ID pCAGGS-E-SopE-G168V standard; circular DNA; ; 5612 BP. DE LMBP 6116. CC Accession number: LMBP 6116 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 1-DEC-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 16..54 FT /vntifkey="22" FT /label=E-tag FT intron 4637..5561 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter intron" FT promoter 4268..5609 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT promoter 3885..4262 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT CDS complement(2889..3749) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(1947..2348) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT terminator complement(1758..1832) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 1758..1832 FT /vntifkey="43" FT /label=SV40\polyA\early FT rep_origin 1652..1727 FT /vntifkey="33" FT /label=SV40\ori FT promoter complement(1428..1510) FT /vntifkey="30" FT /label=lac\promoter FT misc_feature 994..1350 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT terminator 902..993 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT CDS 64..786 FT /vntifkey="4" FT /label=sopE\mutant FT mutation 565..567 FT /vntifkey="62" FT /label=G168V FT /note="wt: GGG" SQ Sequence 5612 BP; 1275 A; 1458 C; 1498 G; 1381 t; ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 60 gctgtgacaa aaataacttt atctccccag aattttagaa tccaaaaaca ggaaaccaca 120 ctactaaaag aaaaatcaac cgagaaaaat tctttagcaa aaagtattct cgcagtaaaa 180 aatcacttca tcgaattaag gtcaaaatta tcggaacgtt ttatttcgca taagaacact 240 gagtcttctg caacacactt tcaccgagga agcgcatctg agggccgggc agtgttgaca 300 aataaagtcg ttaaagattt tatgcttcaa acgctcaatg atatagatat tagaggtagt 360 gcgagtaaag accccgcata cgccagccag acccgtgaag ctatactatc ggcagtttac 420 agcaagaata aagatcagtg ttgtaatttg ctcatcagca aagggatcaa catagcgcct 480 tttcttcagg aaattggcga agcagcgaaa aatgcaggtc tgcccggaac aaccaaaaat 540 gacgttttta cgccaagcgg cgcagtggcc aatcctttta taactccgtt gatttcatca 600 gcaaacagta agtatccacg tatgtttatc aatcaacatc agcaggcatc ctttaaaatc 660 tatgcggaga agatcattat gacagaagtt gcaccactgt ttaatgagtg tgctatgccg 720 actccacagc aattccaact gatactagaa aacattgcta ataaatatat acaaaacact 780 ccctaacccg ggacattaaa cgcgtgggta ccggatccgg caattcactc ctcaggtgca 840 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg 900 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac 960 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct 1020 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt 1080 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag 1140 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt 1200 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct 1260 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt 1320 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat 1380 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 1440 gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat 1500 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat 1560 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 1620 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 1680 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 1740 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata 1800 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 1860 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc 1920 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 1980 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 2040 cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 2100 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 2160 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 2220 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 2280 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 2340 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 2400 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 2460 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2520 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 2580 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2640 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2700 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 2760 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 2820 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 2880 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 2940 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 3000 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 3060 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 3120 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 3180 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 3240 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 3300 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 3360 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 3420 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 3480 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 3540 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 3600 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 3660 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 3720 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 3780 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 3840 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac 3900 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 3960 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 4020 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 4080 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 4140 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 4200 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 4260 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc 4320 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg 4380 gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa 4440 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg 4500 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg 4560 ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac 4620 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg 4680 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg 4740 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg 4800 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg 4860 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg 4920 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc 4980 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc 5040 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt 5100 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg 5160 ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg 5220 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt 5280 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg 5340 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc 5400 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc 5460 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag 5520 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg 5580 ttattgtgct gtctcatcat tttggcaaag aa 5612 //