ID pCAGGS-E-mCASP-1-4DE standard; circular DNA; ; 5981 BP. DE LMBP 6093. CC Accession number: LMBP 6093 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 27-JAN-2014 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 64..1269 FT /vntifkey="4" FT /label=mCASP-1\mutant FT terminator 1271..1362 FT /vntifkey="43" FT /label=3'\UTR\of\ß-globin,\incl.\polyA FT rep_origin 2021..2096 FT /vntifkey="33" FT /label=SV40\ori FT terminator 2127..2201 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2127..2201) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(2316..2717) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 4254..4631 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter 4637..5978 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT CDS complement(3258..4118) FT /vntifkey="4" FT /label=ampicillin\resistance FT intron 5006..5930 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid intron" FT misc_feature 1363..1719 FT /vntifkey="21" FT /label=3'\flanking\region\rabbit\ß-globin FT misc_marker 16..54 FT /vntifkey="22" FT /label=E-tag FT promoter complement(1797..1879) FT /vntifkey="30" FT /label=lac\promoter FT mutation 367..369 FT /vntifkey="62" FT /label=D103E FT /note="WT: GAT" FT mutation 424..426 FT /vntifkey="62" FT /label=D122E FT /note="WT: GAT" FT mutation 946..948 FT /vntifkey="62" FT /label=D296E FT /note="WT: GAT" FT mutation 1000..1002 FT /vntifkey="62" FT /label=D314E FT /note="WT: GAT" SQ Sequence 5981 BP; 1383 A; 1502 C; 1606 G; 1490 t; ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 60 gctgctgaca agatcctgag ggcaaagagg aagcaattta tcaactcagt gagtataggg 120 acaataaatg gattgttgga tgaactttta gagaagagag tgctgaatca ggaagaaatg 180 gataaaataa aacttgcaaa cattactgct atggacaagg cacgggacct atgtgatcat 240 gtctctaaaa aagggcccca ggcaagccaa atctttatca cttacatttg taatgaagac 300 tgctacctgg caggaattct ggagcttcaa tcagctccat cagctgaaac atttgttgct 360 acagaagaat ctaaaggtgg ccatccttca tcctcagaaa caaaggaaga acagaacaaa 420 gaagagggta cctttccagg actgactggg accctcaagt tttgcccttt agaaaaagcc 480 cagaagttat ggaaagaaaa tccttcagag atttatccaa taatgaatac aaccactcgt 540 acacgtcttg ccctcattat ctgcaacaca gagtttcaac atctttctcc gagggttgga 600 gctcaagttg acctcagaga aatgaagttg ctgctggagg atctggggta taccgtgaaa 660 gtgaaagaaa atctcacagc tctggagatg gtgaaagagg tgaaagaatt tgctgcctgc 720 ccagagcaca agacttctga cagtactttc cttgtattca tgtctcatgg tatccaggag 780 ggaatatgtg ggaccacata ctctaatgaa gtttcagata ttttaaaggt tgacacaatc 840 tttcagatga tgaacacttt gaagtgccca agcttgaaag acaagcccaa ggtgatcatt 900 attcaggcat gccgtggaga gaaacaagga gtggtgttgt taaaagaatc agtaagagac 960 tctgaagagg atttcttaac ggatgcaatt tttgaagatg aaggcattaa gaaggcccat 1020 atagagaaag attttattgc tttctgctct tcaacaccag ataatgtgtc ttggagacat 1080 cctgtcaggg gctcactttt cattgagtca ctcatcaaac acatgaaaga atatgcctgg 1140 tcttgtgact tggaggacat tttcagaaag gttcgatttt catttgaaca accagaattt 1200 aggctacaga tgcccactgc tgatagggtg accctgacaa aacgtttcta cctcttcccg 1260 ggacattaaa gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga 1320 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt 1380 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga 1440 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg 1500 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag 1560 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt 1620 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact 1680 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct 1740 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 1800 acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact 1860 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg 1920 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 1980 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 2040 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 2100 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat 2160 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 2220 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg 2280 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 2340 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 2400 acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 2460 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 2520 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 2580 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 2640 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 2700 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 2760 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 2820 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 2880 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 2940 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3000 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3060 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 3120 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 3180 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 3240 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 3300 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 3360 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 3420 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 3480 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 3540 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 3600 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 3660 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 3720 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 3780 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 3840 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 3900 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 3960 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 4020 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 4080 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 4140 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 4200 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg 4260 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 4320 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 4380 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 4440 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 4500 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 4560 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 4620 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 4680 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 4740 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc 4800 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 4860 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt 4920 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct 4980 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 5040 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga 5100 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt 5160 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg 5220 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc 5280 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg 5340 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc 5400 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg 5460 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc 5520 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc 5580 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 5640 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta 5700 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc 5760 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg 5820 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 5880 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 5940 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a 5981 //