LOCUS       pCAGGS-E-mCASP-1-4DE        5981 bp    DNA     circular     27-JAN-2014
DEFINITION  LMBP 6093.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6093
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 27-JAN-2014
COMMENT     VNTDATE|629479347|
COMMENT     VNTNAME|pCAGGS-E-mCASP-1-4DE|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-mCASP-1-4DE" 0 5981 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BglII" "HindIII" "NcoI" "NotI" "PvuI" "BalI" "KpnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 1769 1768 1 5981 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 1271 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 871 0 1737 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 12 0 2014 0 4630 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3702 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BalI" "tggcca" 4 0 1 381 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 432 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "3' UTR of \337-globin, incl. polyA" 43 0 0 1271 
COMMENT            1362 0 (CStringList) (CStringList) 1 1 1 1 "1271..1362") 
COMMENT        #8=(CFSignal (CObList) "SV40 ori" 33 0 0 2021 2096 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2021..2096") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2127 2201 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2127..2201") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2127 2201 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2127..2201)") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 2316 2717 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2316..2717)") 
COMMENT        #12=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4254 4631 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4254..4631") 
COMMENT        #13=(CFSignal (CObList) "AG promoter" 29 0 0 4637 5978 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4637..5978") 
COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3258 4118 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3258..4118)") 
COMMENT        #15=(CFSignal (CObList) "intron AG promoter" 15 0 0 5006 5930 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5006..5930") 
COMMENT        #16=(CFSignal (CObList) "3' flanking region rabbit \337-globin" 21 0 0 
COMMENT             1363 1719 0 (CStringList) (CStringList) 1 1 1 1 "1363..1719") 
COMMENT        #17=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #18=(CFSignal (CObList) "lac promoter" 30 0 1 1797 1879 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1797..1879)") 
COMMENT        #19=(CFSignal (CObList) "mCASP-1 mutant" 4 0 0 64 1269 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "61..1269") 
COMMENT        #20=(CFSignal (CObList) "D103E" 62 0 0 367 369 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "367..369") 
COMMENT        #21=(CFSignal (CObList) "D122E" 62 0 0 424 426 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "424..426") 
COMMENT        #22=(CFSignal (CObList) "D296E" 62 0 0 946 948 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "946..948") 
COMMENT        #23=(CFSignal (CObList) "D314E" 62 0 0 1000 1002 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1000..1002")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #24=(CFSignal (CObList) "LMBP 6093" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #25=(CFSignal (CObList) "Last modified: 27-JAN-2014" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #26=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #27=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "pCAGGS-E-mCASP-1-4DE" 2) 5 "" 0 4) 
COMMENT              #28=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGS-E-mCASP-1-4DE'" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 6093." 
COMMENT                          1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: Kristof Kersse" 
COMMENT                          1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/21/12 03:22" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/27/14 09:23" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5981 bp" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 20-JAN-2014" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #40=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Kristof Kersse" 1))) "Original Author") 
COMMENT              #42=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #43=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CLinePar (CParagraph 0 (15 #24# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 6093") 
COMMENT                     #46=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #47=(CLinePar (CParagraph 0 (15 #25# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 27-JAN-2014"))) 
COMMENT                   "Annotations") 
COMMENT              #48=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #49=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #50=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 64 (3 #19# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 64    End: 1269" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   61..1269" 1))) "mCASP-1 mutant") 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3258 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3258  End: 4118 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3258..4118)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
COMMENT                     #58=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #59=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5006 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5006  End: 5930" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid intron" 
COMMENT                                        1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5006..5930" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
COMMENT                     #64=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #65=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1363 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1363  End: 1719" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1363..1719" 1))) 
COMMENT                                 "3' flanking region rabbit \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #69=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 16 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 16    End: 54  " 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   16..54" 1))) "E-tag"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #74=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4254 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4254  End: 4631" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4254..4631" 1))) "hCMV-IE enhancer") 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4637 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4637  End: 5978" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4637..5978" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #84=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #85=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1797 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1797  End: 1879 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1797..1879)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #89=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #90=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2021 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2021  End: 2096" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2021..2096" 1))) "SV40 ori") 
COMMENT                            #94=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2316 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2316  End: 2717 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2316..2717)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
COMMENT                     #98=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #99=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1271 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Start: 1271  End: 1362" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   1271..1362" 1))) 
COMMENT                                 "3' UTR of \337-globin, incl. polyA") 
COMMENT                            #103=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 2127 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 2127  End: 2201" 1) 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   2127..2201" 1))) 
COMMENT                                  "SV40 polyA early") 
COMMENT                            #107=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 2127 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #108=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 2127  End: 2201 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #110=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(2127..2201)" 1))) 
COMMENT                                  "SV40 polyA late"))) "Terminator (3 total)") 
COMMENT                     #111=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 62 (7 62 0) 1 2 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #112=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 367 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 367   End: 369 " 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "WT: GAT" 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   367..369" 1))) "D103E") 
COMMENT                             #117=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 424 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 424   End: 426 " 1) 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "WT: GAT" 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   424..426" 1))) "D122E") 
COMMENT                             #122=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 946 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 946   End: 948 " 1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "WT: GAT" 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   946..948" 1))) "D296E") 
COMMENT                             #127=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1000 (3 #23# 0) 1 2 2 1 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1000  End: 1002" 1) 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "WT: GAT" 1) 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #131=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1000..1002" 1))) "D314E"))) 
COMMENT                           "Mutation (4 total)"))) "Feature Map") 
COMMENT              #132=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #133=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #134=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #135=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1271 " 1))))) "BglII: 1 site") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
COMMENT                      #136=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #137=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #138=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 57 " 1))))) "NotI: 1 site") 2 
COMMENT                            6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                      #139=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #140=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #141=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3702 " 1))))) "PvuI: 1 site") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
COMMENT                      #142=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #143=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #144=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 381 " 1))))) "BalI: 1 site") 3 
COMMENT                            3 "TGGCCA" "ACCGGT") 
COMMENT                      #145=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #146=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #147=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 432 " 1))))) "KpnI: 1 site") 5 
COMMENT                            1 "GGTACC" "CCATGG") 
COMMENT                      #148=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #149=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #150=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 871 " 1) 
COMMENT                                      #151=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1737 " 1))))) 
COMMENT                             "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #152=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #153=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #154=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 12 " 1) 
COMMENT                                      #155=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2014 " 1) 
COMMENT                                      #156=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4630 " 1))))) "NcoI: 3 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC"))) 
COMMENT                    "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #157=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #158=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #159=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #160=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #161=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #162=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #163=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) 
COMMENT          #164=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #165=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #166=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #167=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #168=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #169=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #170=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #171=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #172=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #173=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #174=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #175=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) #176=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #177=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #178=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #179=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #180=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #181=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #182=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #183=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #184=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #185=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #186=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #187=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #188=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #189=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #190=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #191=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #192=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #193=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #194=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #195=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #196=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #197=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #198=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #199=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #200=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #201=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #202=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #203=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #204=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #205=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #206=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #207=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #208=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #209=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #210=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #211=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #212=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #213=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #214=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #215=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #216=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 21.735 -20.5411 40.3368 40.3368 0.01 10 -1 5981 5981 1 0 0) 
COMMENT        #217=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 540689732 100) 
COMMENT              (CObjectList 
COMMENT               #218=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -793 100) 
COMMENT                     (CObjectList 
COMMENT                      #219=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #163# 27670700 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27523 
COMMENT                             6.27323 1 0.0214037) 0.104885) 
COMMENT                      #220=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #160# 0 100) 
COMMENT                            (LOGPEN 0 0 3381657) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pCAGGS-E-mCASP-1-4DE" "@N" 1 0 
COMMENT                            0.871165 0 -10 16.7589 1.56185 Nil) 
COMMENT                      #221=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #159# 760238913 100) 
COMMENT                            (LOGPEN 0 0 16724787) 1 
COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "5981 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 4.78471 1.80976 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #222=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -718 100) 
COMMENT                     (CObjectList 
COMMENT                      #223=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -532 100) 
COMMENT                            (CObjectList 
COMMENT                             #224=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #161# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    1.95401 2.85707 1 0.082322) 0.8 1.8 1) 
COMMENT                             #225=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ampicillin resistance" "@N" 1 -13.6456 
COMMENT                                   -12.4373 0 -10 8.03237 1.16519 #224#) 
COMMENT                             #226=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #19# 0) 1 2 0 0 #161# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.94219 6.20711 1 0.082322) 0.8 1.8 0) 
COMMENT                             #227=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "mCASP-1 mutant" 
COMMENT                                   "@N" 1 12.9357 11.7401 0 -10 6.7928 1.16519 
COMMENT                                   #226#)) (CObjectList)) 
COMMENT                      #228=(CGroupWidget 
COMMENT                            (CWidget 15 (7 15 0) 1 2 0 0 Nil -392 100) 
COMMENT                            (CObjectList 
COMMENT                             #229=(CLine 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #192# 701 100) 
COMMENT                                   (LOGPEN 0 0 3342336) 1 0.835356 0.0534915 
COMMENT                                   1.02368) 
COMMENT                             #230=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "intron AG promoter" "@N" 1 -11.612 13.0075 0 
COMMENT                                   -10 7.71008 1.16519 #229#)) (CObjectList)) 
COMMENT                      #231=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -742 100) 
COMMENT                            (CObjectList 
COMMENT                             #232=(CLine 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #164# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.934636 4.47021 
COMMENT                                   4.84465) 
COMMENT                             #233=(CLabel (CWidget 0 (0 0) 1 2 0 0 #202# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' flanking region rabbit \337-globin" "@N" 
COMMENT                                   1 21.5545 -2.08544 0 -10 12.7427 1.16519 
COMMENT                                   #232#)) (CObjectList)) 
COMMENT                      #234=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -683 100) 
COMMENT                            (CObjectList 
COMMENT                             #235=(CScratch 
COMMENT                                   (CWidget 0 (3 #17# 0) 1 2 0 0 #200# 1130 100) 
COMMENT                                   (LOGPEN 0 4 10040064) 6 1 6.23752 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #236=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "E-tag" "@N" 1 
COMMENT                                   1.90284 18.2275 0 -10 2.15684 1.16519 #235#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #237=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -718 100) 
COMMENT                            (CObjectList 
COMMENT                             #238=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #198# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    1.41595 1.81242 1 0.082322) 0.8 1.8 0) 
COMMENT                             #239=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hCMV-IE enhancer" "@N" 1 -16.2216 -1.48748 0 
COMMENT                                   -10 7.53654 1.16519 #238#) 
COMMENT                             #240=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #198# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    1.00728 0.00314656 1.41071 1 0.082322) 0.8 
COMMENT                                   1.8 0) 
COMMENT                             #241=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AG promoter" 
COMMENT                                   "@N" 1 -11.6121 11.0916 0 -10 5.23096 1.16519 
COMMENT                                   #240#)) (CObjectList)) 
COMMENT                      #242=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -953 100) 
COMMENT                            (CObjectList 
COMMENT                             #243=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #203# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    4.30239 4.38945 1 0.082322) 0.8 1.8 1) 
COMMENT                             #244=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 17.636 -6.48738 0 -10 5.05742 1.16519 
COMMENT                                   #243#)) (CObjectList)) 
COMMENT                      #245=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -250 100) 
COMMENT                            (CObjectList 
COMMENT                             #246=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #173# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    4.07479 4.1545 1 0.082322) 0.8 1.8 0) 
COMMENT                             #247=(CLabel (CWidget 0 (0 0) 1 2 0 0 #201# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 15.598 -10.8645 0 -10 3.56994 1.16519 #246#) 
COMMENT                             #248=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #173# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    3.42345 3.84509 1 0.082322) 0.8 1.8 1) 
COMMENT                             #249=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #201# 1195852611 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 8.74057 -19.1656 0 -10 3.56994 1.16519 
COMMENT                                   #248#)) (CObjectList)) 
COMMENT                      #250=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -1094 100) 
COMMENT                            (CObjectList 
COMMENT                             #251=(CLine 
COMMENT                                   (CWidget 0 (3 #7# 0) 1 2 0 0 #164# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.896371 4.84465 
COMMENT                                   4.94114) 
COMMENT                             #252=(CLabel (CWidget 0 (0 0) 1 2 0 0 #211# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' UTR of \337-globin, incl. polyA" "@N" 1 
COMMENT                                   20.4969 2.13601 0 -10 11.8254 1.16519 #251#) 
COMMENT                             #253=(CLine 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #164# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.902402 3.96466 
COMMENT                                   4.04333) 
COMMENT                             #254=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #211# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 16.2948 -12.6316 0 
COMMENT                                   -10 7.11509 1.16519 #253#) 
COMMENT                             #255=(CLine 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #164# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.799265 3.96466 
COMMENT                                   4.04333) 
COMMENT                             #256=(CLabel (CWidget 0 (0 0) 1 2 0 0 #211# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 15.2346 -14.6219 0 
COMMENT                                   -10 6.54489 1.16519 #255#)) (CObjectList)) 
COMMENT                      #257=(CGroupWidget 
COMMENT                            (CWidget 62 (7 62 0) 1 2 0 0 Nil -1023 100) 
COMMENT                            (CObjectList 
COMMENT                             #258=(CScratch 
COMMENT                                   (CWidget 0 (3 #20# 0) 1 2 0 0 #166# 454 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.88931 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #259=(CLabel (CWidget 0 (0 0) 1 2 0 0 #210# 0 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "D103E" "@N" 1 
COMMENT                                   7.24559 16.6518 0 -10 2.75183 1.06602 #258#) 
COMMENT                             #260=(CScratch 
COMMENT                                   (CWidget 0 (3 #21# 0) 1 2 0 0 #166# 1130 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.82952 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #261=(CLabel (CWidget 0 (0 0) 1 2 0 0 #210# 0 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "D122E" "@N" 1 
COMMENT                                   8.44229 14.4067 0 -10 2.75183 1.06602 #260#) 
COMMENT                             #262=(CScratch 
COMMENT                                   (CWidget 0 (3 #22# 0) 1 2 0 0 #166# 454 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.28202 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #263=(CLabel (CWidget 0 (0 0) 1 2 0 0 #210# 0 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "D296E" "@N" 1 
COMMENT                                   14.7224 8.52819 0 -10 2.75183 1.06602 #262#) 
COMMENT                             #264=(CScratch 
COMMENT                                   (CWidget 0 (3 #23# 0) 1 2 0 0 #166# 28963848 
COMMENT                                    100) (LOGPEN 0 8 8388608) 10 1 5.22538 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #265=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #210# 393219 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "D314E" "@N" 1 
COMMENT                                   15.1159 7.33127 0 -10 2.75183 1.06602 #264#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #266=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -718 100) 
COMMENT                     (CObjectList 
COMMENT                      #267=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -718 100) 
COMMENT                            (CObjectList 
COMMENT                             #268=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #216# 1195852609 
COMMENT                                    100) (LOGPEN 0 0 16724736) 1 1 4.94114 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #269=(CLabel (CWidget 0 (0 0) 1 2 0 0 #213# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Bgl}II (1271)" "@N (@S)" 5 16.3874 
COMMENT                                   3.63043 0 -10 4.2641 0.99165 #268#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #270=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -1143 100) 
COMMENT                            (CObjectList 
COMMENT                             #271=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #216# 28488124 
COMMENT                                    100) (LOGPEN 0 0 16724736) 1 1 6.21445 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #272=(CLabel (CWidget 0 (0 0) 1 2 0 0 #213# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Not}I (57)" "@N (@S)" 5 2.94886 17.0058 
COMMENT                                   0 -10 3.24765 0.99165 #271#)) (CObjectList)) 
COMMENT                      #273=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -632 100) 
COMMENT                            (CObjectList 
COMMENT                             #274=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #216# 27873996 
COMMENT                                    100) (LOGPEN 0 0 16724736) 1 1 2.39138 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #275=(CLabel (CWidget 0 (0 0) 1 2 0 0 #213# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Pvu}I (3702)" "@N (@S)" 5 -12.1936 
COMMENT                                   -11.1908 0 -10 4.09056 0.99165 #274#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #276=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -683 100) 
COMMENT                            (CObjectList 
COMMENT                             #277=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #216# 28209184 
COMMENT                                    100) (LOGPEN 0 0 16724736) 1 1 5.87462 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #278=(CLabel (CWidget 0 (0 0) 1 2 0 0 #213# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Bal}I (381)" "@N (@S)" 5 8.45142 
COMMENT                                   15.6284 0 -10 3.59473 0.99165 #277#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #279=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #6# 0) 1 2 0 0 Nil -446 100) 
COMMENT                            (CObjectList 
COMMENT                             #280=(CScratch 
COMMENT                                   (CWidget 1 (1 #6# 1) 1 2 0 0 #216# 2082602632 
COMMENT                                    100) (LOGPEN 0 0 16724736) 1 1 5.82113 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #281=(CLabel (CWidget 0 (0 0) 1 2 0 0 #213# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Kpn}I (432)" "@N (@S)" 5 9.45891 
COMMENT                                   13.2098 0 -10 3.84264 0.99165 #280#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #282=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #1# 0) 1 2 0 0 Nil -1328 100) 
COMMENT                            (CObjectList 
COMMENT                             #283=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #215# 1094797140 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 5.36068 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #284=(CLabel (CWidget 0 (0 0) 1 2 0 0 #214# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (871)" "@N (@S)" 5 15.0477 
COMMENT                                   9.92345 0 -10 4.58638 0.99165 #283#) 
COMMENT                             #285=(CScratch 
COMMENT                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #215# 25114200 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 4.45238 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #286=(CLabel (CWidget 0 (0 0) 1 2 0 0 #214# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (1737)" "@N (@S)" 5 18.168 
COMMENT                                   -4.57151 0 -10 4.98304 0.99165 #285#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #287=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #2# 0) 1 2 0 0 Nil -1268 100) 
COMMENT                            (CObjectList 
COMMENT                             #288=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #215# 28576040 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 6.26165 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #289=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #214# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Nco}I (12)" "@N (@S)" 5 -1.6826 17.9866 
COMMENT                                   0 -10 3.32203 0.99165 #288#) 
COMMENT                             #290=(CScratch 
COMMENT                                   (CWidget 2 (1 #2# 2) 1 2 0 0 #215# 2081903016 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 4.16185 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #291=(CLabel (CWidget 0 (0 0) 1 2 0 0 #214# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Nco}I (2014)" "@N (@S)" 5 16.1511 
COMMENT                                   -9.14699 0 -10 4.16493 0.99165 #290#) 
COMMENT                             #292=(CScratch 
COMMENT                                   (CWidget 3 (1 #2# 3) 1 2 0 0 #215# 6 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 1.41805 1.9 0.082322 1) 
COMMENT                             #293=(CLabel (CWidget 0 (0 0) 1 2 0 0 #214# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Nco}I (4630)" "@N (@S)" 5 -15.5563 
COMMENT                                   2.48607 0 -10 4.16493 0.99165 #292#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #294=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -673 100) 
COMMENT                     (CObjectList 
COMMENT                      #295=(CLabel (CWidget 0 (15 #24# 0) 1 2 0 0 #186# 0 100) 
COMMENT                            (LOGPEN 0 0 16724787) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 6093" 
COMMENT                            "LMBP 6093" 65535 0.0743738 -2.47913 0 -10 6.84239 
COMMENT                            1.56185 Nil) 
COMMENT                      #296=(CLabel (CWidget 0 (15 #25# 0) 1 2 0 0 #186# 0 100) 
COMMENT                            (LOGPEN 0 0 16724787) 1 
COMMENT                            (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 27-JAN-2014" 
COMMENT                            "Last modified: 27-JAN-2014" 65535 0.495825 -4.21451 
COMMENT                            0 -10 10.9329 1.06602 Nil)) (CObjectList)) 
COMMENT               #297=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -603 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1953068654 
COMMENT       (CStringList) 1952671094 891318895 (CObList)))
FEATURES             Location/Qualifiers
     CDS             64..1269
                     /vntifkey="4"
                     /label=mCASP-1\mutant
     terminator      1271..1362
                     /vntifkey="43"
                     /label=3'\UTR\of\ß-globin,\incl.\polyA
     rep_origin      2021..2096
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      2127..2201
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2127..2201)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(2316..2717)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        4254..4631
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        4637..5978
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     CDS             complement(3258..4118)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     intron          5006..5930
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     misc_feature    1363..1719
                     /vntifkey="21"
                     /label=3'\flanking\region\rabbit\ß-globin
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        complement(1797..1879)
                     /vntifkey="30"
                     /label=lac\promoter
     mutation        367..369
                     /vntifkey="62"
                     /label=D103E
                     /note="WT: GAT"
     mutation        424..426
                     /vntifkey="62"
                     /label=D122E
                     /note="WT: GAT"
     mutation        946..948
                     /vntifkey="62"
                     /label=D296E
                     /note="WT: GAT"
     mutation        1000..1002
                     /vntifkey="62"
                     /label=D314E
                     /note="WT: GAT"
BASE COUNT     1383 a      1502 c      1606 g      1490 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctgctgaca agatcctgag ggcaaagagg aagcaattta tcaactcagt gagtataggg 
      121 acaataaatg gattgttgga tgaactttta gagaagagag tgctgaatca ggaagaaatg 
      181 gataaaataa aacttgcaaa cattactgct atggacaagg cacgggacct atgtgatcat 
      241 gtctctaaaa aagggcccca ggcaagccaa atctttatca cttacatttg taatgaagac 
      301 tgctacctgg caggaattct ggagcttcaa tcagctccat cagctgaaac atttgttgct 
      361 acagaagaat ctaaaggtgg ccatccttca tcctcagaaa caaaggaaga acagaacaaa 
      421 gaagagggta cctttccagg actgactggg accctcaagt tttgcccttt agaaaaagcc 
      481 cagaagttat ggaaagaaaa tccttcagag atttatccaa taatgaatac aaccactcgt 
      541 acacgtcttg ccctcattat ctgcaacaca gagtttcaac atctttctcc gagggttgga 
      601 gctcaagttg acctcagaga aatgaagttg ctgctggagg atctggggta taccgtgaaa 
      661 gtgaaagaaa atctcacagc tctggagatg gtgaaagagg tgaaagaatt tgctgcctgc 
      721 ccagagcaca agacttctga cagtactttc cttgtattca tgtctcatgg tatccaggag 
      781 ggaatatgtg ggaccacata ctctaatgaa gtttcagata ttttaaaggt tgacacaatc 
      841 tttcagatga tgaacacttt gaagtgccca agcttgaaag acaagcccaa ggtgatcatt 
      901 attcaggcat gccgtggaga gaaacaagga gtggtgttgt taaaagaatc agtaagagac 
      961 tctgaagagg atttcttaac ggatgcaatt tttgaagatg aaggcattaa gaaggcccat 
     1021 atagagaaag attttattgc tttctgctct tcaacaccag ataatgtgtc ttggagacat 
     1081 cctgtcaggg gctcactttt cattgagtca ctcatcaaac acatgaaaga atatgcctgg 
     1141 tcttgtgact tggaggacat tttcagaaag gttcgatttt catttgaaca accagaattt 
     1201 aggctacaga tgcccactgc tgatagggtg accctgacaa aacgtttcta cctcttcccg 
     1261 ggacattaaa gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga 
     1321 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt 
     1381 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga 
     1441 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg 
     1501 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag 
     1561 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt 
     1621 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact 
     1681 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct 
     1741 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 
     1801 acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact 
     1861 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg 
     1921 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 
     1981 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 
     2041 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 
     2101 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat 
     2161 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 
     2221 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg 
     2281 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     2341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     2401 acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
     2461 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     2521 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     2581 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     2641 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 
     2701 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     2761 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     2821 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     2881 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 
     2941 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     3001 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
     3061 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     3121 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     3181 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     3241 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     3301 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     3361 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     3421 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     3481 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     3541 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 
     3601 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     3661 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     3721 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     3781 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     3841 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 
     3901 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     3961 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     4021 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     4081 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     4141 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     4201 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg 
     4261 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 
     4321 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 
     4381 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 
     4441 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 
     4501 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 
     4561 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 
     4621 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 
     4681 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 
     4741 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc 
     4801 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 
     4861 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt 
     4921 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct 
     4981 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 
     5041 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga 
     5101 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt 
     5161 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg 
     5221 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc 
     5281 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg 
     5341 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc 
     5401 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg 
     5461 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc 
     5521 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc 
     5581 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 
     5641 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta 
     5701 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc 
     5761 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg 
     5821 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 
     5881 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 
     5941 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a 
//
