ID pROSA26-DV2 standard; circular DNA; ; 11751 BP. DE LMBP 6452. CC Accession number: LMBP 6452 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 1-AUG-2013 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_feature 1522..5753 FT /vntifkey="21" FT /label=3'\UTR\of\ROSA26 FT attenuator 1212..1336 FT /vntifkey="0" FT /label=attR4 FT CDS 866..1171 FT /vntifkey="4" FT /label=ccdB FT CDS 11616..524 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT attenuator complement(11383..11507) FT /vntifkey="0" FT /label=attR3 FT misc_feature 10251..11331 FT /vntifkey="21" FT /label=5'\UTR\of\ROSA26 FT CDS complement(8599..9459) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(9590..10045) FT /vntifkey="33" FT /label=f1\ori FT rep_origin complement(7656..8058) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(7401..7417) FT /vntifkey="30" FT /label=T3\promoter FT promoter complement(7481..7564) FT /vntifkey="30" FT /label=lac\promoter FT terminator 7107..7331 FT /vntifkey="43" FT /label=BGH\polyA FT promoter 5795..6296 FT /vntifkey="29" FT /label=mPGK1\promoter FT promoter 10213..10229 FT /vntifkey="30" FT /label=T7\promoter FT promoter 11515..11567 FT /vntifkey="30" FT /label=lacUV5\promoter SQ Sequence 11751 BP; 2955 A; 2596 C; 2898 G; 3299 t; 3 other; agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc 60 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg 120 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct 180 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt 240 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct 300 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact 360 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc 420 cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta 480 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatctaga ggatccggct 540 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat 600 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac 660 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc 720 ggttcggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa 780 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt 840 gccgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag 900 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat 960 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtcccccgtg aactttaccc 1020 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc 1080 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa 1140 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc 1200 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt 1260 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt 1320 ttctatacaa agttgatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 1380 gttatccgct cgtaatttaa atggcgcgcc ggctagaaga tgggcgggag tcttctgggc 1440 aggcttaaag gctaacctgg tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa 1500 aattggaggg acaagacttc ccacagattt tcggttttgt cgggaagttt tttaataggg 1560 gcaaataagg aaaatgggag gataggtagt catctggggt tttatgcagc aaaactacag 1620 gttattattg cttgtgatcc gcctcggagt attttccatc gaggtagatt aaagacatgc 1680 tcacccgagt tttatactct cctgcttgag atccttacta cagtatgaaa ttacagtgtc 1740 gcgagttaga ctatgtaagc agaattttaa tcatttttaa agagcccagt acttcatatc 1800 catttctccc gctccttctg cagccttatc aaaaggtatt ttagaacact cattttagcc 1860 ccattttcat ttattatact ggcttatcca acccctagac agagcattgg cattttccct 1920 ttcctgatct tagaagtctg atgactcatg aaaccagaca gattagttac atacaccaca 1980 aatcgaggct gtagctgggg cctcaacact gcagttcttt tataactcct tagtacactt 2040 tttgttgatc ctttgccttg atccttaatt ttcagtgtct atcacctctc ccgtcaggtg 2100 gtgttccaca tttgggccta ttctcagtcc agggagtttt acaacaatag atgtattgag 2160 aatccaacct aaagcttaac tttccactcc catgaatgcc tctctccttt ttctccattt 2220 ataaactgag ctattaacca ttaatggttt ccaggtggat gtctcctccc ccaatattac 2280 ctgatgtatc ttacatattg ccaggctgat attttaagac attaaaaggt atatttcatt 2340 attgagccac atggtattga ttactgctta ctaaaatttt gtcattgtac acatctgtaa 2400 aaggtggttc cttttggaat gcaaagttca ggtgtttgtt gtctttcctg acctaaggtc 2460 ttgtgagctt gtattttttc tatttaagca gtgctttctc ttggactggc ttgactcatg 2520 gcattctaca cgttattgct ggtctaaatg tgattttgcc aagcttcttc aggacctata 2580 attttgcttg acttgtagcc aaacacaagt aaaatgatta agcaacaaat gtatttgtga 2640 agcttggttt ttaggttgtt gtgttgtgtg tgcttgtgct ctataataat actatccagg 2700 ggctggagag gtggctcgga gttcaagagc acagactgct cttccagaag tcctgagttc 2760 aattcccagc aaccacatgg tggctcacaa ccatctgtaa tgggatctga tgccctcttc 2820 tggtgtgtct gaagaccaca agtgtattca cattaaataa ataaatcctc cttcttcttc 2880 tttttttttt ttttaaagag aatactgtct ccagtagaat ttactgaagt aatgaaatac 2940 tttgtgtttg ttccaatatg gtagccaata atcaaattac tctttaagca ctggaaatgt 3000 taccaaggaa ctaattttta tttgaagtgt aactgtggac agaggagcca taactgcaga 3060 cttgtgggat acagaagacc aatgcagact ttaatgtctt ttctcttaca ctaagcaata 3120 aagaaataaa aattgaactt ctagtatcct atttgttaaa ctgctagctt tacttaactt 3180 ttgtgcttca tctatacaaa gctgaaagct aagtctgcag ccattactaa acatgaaagc 3240 aagtaatgat aattttggat ttcaaaaatg tagggccaga gtttagccag ccagtggtgg 3300 tgcttgcctt tatgccttta atcccagcac tctggaggca gagacaggca gatctctgag 3360 tttgagccca gcctggtcta cacatcaagt tctatctagg atagccagga atacacacag 3420 aaaccctgtt ggggaggggg gctctgagat ttcataaaat tataattgaa gcattcccta 3480 atgagccact atggatgtgg ctaaatccgt ctacctttct gatgagattt gggtattatt 3540 ttttctgtct ctgctgttgg ttgggtcttt tgacactgtg ggctttcttt aaagcctcct 3600 tccctgccat gtggtctctt gtttgctact aacttcccat ggcttaaatg gcatggcttt 3660 ttgccttcta agggcagctg ctgagatttg cagcctgatt tccagggtgg ggttgggaaa 3720 tctttcaaac actaaaattg tcctttaatt ttttttttaa aaaatgggtt atataataaa 3780 cctcataaaa tagttatgag gagtgaggtg gactaatatt aaatgagtcc ctcccctata 3840 aaagagctat taaggctttt tgtcttatac ttaacttttt ttttaaatgt ggtatcttta 3900 gaaccaaggg tcttagagtt ttagtataca gaaactgttg catcgcttaa tcagattttc 3960 tagtttcaaa tccagagaat ccaaattctt cacagccaaa gtcaaattaa gaatttctga 4020 cttttaatgt taatttgctt actgtgaata taaaaatgat agcttttcct gaggcagggt 4080 ctcactatgt atctctgcct gatctgcaac aagatatgta gactaaagtt ctgcctgctt 4140 ttgtctcctg aatactaagg ttaaaatgta gtaatacttt tggaacttgc aggtcagatt 4200 cttttatagg ggacacacta agggagcttg ggtgatagtt ggtaaaatgt gtttcaagtg 4260 atgaaaactt gaattattat caccgcaacc tactttttaa aaaaaaaagc caggcctgtt 4320 agagcatgct taagggatcc ctaggacttg ctgagcacac aagagtagtt acttggcagg 4380 ctcctggtga gagcatattt caaaaaacaa ggcagacaac caagaaacta cagttaaggt 4440 tacctgtctt taaaccatct gcatatacac agggatatta aaatattcca aataatattt 4500 cattcaagtt ttcccccatc aaattgggac atggatttct ccggtgaata ggcagagttg 4560 gaaactaaac aaatgttggt tttgtgattt gtgaaattgt tttcaagtga tagttaaagc 4620 ccatgagata cagaacaaag ctgctatttc gaggtctctt ggtttatact cagaagcact 4680 tctttgggtt tccctgcact atcctgatca tgtgctaggc ctaccttagg ctgattgttg 4740 ttcaaataaa cttaagtttc ctgtcaggtg atgtcatatg atttcatata tcaaggcaaa 4800 acatgttata tatgttaaac atttgtactt aatgtgaaag ttaggtcttt gtgggttttg 4860 atttttaatt ttcaaaacct gagctaaata agtcattttt acatgtctta catttggtgg 4920 aattgtataa ttgtggtttg caggcaagac tctctgacct agtaacccta cctatagagc 4980 actttgctgg gtcacaagtc taggagtcaa gcatttcacc ttgaagttga gacgttttgt 5040 tagtgtatac tagtttatat gttggaggac atgtttatcc agaagatatt caggactatt 5100 tttgactggg ctaaggaatt gattctgatt agcactgtta gtgagcattg agtggccttt 5160 aggcttgaat tggagtcact tgtatatctc aaataatgct ggcctttttt aaaaagccct 5220 tgttctttat caccctgttt tctacataat ttttgttcaa agaaatactt gtttggatct 5280 ccttttgaca acaatagcat gttttcaagc catatttttt ttcctttttt tttttttttt 5340 tggtttttcg agacagggtt tctctgtata gccctggctg tcctggaact cactttgtag 5400 accaggctgg cctcgaactc agaaatccgc ctgcctctgc ctcctgagtg ccgggattaa 5460 aggcgtgcac caccacgcct ggctaagttg gatattttgt atataactat aaccaatact 5520 aactccactg ggtggatttt taattcagtc agtagtctta agtggtcttt attggccctt 5580 cattaaaatc tactgttcac tctaacagag gctgttggta ctagtggcac ttaagcaact 5640 tcctacggat atactagcag attaagggtc agggatagaa actagtctag cgttttgtat 5700 acctaccagc tttatactac cttgttctga tagaaatatt tcaggacatc tagcttatcg 5760 atccgtcgac ggtatcgata agcttgatat cgaattctac cgggtagggg aggcgctttt 5820 ccaaggcagt ctgagcatgc gcttagcagc cccgctggca cttggcgcta cacaagtggc 5880 ctytggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt 5940 ggccccttcg cgccaccttc twctcctccc ctagtcagga agttcccccc cgccccgcag 6000 ctcgcgtcgt saggacgtga caaatggaag tagcacgtct cactagtctc gtcagatgga 6060 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg 6120 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg 6180 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct 6240 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg 6300 caggtcctcg ccatggatcc tgatgatgtt gttattcttc taatcttttg tatggaaaac 6360 ttttcttcgt accacgggac taaacctggt tatgtagatt ccattcaaaa aggtatacaa 6420 aagccaaaat ctggtacaca aggaaattat gacgatgatt ggaaagggtt ttatagtacc 6480 gacaataaat acgacgctgc gggatactct gtagataatg aaaacccgct ctctggaaaa 6540 gctggaggcg tggtcaaagt gacgtatcca ggactgacga aggttctcgc actaaaagtg 6600 gataatgccg aaactattaa gaaagagtta ggtttaagtc tcactgaacc gttgatggag 6660 caagtcggaa cggaagagtt tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc 6720 agccttccct tcgctgaggg gagttctagc gttgaatata ttaataactg ggaacaggcg 6780 aaagcgttaa gcgtagaact tgagattaat tttgaaaccc gtggaaaacg tggccaagat 6840 gcgatgtatg agtatatggc tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt 6900 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag 6960 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt 7020 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcaga 7080 tcctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 7140 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 7200 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 7260 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 7320 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgacctcga gggggggccc 7380 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc 7440 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 7500 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 7560 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 7620 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 7680 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 7740 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 7800 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 7860 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 7920 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 7980 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 8040 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 8100 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 8160 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 8220 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 8280 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 8340 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 8400 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 8460 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 8520 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 8580 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 8640 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 8700 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 8760 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 8820 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 8880 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 8940 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 9000 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 9060 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 9120 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 9180 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 9240 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 9300 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 9360 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 9420 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 9480 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 9540 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc 9600 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa 9660 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt 9720 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 9780 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt 9840 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 9900 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 9960 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 10020 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa 10080 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca 10140 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc 10200 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg agctccccgc ggcaggccct 10260 ccgagcgtgg tggagccgtt ctgtgagaca gccgggtacg agtcgtgacg ctggaagggg 10320 caagcgggtg gtgggcagga atgcggtccg ccctgcagca accggagggg gagggagaag 10380 ggagcggaaa agtctccacc ggacgcggcc atggctcggg gggggggggg cagcggagga 10440 gcgcttccgg ccgacgtctc gtcgctgatt ggcttctttt cctcccgccg tgtgtgaaaa 10500 cacaaatggc gtgttttggt tggcgtaagg cgcctgtcag ttaacggcag ccggagtgcg 10560 cagccgccgg cagcctcgct ctgcccactg ggtggggcgg gaggtaggtg gggtgaggcg 10620 agctggacgt gcgggcgcgg tcggcctctg gcggggcggg ggaggggagg gagggtcagc 10680 gaaagtagct cgcgcgcgag cggccgccca ccctcccctt cctctggggg agtcgtttta 10740 cccgccgccg gccgggcctc gtcgtctgat tggctctcgg ggcccagaaa actggccctt 10800 gccattggct cgtgttcgtg caagttgagt ccatccgccg gccagcgggg gcggcgagga 10860 ggcgctccca ggttccggcc ctcccctcgg ccccgcgccg cagagtctgg ccgcgcgccc 10920 ctgcgcaacg tggcaggaag cgcgcgctgg gggcggggac gggcagtagg gctgagcggc 10980 tgcggggcgg gtgcaagcac gtttccgact tgagttgcct caagaggggc gtgctgagcc 11040 agacctccat cgcgcactcc ggggagtgga gggaaggagc gagggctcag ttgggctgtt 11100 ttggaggcag gaagcacttg ctctcccaaa gtcgctctga gttgttatca gtaagggagc 11160 tgcagtggag taggcgggga gaaggccgca cccttctccg gaggggggag gggagtgttg 11220 caataccttt ctgggagttc tctgctgcct cctggcttct gaggaccgcc ctgggcctgg 11280 gagaatccct tgccccctct tcccctcgtg atctgcaact ccagtctttc tagccttacg 11340 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tatcaactat gtataataaa 11400 gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa 11460 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag 11520 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg 11580 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata 11640 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg 11700 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta a 11751 //