LOCUS       pCD-F-(vaccinia)SPI-3        6498 bp    DNA     circular     20-MAY-2008
DEFINITION  LMBP 6029.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|482867558|
COMMENT     VNTDBDATE|482867558|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCD-F-(vaccinia)SPI-3|
COMMENT     VNTAUTHORNAME|Jens Staal|
COMMENT     VNTAUTHOREML|jens.staal@dmbr.ugent.be|
COMMENT     VNTAUTHORWWW|www.dmbr.ugent.be|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     polyA_signal    2087..2301
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      2364..2777
                     /vntifkey="33"
                     /label=f1
     promoter        2842..3167
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     3203..3997
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    4013..4252
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      4684..5357
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(5502..6363)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     primer          complement(2085..2101)
                     /vntifkey="27"
                     /label=1503
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     CDS             953..2062
                     /vntifkey="4"
                     /label=SPI-3
BASE COUNT     1623 a      1570 c      1578 g      1727 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctatgattgc 
      961 gttattgata ctatcgttaa cgtgttcagt gtctacctat cgtctgcaag gatttaccaa 
     1021 tgccggtata gtagcgtata aaaatattca agatgataat attgtcttct caccgtttgg 
     1081 ttattcgttt tctatgttta tgtcgctatt gcctgcatca ggtaatacta gaatagaatt 
     1141 attgaagact atggatttga gaaaaagaga tctgggtcca gcatttacag aattaatatc 
     1201 aggattagct aagctgaaaa catctaaata tacgtacact gatctaactt atcaaagttt 
     1261 cgtagataat actgtgtgca ttaaaccgtc gtattatcaa caatatcata gattcggcct 
     1321 atatagatta aactttagac gagatgcggt taataaaatt aattctatag tagaacgtag 
     1381 atccggtatg tctaatgtag tagattctaa tatgctcgac aataatactc tatgggcaat 
     1441 cattaatact atatatttta aaggtatatg gcaatatccg tttgatatca ctaaaacacg 
     1501 caatgctagt tttactaata agtacggtac gaaaacggtt cccatgatga acgtagttac 
     1561 taaattgcaa ggaaatacaa tcacaatcga tgacgaagaa tatgatatgg tacgccttcc 
     1621 gtataaggat gctaatatta gtatgtacct ggcaataggt gataatatga cccatttcac 
     1681 agattctatt acggctgcaa aattagacta ttggtcgttt caattaggga ataaagtgta 
     1741 caatcttaaa ctccctaaat tttctatcga aaataagagg gatattaagt cgatagccga 
     1801 aatgatggct cctagtatgt ttaatccaga taatgcgtcg tttaaacata tgactaggga 
     1861 cccattatat atttataaaa tgtttcagaa tgcaaagata gatgtcgacg aacaaggaac 
     1921 tgtagcagag gcatctacta ttatggtagc tacggcgaga tcatctcctg aaaaactgga 
     1981 atttaataca ccatttgtgt tcatcatcag acatgatatt actggattta tattgtttat 
     2041 gggtaaggtg gaatctcctt aagggcccgt ttaaacccgc tgatcagcct cgactgtgcc 
     2101 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 
     2161 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 
     2221 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 
     2281 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 
     2341 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     2401 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     2461 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     2521 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     2581 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     2641 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     2701 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa 
     2761 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 
     2821 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta 
     2881 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     2941 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     3001 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     3061 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     3121 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 
     3181 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     3241 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     3301 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     3361 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 
     3421 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     3481 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     3541 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     3601 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     3661 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     3721 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     3781 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     3841 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     3901 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     3961 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 
     4021 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 
     4081 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 
     4141 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 
     4201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     4261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 
     4321 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 
     4381 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 
     4441 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     4501 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     4561 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     4621 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 
     4681 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     4741 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     4801 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     4861 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     4921 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     4981 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     5041 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     5101 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     5161 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 
     5221 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     5281 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     5341 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     5401 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     5461 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     5521 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     5581 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     5641 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     5701 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     5761 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     5821 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     5881 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     5941 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     6001 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     6061 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     6121 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     6181 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     6241 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     6301 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     6361 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     6421 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     6481 aaagtgccac ctgacgtc 
//