LOCUS       pCAGGS-GFP-hA20         7961 bp    DNA     circular      8-JUN-2011
DEFINITION  LMBP 6406.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6406
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 8-JUN-2011
            
            
COMMENT     VNTDATE|592392214|
COMMENT     VNTNAME|pCAGGS-GFP-hA20|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-GFP-hA20" 0 7961 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 311 311 1 7961 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT            "") 
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COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 833 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 5775 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 3035 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 3252 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 7957 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5331 6191 0 
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COMMENT        #9=(CFSignal (CObList) "pMB1 ori" 33 0 1 4389 4790 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4200 4274 0 
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COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4200 4274 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3424..3498") 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 4094 4169 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3318..3393") 
COMMENT        #13=(CFSignal (CObList) "lac promoter" 30 0 1 3870 3952 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3094..3176)") 
COMMENT        #14=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 3344 3435 0 (CStringList) (CStringList) 1 1 1 1 "2568..2659") 
COMMENT        #15=(CFSignal (CObList) "hA20" 4 0 0 840 3212 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "64..2436") 
COMMENT        #16=(CFSignal (CObList) "AG promoter" 29 0 0 6710 90 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 6327 6704 0 
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COMMENT        #18=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
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COMMENT        #19=(CFSignal (CObList) "3' UTR of hA20" 21 0 0 3213 3256 0 
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COMMENT        #20=(CFSignal (CObList) "GFP" 4 0 0 117 830 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
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COMMENT                          "Created: 06/08/11 09:23" 1) 
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COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7961 bp" 1) 
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FEATURES             Location/Qualifiers
     intron          7079..42
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(5331..6191)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(4389..4790)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(4200..4274)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      4200..4274
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      4094..4169
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(3870..3952)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      3344..3435
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     CDS             840..3212
                     /vntifkey="4"
                     /label=hA20
     promoter        6710..90
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        6327..6704
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    3436..3792
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     misc_feature    3213..3256
                     /vntifkey="21"
                     /label=3'\UTR\of\hA20
     CDS             117..830
                     /vntifkey="4"
                     /label=GFP
BASE COUNT     1898 a      2077 c      2094 g      1882 t        10 others
ORIGIN
        1 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg 
       61 gttattgtgc tgtctcatca ttttggcaaa gaattcctcg acatgannnn nnnnnnatgg 
      121 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg 
      181 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 
      241 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca 
      301 ctactttcac ttatggtgtt caatgctttt caagataccc agatcatatg aaacggcatg 
      361 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag 
      421 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 
      481 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg 
      541 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 
      601 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt 
      661 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 
      721 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 
      781 agtttgtaac agctgctggg attacacatg gcatggatga actatacaag gcggccgcaa 
      841 tggctgaaca agtccttcct caggctttgt atttgagcaa tatgcggaaa gctgtgaaga 
      901 tacgggagag aactccagaa gacattttta aacctactaa tgggatcatt catcatttta 
      961 aaaccatgca ccgatacaca ctggaaatgt tcagaacttg ccagttttgt cctcagtttc 
     1021 gggagatcat ccacaaagcc ctcatcgaca gaaacatcca ggccaccctg gaaagccaga 
     1081 agaaactcaa ctggtgtcga gaagtccgga agcttgtggc gctgaaaacg aacggtgacg 
     1141 gcaattgcct catgcatgcc acttctcagt acatgtgggg cgttcaggac acagacttgg 
     1201 tactgaggaa ggcgctgttc agcacgctca aggaaacaga cacacgcaac tttaaattcc 
     1261 gctggcaact ggagtctctc aaatctcagg aatttgttga aacggggctt tgctatgata 
     1321 ctcggaactg gaatgatgaa tgggacaatc ttatcaaaat ggcttccaca gacacaccca 
     1381 tggcccgaag tggacttcag tacaactcac tggaagaaat acacatattt gtcctttgca 
     1441 acatcctcag aaggccaatc attgtcattt cagacaaaat gctaagaagt ttggaatcag 
     1501 gttccaattt cgcccctttg aaagtgggtg gaatttactt gcctctccac tggcctgccc 
     1561 aggaatgcta cagatacccc attgttctcg gctatgacag ccatcatttt gtacccttgg 
     1621 tgaccctgaa ggacagtggg cctgaaatcc gagctgttcc acttgttaac agagaccggg 
     1681 gaagatttga agacttaaaa gttcactttt tgacagatcc tgaaaatgag atgaaggaga 
     1741 agctcttaaa agagtactta atggtgatag aaatccccgt ccaaggctgg gaccatggca 
     1801 caactcatct catcaatgcc gcaaagttgg atgaagctaa cttaccaaaa gaaatcaatc 
     1861 tggtagatga ttactttgaa cttgttcagc atgagtacaa gaaatggcag gaaaacagcg 
     1921 agcaggggag gagagagggg cacgcccaga atcccatgga accttccgtg ccccagcttt 
     1981 ctctcatgga tgtaaaatgt gaaacgccca actgcccctt cttcatgtct gtgaacaccc 
     2041 agcctttatg ccatgagtgc tcagagaggc ggcaaaagaa tcaaaacaaa ctcccaaagc 
     2101 tgaactccaa gccgggccct gaggggctcc ctggcatggc gctcggggcc tctcggggag 
     2161 aagcctatga gcccttggcg tggaaccctg aggagtccac tggggggcct cattcggccc 
     2221 caccgacagc acccagccct tttctgttca gtgagaccac tgccatgaag tgcaggagcc 
     2281 ccggctgccc cttcacactg aatgtgcagc acaacggatt ttgtgaacgt tgccacaacg 
     2341 cccggcaact tcacgccagc cacgccccag accacacaag gcacttggat cccgggaagt 
     2401 gccaagcctg cctccaggat gttaccagga catttaatgg gatctgcagt acttgcttca 
     2461 aaaggactac agcagaggcc tcctccagcc tcagcaccag cctccctcct tcctgtcacc 
     2521 agcgttccaa gtcagatccc tcgcggctcg tccggagccc ctccccgcat tcttgccaca 
     2581 gagctggaaa cgacgcccct gctggctgcc tgtctcaagc tgcacggact cctggggaca 
     2641 ggacggggac gagcaagtgc agaaaagccg gctgcgtgta ttttgggact ccagaaaaca 
     2701 agggcttttg cacactgtgt ttcatcgagt acagagaaaa caaacatttt gctgctgcct 
     2761 cagggaaagt cagtcccaca gcgtccaggt tccagaacac cattccgtgc ctggggaggg 
     2821 aatgcggcac ccttggaagc accatgtttg aaggatactg ccagaagtgt ttcattgaag 
     2881 ctcagaatca gagatttcat gaggccaaaa ggacagaaga gcaactgaga tcgagccagc 
     2941 gcagagatgt gcctcgaacc acacaaagca cctcaaggcc caagtgcgcc cgggcctcct 
     3001 gcaagaacat cctggcctgc cgcagcgagg agctctgcat ggagtgtcag catcccaacc 
     3061 agaggatggg ccctggggcc caccggggtg agcctgcccc cgaagacccc cccaagcagc 
     3121 gttgccgggc ccccgcctgt gatcattttg gcaatgccaa gtgcaacggc tactgcaacg 
     3181 aatgctttca gttcaagcag atgtatggct aaccggaaac aggtgggtca cctcctgcaa 
     3241 gaagtggggc ctcgaggtcg aggaattcac tcctcaggtg caggctgcct atcagaaggt 
     3301 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc 
     3361 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa 
     3421 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata 
     3481 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata 
     3541 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa 
     3601 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt 
     3661 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt 
     3721 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct 
     3781 cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc 
     3841 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     3901 gtaaagcctg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     3961 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc 
     4021 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 
     4081 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 
     4141 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta 
     4201 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     4261 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     4321 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 
     4381 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 
     4441 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat cagggataac 
     4501 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     4561 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     4621 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     4681 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     4741 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     4801 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     4861 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     4921 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     4981 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 
     5041 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     5101 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     5161 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     5221 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     5281 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     5341 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     5401 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     5461 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     5521 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     5581 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     5641 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     5701 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     5761 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     5821 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     5881 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     5941 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     6001 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     6061 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     6121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     6181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     6241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     6301 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc 
     6361 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 
     6421 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 
     6481 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 
     6541 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 
     6601 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 
     6661 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc 
     6721 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 
     6781 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg 
     6841 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa 
     6901 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta 
     6961 taaaaagcga agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg 
     7021 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt 
     7081 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct 
     7141 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg 
     7201 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc 
     7261 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag 
     7321 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg 
     7381 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc 
     7441 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc 
     7501 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg 
     7561 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg 
     7621 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc 
     7681 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc 
     7741 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg 
     7801 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc 
     7861 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca 
     7921 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag a 
//
