LOCUS       pCAGGS-E-mRip2          6395 bp    DNA     circular     12-JAN-2010
DEFINITION  LMBP 6121.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6121
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 12-JAN-2010
            
            
            
            
            
            
COMMENT     VNTDATE|542992236|
COMMENT     VNTNAME|pCAGGS-E-mRip2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-mRip2" 0 6395 
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COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "BglII" "EcoRI" "NotI" "SpeI" "Alw44I" "HindIII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6395 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 4 1100 0 1377 0 2335 0 
COMMENT            2672 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BglII" "agatct" 2 0 1 1685 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 4683 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 2 3167 0 4413 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 1675 0 2151 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3672 4532 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3672..4532)") 
COMMENT        #9=(CFSignal (CObList) "pMB1 ori" 33 0 1 2730 3131 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2730..3131)") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2541 2615 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2541..2615)") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2541 2615 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2541..2615") 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 2435 2510 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2435..2510") 
COMMENT        #13=(CFSignal (CObList) "lac promoter" 30 0 1 2211 2293 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2211..2293)") 
COMMENT        #14=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
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COMMENT        #18=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
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COMMENT        #19=(CFSignal (CObList) "mRIP2" 4 0 0 64 1683 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
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COMMENT                          "Created: 01/12/10 03:10" 1) 
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COMMENT                          "length: 6395 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 12-JAN-2010" 1))) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     intron          5420..6344
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3672..4532)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2730..3131)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2541..2615)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2541..2615
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      2435..2510
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(2211..2293)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1685..1776
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        5051..6392
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4668..5045
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1777..2133
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..1683
                     /vntifkey="4"
                     /label=mRIP2
BASE COUNT     1464 a      1663 c      1687 g      1581 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctatgaacg gggacgccat ctgcagcgcg ctacccccca tcccgtacca caagctcgcc 
      121 gacctgcact acctcagccg gggcgcctct ggcacggtct cgtcggcacg acacgcagac 
      181 tggcgcgtgc gggtggcagt gaagcaccta cacatccaca cgccgctgct cgacagtgaa 
      241 agaaatgata tcttacgaga agccgaaata ttacataaag caagatttag ttacattctt 
      301 ccaattttgg gaatttgcaa tgagcctgaa tttttgggca tagttactga atacatgcca 
      361 aatggatcat taaatgagct tctacacaga aaaactgaat atcctgatat tgcatggcca 
      421 ttgagattcc gcatcctgca tgaaattgct cttggtgtaa attatctgca caatatgaac 
      481 ccccctctgc ttcatcatga cctaaagact cagaatatct tgttggataa tgaatttcat 
      541 gttaagattg cagattttgg tttatcaaaa tggcgcatga tgtcactctc tcaatcacga 
      601 agttacaaat ctgcacccga aggaggaaca atcatctata tgccacctga gaactatgag 
      661 ccaggacaga aatcaagggc cagtgtgaag catgatatat acagctatgc agttatcatg 
      721 tgggaagtgt tatccagaaa acagcctttt gaagaggtca ccaatccttt gcagatcatg 
      781 tacagtgtgt cccagggaca tcgacctgac accagtgagg agaatttgcc atttgatata 
      841 cctcatcgag gtctcatgat ctctctaata cagagtggat gggcgcaaaa cccagatgaa 
      901 agaccatcct ttttgaaatg cttaatagaa cttgaaccag ttctgagaac atttgaagac 
      961 ataactttcc ttgaggctgt tattcagcta aagaaagcaa agatacagag ttcctcaagt 
     1021 actattcact tatgtgataa gaaaatggat ttatcgctaa acatacctgc aaatcatccc 
     1081 ccacaggagg aatcgtgtgg atcctctctg ctctctagaa acactggttc tcctggaccc 
     1141 tccagatccc tatcagctcc tcaagacaag ggatttttat ctggagcacc tcaagactgc 
     1201 tcttcgttga aggcacacca ctgtcctgga aaccacagct gggatggtat cgtttctgta 
     1261 ccaccagggg cagccttctg tgatcgcagg gcctcctcgt gttccttggc tgtaataagt 
     1321 ccattcttgg ttgagaaagg ttcagagcgc ccaccaattg gtatagccca acagtggatc 
     1381 cagagtaaga gggaagccat tgtgagccag atgactgaag cctgccttaa ccaatcgctg 
     1441 gacgcccttc tgtctcgtga cctgatcatg aaggaggact atgaactcat tagcaccaaa 
     1501 cctacaagga cctcaaaagt cagacagctg ctagatacgt ctgacatcca aggagaagag 
     1561 tttgccaaag ttgtagtgca gaagttgaaa gataacaagc agctggggct gcagccttac 
     1621 ccagaagtac ctgtgctttc caaagcaccg ccttcaaatt tccctcagaa taaaagcttg 
     1681 tgaagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct 
     1741 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg 
     1801 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt 
     1861 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata 
     1921 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc 
     1981 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc 
     2041 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc 
     2101 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt 
     2161 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     2221 tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct aactcacatt 
     2281 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg 
     2341 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 
     2401 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 
     2461 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 
     2521 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca 
     2581 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     2641 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg 
     2701 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     2761 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     2821 atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     2881 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     2941 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     3001 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     3061 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     3121 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     3181 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     3241 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     3301 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 
     3361 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     3421 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     3481 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     3541 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     3601 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     3661 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     3721 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     3781 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     3841 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     3901 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     3961 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 
     4021 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     4081 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     4141 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     4201 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     4261 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 
     4321 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     4381 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     4441 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     4501 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 
     4561 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 
     4621 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt 
     4681 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 
     4741 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 
     4801 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 
     4861 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 
     4921 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 
     4981 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 
     5041 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 
     5101 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 
     5161 gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 
     5221 gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 
     5281 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg 
     5341 ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 
     5401 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta 
     5461 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct 
     5521 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt 
     5581 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc 
     5641 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg 
     5701 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg 
     5761 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt 
     5821 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc 
     5881 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc 
     5941 cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg 
     6001 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt 
     6061 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc 
     6121 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt 
     6181 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct 
     6241 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta 
     6301 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc 
     6361 tggttattgt gctgtctcat cattttggca aagaa 
//
