LOCUS       pCD-LCK-HLH-F-MALT1        8451 bp    DNA     circular      6-JUN-2008
DEFINITION  LMBP 6053.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|484420535|
COMMENT     VNTDBDATE|484420535|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCD-LCK-HLH-F-MALT1|
COMMENT     VNTAUTHORNAME|Jens Staal|
COMMENT     VNTAUTHOREML|jens.staal@dmbr.ugent.be|
COMMENT     VNTAUTHORWWW|www.dmbr.ugent.be|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT             (CTerm 1 1 0 "ApaI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
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COMMENT             "pCD-LCK-HLH-F-SEQ" "" 0 0 1 5912 2540 8451 1 5912 0 "" "" "" "" "" 
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COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
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COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 51 0 0 "") 
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COMMENT        #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 658 0 0 "") 
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COMMENT        #20=(CFSignal (CObList) "HLH" 21 0 0 7938 8420 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "pCMV" 29 0 0 7179 7833 1 (CStringList) 
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COMMENT        #22=(CFSignal (CObList) "T7" 30 0 0 7833 7852 1 (CStringList) 
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FEATURES             Location/Qualifiers
     CDS             63..2534
                     /vntifkey="4"
                     /label=MK
     protein_bind    2472..2489
                     /vntifkey="31"
                     /label=TRAF6
     mutation        2012..2012
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation, G replaces A"
     modified_base   1550..1550
                     /vntifkey="23"
                     /label=stability\creator
                     /note="T replaces A as GCAGAAG recombines with the same heptanucleotide present in API2"
     protein_bind    2022..2153
                     /vntifkey="31"
                     /label=VDJ4
     protein_bind    204..428
                     /vntifkey="31"
                     /label=DD
                     /note="Death Domain"
     protein_bind    738..944
                     /vntifkey="31"
                     /label=IgL2
     protein_bind    453..686
                     /vntifkey="31"
                     /label=IgL1
     misc_feature    7938..8420
                     /vntifkey="21"
                     /label=HLH
     promoter        7179..7833
                     /vntifkey="29"
                     /label=pCMV
     promoter        7833..7852
                     /vntifkey="30"
                     /label=T7
     polyA_signal    2559..2773
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      2836..3249
                     /vntifkey="33"
                     /label=f1
     promoter        3314..3639
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     3675..4469
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    4485..4724
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      5156..5829
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(5974..6835)
                     /vntifkey="22"
                     /label=AmpR
BASE COUNT     2089 a      2081 c      2120 g      2161 t 
ORIGIN
        1 caggcctgaa cgacatcttc gaggcccaga agatcgagtg gcacgaggag gatcctgcca 
       61 ccatggcgct gttgggggac ccgctacagg ccctgccgcc ctcggccgcc cccacggggc 
      121 cgctgctcgc ccctccggcc ggcgcgaccc tcaaccgcct gcgggagccg ctgctgcgga 
      181 ggctcagcga gctcctggat caggcgcccg agggccgggg ctggaggaga ctggcggagc 
      241 tggcggggag tcgcgggcgc ctccgcctca gttgcctaga cctggagcag tgttctctta 
      301 aggtactgga gcctgaagga agccccagcc tgtgtctgct gaagttaatg ggtgaaaaag 
      361 gttgcacagt cacagaattg agtgatttcc tgcaggctat ggaacacact gaagttcttc 
      421 agcttctcag ccccccagga ataaagatta ctgtaaaccc agagtcaaag gcagtcttgg 
      481 ctggacagtt tgtgaaactg tgttgccggg caactggaca tccttttgtt caatatcagt 
      541 ggttcaaaat gaataaagag attccaaatg gaaatacatc agagcttatt tttaatgcag 
      601 tgcatgtaaa agatgcaggc ttttatgtct gtcgagttaa taacaatttc acctttgaat 
      661 tcagccagtg gtcacagctg gatgtttgcg acatcccaga gagcttccag agaagtgttg 
      721 atggcgtctc tgaatccaag ttgcaaatct gtgttgaacc aacttcccaa aagctgatgc 
      781 caggcagcac attggtttta cagtgtgttg ctgttggaag ccctattcct cactaccagt 
      841 ggttcaaaaa tgaattacca ttaacacatg agaccaaaaa gctatacatg gtgccttatg 
      901 tggatttgga acaccaagga acctactggt gtcatgtata taatgatcga gacagtcaag 
      961 atagcaagaa ggtagaaatc atcataggaa gaacagatga ggcagtggag tgcactgaag 
     1021 atgaattaaa taatcttggt catcctgata ataaagagca aacaactgac cagcctttgg 
     1081 cgaaggacaa ggttgccctt ttgataggaa atatgaatta ccgggagcac cccaagctca 
     1141 aagctccttt ggtggatgtg tacgaattga ctaacttact gagacagctg gacttcaaag 
     1201 tggtttcact gttggatctt actgaatatg agatgcgtaa tgctgtggat gagtttttac 
     1261 tccttttaga caagggagta tatgggttat tatattatgc aggacatggt tatgaaaatt 
     1321 ttgggaacag cttcatggtc cccgttgatg ctccaaatcc atataggtct gaaaattgtc 
     1381 tgtgtgtaca aaatatactg aaattgatgc aagaaaaaga aactggactt aatgtgttct 
     1441 tattggatat gtgtaggaaa agaaatgact acgatgatac cattccaatc ttggatgcac 
     1501 taaaagtcac cgccaatatt gtgtttggat atgccacgtg tcaaggagct gaagcttttg 
     1561 aaatccagca ttctggattg gcaaatggaa tctttatgaa atttttaaaa gacagattat 
     1621 tagaagataa gaaaatcact gtgttactgg atgaagttgc agaagatatg ggtaagtgtc 
     1681 accttaccaa aggcaaacag gctctagaga ttcgaagtag tttatctgag aagagagcac 
     1741 ttactgatcc aatacaggga acagaatatt ctgctgaatc tcttgtgcgg aatctacagt 
     1801 gggccaaggc tcatgaactt ccagaaagta tgtgtcttaa gtttgactgt ggtgttcaga 
     1861 ttcaattagg atttgcagct gagttttcca atgtcatgat catctataca agtatagttt 
     1921 acaaaccacc ggagataata atgtgtgatg cctacgttac tgattttcca cttgatctag 
     1981 atattgatcc aaaagatgca aataaaggca cgcctgaaga aactggcagc tacttggtat 
     2041 caaaggatct tcccaagcat tgcctctata ccagactcag ttcactgcaa aaattaaagg 
     2101 aacatctagt cttcacagta tgtttatcat atcagtactc aggattggaa gatactgtag 
     2161 aggacaagca ggaagtgaat gttgggaaac ctctcattgc taaattagac atgcatcgag 
     2221 gtttgggaag gaagacttgc tttcaaactt gtcttatgtc taatggtcct taccagagtt 
     2281 ctgcagccac ctcaggagga gcagggcatt atcactcatt gcaagaccca ttccatggtg 
     2341 tttaccattc acatcctggt aatccaagta atgttacacc agcagatagc tgtcattgca 
     2401 gccggactcc agatgcattt atttcaagtt tcgctcacca tgcttcatgt cattttagta 
     2461 gaagtaatgt gccagtagag acaactgatg aaataccatt tagtttctct gacaggctca 
     2521 gaatttctga aaaagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt 
     2581 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 
     2641 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 
     2701 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 
     2761 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 
     2821 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
     2881 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
     2941 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt 
     3001 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 
     3061 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
     3121 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
     3181 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga 
     3241 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 
     3301 gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 
     3361 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 
     3421 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 
     3481 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 
     3541 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 
     3601 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 
     3661 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     3721 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     3781 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     3841 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     3901 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     3961 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     4021 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     4081 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     4141 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     4201 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     4261 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     4321 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     4381 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     4441 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 
     4501 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 
     4561 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 
     4621 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 
     4681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     4741 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 
     4801 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     4861 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 
     4921 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 
     4981 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
     5041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     5101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     5161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     5221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     5281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     5341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     5401 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     5461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     5521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     5581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     5641 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     5701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     5761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     5821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     5881 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     5941 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     6001 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     6061 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     6121 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     6181 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     6241 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     6301 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     6361 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     6421 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     6481 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     6541 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     6601 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     6661 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     6721 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     6781 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     6841 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     6901 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     6961 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc 
     7021 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct 
     7081 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg 
     7141 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg 
     7201 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat 
     7261 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     7321 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 
     7381 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta 
     7441 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 
     7501 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 
     7561 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 
     7621 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 
     7681 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 
     7741 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact 
     7801 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa 
     7861 gctggctagc gtttaaactt aagcttatgg gctgtgtctg cagctcaaac cctgaagatg 
     7921 actgggatca gcttaccatg gcgctgttgg gggacccgct acaggccctg ccgccctcgg 
     7981 ccgcccccac ggggccgctg ctcgcccctc cggccggcgc gaccctcaac cgcctgcggg 
     8041 agccgctgct gcggaggctc agctacgctt cctcgacgcc acttcatgtt ccagtgcctc 
     8101 gagcgctcag gatggaggaa gactcgatcc gcctgcctgc gcacctgcgc ttgcagccaa 
     8161 tttactggag cagggatgac gtagcccagt ggctcaagtg ggctgaaaat gagttttctt 
     8221 taaggccaat tgacagcaac acgtttgaaa tgaatggcaa agctctcctg ctgctgacca 
     8281 aagaggactt tcgctatcga tctcctcatt caggtgatgt gctctatgaa ctccttcagc 
     8341 atattctgaa gcagaggaaa cctcggattc ttttttcacc attcttccac cctggaaact 
     8401 ctatacacac acagccggtt atggattaca aggacgatga cgataaggta c 
//
