LOCUS       pCD-F-MK-p76            7422 bp    DNA     circular      3-JUL-2006
DEFINITION  LMBP 5959.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|331117989|
COMMENT     VNTNAME|pCD-F-MK-p76|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

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FEATURES             Location/Qualifiers
     CDS             962..2986
                     /vntifkey="4"
                     /label=MK-p76-Nter
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     polyA_signal    3011..3225
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      3288..3701
                     /vntifkey="33"
                     /label=f1
     promoter        3766..4091
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     4127..4921
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    4937..5176
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      5608..6281
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(6426..7287)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     primer          complement(3009..3025)
                     /vntifkey="27"
                     /label=1503
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     protein_bind    1190..1396
                     /vntifkey="31"
                     /label=IgL2
     protein_bind    2474..2605
                     /vntifkey="31"
                     /label=VDJ4
     modified_base   2002..2002
                     /vntifkey="23"
                     /label=stability\creator
                     /note="T replaces A as GCAGAAG recombines with the same heptanucleotide present in API2"
     mutation        2464..2464
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation, G replaces A"
     protein_bind    1544..1936
                     /vntifkey="31"
                     /label=casp\like
     primer          complement(1142..1161)
                     /vntifkey="27"
                     /label=2010
     primer          complement(1167..1186)
                     /vntifkey="27"
                     /label=1943
     primer          complement(1260..1279)
                     /vntifkey="27"
                     /label=1987
     primer          complement(1311..1334)
                     /vntifkey="27"
                     /label=1988
     primer          1401..1418
                     /vntifkey="27"
                     /label=1743
     primer          1455..1472
                     /vntifkey="27"
                     /label=1707
     primer          complement(1474..1497)
                     /vntifkey="27"
                     /label=1706
     primer          complement(1511..1531)
                     /vntifkey="27"
                     /label=1705
     primer          1534..1551
                     /vntifkey="27"
                     /label=1744
     primer          complement(1584..1603)
                     /vntifkey="27"
                     /label=2038
     primer          complement(1584..1603)
                     /vntifkey="27"
                     /label=1745
     primer          complement(1749..1770)
                     /vntifkey="27"
                     /label=1947
     primer          1772..1791
                     /vntifkey="27"
                     /label=1344
     primer          complement(1772..1791)
                     /vntifkey="27"
                     /label=1946
     primer          complement(1795..1814)
                     /vntifkey="27"
                     /label=1678
     primer          1860..1880
                     /vntifkey="27"
                     /label=1725
     primer          1943..1963
                     /vntifkey="27"
                     /label=1726
     primer          2003..2018
                     /vntifkey="27"
                     /label=1727
                     /note="5 extra bases at the 5' end, one off which is not complementary"
     primer          2030..2050
                     /vntifkey="27"
                     /label=1728
     primer          complement(2099..2117)
                     /vntifkey="27"
                     /label=1343
     primer          complement(2235..2254)
                     /vntifkey="27"
                     /label=2012
     primer          2324..2345
                     /vntifkey="27"
                     /label=3284
     primer          complement(2839..2856)
                     /vntifkey="27"
                     /label=3285
     primer          2767..2786
                     /vntifkey="27"
                     /label=3429
     primer          complement(2395..2419)
                     /vntifkey="27"
                     /label=3739
     primer          complement(2725..2747)
                     /vntifkey="27"
                     /label=3740
     primer          complement(2884..2907)
                     /vntifkey="27"
                     /label=3794
     primer          2779..2798
                     /vntifkey="27"
                     /label=3862
                     /note="(RT-)PCR primer for API2-MLT transgenesis"
     primer          2705..2725
                     /vntifkey="27"
                     /label=3824
     primer          complement(2966..2986)
                     /vntifkey="27"
                     /label=3825
     primer          complement(2879..2899)
                     /vntifkey="27"
                     /label=3826
     primer          2880..2898
                     /vntifkey="27"
                     /label=si-MLT1
     primer          2226..2246
                     /vntifkey="27"
                     /label=4265
     primer          complement(2271..2297)
                     /vntifkey="27"
                     /label=4266
     primer          2324..2343
                     /vntifkey="27"
                     /label=4342
     primer          1168..1185
                     /vntifkey="27"
                     /label=si-MLT2
     protein_bind    2924..2941
                     /vntifkey="31"
                     /label=TRAF6
     primer          2088..2106
                     /vntifkey="27"
                     /label=siRNA
                     /note="lijun sun, molec cell 14 (2004)"
     primer          complement(988..1011)
                     /vntifkey="27"
                     /label=1677
     primer          1019..1036
                     /vntifkey="27"
                     /label=1742
     primer          1067..1086
                     /vntifkey="27"
                     /label=1985
     primer          complement(1051..1071)
                     /vntifkey="27"
                     /label=4340
     primer          complement(1104..1123)
                     /vntifkey="27"
                     /label=2011
BASE COUNT     1886 a      1766 c      1817 g      1953 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc 
      961 tgcaactgga catccttttg ttcaatatca gtggttcaaa atgaataaag agattccaaa 
     1021 tggaaataca tcagagctta tttttaatgc agtgcatgta aaagatgcag gcttttatgt 
     1081 ctgtcgagtt aataacaatt tcacctttga attcagccag tggtcacagc tggatgtttg 
     1141 cgacatccca gagagcttcc agagaagtgt tgatggcgtc tctgaatcca agttgcaaat 
     1201 ctgtgttgaa ccaacttccc aaaagctgat gccaggcagc acattggttt tacagtgtgt 
     1261 tgctgttgga agccctattc ctcactacca gtggttcaaa aatgaattac cattaacaca 
     1321 tgagaccaaa aagctataca tggtgcctta tgtggatttg gaacaccaag gaacctactg 
     1381 gtgtcatgta tataatgatc gagacagtca agatagcaag aaggtagaaa tcatcatagg 
     1441 aagaacagat gaggcagtgg agtgcactga agatgaatta aataatcttg gtcatcctga 
     1501 taataaagag caaacaactg accagccttt ggcgaaggac aaggttgccc ttttgatagg 
     1561 aaatatgaat taccgggagc accccaagct caaagctcct ttggtggatg tgtacgaatt 
     1621 gactaactta ctgagacagc tggacttcaa agtggtttca ctgttggatc ttactgaata 
     1681 tgagatgcgt aatgctgtgg atgagttttt actcctttta gacaagggag tatatgggtt 
     1741 attatattat gcaggacatg gttatgaaaa ttttgggaac agcttcatgg tccccgttga 
     1801 tgctccaaat ccatataggt ctgaaaattg tctgtgtgta caaaatatac tgaaattgat 
     1861 gcaagaaaaa gaaactggac ttaatgtgtt cttattggat atgtgtagga aaagaaatga 
     1921 ctacgatgat accattccaa tcttggatgc actaaaagtc accgccaata ttgtgtttgg 
     1981 atatgccacg tgtcaaggag ctgaagcttt tgaaatccag cattctggat tggcaaatgg 
     2041 aatctttatg aaatttttaa aagacagatt attagaagat aagaaaatca ctgtgttact 
     2101 ggatgaagtt gcagaagata tgggtaagtg tcaccttacc aaaggcaaac aggctctaga 
     2161 gattcgaagt agtttatctg agaagagagc acttactgat ccaatacagg gaacagaata 
     2221 ttctgctgaa tctcttgtgc ggaatctaca gtgggccaag gctcatgaac ttccagaaag 
     2281 tatgtgtctt aagtttgact gtggtgttca gattcaatta ggatttgcag ctgagttttc 
     2341 caatgtcatg atcatctata caagtatagt ttacaaacca ccggagataa taatgtgtga 
     2401 tgcctacgtt actgattttc cacttgatct agatattgat ccaaaagatg caaataaagg 
     2461 cacgcctgaa gaaactggca gctacttggt atcaaaggat cttcccaagc attgcctcta 
     2521 taccagactc agttcactgc aaaaattaaa ggaacatcta gtcttcacag tatgtttatc 
     2581 atatcagtac tcaggattgg aagatactgt agaggacaag caggaagtga atgttgggaa 
     2641 acctctcatt gctaaattag acatgcatcg aggtttggga aggaagactt gctttcaaac 
     2701 ttgtcttatg tctaatggtc cttaccagag ttctgcagcc acctcaggag gagcagggca 
     2761 ttatcactca ttgcaagacc cattccatgg tgtttaccat tcacatcctg gtaatccaag 
     2821 taatgttaca ccagcagata gctgtcattg cagccggact ccagatgcat ttatttcaag 
     2881 tttcgctcac catgcttcat gtcattttag tagaagtaat gtgccagtag agacaactga 
     2941 tgaaatacca tttagtttct ctgacaggct cagaatttct gaaaaagggc ccgtttaaac 
     3001 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 
     3061 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 
     3121 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 
     3181 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 
     3241 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 
     3301 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     3361 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     3421 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca 
     3481 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
     3541 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     3601 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg 
     3661 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 
     3721 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag 
     3781 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     3841 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     3901 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     3961 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 
     4021 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 
     4081 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 
     4141 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 
     4201 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 
     4261 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 
     4321 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 
     4381 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 
     4441 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 
     4501 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 
     4561 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 
     4621 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 
     4681 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 
     4741 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 
     4801 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 
     4861 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 
     4921 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 
     4981 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 
     5041 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg 
     5101 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 
     5161 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 
     5221 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 
     5281 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
     5341 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 
     5401 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 
     5461 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
     5521 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
     5581 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
     5641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
     5701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
     5761 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
     5821 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 
     5881 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
     5941 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
     6001 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
     6061 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 
     6121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
     6181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
     6241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
     6301 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 
     6361 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 
     6421 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 
     6481 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 
     6541 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 
     6601 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 
     6661 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 
     6721 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 
     6781 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 
     6841 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 
     6901 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 
     6961 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 
     7021 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 
     7081 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 
     7141 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 
     7201 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 
     7261 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 
     7321 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 
     7381 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc 
//
