ID pCAGGS-E-hUNR standard; circular DNA; ; 7258 BP. DE LMBP 6421. CC Accession number: LMBP 6421 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 25-MAY-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 11..49 FT /vntifkey="22" FT /label=E-tag FT CDS complement(4530..5390) FT /vntifkey="4" FT /label=ampicillin FT intron 6278..7202 FT /vntifkey="15" FT /label=intron\AG\promoter FT promoter 5526..5903 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter 5909..7250 FT /vntifkey="29" FT /label=AG\promoter FT misc_feature 2635..2991 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of ß-globin gene" FT terminator complement(3399..3473) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT terminator 3399..3473 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT rep_origin complement(3588..3989) FT /vntifkey="33" FT /label=pMB1\ori FT terminator 2543..2634 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT promoter complement(3069..3151) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin 3293..3368 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT CDS 59..2455 FT /vntifkey="4" FT /label=hUNR FT /note="Human upstream of N-Ras" SQ Sequence 7258 BP; 1737 A; 1747 C; 1949 G; 1825 t; ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat 60 gagctttgat ccaaaccttc tccacaacaa tggacataat gggtacccta atggtacttc 120 agcagcactg cgtgaaactg gggttattga aaaactgtta acctcttacg gatttattca 180 gtgttcagaa cgtcaagcta gacttttctt ccactgttca cagtataatg gcaacctgca 240 agacttaaaa gtaggagatg atgttgaatt tgaagtatca tcggaccgac ggactgggaa 300 acccattgct gttaaactgg tgaagataaa acaagaaatc ctccctgaag aacgaatgaa 360 tggacaagtt gtgtgcgctg ttcctcacaa cttagagagt aaatctccag ctgccccggg 420 tcagagtcca acagggagtg tatgctacga acgtaatggg gaagtgtttt atctgactta 480 cacccctgaa gatgtcgaag ggaacgttca gctggaaact ggagataaaa taaactttgt 540 aattgataac aataaacata ctggtgctgt aagtgctcgc aacattatgc tgttgaaaaa 600 gaaacaagcc cgctgtcagg gagtagtttg tgccatgaag gaggcatttg gctttattga 660 aagaggtgat gttgtaaaag agatattctt tcactatagt gaatttaagg gtgacttaga 720 aaccttacag cctggcgatg atgtggaatt cacaatcaag gacagaaatg gtaaagaagt 780 tgcaacagat gtcagactat tgcctcaagg aacagtcatt tttgaagata tcagcattga 840 acattttgaa ggaactgtaa ccaaagttat cccaaaagta cccagtaaaa accagaatga 900 cccattgcca ggacgcatca aagttgactt tgtgatccct aaagaacttc cctttggaga 960 caaagatacg aaatccaagg tgaccctgct ggaaggtgac catgttaggt ttaatatttc 1020 aacagaccga cgtgacaaat tagagcgagc aaccaatata gaagttctgt caaatacatt 1080 tcagttcact aatgaagccc gagaaatggg tgtgattgct gccatgagag atggttttgg 1140 tttcatcaag tgtgtggatc gtgatgttcg tatgttcttc cacttcagtg aaattctgga 1200 tgggaaccag ctccatattg cagatgaagt agagtttact gtggttcctg atatgctctc 1260 tgctcaaaga aatcatgcta ttaggattaa aaaacttccc aagggcacgg tttcatttca 1320 ttcccattca gatcaccgtt ttctgggcac ggtagaaaaa gaagccactt tttccaatcc 1380 taaaaccact agcccaaata aaggcaaaga gaaggaggct gaggatggca ttattgctta 1440 tgatgactgt ggggtgaaac tgactattgc ttttcaagcc aaggatgtgg aaggatctac 1500 ttctcctcaa ataggagata aggttgaatt tagtattagt gacaaacaga ggcctggaca 1560 gcaggttgca acttgtgtgc gacttttagg tcgtaattct aactccaaga ggctcttggg 1620 ttatgtggca actctgaagg ataattttgg atttattgaa acagccaatc atgataagga 1680 aatctttttc cattacagtg agttctctgg tgatgttgat agcctggaac tgggggacat 1740 ggtcgagtat agcttgtcca aaggcaaagg caacaaagtc agtgcagaaa aagtgaacaa 1800 aacacactca gtgaatggca ttactgagga agctgatccc accatttact ctggcaaagt 1860 aattcgcccc ctgaggagtg ttgatccaac acagactgag taccaaggaa tgattgagat 1920 tgtggaggag ggcgatatga aaggtgaggt ctatccattt ggcatcgttg ggatggccaa 1980 caaaggggat tgcctgcaga aaggggagag cgtcaagttc caattgtgtg tcctgggcca 2040 aaatgcacaa actatggctt acaacatcac acccctgcgc agggccacag tggaatgtgt 2100 gaaagatcag tttggcttca ttaactatga agtaggagat agcaagaagc tctttttcca 2160 tgtgaaagaa gttcaggatg gcattgagct acaggcagga gatgaggtgg agttctcagt 2220 gattcttaat cagcgcactg gcaagtgcag cgcctgtaat gtttggcgag tctgtgaggg 2280 ccccaaggct gttgcagctc ctcgacctga tcggttggtc aatcgcttga agaatatcac 2340 tctggatgat gccagtgctc ctcgcctaat ggttcttcgt cagccaaggg gaccagataa 2400 ctcaatgggg tttggtgcag aaagaaagat ccgtcaagct ggtgtcattg attaactcga 2460 ggaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc 2520 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat 2580 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 2640 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa 2700 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat 2760 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc 2820 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt 2880 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct 2940 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct 3000 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 3060 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg 3120 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 3180 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3240 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3300 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 3360 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata 3420 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 3480 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca 3540 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 3600 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 3660 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca 3720 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 3780 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 3840 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 3900 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 3960 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 4020 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 4080 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 4140 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 4200 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 4260 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 4320 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 4380 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 4440 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 4500 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 4560 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 4620 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 4680 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 4740 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 4800 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 4860 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 4920 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 4980 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 5040 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 5100 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 5160 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 5220 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 5280 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 5340 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 5400 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 5460 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 5520 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 5580 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 5640 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 5700 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 5760 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 5820 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 5880 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc 5940 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 6000 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg 6060 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa 6120 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 6180 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg 6240 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 6300 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg 6360 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc 6420 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga 6480 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc 6540 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg 6600 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct 6660 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg 6720 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 6780 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg 6840 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga 6900 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc 6960 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg 7020 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct 7080 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 7140 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 7200 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgaa 7258 //