LOCUS       pCD-LCK-HLH-F-mMALT1-K10R        8475 bp    DNA     circular     22-FEB-2019
DEFINITION  LMBP 6042.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12697367|
COMMENT     VNTDBDATE|-12697367|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCD-LCK-HLH-F-mMALT1-K10R|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Jens Staal|
COMMENT     VNTOAUTHOREML|jens.staal@dmbr.ugent.be|
COMMENT     VNTOAUTHORWWW|www.dmbr.ugent.be|
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FEATURES             Location/Qualifiers
     misc_feature    7962..8444
                     /vntifkey="21"
                     /label=HLH
     promoter        7203..7857
                     /vntifkey="29"
                     /label=pCMV
     promoter        7857..7876
                     /vntifkey="30"
                     /label=T7
     polyA_signal    2583..2797
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      2860..3273
                     /vntifkey="33"
                     /label=f1
     promoter        3338..3663
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     3699..4493
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    4509..4748
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      5180..5853
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(5998..6859)
                     /vntifkey="22"
                     /label=AmpR
     CDS             63..2558
                     /vntifkey="4"
                     /label=mMALT1\mutant
BASE COUNT     2060 a      2147 c      2152 g      2116 t 
ORIGIN
        1 caggcctgaa cgacatcttc gaggcccaga agatcgagtg gcacgaggag gatcctgcca 
       61 ccatgtcgct gtgggggcag ccgctgcagg cctcacctcc cctggccgta cggcagcccc 
      121 ctaccgcgtc ctcgggacct tcgacctccc ctccagccgg cgcaaccctc aaccgcctgc 
      181 ccgagccgct gctgcggagg ctcagcgaga gcttggaccg cgctccggag ggccggggct 
      241 ggcggcagct ggccgagctg gcgggcagtc ggggacgcct ccggctcagt ggcctggacc 
      301 tggaacagtg ttctcttaaa gtattggagc cagagggaag cccaagtttg tgtttattga 
      361 aactaatggg tgaaaaagga tgtacagtca cagaactgag cgacttccta caggctctcg 
      421 agcacactga ggttcttccg cttctcaacc ccccaggatt aaagatcact gtaaatccgg 
      481 aatccaaggc agtgttggct ggacagtttg taaaactgtg ctgccgggca actggacatc 
      541 cttttgtaca atatcaatgg ttcaaaatga ataaagagat cccatatgga aattcatcag 
      601 aacttgtttt taacacagtg catgtaaaag atgcaggctt ttatgtctgc cgggttaaca 
      661 acagttccac ttttgaattt agccagtggt cacagctgga tgtgtgtgat gtggcagaag 
      721 taacagacag cttccaggga agtatggatg gcatctcaga atccaggttg caaatctgtg 
      781 ttgaaccaag gtcccaaagg ctggtgccag gcagcatgtt gttgttacag tgtgttgcta 
      841 ttggaagtcc tatgcctcac taccagtggt ttaaagatga atcaccatta acacatgaga 
      901 caaaaaagca ctacacggtt ccatatgtgg atatagaaca tgaaggaacc tactggtgtc 
      961 atgtatataa tgaccgagac agtcaagata gcaagaaggc agaagtcacc ataggaagaa 
     1021 cagatgaggc agtggagtgc actgaagatg aattaaacaa tttggggcat cctgataata 
     1081 aagaacaaac tggccagcct ttggcaaagg acaaagtcgc ccttttgata gggaatatga 
     1141 gttactggga gcatcccaag cttaaggcgc ctttggtgga tgtgtatgaa ttgaccaact 
     1201 tactaagaca actagatttc aaagttgtct cactgttgga ccttactgaa tatgagatgt 
     1261 gtaacgctgt ggatgaattt ttactacttt tagacaaagg agtatatgga ttgttatatt 
     1321 atgcagggca cggttatgaa aactttggaa acagtttcat ggtccctgtt gatgctccaa 
     1381 atccatatag gtctgaaaat tgcctatgcg tacaaaacat actgaaatta atgcaagaaa 
     1441 aggagactgg cctgaatgtg ttcctgttgg atatgtgtcg gaaaagaaat gactacgatg 
     1501 ataccattcc aatcttggac gcactgaaag tcactgccaa cattgtgttt ggatatgcca 
     1561 cgtgccaagg agcagaagcc tttgaaatcc agcattctgg gttggcaaat gggattttta 
     1621 tgaaattttt aaaagataga ttattagaag acaagaaaat tactgtgtta ctggatgaag 
     1681 ttgcagaaga tatgggtaaa tgtcacctta ccaaaggcag acaagctcta gagattcgca 
     1741 gcagcttgtc tgaaaagaga gcacttaccg atccagtcca gggagcacct tgctctgcag 
     1801 aggctctggt gcgcaatcta cagtgggcca aggctcatga acttccagaa agtatgtgcc 
     1861 ttaaatttca gtgtggcgtt cacattcaat taggatttgc cgcagagttc tccaatgtca 
     1921 tgataatcta cacaagtata gtccacaaac cacctgagat aataatgtgt gacgcctatg 
     1981 ttactgattt ccctcttgac ctagatattg atccgcggca tgcaaacagg gggactcctg 
     2041 aagaaactgg cagctactta gtatcgcgag accttcctag gcactgcctc tacacgcgtc 
     2101 tcagctcact acagcgctta agggaacatc tgatcttcac agtctgtctg tcctatcagt 
     2161 attcaggact agaagacact gtggaggaga ggcaggaggt gaacgtgggg aggcctctta 
     2221 ttgcgcgcct agacatgcac cgaggactgg gcaggaggac ttgcttccaa gcttgtcgta 
     2281 tgcctgacga gccgtaccac agctccacat ctacctcagc gggagcgggg catttccatt 
     2341 cctctcagga ctcattccat gatgtgtacc attcacatct tggcaatgca gacagtggta 
     2401 tgccaccaga caggtgtcat tgcagccgga ctccacacac attcatttca aattatcccc 
     2461 cccaccacta ctgccagttt ggtagaagta atgtgcccgt agagacaact gatgaaatgc 
     2521 cattcagttt ttctgacagg cttatgattt ctgaaaacgg gcccgtttaa acccgctgat 
     2581 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 
     2641 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 
     2701 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 
     2761 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 
     2821 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat 
     2881 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 
     2941 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 
     3001 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc 
     3061 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 
     3121 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 
     3181 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg 
     3241 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa 
     3301 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa 
     3361 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
     3421 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     3481 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     3541 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 
     3601 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 
     3661 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 
     3721 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 
     3781 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 
     3841 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 
     3901 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 
     3961 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 
     4021 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 
     4081 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 
     4141 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 
     4201 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 
     4261 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 
     4321 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 
     4381 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 
     4441 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 
     4501 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 
     4561 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 
     4621 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 
     4681 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     4741 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 
     4801 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 
     4861 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 
     4921 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 
     4981 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 
     5041 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     5101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     5161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     5221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     5281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     5341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     5401 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 
     5461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     5521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     5581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     5641 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 
     5701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     5761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     5821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     5881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
     5941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
     6001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
     6061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
     6121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
     6181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
     6241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
     6301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
     6361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
     6421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
     6481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
     6541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
     6601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
     6661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
     6721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
     6781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
     6841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 
     6901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 
     6961 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc 
     7021 ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc 
     7081 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg 
     7141 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt 
     7201 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa 
     7261 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 
     7321 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 
     7381 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggactattta 
     7441 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 
     7501 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 
     7561 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 
     7621 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 
     7681 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 
     7741 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 
     7801 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat 
     7861 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt atgggctgtg 
     7921 tctgcagctc aaaccctgaa gatgactggg atcagcttac catggcgctg ttgggggacc 
     7981 cgctacaggc cctgccgccc tcggccgccc ccacggggcc gctgctcgcc cctccggccg 
     8041 gcgcgaccct caaccgcctg cgggagccgc tgctgcggag gctcagctac gcttcctcga 
     8101 cgccacttca tgttccagtg cctcgagcgc tcaggatgga ggaagactcg atccgcctgc 
     8161 ctgcgcacct gcgcttgcag ccaatttact ggagcaggga tgacgtagcc cagtggctca 
     8221 agtgggctga aaatgagttt tctttaaggc caattgacag caacacgttt gaaatgaatg 
     8281 gcaaagctct cctgctgctg accaaagagg actttcgcta tcgatctcct cattcaggtg 
     8341 atgtgctcta tgaactcctt cagcatattc tgaagcagag gaaacctcgg attctttttt 
     8401 caccattctt ccaccctgga aactctatac acacacagcc ggttatggat tacaaggacg 
     8461 atgacgataa ggtac 
//
