LOCUS       pCD-F-hCYLD-C           7215 bp    DNA     circular     15-SEP-2011
DEFINITION  LMBP 6358.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6358
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-SEP-2011
            
            
            
COMMENT     VNTDATE|601291371|
COMMENT     VNTNAME|pCD-F-hCYLD-C|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT      (CGexDoc "pCD-F-hCYLD-C" 0 7215 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 2785 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 951 0 0 "")) 
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COMMENT        #3=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 3920 4714 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3140..3934") 
COMMENT        #4=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6219 7079 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(5439..6299)") 
COMMENT        #5=(CFSignal (CObList) "pMB1 ori" 33 0 1 5276 5678 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4888 4962 0 
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COMMENT        #7=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4888 4962 0 
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COMMENT        #8=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 235 818 0 
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COMMENT        #9=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "hCYLD fragment" 4 0 0 953 2779 0 (CStringList) 
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COMMENT                          "DNA 'pCD-F-hCYLD-C'" 1) 
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COMMENT                          "Currently local object. Original author: Peter Marynen" 
COMMENT                          1) 
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COMMENT                          "Created: 09/15/11 09:22" 1) 
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COMMENT                          "length: 7215 bp" 1) 
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FEATURES             Location/Qualifiers
     promoter        3514..3796
                     /vntifkey="29"
                     /label=SV40
     CDS             3920..4714
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     CDS             complement(6219..7079)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(5276..5678)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      4888..4962
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4888..4962)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     923..946
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2811..3035
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      3081..3509
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      3753..3828
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             953..2779
                     /vntifkey="4"
                     /label=hCYLD\fragment
BASE COUNT     1803 a      1780 c      1771 g      1861 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac caatgaacag 
      961 atctgaatta ttttatacct taaatgggtc ttctgttgac tcacaaccac aatccaaatc 
     1021 aaaaaataca tggtacattg atgaagttgc agaagaccct gcaaaatctc ttacagagat 
     1081 atctacagac tttgaccgtt cttcaccacc actccagcct cctcctgtga actcactgac 
     1141 caccgagaac agattccact ctttaccatt cagtctcacc aagatgccca ataccaatgg 
     1201 aagtattggc cacagtccac tttctctgtc agcccagtct gtaatggaag agctaaacac 
     1261 tgcacccgtc caagagagtc cacccttggc catgcctcct gggaactcac atggtctaga 
     1321 agtgggctca ttggctgaag ttaaggagaa ccctcctttc tatggggtaa tccgttggat 
     1381 cggtcagcca ccaggactga atgaagtgct cgctggactg gaactggaag atgagtgtgc 
     1441 aggctgtacg gatggaacct tcagaggcac tcggtatttc acctgtgccc tgaagaaggc 
     1501 gctgtttgtg aaactgaaga gctgcaggcc tgactctagg tttgcatcat tgcagccggt 
     1561 ttccaatcag attgagcgct gtaactcttt agcatttgga ggctacttaa gtgaagtagt 
     1621 agaagaaaat actccaccaa aaatggaaaa agaaggcttg gagataatga ttgggaagaa 
     1681 gaaaggcatc cagggtcatt acaattcttg ttacttagac tcaaccttat tctgcttatt 
     1741 tgcttttagt tctgttctgg acactgtgtt acttagaccc aaagaaaaga acgatgtaga 
     1801 atattatagt gaaacccaag agctactgag gacagaaatt gttaatcctc tgagaatata 
     1861 tggatatgtg tgtgccacaa aaattatgaa actgaggaaa atacttgaaa aggtggaggc 
     1921 tgcatcagga tttacctctg aagaaaaaga tcctgaggaa ttcttgaata ttctgtttca 
     1981 tcatatttta agggtagaac ctttgctaaa aataagatca gcaggtcaaa aggtacaaga 
     2041 ttgttacttc tatcaaattt ttatggaaaa aaatgagaaa gttggcgttc ccacaattca 
     2101 gcagttgtta gaatggtctt ttatcaacag taacctgaaa tttgcagagg caccatcatg 
     2161 tctgattatt cagatgcctc gatttggaaa agactttaaa ctatttaaaa aaatttttcc 
     2221 ttctctggaa ttaaatataa cagatttact tgaagacact cccagacagt gccggatatg 
     2281 tggagggctt gcaatgtatg agtgtagaga atgctacgac gatccggaca tctcagctgg 
     2341 aaaaatcaag cagttttgta aaacctgcaa cactcaagtc caccttcatc cgaagaggct 
     2401 gaatcataaa tataacccag tgtcacttcc caaagactta cccgactggg actggagaca 
     2461 cggctgcatc ccttgccaga atatggagtt atttgctgtt ctctgcatag aaacaagcca 
     2521 ctatgttgct tttgtgaagt atgggaagga cgattctgcc tggctcttct ttgacagcat 
     2581 ggccgatcgg gatggtggtc agaatggctt caacattcct caagtcaccc catgcccaga 
     2641 agtaggagag tacttgaaga tgtctctgga agacctgcat tccttggact ccaggagaat 
     2701 ccaaggctgt gcacgaagac tgctttgtga tgcatatatg tgcatgtacc agagtccaac 
     2761 aatgagtttg tacaaataag ggcccgttta aacccgctga tcagcctcga ctgtgccttc 
     2821 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
     2881 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
     2941 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
     3001 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 
     3061 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     3121 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     3181 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat 
     3241 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     3301 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     3361 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     3421 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga 
     3481 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 
     3541 ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc 
     3601 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 
     3661 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 
     3721 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 
     3781 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 
     3841 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac 
     3901 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 
     3961 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 
     4021 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 
     4081 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 
     4141 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 
     4201 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 
     4261 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 
     4321 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 
     4381 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 
     4441 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 
     4501 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 
     4561 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 
     4621 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 
     4681 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 
     4741 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 
     4801 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 
     4861 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 
     4921 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 
     4981 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 
     5041 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 
     5101 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 
     5161 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     5221 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 
     5281 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     5341 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     5401 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     5461 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     5521 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     5581 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     5641 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     5701 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     5761 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     5821 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     5881 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     5941 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     6001 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     6061 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     6121 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     6181 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     6241 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     6301 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     6361 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     6421 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     6481 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     6541 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     6601 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     6661 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     6721 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     6781 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     6841 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     6901 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     6961 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     7021 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     7081 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     7141 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     7201 gtgccacctg acgtc 
//
