LOCUS       pCD-F-bio-MK-N149        5898 bp    DNA     circular      8-MAY-2007
DEFINITION  LMBP 5967.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|409675279|
COMMENT     VNTNAME|pCD-F-bio-MK-N149|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-F-bio-MK-N149" 0 5898 
COMMENT       (CDBMol 0 0 1 4432 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
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COMMENT             (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
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COMMENT         "SacI" "SalI" "XbaI" "XhoI") (CStringList "atg") 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #2=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 1468 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1001 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 912 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 951 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 819 0 1144 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 33 0 3709 0 5897 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "")) 
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COMMENT        #15=(CFSignal (CObList) "f1" 33 0 0 1764 2177 0 (CStringList) 
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COMMENT        #21=(CFSignal (CObList) "ColE1" 33 0 0 4084 4757 0 (CStringList) 
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COMMENT        #22=(CFSignal (CObList) "SV40 polyA" 25 0 0 3413 3652 0 (CStringList) 
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COMMENT        #23=(CFSignal (CObList) "NeoR" 86 0 0 2603 3397 0 (CStringList) 
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COMMENT        #24=(CFSignal (CObList) "T7" 30 0 0 863 882 0 (CStringList) 
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COMMENT                          "DNA 'pCD-F-bio-MK-N149'" 1) 
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COMMENT                          "Foreign object. Author: Peter Marynen. Original author: Peter Marynen" 
COMMENT                          1) 
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COMMENT                          "Created: 05/05/06 02:41" 1) 
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COMMENT                          "Last Modified: 05/08/07 05:32" 1) 
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FEATURES             Location/Qualifiers
     promoter        2242..2567
                     /vntifkey="29"
                     /label=SV40
     primer          complement(1485..1501)
                     /vntifkey="27"
                     /label=1503
     rep_origin      1764..2177
                     /vntifkey="33"
                     /label=f1
     polyA_signal    1487..1701
                     /vntifkey="25"
                     /label=BGH\polyA
     primer          947..982
                     /vntifkey="27"
                     /label=5925
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     misc_marker     complement(4902..5763)
                     /vntifkey="22"
                     /label=AmpR
     rep_origin      4084..4757
                     /vntifkey="33"
                     /label=ColE1
     polyA_signal    3413..3652
                     /vntifkey="25"
                     /label=SV40\polyA
     misc_recomb     2603..3397
                     /vntifkey="86"
                     /label=NeoR
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     misc_feature    953..997
                     /vntifkey="21"
                     /label=bio
                     /note="synthetic biotinylation sequence"
     primer          complement(1439..1458)
                     /vntifkey="27"
                     /label=1762
     primer          1408..1427
                     /vntifkey="27"
                     /label=1673
     primer          complement(1364..1383)
                     /vntifkey="27"
                     /label=1909
     primer          1334..1353
                     /vntifkey="27"
                     /label=1965
     primer          1296..1318
                     /vntifkey="27"
                     /label=1953
                     /note="contains BamHI tail"
     primer          1222..1241
                     /vntifkey="27"
                     /label=1963
     primer          complement(1216..1235)
                     /vntifkey="27"
                     /label=3228
     primer          complement(1174..1191)
                     /vntifkey="27"
                     /label=1955
     protein_bind    1154..1378
                     /vntifkey="31"
                     /label=DD
                     /note="Death Domain"
     modified_base   1016..1016
                     /vntifkey="23"
                     /label=kozak
                     /note="T replaced by G to create Kozak consensus, TCG becomes GCG,  S for A"
     CDS             1013..1459
                     /vntifkey="4"
                     /label=MLT-R149-Nter
BASE COUNT     1347 a      1547 c      1541 g      1463 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac caggcctgaa 
      961 cgacatcttc gaggcccaga agatcgagtg gcacgaggag gatcctgcca ccatggcgct 
     1021 gttgggggac ccgctacagg ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc 
     1081 ccctccggcc ggcgcgaccc tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga 
     1141 gctcctggat caggcgcccg agggccgggg ctggaggaga ctggcggagc tggcggggag 
     1201 tcgcgggcgc ctccgcctca gttgcctaga cctggagcag tgttctctta aggtactgga 
     1261 gcctgaagga agccccagcc tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt 
     1321 cacagaattg agtgatttcc tgcaggctat ggaacacact gaagttcttc agcttctcag 
     1381 ccccccagga ataaagatta ctgtaaaccc agagtcaaag gcagtcttgg ctggacagtt 
     1441 tgtgaaactg tgttgccggt gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc 
     1501 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 
     1561 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 
     1621 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 
     1681 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 
     1741 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     1801 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     1861 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     1921 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     1981 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     2041 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     2101 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa 
     2161 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 
     2221 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta 
     2281 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     2341 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     2401 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     2461 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     2521 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 
     2581 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     2641 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     2701 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     2761 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 
     2821 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     2881 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     2941 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     3001 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     3061 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     3121 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     3181 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     3241 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     3301 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     3361 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 
     3421 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 
     3481 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 
     3541 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 
     3601 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     3661 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 
     3721 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 
     3781 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 
     3841 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     3901 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     3961 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     4021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 
     4081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     4141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     4201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     4261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     4321 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     4381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     4441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     4501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     4561 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 
     4621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     4681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     4741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     4801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     4861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     4921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     4981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     5041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     5101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     5161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     5221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     5281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     5341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     5401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     5461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     5521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     5581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     5641 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     5701 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     5761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     5821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     5881 aaagtgccac ctgacgtc 
//
