LOCUS       pCD-F-AtSerpin-IKLA        6564 bp    DNA     circular     20-MAY-2008
DEFINITION  LMBP 5991.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|482843537|
COMMENT     VNTDBDATE|482843537|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCD-F-AtSerpin-IKLA|
COMMENT     VNTAUTHORNAME|Jens Staal|
COMMENT     VNTAUTHOREML|jens.staal@dmbr.ugent.be|
COMMENT     VNTAUTHORWWW|www.dmbr.ugent.be|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     polyA_signal    2153..2367
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      2430..2843
                     /vntifkey="33"
                     /label=f1
     promoter        2908..3233
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     3269..4063
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    4079..4318
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      4750..5423
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(5568..6429)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     primer          complement(2151..2167)
                     /vntifkey="27"
                     /label=1503
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     CDS             953..2128
                     /vntifkey="4"
                     /label=AtSerpin1-IKLA
     misc_feature    1994..2005
                     /vntifkey="21"
                     /label=IKLA
BASE COUNT     1583 a      1655 c      1629 g      1697 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctatggacgt 
      961 gcgtgaatca atctcactgc aaaaccaagt ctccatgaat ctcgcaaaac acgtaatcac 
     1021 caccgtctct caaaactcca acgtcatctt ctcaccggct tcaatcaacg tcgtactcag 
     1081 tataatcgcc gctggatccg ccggcgctac caaagatcag atcctctcgt ttctcaaatt 
     1141 ctcttccact gatcaactta attcattctc ttccgaaatc gtctccgctg ttctcgctga 
     1201 cggtagtgct aacggtggtc ctaagctctc ggtggctaat ggcgcctgga tcgataagtc 
     1261 tctctccttt aaaccttcct ttaaacagct cttggaagat tcgtataaag ctgcttcgaa 
     1321 tcaagctgat tttcaatcga aggctgtgga ggtgattgct gaagtgaatt catgggctga 
     1381 aaaggagaca aatggtctca tcactgaggt tcttccagaa ggatcagctg atagtatgac 
     1441 caaactgata tttgcaaatg cattgtactt caagggaaca tggaacgaga aattcgatga 
     1501 gtcgttaaca caagaaggcg agtttcacct tcttgacggt aacaaagtga ctgcaccatt 
     1561 catgaccagc aagaagaaac aatacgtaag tgcttacgat ggtttcaaag ttttgggact 
     1621 tccttactta caaggacagg ataagcgaca attctccatg tacttttatc ttcccgatgc 
     1681 aaacaacgga ctgtctgatc ttctggacaa aatagtttcc actcctgggt tcttagacaa 
     1741 ccacatccca cgcagacaag ttaaagtccg cgaattcaag attccaaagt ttaaattctc 
     1801 tttcgggttc gatgcttcaa atgttttaaa aggattggga ctgacttcgc ctttcagcgg 
     1861 tgaagaaggt ttaactgaga tggttgaatc tcctgagatg gggaagaatc tatgcgtatc 
     1921 gaacattttc cataaagcgt gtatcgaagt gaatgaagaa ggaacagaag ctgcagctgc 
     1981 atcagctgga gttataaagc tagcaggatt gcttatggag gaagatgaaa tagattttgt 
     2041 tgcagaccat ccgtttctat tggtggtcac agagaacata acaggagtgg ttctgttcat 
     2101 tggccaagtt gttgatccgt tgcattaagg gcccgtttaa acccgctgat cagcctcgac 
     2161 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 
     2221 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 
     2281 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 
     2341 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag 
     2401 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc 
     2461 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 
     2521 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 
     2581 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 
     2641 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 
     2701 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 
     2761 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt 
     2821 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag 
     2881 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct 
     2941 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 
     3001 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 
     3061 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 
     3121 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 
     3181 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 
     3241 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 
     3301 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 
     3361 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 
     3421 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 
     3481 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 
     3541 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 
     3601 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 
     3661 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 
     3721 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 
     3781 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 
     3841 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 
     3901 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 
     3961 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 
     4021 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 
     4081 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 
     4141 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 
     4201 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 
     4261 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 
     4321 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 
     4381 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 
     4441 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 
     4501 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 
     4561 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     4621 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     4681 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
     4741 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     4801 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     4861 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     4921 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     4981 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 
     5041 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     5101 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     5161 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     5221 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     5281 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     5341 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     5401 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     5461 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     5521 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     5581 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     5641 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     5701 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     5761 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     5821 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     5881 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     5941 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     6001 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     6061 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     6121 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     6181 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     6241 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     6301 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     6361 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     6421 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     6481 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     6541 ccccgaaaag tgccacctga cgtc 
//