LOCUS       pCAGGS-E-mXedar-E232K-E256K        5761 bp    DNA     circular     29-MAR-2007
DEFINITION  LMBP 6060.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|442142843|
COMMENT     VNTNAME|pCAGGS-E-mXedar-E232K-E256K|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         "KpnI" "MfeI" "NcoI" "NotI" "PstI" "PvuI" "SmaI" "SphI" "StuI" "TaqI" 
COMMENT         "XbaI" "XhoI" "XmaI") (CStringList "atg" "gtg") 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 1 3097 0 0 "") 
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COMMENT        #2=(CRSite (CStringList) "DpnI" "gatc" 3 0 19 542 0 680 0 1038 0 1483 0 
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COMMENT            0 3420 0 3466 0 3724 0 3741 0 3777 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 956 0 5750 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3319 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1502 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 801 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 753 0 1779 0 4395 0 5758 
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COMMENT        #11=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 348 0 716 0 1497 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3467 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "StuI" "aggcct" 4 0 1 1871 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "TaqI" "tcga" 2 0 9 131 0 945 0 951 0 1488 0 
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COMMENT        #17=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 5649 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 944 0 0 "") 
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COMMENT        #22=(CFSignal (CObList) "hCMV" 29 0 0 4019 4396 0 (CStringList) 
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COMMENT        #23=(CFSignal (CObList) "AMP" 4 0 1 3023 3883 0 (CStringList) 
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COMMENT        #24=(CFSignal (CObList) "ORI" 33 0 1 2081 2482 0 (CStringList) 
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COMMENT        #26=(CFSignal (CObList) "polyA" 43 0 0 1892 1966 0 (CStringList) 
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COMMENT                          "Created: 03/29/07 09:27" 1) 
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COMMENT                          "Last Modified: 03/29/07 09:27" 1) 
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COMMENT                          "length: 5761 bp" 1) 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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FEATURES             Location/Qualifiers
     intron          4771..5695
                     /vntifkey="15"
                     /label=INT
                     /note="chicken ç-actin/ rabbit ç-globin hybrid intron"
     promoter        4402..5743
                     /vntifkey="30"
                     /label=AG
                     /note="chicken ç-actin/ rabbit ç-globin hybrid promoter"
     promoter        4019..4396
                     /vntifkey="29"
                     /label=hCMV
                     /note="human CMV-IE enhancer"
     CDS             complement(3023..3883)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(2081..2482)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     terminator      complement(1892..1966)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     terminator      1892..1966
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     rep_origin      1786..1861
                     /vntifkey="33"
                     /label=SVORI
                     /note="bidirectional origin of SV40"
     promoter        complement(1562..1644)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     misc_feature    1128..1484
                     /vntifkey="21"
                     /label=3FR
                     /note="3' flanking region of the rabbit ç-globin gene"
     terminator      1036..1127
                     /vntifkey="43"
                     /label=polyA
                     /note="3' UTR of the rabbit ç-globin gene, incl. polyA"
     CDS             1..39
                     /vntifkey="4"
                     /label=E-tag
                     /note="E-tag"
     CDS             49..942
                     /vntifkey="4"
                     /label=mouse\XEDAR
BASE COUNT     1240 a      1529 c      1576 g      1416 t 
ORIGIN
        1 ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgcaat ggattgtcaa 
       61 gagaatgagt accgggacca atgggggcgc tgtgtcacct gccaacaatg tggccctgga 
      121 caggagctct cgaaggattg tggttatgga gaaggtggag atgcacactg catagtctgc 
      181 cctccccgaa agtataaaag cacttgggga catcacagat gtcagacatg catcacttgt 
      241 gctgtcatca atcgggtcca gaaggccaac tgcacaaata cctctaatgc tatctgtgga 
      301 gactgtctgc ccaggttcta ccgaaagaca cgcattgggg gcctgcagga ccaagaatgc 
      361 atcccatgta caaagcagac tccttcttcc gaggttcagt gtaccttcca gttgagctta 
      421 gtgaaggtag atgcacacac tgttccccct agggaagcca cacttgttgc actggtggga 
      481 agtctgctag tggtatttgc tctggccttc cttggactct tcttccttta ttgcaagcag 
      541 atcttcaaca gacattgtca atgcagagat tcgttgcaat atgaggctga aaagacagtg 
      601 gaggaggatt ctcttttccc cgtaccacct ggccaggaga ccagtcctga atttccagct 
      661 aatgagggca tcttggagat caaaccactt aactccatcc tggatgatga ctgcagctcc 
      721 actcgcggct tccccaccca gaagtccttt accatggcct cctgtgcctc agagagccat 
      781 tctcaatggg tccatacccc aattgaatgt acaaagctgg acctgcaaaa gttttccagc 
      841 tctatcccct ctactggacc tgaaaccttg agagagaaca cagctgaaca ctcaggagac 
      901 aggctggagc tctatgtacc ttttgaagtt cccagccttt aactcgaggt cgaggaattc 
      961 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct 
     1021 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc 
     1081 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg 
     1141 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag 
     1201 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa 
     1261 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc 
     1321 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt 
     1381 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc 
     1441 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc 
     1501 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     1561 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc 
     1621 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     1681 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 
     1741 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     1801 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 
     1861 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta 
     1921 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     1981 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga 
     2041 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     2101 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     2161 gtaatacggt tatccacaga atcagggata acgcaggaaa gaacatgtga gcaaaaggcc 
     2221 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     2281 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     2341 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     2401 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     2461 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     2521 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     2581 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     2641 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     2701 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     2761 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     2821 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     2881 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     2941 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     3001 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     3061 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     3121 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     3181 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     3241 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     3301 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     3361 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     3421 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     3481 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     3541 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     3601 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     3661 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     3721 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     3781 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     3841 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     3901 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     3961 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga 
     4021 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 
     4081 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 
     4141 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 
     4201 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 
     4261 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 
     4321 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 
     4381 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc 
     4441 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg 
     4501 ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga ggggcggggc 
     4561 ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct 
     4621 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga 
     4681 gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc 
     4741 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg 
     4801 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc 
     4861 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg 
     4921 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc 
     4981 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc 
     5041 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc 
     5101 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc 
     5161 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc 
     5221 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 
     5281 ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg 
     5341 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca 
     5401 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc 
     5461 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg 
     5521 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg 
     5581 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac 
     5641 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct 
     5701 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattga attccaccat 
     5761 g 
//
