LOCUS       pCD-F-hCYLD-N           6435 bp    DNA     circular     14-SEP-2011
DEFINITION  LMBP 6113.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6113
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 14-SEP-2011
            
            
COMMENT     VNTDATE|601215101|
COMMENT     VNTNAME|pCD-F-hCYLD-N|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDBMol 0 0 1 4469 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 2005 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 951 0 0 "")) 
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COMMENT            (CStringList) 1 1 1 1 "2782..3107") 
COMMENT        #3=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 3140 3934 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3143..3937") 
COMMENT        #4=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5439 6299 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "pMB1 ori" 33 0 1 4496 4898 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4108 4182 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4108 4182 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 235 818 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "FLAG epitope" 22 0 0 923 946 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "SV40 ori" 33 0 0 2973 3048 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "hCYLD fragment" 4 0 0 953 1999 0 (CStringList) 
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COMMENT                          "DNA 'pCD-F-hCYLD-N'" 1) 
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COMMENT                          "Currently local object. Original author: Peter Marynen" 
COMMENT                          1) 
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COMMENT                          "Created: 09/14/11 12:11" 1) 
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COMMENT                          "length: 6435 bp" 1) 
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FEATURES             Location/Qualifiers
     promoter        2734..3016
                     /vntifkey="29"
                     /label=SV40
     CDS             3140..3934
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     CDS             953..1999
                     /vntifkey="4"
                     /label=hCYLD\fragment
     CDS             complement(5439..6299)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(4496..4898)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      4108..4182
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4108..4182)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     923..946
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2031..2255
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      2301..2729
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      2973..3048
                     /vntifkey="33"
                     /label=SV40\ori
BASE COUNT     1572 a      1573 c      1640 g      1650 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac caatgagttc 
      961 aggcttatgg agccaagaaa aagtcacttc accctactgg gaagagcgga ttttttactt 
     1021 gcttcttcaa gaatgcagcg ttacagacaa acaaacacaa aagctcctta aagtaccgaa 
     1081 gggaagtata ggacagtata ttcaagatcg ttctgtgggg cattcaagga ttccttctgc 
     1141 aaaaggcaag aaaaatcaga ttggattaaa aattctagag caacctcatg cagttctctt 
     1201 tgttgatgaa aaggatgttg tagagataaa tgaaaagttc acagagttac ttttggcaat 
     1261 taccaattgt gaggagaggt tcagcctgtt taaaaacaga aacagactaa gtaaaggcct 
     1321 ccaaatagac gtgggctgtc ctgtgaaagt acagctgaga tctggggaag aaaaatttcc 
     1381 tggagttgta cgcttcagag gacccctgtt agcagagagg acagtctccg gaatattctt 
     1441 tggagttgaa ttgctggaag aaggtcgtgg tcaaggtttc actgacgggg tgtaccaagg 
     1501 gaaacagctt tttcagtgtg atgaagattg tggcgtgttt gttgcattgg acaagctaga 
     1561 actcatagaa gatgatgaca ctgcattgga aagtgattac gcaggtcctg gggacacaat 
     1621 gcaggtcgaa cttcctcctt tggaaataaa ctccagagtt tctttgaagg ttggagaaac 
     1681 aatagaatct ggaacagtta tattctgtga tgttttgcca ggaaaagaaa gcttaggata 
     1741 ttttgttggt gtggacatgg ataaccctat tggcaactgg gatggaagat ttgatggagt 
     1801 gcagctttgt agttttgcgt gtgttgaaag tacaattcta ttgcacatca atgatatcat 
     1861 cccagcttta tcagagagtg tgacgcagga aaggaggcct cccaaacttg cctttatgtc 
     1921 aagaggtgtt ggggacaaag gttcatccag tcataataaa ccaaaggcta caggatctac 
     1981 ctcagaccct ggaaattagg ggcccgttta aacccgctga tcagcctcga ctgtgccttc 
     2041 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
     2101 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
     2161 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
     2221 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 
     2281 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     2341 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     2401 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat 
     2461 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     2521 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     2581 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     2641 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga 
     2701 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 
     2761 ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc 
     2821 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 
     2881 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 
     2941 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 
     3001 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 
     3061 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac 
     3121 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 
     3181 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 
     3241 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 
     3301 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 
     3361 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 
     3421 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 
     3481 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 
     3541 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 
     3601 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 
     3661 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 
     3721 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 
     3781 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 
     3841 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 
     3901 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 
     3961 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 
     4021 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 
     4081 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 
     4141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 
     4201 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 
     4261 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 
     4321 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 
     4381 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     4441 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 
     4501 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     4561 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     4621 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     4681 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     4741 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     4801 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     4861 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     4921 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     4981 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     5041 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     5101 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     5161 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     5221 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     5281 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     5341 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     5401 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     5461 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     5521 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     5581 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     5641 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     5701 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     5761 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     5821 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     5881 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     5941 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     6001 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     6061 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     6121 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     6181 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     6241 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     6301 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     6361 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     6421 gtgccacctg acgtc 
//
