LOCUS       pCD-API-MALT-C464AC539A        8277 bp    DNA     circular     19-DEC-2008
DEFINITION  LMBP 6363.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|502108991|
COMMENT     VNTNAME|pCD-API-MALT-C464AC539A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 2112 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 6390 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 6372 0 8258 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 6 762 0 1998 0 2733 0 6080 
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COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 714 0 2354 0 6682 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 2114 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 2112 0 0 "")) 
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COMMENT        #24=(CFSignal (CObList) "SV40 pA" 25 0 0 2982 3221 1 (CStringList) 
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COMMENT        #25=(CFSignal (CObList) "Neomycin" 86 0 0 2172 2966 1 (CStringList) 
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COMMENT                                   "{\\i Nco}I (762)" "@N (@S)" 5 12.6282 
COMMENT                                   8.71165 0 -10 4.27096 0.924127 #350#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (1998)" "@N (@S)" 5 15.6634 
COMMENT                                   -3.48466 0 -10 4.72054 0.924127 #352#) 
COMMENT                             #354=(CScratch 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (2733)" "@N (@S)" 5 14.6789 
COMMENT                                   -13.9386 0 -10 4.79547 0.924127 #354#) 
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COMMENT                                   "{\\i Nco}I (6080)" "@N (@S)" 5 -15.6873 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (6401)" "@N (@S)" 5 -15.6211 
COMMENT                                   5.22699 0 -10 4.72054 0.924127 #358#) 
COMMENT                             #360=(CScratch 
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COMMENT        (CObList #366=(AnlzXLATItem 6404 8280 3 6404 1028) 
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FEATURES             Location/Qualifiers
     misc_feature    105..107
                     /vntifkey="21"
                     /label=C539A
     protein_bind    900..917
                     /vntifkey="31"
                     /label=TRAF6
     mutation        440..440
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation, G replaces A"
     protein_bind    450..581
                     /vntifkey="31"
                     /label=VDJ4
     modified_base   8255..8255
                     /vntifkey="23"
                     /label=stability\creator
                     /note="T replaces A as GCAGAAG recombines with the same heptanucleotide present in API2"
     mutation        8157..8159
                     /vntifkey="62"
                     /label=C464A
                     /note="Cys of caspase p20 like mutated to Ala"
     mutation        6557..6557
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation, T replaces C"
     mutation        6530..6530
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation, T replaces C"
     modified_base   6405..6405
                     /vntifkey="23"
                     /label=kozak
                     /note="A replaced by G to create Kozak consensus, AAC becomes GAC, D for N"
     protein_bind    7149..7367
                     /vntifkey="31"
                     /label=BIR3
     protein_bind    6891..7106
                     /vntifkey="31"
                     /label=BIR2
     protein_bind    6471..6689
                     /vntifkey="31"
                     /label=BIR1
     primer          6332..6351
                     /vntifkey="27"
                     /label=2506
                     /note="T7"
     misc_marker     complement(4471..5332)
                     /vntifkey="22"
                     /label=AmpR
     rep_origin      3653..4326
                     /vntifkey="33"
                     /label=Col\E1
     polyA_signal    2982..3221
                     /vntifkey="25"
                     /label=SV40\pA
     misc_recomb     2172..2966
                     /vntifkey="86"
                     /label=Neomycin
     promoter        1811..2136
                     /vntifkey="29"
                     /label=SV40\prom\ori
     rep_origin      1333..1746
                     /vntifkey="33"
                     /label=f1
     polyA_signal    1056..1270
                     /vntifkey="25"
                     /label=BGH\pA
     misc_feature    1017..1034
                     /vntifkey="21"
                     /label=6His
     misc_signal     972..1001
                     /vntifkey="87"
                     /label=Myc
     promoter        6332..6351
                     /vntifkey="30"
                     /label=T7
     misc_signal     5678..6332
                     /vntifkey="87"
                     /label=Pcmv
     protein_bind    7788..8189
                     /vntifkey="31"
                     /label=p20\like
     CDS             6405..1029
                     /vntifkey="4"
                     /label=IAP2-MALT
BASE COUNT     2140 a      1958 c      1996 g      2183 t 
ORIGIN
        1 ctggattggc aaatggaatc tttatgaaat ttttaaaaga cagattatta gaagataaga 
       61 aaatcactgt gttactggat gaagttgcag aagatatggg taaggctcac cttaccaaag 
      121 gcaaacaggc tctagagatt cgaagtagtt tatctgagaa gagagcactt actgatccaa 
      181 tacagggaac agaatattct gctgaatctc ttgtgcggaa tctacagtgg gccaaggctc 
      241 atgaacttcc agaaagtatg tgtcttaagt ttgactgtgg tgttcagatt caattaggat 
      301 ttgcagctga gttttccaat gtcatgatca tctatacaag tatagtttac aaaccaccgg 
      361 agataataat gtgtgatgcc tacgttactg attttccact tgatctagat attgatccaa 
      421 aagatgcaaa taaaggcacg cctgaagaaa ctggcagcta cttggtatca aaggatcttc 
      481 ccaagcattg cctctatacc agactcagtt cactgcaaaa attaaaggaa catctagtct 
      541 tcacagtatg tttatcatat cagtactcag gattggaaga tactgtagag gacaagcagg 
      601 aagtgaatgt tgggaaacct ctcattgcta aattagacat gcatcgaggt ttgggaagga 
      661 agacttgctt tcaaacttgt cttatgtcta atggtcctta ccagagttct gcagccacct 
      721 caggaggagc agggcattat cactcattgc aagacccatt ccatggtgtt taccattcac 
      781 atcctggtaa tccaagtaat gttacaccag cagatagctg tcattgcagc cggactccag 
      841 atgcatttat ttcaagtttc gctcaccatg cttcatgtca ttttagtaga agtaatgtgc 
      901 cagtagagac aactgatgaa ataccattta gtttctctga caggctcaga atttctgaaa 
      961 aagggccctt cgaacaaaaa ctcatctcag aagaggatct gaatatgcat accggtcatc 
     1021 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc 
     1081 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 
     1141 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 
     1201 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 
     1261 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 
     1321 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 
     1381 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 
     1441 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag 
     1501 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 
     1561 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 
     1621 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 
     1681 cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt 
     1741 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 
     1801 cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 
     1861 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 
     1921 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 
     1981 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 
     2041 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 
     2101 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag 
     2161 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 
     2221 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 
     2281 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 
     2341 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 
     2401 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 
     2461 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 
     2521 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 
     2581 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 
     2641 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 
     2701 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 
     2761 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 
     2821 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 
     2881 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 
     2941 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa 
     3001 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 
     3061 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 
     3121 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 
     3181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     3241 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 
     3301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
     3361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 
     3421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 
     3481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 
     3541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
     3601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 
     3661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
     3721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
     3781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
     3841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
     3901 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
     3961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
     4021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
     4081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     4141 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     4201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     4261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     4321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     4381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     4441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     4501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     4561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     4621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     4681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     4741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
     4801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     4861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     4921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     4981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     5041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
     5101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     5161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     5221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     5281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     5341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
     5401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     5461 cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct 
     5521 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 
     5581 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 
     5641 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc 
     5701 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 
     5761 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
     5821 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     5881 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 
     5941 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     6001 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
     6061 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 
     6121 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 
     6181 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 
     6241 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta 
     6301 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag 
     6361 ctggctagtt aagcttggta ccgagctcgg atcctgccac catggacata gtagaaaaca 
     6421 gcatattctt atcaaatttg atgaaaagcg ccaacacgtt tgaactgaaa tacgacttgt 
     6481 catgtgaact gtaccgaatg tctacgtatt ccacttttcc tgctggggtt cctgtctcag 
     6541 aaaggagtct tgctcgtgct ggtttctatt acactggtgt gaatgacaag gtcaaatgct 
     6601 tctgttgtgg cctgatgctg gataactgga aaagaggaga cagtcctact gaaaagcata 
     6661 aaaagttgta tcctagctgc agattcgttc agagtctaaa ttccgttaac aacttggaag 
     6721 ctacctctca gcctactttt ccttcttcag taacaaattc cacacactca ttacttccgg 
     6781 gtacagaaaa cagtggatat ttccgtggct cttattcaaa ctctccatca aatcctgtaa 
     6841 actccagagc aaatcaagat ttttctgcct tgatgagaag ttcctaccac tgtgcaatga 
     6901 ataacgaaaa tgccagatta cttacttttc agacatggcc attgactttt ctgtcgccaa 
     6961 cagatctggc aaaagcaggc ttttactaca taggacctgg agacagagtg gcttgctttg 
     7021 cctgtggtgg aaaattgagc aattgggaac cgaaggataa tgctatgtca gaacacctga 
     7081 gacattttcc caaatgccca tttatagaaa atcagcttca agacacttca agatacacag 
     7141 tttctaatct gagcatgcag acacatgcag cccgctttaa aacattcttt aactggccct 
     7201 ctagtgttct agttaatcct gagcagcttg caagtgcggg tttttattat gtgggtaaca 
     7261 gtgatgatgt caaatgcttt tgctgtgatg gtggactcag gtgttgggaa tctggagatg 
     7321 atccatgggt tcaacatgcc aagtggtttc caaggtgtga gtacttgata agaattaaag 
     7381 gacaggagtt catccgtcaa gttcaagcca gttaccctca tctacttgaa cagctgctat 
     7441 ccacatcaga cagcccagga gatgaaaatg cagagtcatc aattatccat tttgaacctg 
     7501 gagaagacca ttcagaagat gcaatcatga tgaatactcc tgtgattaat gctgccgtgg 
     7561 aaatgggctt tagtagaagc ctggtaaaac agacagttca gagaaaaatc ctagcaactg 
     7621 gagagaatta tagactagtc aatgatcttg tgttggactt actcaatgca gaagatgaaa 
     7681 taagggaaga ggagagagaa agagcaactg aggaaaaaga atcaaatgaa ttaaataatc 
     7741 ttggtcatcc tgataataaa gagcaaacaa ctgaccagcc tttggcgaag gacaaggttg 
     7801 cccttttgat aggaaatatg aattaccggg agcaccccaa gctcaaagct cctttggtgg 
     7861 atgtgtacga attgactaac ttactgagac agctggactt caaagtggtt tcactgttgg 
     7921 atcttactga atatgagatg cgtaatgctg tggatgagtt tttactcctt ttagacaagg 
     7981 gagtatatgg gttattatat tatgcaggac atggttatga aaattttggg aacagcttca 
     8041 tggtccccgt tgatgctcca aatccatata ggtctgaaaa ttgtctgtgt gtacaaaata 
     8101 tactgaaatt gatgcaagaa aaagaaactg gacttaatgt gttcttattg gatatggcgc 
     8161 gcaaaagaaa tgactacgat gataccattc caatcttgga tgcactaaaa gtcaccgcca 
     8221 atattgtgtt tggatatgcc acgtgtcaag gagctgaagc ttttgaaatc cagcatt 
//
