LOCUS       pCAGGS-E-DUBA             6567 bp    DNA     circular     25-SEP-2008
DEFINITION  LMBP 6413.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6413
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 25-SEP-2008
            
COMMENT     VNTDATE|494337298|
COMMENT     VNTNAME|pCAGGS-E-DUBA|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     intron          5592..6516
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3844..4704)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2902..3303)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2713..2787)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2713..2787
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      2607..2682
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(2383..2465)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1857..1948
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        5223..6564
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4840..5217
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1949..2305
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             63..1763
                     /vntifkey="4"
                     /label=DUBA
BASE COUNT     1374 a      1796 c      1909 g      1488 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcatgactat actccccaaa aagaagccgc cgcctcccga cgccgacccc gccaacgagc 
      121 cgccgccgcc cgggccgatg cccccggcgc cgcggcgcgg cggaggtgtg ggcgtgggcg 
      181 gcggcggcac gggcgtgggc ggcggcgatc gcgaccgtga ctccggcgtc gtgggggccc 
      241 gtccgcgagc ttcgccaccg cctcaaggcc cgctaccagg accgccgggc gctcttcatc 
      301 gctgggcgct ggccgtgccg cctggtgcag tggcgggtcc ccggccacaa caggcttctc 
      361 cacctccttg cgggggccca ggtggtcccg gcggcggtcc cggcgacgcg ctgggcgcag 
      421 cggcggcggg tgtgggtgcc gcgggcgtgg tggtgggtgt gggtggtgcc gtaggcgtgg 
      481 gcggctgctg ctccgggcct gggcacagca agcggcgacg tcaagctccc ggggttggcg 
      541 cggttggcgg gggcagtccc gagcgtgagg aggtcggcgc aggctacaac agtgaggacg 
      601 agtatgaggc ggctgcagca cgcatcgagg ctatggaccc tgccactgtc gagcagcagg 
      661 agcattggtt tgaaaaggcc ctacgagaca agaagggctt catcatcaag cagatgaagg 
      721 aggatggcgc ctgtctcttc cgggctgtag ctgaccaggt gtatggagac caggacatgc 
      781 atgaggttgt gcgaaagcat tgcatggact atctgatgaa gaatgccgac tacttctcca 
      841 actatgtcac agaggacttt accacctaca ttaacaggaa gcggaaaaac aattgccatg 
      901 gcaaccacat tgagatgcag gccatggcag agatgtacaa ccgtcctgtg gaggtgtacc 
      961 agtacagcac agaacccatc aacacattcc atgggataca tcaaaacgag gacgaaccca 
     1021 ttcgtgttag ctaccatcgg aatatccact ataattcagt ggtgaatcct aacaaggcca 
     1081 ccattggtgt ggggctgggc ctgccatcat tcaaaccagg gtttgcagag cagtctctga 
     1141 tgaagaatgc cataaaaaca tcggaggagt catggattga acagcagatg ctagaagaca 
     1201 agaaacgggc cacagactgg gaggccacaa atgaagccat cgaggagcag gtggctcggg 
     1261 aatcctacct gcagtggttg cgggatcagg agaaacaggc tcgccaggtc cgaggcccca 
     1321 gccagccccg gaaagccagc gccacatgca gttcggccac agcagcagcc tccagtggcc 
     1381 tggaggagtg gactagccgg tccccgcggc agcggagttc agcctcgtca cctgagcacc 
     1441 ctgagctgca tgctgaattg ggcatgaagc ccccttcccc aggcactgtt ttagctcttg 
     1501 ccaaacctcc ttcgccctgt gcgccaggta caagcagtca gttctcggca ggggccgacc 
     1561 gggcaacttc cccccttgtg tccctctacc ctgctttgga gtgccgggcc ctcattcagc 
     1621 agatgtcccc ctctgccttt ggtctgaatg actgggatga tgatgagatc ctagcttcgg 
     1681 tgctggcagt gtcccaacag gaatacctag acagtatgaa gaaaaacaaa gtgcacagag 
     1741 acccgccccc agacaagagt tgagtcgagg tcgaggaatt cactcctcag gtgcaggctg 
     1801 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc 
     1861 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg 
     1921 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac 
     1981 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga 
     2041 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc 
     2101 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt 
     2161 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat 
     2221 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag 
     2281 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg 
     2341 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     2401 ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     2461 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa 
     2521 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     2581 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     2641 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 
     2701 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     2761 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     2821 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga 
     2881 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     2941 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     3001 aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     3061 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     3121 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     3181 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     3241 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     3301 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     3361 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     3421 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     3481 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 
     3541 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     3601 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     3661 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     3721 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     3781 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     3841 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     3901 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     3961 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     4021 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     4081 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     4141 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     4201 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     4261 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     4321 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     4381 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     4441 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     4501 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     4561 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     4621 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     4681 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     4741 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     4801 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt 
     4861 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta 
     4921 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 
     4981 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 
     5041 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta 
     5101 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga 
     5161 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg 
     5221 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa 
     5281 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg 
     5341 ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggaaaggtg 
     5401 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc 
     5461 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc 
     5521 cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt 
     5581 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg 
     5641 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg 
     5701 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg 
     5761 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt 
     5821 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg 
     5881 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg 
     5941 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc 
     6001 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg 
     6061 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg 
     6121 gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc 
     6181 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa 
     6241 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg 
     6301 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc 
     6361 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg 
     6421 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct 
     6481 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt 
     6541 gtgctgtctc atcattttgg caaagaa 
//
