifkey="27"
                     /label=1745
     primer          complement(2247..2268)
                     /vntifkey="27"
                     /label=1947
     primer          2270..2289
                     /vntifkey="27"
                     /label=1344
     primer          complement(2270..2289)
                     /vntifkey="27"
                     /label=1946
     primer          complement(2293..2312)
                     /vntifkey="27"
                     /label=1678
     primer          2358..2378
                     /vntifkey="27"
                     /label=1725
     primer          2441..2461
                     /vntifkey="27"
                     /label=1726
     primer          2501..2516
                     /vntifkey="27"
                     /label=1727
                     /note="5 extra bases at the 5' end, one off which is not complementary"
     primer          2528..2548
                     /vntifkey="27"
                     /label=1728
     primer          complement(2597..2615)
                     /vntifkey="27"
                     /label=1343
     primer          complement(2733..2752)
                     /vntifkey="27"
                     /label=2012
     primer          2822..2843
                     /vntifkey="27"
                     /label=3284
     primer          complement(3337..3354)
                     /vntifkey="27"
                     /label=3285
     primer          3265..3284
                     /vntifkey="27"
                     /label=3429
     primer          complement(2893..2917)
                     /vntifkey="27"
                     /label=3739
     primer          complement(3223..3245)
                     /vntifkey="27"
                     /label=3740
     primer          complement(3382..3405)
                     /vntifkey="27"
                     /label=3794
     primer          3277..3296
                     /vntifkey="27"
                     /label=3862
                     /note="(RT-)PCR primer for API2-MLT transgenesis"
     primer          3203..3223
                     /vntifkey="27"
                     /label=3824
     primer          complement(3464..3484)
                     /vntifkey="27"
                     /label=3825
     primer          complement(3377..3397)
                     /vntifkey="27"
                     /label=3826
     primer          3378..3396
                     /vntifkey="27"
                     /label=si-MLT1
     primer          2724..2744
                     /vntifkey="27"
                     /label=4265
     primer          complement(2769..2795)
                     /vntifkey="27"
                     /label=4266
     primer          2822..2841
                     /vntifkey="27"
                     /label=4342
     primer          1666..1683
                     /vntifkey="27"
                     /label=si-MLT2
     primer          1408..1427
                     /vntifkey="27"
                     /label=1673
     primer          complement(1486..1509)
                     /vntifkey="27"
                     /label=1677
     primer          1517..1534
                     /vntifkey="27"
                     /label=1742
     primer          1565..1584
                     /vntifkey="27"
                     /label=1985
     primer          complement(1602..1621)
                     /vntifkey="27"
                     /label=2011
     primer          1605..1624
                     /vntifkey="27"
                     /label=1986
     modified_base   1016..1016
                     /vntifkey="23"
                     /label=kozak
                     /note="T replaced by G to create Kozak consensus, TCG becomes GCG,  S for A"
     protein_bind    1154..1378
                     /vntifkey="31"
                     /label=DD
                     /note="Death Domain"
     primer          complement(1174..1191)
                     /vntifkey="27"
                     /label=1955
     primer          complement(1216..1235)
                     /vntifkey="27"
                     /label=3228
     primer          1222..1241
                     /vntifkey="27"
                     /label=1963
     primer          1296..1318
                     /vntifkey="27"
                     /label=1953
                     /note="contains BamHI tail"
     primer          1334..1353
                     /vntifkey="27"
                     /label=1965
     primer          complement(1364..1383)
                     /vntifkey="27"
                     /label=1909
     CDS             1013..3484
                     /vntifkey="4"
                     /label=MLT-R149A
BASE COUNT     1979 a      1915 c      1980 g      2048 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatcctgcca ccatggcgct 
     1021 gttgggggac ccgctacagg ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc 
     1081 ccctccggcc ggcgcgaccc tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga 
     1141 gctcctggat caggcgcccg agggccgggg ctggaggaga ctggcggagc tggcggggag 
     1201 tcgcgggcgc ctccgcctca gttgcctaga cctggagcag tgttctctta aggtactgga 
     1261 gcctgaagga agccccagcc tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt 
     1321 cacagaattg agtgatttcc tgcaggctat ggaacacact gaagttcttc agcttctcag 
     1381 ccccccagga ataaagatta ctgttaaccc agagtcaaag gcagtcttgg ctggacagtt 
     1441 tgtgaaactg tgttgcgcag caactggaca tccttttgtt caatatcagt ggttcaaaat 
     1501 gaataaagag attccaaatg gaaatacatc agagcttatt tttaatgcag tgcatgtaaa 
     1561 agatgcaggc ttttatgtct gtcgagttaa taacaatttc acctttgaat tcagccagtg 
     1621 gtcacagctg gatgtttgcg acatcccaga gagcttccag agaagtgttg atggcgtctc 
     1681 tgaatccaag ttgcaaatct gtgttgaacc aacttcccaa aagctgatgc caggcagcac 
     1741 attggtttta cagtgtgttg ctgttggaag ccctattcct cactaccagt ggttcaaaaa 
     1801 tgaattacca ttaacacatg agaccaaaaa gctatacatg gtgccttatg tggatttgga 
     1861 acaccaagga acctactggt gtcatgtata taatgatcga gacagtcaag atagcaagaa 
     1921 ggtagaaatc atcataggaa gaacagatga ggcagtggag tgcactgaag atgaattaaa 
     1981 taatcttggt catcctgata ataaagagca aacaactgac cagcctttgg cgaaggacaa 
     2041 ggttgccctt ttgataggaa atatgaatta ccgggagcac cccaagctca aagctccttt 
     2101 ggtggatgtg tacgaattga ctaacttact gagacagctg gacttcaaag tggtttcact 
     2161 gttggatctt actgaatatg agatgcgtaa tgctgtggat gagtttttac tccttttaga 
     2221 caagggagta tatgggttat tatattatgc aggacatggt tatgaaaatt ttgggaacag 
     2281 cttcatggtc cccgttgatg ctccaaatcc atataggtct gaaaattgtc tgtgtgtaca 
     2341 aaatatactg aaattgatgc aagaaaaaga aactggactt aatgtgttct tattggatat 
     2401 gtgtaggaaa agaaatgact acgatgatac cattccaatc ttggatgcac taaaagtcac 
     2461 cgccaatatt gtgtttggat atgccacgtg tcaaggagct gaagcttttg aaatccagca 
     2521 ttctggattg gcaaatggaa tctttatgaa atttttaaaa gacagattat tagaagataa 
     2581 gaaaatcact gtgttactgg atgaagttgc agaagatatg ggtaagtgtc accttaccaa 
     2641 aggcaaacag gctctagaga ttcgaagtag tttatctgag aagagagcac ttactgatcc 
     2701 aatacaggga acagaatatt ctgctgaatc tcttgtgcgg aatctacagt gggccaaggc 
     2761 tcatgaactt ccagaaagta tgtgtcttaa gtttgactgt ggtgttcaga ttcaattagg 
     2821 atttgcagct gagttttcca atgtcatgat catctataca agtatagttt acaaaccacc 
     2881 ggagataata atgtgtgatg cctacgttac tgattttcca cttgatctag atattgatcc 
     2941 aaaagatgca aataaaggca cgcctgaaga aactggcagc tacttggtat caaaggatct 
     3001 tcccaagcat tgcctctata ccagactcag ttcactgcaa aaattaaagg aacatctagt 
     3061 cttcacagta tgtttatcat atcagtactc aggattggaa gatactgtag aggacaagca 
     3121 ggaagtgaat gttgggaaac ctctcattgc taaattagac atgcatcgag gtttgggaag 
     3181 gaagacttgc tttcaaactt gtcttatgtc taatggtcct taccagagtt ctgcagccac 
     3241 ctcaggagga gcagggcatt atcactcatt gcaagaccca ttccatggtg tttaccattc 
     3301 acatcctggt aatccaagta atgttacacc agcagatagc tgtcattgca gccggactcc 
     3361 agatgcattt atttcaagtt tcgctcacca tgcttcatgt cattttagta gaagtaatgt 
     3421 gccagtagag acaactgatg aaataccatt tagtttctct gacaggctca gaatttctga 
     3481 aaaagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 
     3541 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 
     3601 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 
     3661 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 
     3721 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 
     3781 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 
     3841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 
     3901 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg 
     3961 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 
     4021 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 
     4081 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 
     4141 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 
     4201 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 
     4261 tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 
     4321 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 
     4381 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     4441 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 
     4501 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 
     4561 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt 
     4621 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 
     4681 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 
     4741 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 
     4801 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 
     4861 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 
     4921 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 
     4981 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 
     5041 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 
     5101 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 
     5161 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 
     5221 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 
     5281 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 
     5341 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 
     5401 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc 
     5461 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 
     5521 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 
     5581 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     5641 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     5701 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 
     5761 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 
     5821 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 
     5881 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 
     5941 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 
     6001 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     6061 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     6121 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     6181 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     6241 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     6301 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     6361 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     6421 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     6481 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     6541 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     6601 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     6661 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     6721 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     6781 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     6841 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     6901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     6961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     7021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     7081 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     7141 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     7201 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     7261 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     7321 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     7381 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     7441 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     7501 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     7561 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     7621 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     7681 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     7741 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     7801 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     7861 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
     7921 tc 
//
