LOCUS pCD-F-HLH-MALT-C464A-MycHis 8439 bp DNA circular 8-OCT-2013 DEFINITION LMBP 6101. SOURCE ORGANISM COMMENT Accession number: LMBP 6101 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) Last modified: 8-OCT-2013 COMMENT VNTDATE|675337782| COMMENT VNTNAME|pCD-F-HLH-MALT-C464A-MycHis| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pCD-F-HLH-MALT-C464A-MycHis" 0 8439 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 COMMENT 0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) COMMENT (CWordArray) COMMENT (CStringList "ApaI" "BamHI" "BssHII" "HindIII" "XmaI" "XmnI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 8439 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 7294 0 0 "") COMMENT #1=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 4310 0 0 "") COMMENT #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 4266 0 6307 0 0 "") COMMENT #3=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 8438 0 0 "") COMMENT #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 5 3031 0 4717 0 5170 0 COMMENT 6084 0 8088 0 0 "") COMMENT #5=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 2 585 0 6207 0 0 "")) COMMENT (CObList COMMENT #6=(CFSignal (CObList) "hMALT1" 4 0 0 4817 7288 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4817..7288") COMMENT #7=(CFSignal (CObList) "HLH dimerization domain" 4 0 0 4322 4801 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4322..4801") COMMENT #8=(CFSignal (CObList) "Myc epitope" 22 0 0 7298 7327 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "7298..7327") COMMENT #9=(CFSignal (CObList) "His-tag" 22 0 0 7343 7360 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "7343..7360") COMMENT #10=(CFSignal (CObList) "BGH polyA" 43 0 0 7389 7613 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "7389..7613") COMMENT #11=(CFSignal (CObList) "f1 ori" 33 0 0 7659 8087 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "7659..8087") COMMENT #12=(CFSignal (CObList) "SV40 early promoter" 29 0 0 8092 8374 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "8092..8374") COMMENT #13=(CFSignal (CObList) "SV40 ori" 33 0 0 8331 8406 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "8331..8406") COMMENT #14=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 59 853 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "59..853") COMMENT #15=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1027 1101 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1027..1101") COMMENT #16=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1027 1101 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(1027..1101)") COMMENT #17=(CFSignal (CObList) "lac promoter" 30 0 1 1240 1323 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1240..1323)") COMMENT #18=(CFSignal (CObList) "pMB1 ori" 33 0 1 1415 1817 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1415..1817)") COMMENT #19=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2358 3218 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2358..3218)") COMMENT #20=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 3589 4172 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "3589..4172") COMMENT #21=(CFSignal (CObList) "T7 promoter" 30 0 0 4217 4233 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4217..4233") COMMENT #22=(CFSignal (CObList) "FLAG epitope" 22 0 0 4277 4300 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4277..4300") COMMENT #23=(CFSignal (CObList) "C464A" 62 0 0 6206 6208 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "6206..6208")) (CObList) (CObList) (CObList) COMMENT (CObList) COMMENT (CObList COMMENT #24=(CFSignal (CObList) "Last modified: 8-OCT-2013" 2 0 0 0 0 0 COMMENT (CStringList) (CStringList) 1 1 0 0 "") COMMENT #25=(CFSignal (CObList) "LMBP 6101" 2 0 0 0 0 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "")) (CObList) COMMENT (CTextView 0 COMMENT #26=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #27=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) COMMENT "pCD-F-HLH-MALT-C464A-MycHis" 2) 5 "" 0 4) COMMENT #28=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pCD-F-HLH-MALT-C464A-MycHis'" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Currently local object. 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65535 -0.421451 -2.72704 0 -10 6.84239 COMMENT 1.56185 Nil)) (CObjectList)) COMMENT #296=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -817 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers CDS 4817..7288 /vntifkey="4" /label=hMALT1 CDS 4322..4801 /vntifkey="4" /label=HLH\dimerization\domain misc_marker 7298..7327 /vntifkey="22" /label=Myc\epitope misc_marker 7343..7360 /vntifkey="22" /label=His-tag terminator 7389..7613 /vntifkey="43" /label=BGH\polyA rep_origin 7659..8087 /vntifkey="33" /label=f1\ori promoter 8092..8374 /vntifkey="29" /label=SV40\early\promoter rep_origin 8331..8406 /vntifkey="33" /label=SV40\ori CDS 59..853 /vntifkey="4" /label=Tn5\neomycin\resistance terminator 1027..1101 /vntifkey="43" /label=SV40\polyA\early terminator complement(1027..1101) /vntifkey="43" /label=SV40\polyA\late promoter complement(1240..1323) /vntifkey="30" /label=lac\promoter rep_origin complement(1415..1817) /vntifkey="33" /label=pMB1\ori CDS complement(2358..3218) /vntifkey="4" /label=ampicillin\resistance promoter 3589..4172 /vntifkey="29" /label=hCMV-IE\promoter\and\enhancer promoter 4217..4233 /vntifkey="30" /label=T7\promoter misc_marker 4277..4300 /vntifkey="22" /label=FLAG\epitope mutation 6206..6208 /vntifkey="62" /label=C464A /note="WT: TGT" BASE COUNT 2092 a 2085 c 2102 g 2160 t ORIGIN 1 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 61 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 121 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 181 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 241 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 301 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 361 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 421 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 481 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 541 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 601 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 661 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 721 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 781 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 841 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 901 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 961 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 1021 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 1081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 1141 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 1201 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 1261 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 1321 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 1381 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 1441 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 1501 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 1561 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 1621 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 1681 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 1741 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 1801 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 1861 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 1921 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 1981 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 2041 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 2101 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 2161 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 2221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 2281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 2341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 2461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 2581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 2641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 2701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 2761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 2821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 2881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 2941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 3001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 3061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 3121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 3181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 3241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 3301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga 3361 tcgggagatc tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat 3421 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 3481 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 3541 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 3601 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 3661 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 3721 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 3781 gtcaatgggt ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 3841 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 3901 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 3961 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 4021 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 4081 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 4141 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt 4201 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagcgtttaa 4261 acttaagctt accatggatt acaaggacga tgacgataag gtacctaagg atcctgccac 4321 catggcgctg ttgggggacc cgctacaggc cctgccgccc tcggccgccc ccacggggcc 4381 gctgctcgcc cctccggccg gcgcgaccct caaccgcctg cgggagccgc tgctgcggag 4441 gctcagctac gcttcctcga cgccacttca tgttccagtg cctcgagcgc tcaggatgga 4501 ggaagactcg atccgcctgc ctgcgcacct gcgcttgcag ccaatttact ggagcaggga 4561 tgacgtagcc cagtggctca agtgggctga aaatgagttt tctttaaggc caattgacag 4621 caacacgttt gaaatgaatg gcaaagctct cctgctgctg accaaagagg actttcgcta 4681 tcgatctcct cattcaggtg atgtgctcta tgaactcctt cagcatattc tgaagcagag 4741 gaaacctcgg attctttttt caccattctt ccaccctgga aactctatac acacacagcc 4801 ggttgatcct gccaccatgg cgctgttggg ggacccgcta caggccctgc cgccctcggc 4861 cgcccccacg gggccgctgc tcgcccctcc ggccggcgcg accctcaacc gcctgcggga 4921 gccgctgctg cggaggctca gcgagctcct ggatcaggcg cccgagggcc ggggctggag 4981 gagactggcg gagctggcgg ggagtcgcgg gcgcctccgc ctcagttgcc tagacctgga 5041 gcagtgttct cttaaggtac tggagcctga aggaagcccc agcctgtgtc tgctgaagtt 5101 aatgggtgaa aaaggttgca cagtcacaga attgagtgat ttcctgcagg ctatggaaca 5161 cactgaagtt cttcagcttc tcagcccccc aggaataaag attactgtaa acccagagtc 5221 aaaggcagtc ttggctggac agtttgtgaa actgtgttgc cgggcaactg gacatccttt 5281 tgttcaatat cagtggttca aaatgaataa agagattcca aatggaaata catcagagct 5341 tatttttaat gcagtgcatg taaaagatgc aggcttttat gtctgtcgag ttaataacaa 5401 tttcaccttt gaattcagcc agtggtcaca gctggatgtt tgcgacatcc cagagagctt 5461 ccagagaagt gttgatggcg tctctgaatc caagttgcaa atctgtgttg aaccaacttc 5521 ccaaaagctg atgccaggca gcacattggt tttacagtgt gttgctgttg gaagccctat 5581 tcctcactac cagtggttca aaaatgaatt accattaaca catgagacca aaaagctata 5641 catggtgcct tatgtggatt tggaacacca aggaacctac tggtgtcatg tatataatga 5701 tcgagacagt caagatagca agaaggtaga aatcatcata ggaagaacag atgaggcagt 5761 ggagtgcact gaagatgaat taaataatct tggtcatcct gataataaag agcaaacaac 5821 tgaccagcct ttggcgaagg acaaggttgc ccttttgata ggaaatatga attaccggga 5881 gcaccccaag ctcaaagctc ctttggtgga tgtgtacgaa ttgactaact tactgagaca 5941 gctggacttc aaagtggttt cactgttgga tcttactgaa tatgagatgc gtaatgctgt 6001 ggatgagttt ttactccttt tagacaaggg agtatatggg ttattatatt atgcaggaca 6061 tggttatgaa aattttggga acagcttcat ggtccccgtt gatgctccaa atccatatag 6121 gtctgaaaat tgtctgtgtg tacaaaatat actgaaattg atgcaagaaa aagaaactgg 6181 acttaatgtg ttcttattgg atatggcgcg caaaagaaat gactacgatg ataccattcc 6241 aatcttggat gcactaaaag tcaccgccaa tattgtgttt ggatatgcca cgtgtcaagg 6301 agctgaagct tttgaaatcc agcattctgg attggcaaat ggaatcttta tgaaattttt 6361 aaaagacaga ttattagaag ataagaaaat cactgtgtta ctggatgaag ttgcagaaga 6421 tatgggtaag tgtcacctta ccaaaggcaa acaggctcta gagattcgaa gtagtttatc 6481 tgagaagaga gcacttactg atccaataca gggaacagaa tattctgctg aatctcttgt 6541 gcggaatcta cagtgggcca aggctcatga acttccagaa agtatgtgtc ttaagtttga 6601 ctgtggtgtt cagattcaat taggatttgc agctgagttt tccaatgtca tgatcatcta 6661 tacaagtata gtttacaaac caccggagat aataatgtgt gatgcctacg ttactgattt 6721 tccacttgat ctagatattg atccaaaaga tgcaaataaa ggcacgcctg aagaaactgg 6781 cagctacttg gtatcaaagg atcttcccaa gcattgcctc tataccagac tcagttcact 6841 gcaaaaatta aaggaacatc tagtcttcac agtatgttta tcatatcagt actcaggatt 6901 ggaagatact gtagaggaca agcaggaagt gaatgttggg aaacctctca ttgctaaatt 6961 agacatgcat cgaggtttgg gaaggaagac ttgctttcaa acttgtctta tgtctaatgg 7021 tccttaccag agttctgcag ccacctcagg aggagcaggg cattatcact cattgcaaga 7081 cccattccat ggtgtttacc attcacatcc tggtaatcca agtaatgtta caccagcaga 7141 tagctgtcat tgcagccgga ctccagatgc atttatttca agtttcgctc accatgcttc 7201 atgtcatttt agtagaagta atgtgccagt agagacaact gatgaaatac catttagttt 7261 ctctgacagg ctcagaattt ctgaaaaagg gcccttcgaa caaaaactca tctcagaaga 7321 ggatctgaat atgcataccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc 7381 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 7441 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 7501 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 7561 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 7621 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 7681 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 7741 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 7801 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 7861 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 7921 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 7981 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc 8041 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 8101 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag 8161 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 8221 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 8281 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 8341 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 8401 aggctttttt ggaggcctag gcttttgcaa aaagctccc //