LOCUS       PCD-F-SerpinB2         6636 bp    DNA     circular      7-JUN-2008
DEFINITION  LMBP 6357.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|484505937|
COMMENT     VNTDBDATE|484505937|
COMMENT     LSOWNER|
COMMENT     VNTNAME|PCD-F-SerpinB2|
COMMENT     VNTAUTHORNAME|Jens Staal|
COMMENT     VNTAUTHOREML|jens.staal@dmbr.ugent.be|
COMMENT     VNTAUTHORWWW|www.dmbr.ugent.be|
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FEATURES             Location/Qualifiers
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     polyA_signal    2225..2439
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      2502..2915
                     /vntifkey="33"
                     /label=f1
     promoter        2980..3305
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     3341..4135
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    4151..4390
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      4822..5495
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(5640..6501)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     primer          complement(2223..2239)
                     /vntifkey="27"
                     /label=1503
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     CDS             953..2200
                     /vntifkey="4"
                     /label=hSerpinB2/PAI2
BASE COUNT     1610 a      1675 c      1669 g      1682 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctatggagga 
      961 tctttgtgtg gcaaacacac tctttgccct caatttattc aagcatctgg caaaagcaag 
     1021 ccccacccag aacctcttcc tctccccatg gagcatctcg tccaccatgg ccatggtcta 
     1081 catgggctcc aggggcagca ccgaagacca gatggccaag gtgcttcagt ttaatgaagt 
     1141 gggagccaat gcagttaccc ccatgactcc agagaacttt accagctgtg ggttcatgca 
     1201 gcagatccag aagggtagtt atcctgatgc gattttgcag gcacaagctg cagataaaat 
     1261 ccattcatcc ttccgctctc tcagctctgc aatcaatgca tccacaggga attatttact 
     1321 ggaaagtgtc aataagctgt ttggtgagaa gtctgcgagc ttccgggaag aatatattcg 
     1381 actctgtcag aaatattact cctcagaacc ccaggcagta gacttcctag aatgtgcaga 
     1441 agaagctaga aaaaagatta attcctgggt caagactcaa accaaaggca aaatcccaaa 
     1501 cttgttacct gaaggttctg tagatgggga taccaggatg gtcctggtga atgctgtcta 
     1561 cttcaaagga aagtggaaaa ctccatttga gaagaaacta aatgggcttt atcctttccg 
     1621 tgtaaactcg gctcagcgca cacctgtaca gatgatgtac ttgcgtgaaa agctaaacat 
     1681 tggatacata gaagacctaa aggctcagat tctagaactc ccatatgctg gagatgttag 
     1741 catgttcttg ttgcttccag atgaaattgc cgatgtgtcc actggcttgg agctgctgga 
     1801 aagtgaaata acctatgaca aactcaacaa gtggaccagc aaagacaaaa tggctgaaga 
     1861 tgaagttgag gtatacatac cccagttcaa attagaagag cattatgaac tcagatccat 
     1921 tctgagaagc atgggcatgg aggacgcctt caacaaggga cgggccaatt tctcagggat 
     1981 gtcggagagg aatgacctgt ttctttctga agtgttccac caagccatgg tggatgtgaa 
     2041 tgaggagggc actgaagcag ccgctggcac aggaggtgtt atgacaggga gaactggaca 
     2101 tggaggccca cagtttgtgg cagatcatcc ttttcttttt cttattatgc ataagataac 
     2161 caactgcatt ttatttttcg gcagattttc ctcaccctaa gggcccgttt aaacccgctg 
     2221 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 
     2281 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 
     2341 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 
     2401 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 
     2461 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 
     2521 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
     2581 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
     2641 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga 
     2701 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
     2761 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
     2821 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc 
     2881 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 
     2941 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca 
     3001 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 
     3061 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 
     3121 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 
     3181 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 
     3241 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 
     3301 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 
     3361 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 
     3421 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 
     3481 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 
     3541 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 
     3601 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 
     3661 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 
     3721 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 
     3781 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 
     3841 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 
     3901 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 
     3961 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 
     4021 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 
     4081 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 
     4141 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 
     4201 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 
     4261 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 
     4321 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     4381 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 
     4441 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 
     4501 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 
     4561 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 
     4621 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 
     4681 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
     4741 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     4801 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     4861 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     4921 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     4981 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     5041 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 
     5101 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     5161 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     5221 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     5281 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 
     5341 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     5401 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     5461 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     5521 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     5581 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     5641 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     5701 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     5761 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     5821 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     5881 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     5941 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     6001 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     6061 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     6121 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     6181 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     6241 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     6301 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     6361 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     6421 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     6481 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     6541 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     6601 ccgcgcacat ttccccgaaa agtgccacct gacgtc 
//
