ID pAS2-mRIP1-ID-DD standard; circular DNA; ; 9444 BP. DE LMBP 6212. CC Accession number: LMBP 6212 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 9-APR-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(8454..9314) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(7511..7913) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(7336..7419) FT /vntifkey="30" FT /label=lac\promoter FT terminator 7082..7183 FT /vntifkey="43" FT /label=ADH1\terminator FT misc_marker 5957..5983 FT /vntifkey="22" FT /label=HA\epitope FT promoter 5075..5478 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS complement(3855..4304) FT /vntifkey="4" FT /label=CYH2 FT terminator complement(3312..3586) FT /vntifkey="43" FT /label=CYC1\terminator FT rep_origin complement(2661..3116) FT /vntifkey="33" FT /label=f1\ori FT CDS 1884..2558 FT /vntifkey="4" FT /label=TRP1 FT CDS 5501..5941 FT /vntifkey="4" FT /label=GALbd FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT CDS 5987..7048 FT /vntifkey="4" FT /label=mRIP1-ID-DD SQ Sequence 9444 BP; 2687 A; 1998 C; 2124 G; 2635 t; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2220 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2280 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2340 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2400 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2460 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2520 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2580 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2640 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2700 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2760 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2820 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2880 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2940 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3000 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3060 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3120 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3180 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3240 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3300 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3360 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3420 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3480 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3540 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3600 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3660 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3720 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3780 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3840 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3900 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3960 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4020 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4080 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4140 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4200 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4260 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4320 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4380 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4440 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4500 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4560 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4620 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4680 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4740 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4800 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4860 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4920 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4980 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5040 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5100 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5160 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5220 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 5280 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5340 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5400 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5460 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5520 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5580 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5640 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5700 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5760 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5820 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5880 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5940 gccggaattc atggcttacc catacgatgt tccagattac gctagcatgt taaagaaaga 6000 gtatccagat caaagcccag tgctgcagag aatgttttca ctgcagcatg actgtgtacc 6060 cttacctccg agcaggtcaa attcagaaca acctggatcg ctgcacagtt cccaggggct 6120 ccagatgggt cctgtggagg agtcctggtt ttcttcctcc ccagagtacc cacaggacga 6180 gaatgatcgc agtgtgcagg ctaagctgca agaggaagcc agctatcatg cttttggaat 6240 atttgcagag aaacagacaa aaccgcagcc aaggcagaat gaggcttaca acagagagga 6300 ggaaaggaaa cgaagggtct ctcatgaccc ctttgcacag cagagagctc gtgagaatat 6360 taagagtgca ggagcaagag gtcattctga tcccagcaca acgagtcgtg gaattgcagt 6420 gcaacagctg tcatggccag ccacccaaac agtttggaac aatggattgt ataatcagca 6480 tggatttgga actacaggta caggagtttg gtatccgcca aatctaagcc aaatgtatag 6540 tacttataaa actccagtgc cagagaccaa cataccggga agcacaccca ccatgccata 6600 cttctctggg ccagtagcag atgacctcat aaaatatact atattcaata gttctggtat 6660 tcagattgga aaccacaatt atatggatgt tggactgaat tcacaaccac caaacaatac 6720 ttgcaaagaa gagtcgactt ccagacacca agccatcttt gataacacca ctagtctgac 6780 tgatgaacac ctgaacccta tcagggaaaa cctgggaagg cagtggaaaa actgtgcccg 6840 caagctgggc ttcactgagt ctcagatcga tgaaatcgac catgactatg aaagagatgg 6900 actgaaagag aaagtttacc aaatgcttca gaagtggctg atgcgggaag gcaccaaagg 6960 ggccacagtg ggaaagttgg cccaggcact tcaccaatgt tgcaggatag acctgctgaa 7020 ccacttgatt cgtgccagcc agagctaagg atccgtcgac ctgcagccaa gctaattccg 7080 ggcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt 7140 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttgttct tgagtaactc 7200 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac 7260 ctctaccggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 7320 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 7380 gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 7440 ggaaacctgt cgtgccagct ggattaatga atcggccaac gcgcggggag aggcggtttg 7500 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 7560 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 7620 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 7680 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 7740 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 7800 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 7860 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 7920 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 7980 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 8040 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 8100 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 8160 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 8220 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 8280 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 8340 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 8400 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 8460 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 8520 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 8580 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 8640 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 8700 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 8760 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 8820 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 8880 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 8940 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 9000 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 9060 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 9120 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 9180 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 9240 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 9300 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 9360 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 9420 acatttcccc gaaaagtgcc acct 9444 //