LOCUS       pCD-F-MALT1-C           6870 bp    DNA     circular      3-JUL-2006
DEFINITION  LMBP 5962.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|345918725|
COMMENT     VNTNAME|pCD-F-MALT1-C|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (CDBMol 0 0 1 5586 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
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COMMENT        #4=(CFSignal (CObList) "T7" 30 0 0 863 882 0 (CStringList) (CStringList) 
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COMMENT        #5=(CFSignal (CObList) "BGH polyA" 25 0 0 2459 2673 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2459..2673") 
COMMENT        #6=(CFSignal (CObList) "f1" 33 0 0 2736 3149 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "SV40" 29 0 0 3214 3539 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "NeoR" 86 0 0 3575 4369 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "ColE1" 33 0 0 5056 5729 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "VDJ4" 31 0 0 1922 2053 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "stability creator" 23 0 0 1450 1450 0 
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COMMENT        #17=(CFSignal (CObList) "PCR artefact" 62 0 0 1912 1912 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "1725" 27 0 0 1308 1328 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "1726" 27 0 0 1391 1411 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "1727" 27 0 0 1451 1466 0 (CStringList) 
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COMMENT                          "Foreign object. Author: Peter Marynen. Original author: Peter Marynen" 
COMMENT                          1) 
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COMMENT                          "Created: 08/03/04 04:32" 1) 
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COMMENT                          "Last Modified: 07/03/06 06:59" 1) 
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FEATURES             Location/Qualifiers
     CDS             962..2434
                     /vntifkey="4"
                     /label=MALT1-C
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     polyA_signal    2459..2673
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      2736..3149
                     /vntifkey="33"
                     /label=f1
     promoter        3214..3539
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     3575..4369
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    4385..4624
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      5056..5729
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(5874..6735)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     primer          complement(2457..2473)
                     /vntifkey="27"
                     /label=1503
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     protein_bind    1922..2053
                     /vntifkey="31"
                     /label=VDJ4
     modified_base   1450..1450
                     /vntifkey="23"
                     /label=stability\creator
                     /note="T replaces A as GCAGAAG recombines with the same heptanucleotide present in API2"
     mutation        1912..1912
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation, G replaces A"
     primer          1308..1328
                     /vntifkey="27"
                     /label=1725
     primer          1391..1411
                     /vntifkey="27"
                     /label=1726
     primer          1451..1466
                     /vntifkey="27"
                     /label=1727
                     /note="5 extra bases at the 5' end, one off which is not complementary"
     primer          1478..1498
                     /vntifkey="27"
                     /label=1728
     primer          complement(1547..1565)
                     /vntifkey="27"
                     /label=1343
     primer          complement(1683..1702)
                     /vntifkey="27"
                     /label=2012
     primer          1772..1793
                     /vntifkey="27"
                     /label=3284
     primer          complement(2287..2304)
                     /vntifkey="27"
                     /label=3285
     primer          2215..2234
                     /vntifkey="27"
                     /label=3429
     primer          complement(1843..1867)
                     /vntifkey="27"
                     /label=3739
     primer          complement(2173..2195)
                     /vntifkey="27"
                     /label=3740
     primer          complement(2332..2355)
                     /vntifkey="27"
                     /label=3794
     primer          2227..2246
                     /vntifkey="27"
                     /label=3862
                     /note="(RT-)PCR primer for API2-MLT transgenesis"
     primer          2153..2173
                     /vntifkey="27"
                     /label=3824
     primer          complement(2414..2434)
                     /vntifkey="27"
                     /label=3825
     primer          complement(2327..2347)
                     /vntifkey="27"
                     /label=3826
     primer          2328..2346
                     /vntifkey="27"
                     /label=si-MLT1
     primer          1674..1694
                     /vntifkey="27"
                     /label=4265
     primer          complement(1719..1745)
                     /vntifkey="27"
                     /label=4266
     primer          1772..1791
                     /vntifkey="27"
                     /label=4342
     protein_bind    2372..2389
                     /vntifkey="31"
                     /label=TRAF6
     primer          1536..1554
                     /vntifkey="27"
                     /label=siRNA
                     /note="lijun sun, molec cell 14 (2004)"
     primer          982..999
                     /vntifkey="27"
                     /label=1744
     primer          complement(1032..1051)
                     /vntifkey="27"
                     /label=2038
     primer          complement(1032..1051)
                     /vntifkey="27"
                     /label=1745
     primer          complement(1197..1218)
                     /vntifkey="27"
                     /label=1947
     primer          1220..1239
                     /vntifkey="27"
                     /label=1344
     primer          complement(1220..1239)
                     /vntifkey="27"
                     /label=1946
     primer          complement(1243..1262)
                     /vntifkey="27"
                     /label=1678
     protein_bind    992..1384
                     /vntifkey="31"
                     /label=casp\like
BASE COUNT     1705 a      1666 c      1696 g      1803 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc 
      961 tacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa atatgaatta 
     1021 ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga ctaacttact 
     1081 gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg agatgcgtaa 
     1141 tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat tatattatgc 
     1201 aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg ctccaaatcc 
     1261 atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc aagaaaaaga 
     1321 aactggactt aatgtgttct tattggatat gtgtaggaaa agaaatgact acgatgatac 
     1381 cattccaatc ttggatgcac taaaagtcac cgccaatatt gtgtttggat atgccacgtg 
     1441 tcaaggagct gaagcttttg aaatccagca ttctggattg gcaaatggaa tctttatgaa 
     1501 atttttaaaa gacagattat tagaagataa gaaaatcact gtgttactgg atgaagttgc 
     1561 agaagatatg ggtaagtgtc accttaccaa aggcaaacag gctctagaga ttcgaagtag 
     1621 tttatctgag aagagagcac ttactgatcc aatacaggga acagaatatt ctgctgaatc 
     1681 tcttgtgcgg aatctacagt gggccaaggc tcatgaactt ccagaaagta tgtgtcttaa 
     1741 gtttgactgt ggtgttcaga ttcaattagg atttgcagct gagttttcca atgtcatgat 
     1801 catctataca agtatagttt acaaaccacc ggagataata atgtgtgatg cctacgttac 
     1861 tgattttcca cttgatctag atattgatcc aaaagatgca aataaaggca cgcctgaaga 
     1921 aactggcagc tacttggtat caaaggatct tcccaagcat tgcctctata ccagactcag 
     1981 ttcactgcaa aaattaaagg aacatctagt cttcacagta tgtttatcat atcagtactc 
     2041 aggattggaa gatactgtag aggacaagca ggaagtgaat gttgggaaac ctctcattgc 
     2101 taaattagac atgcatcgag gtttgggaag gaagacttgc tttcaaactt gtcttatgtc 
     2161 taatggtcct taccagagtt ctgcagccac ctcaggagga gcagggcatt atcactcatt 
     2221 gcaagaccca ttccatggtg tttaccattc acatcctggt aatccaagta atgttacacc 
     2281 agcagatagc tgtcattgca gccggactcc agatgcattt atttcaagtt tcgctcacca 
     2341 tgcttcatgt cattttagta gaagtaatgt gccagtagag acaactgatg aaataccatt 
     2401 tagtttctct gacaggctca gaatttctga aaaagggccc gtttaaaccc gctgatcagc 
     2461 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 
     2521 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 
     2581 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 
     2641 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 
     2701 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 
     2761 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 
     2821 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 
     2881 tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 
     2941 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 
     3001 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 
     3061 actcaaccct atctcggtct attcttttga tttataaggg attttgggga tttcggccta 
     3121 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg 
     3181 tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat 
     3241 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 
     3301 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 
     3361 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 
     3421 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 
     3481 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 
     3541 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 
     3601 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 
     3661 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 
     3721 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 
     3781 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 
     3841 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 
     3901 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 
     3961 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 
     4021 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 
     4081 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 
     4141 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 
     4201 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 
     4261 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 
     4321 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 
     4381 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 
     4441 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 
     4501 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 
     4561 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     4621 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 
     4681 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 
     4741 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 
     4801 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 
     4861 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 
     4921 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     4981 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
     5041 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
     5101 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     5161 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     5221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     5281 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 
     5341 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     5401 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     5461 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     5521 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 
     5581 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     5641 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     5701 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
     5761 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
     5821 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
     5881 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
     5941 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
     6001 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
     6061 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
     6121 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
     6181 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
     6241 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
     6301 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
     6361 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
     6421 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
     6481 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
     6541 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
     6601 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
     6661 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
     6721 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 
     6781 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 
     6841 acatttcccc gaaaagtgcc acctgacgtc 
//
