ID pEntry\attL4pCAGG-loxP-Bgeo-3xpA-loxP-attR1 standard; circular DNA; ; 9905 BP. DE LMBP 6455. CC Accession number: LMBP 6455 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 13-DEC-2013 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT attenuator 2545..2640 FT /vntifkey="0" FT /label=attL4 FT attenuator complement(9899..117) FT /vntifkey="0" FT /label=attR1 FT CDS 346..1155 FT /vntifkey="4" FT /label=Tn903\neomycin\resistance FT promoter complement(196..212) FT /vntifkey="30" FT /label=T7\promoter FT terminator 2379..2422 FT /vntifkey="43" FT /label=T1\terminator FT terminator complement(2220..2247) FT /vntifkey="43" FT /label=T2\terminator FT misc_feature 9818..9851 FT /vntifkey="21" FT /label=loxP FT misc_feature 4877..4910 FT /vntifkey="21" FT /label=loxP FT rep_origin 1672..2074 FT /vntifkey="33" FT /label=pMB1\ori FT CDS 4965..8030 FT /vntifkey="4" FT /label=lacZ FT promoter 3129..3506 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT CDS 8055..8858 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT terminator 9155..9251 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(9155..9251) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 9392..9488 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(9392..9488) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 9629..9725 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(9629..9725) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter 3512..4853 FT /vntifkey="29" FT /label=AG\promoter FT intron 3881..4805 FT /vntifkey="15" FT /label=intron\AG\promoter FT mutation 8607..8609 FT /vntifkey="62" FT /label=E182D FT /note="WT: GAG" SQ Sequence 9905 BP; 2246 A; 2542 C; 2720 G; 2397 t; gtacaaaaaa gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat 60 tttgcataaa aaacagacta cataatactg taaaacacaa catatgcagt cactatgaac 120 caactactta gatggtatta gtgacctgta gaattaattc gagctctaga gctgcagggc 180 ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc 240 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga 300 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa 360 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa 420 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc 480 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat 540 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt 600 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc 660 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc 720 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt 780 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat 840 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca 900 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac 960 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag 1020 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt 1080 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc 1140 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg 1200 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc 1260 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 1320 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 1380 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 1440 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 1500 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 1560 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 1620 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 1680 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 1740 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 1800 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 1860 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 1920 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 1980 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 2040 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 2100 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 2160 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa 2220 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc 2280 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat 2340 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt 2400 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta 2460 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc 2520 gagttaacgc taccatggag ctccaaataa tgattttatt ttgactgata gtgacctgtt 2580 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtat agaaaagttg 2640 gctccgaatt cgccctttta aaggaaccaa ttcagtcgac tggatcactc aaccaagtca 2700 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 2760 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 2820 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 2880 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 2940 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 3000 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 3060 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 3120 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 3180 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 3240 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 3300 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 3360 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 3420 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 3480 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct 3540 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 3600 tgcagcgatg ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga 3660 ggggcggggc ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg 3720 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg 3780 gcgggcggga gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg 3840 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc 3900 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct 3960 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg 4020 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg 4080 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc 4140 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg 4200 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc 4260 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac 4320 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg 4380 cggggcgggg ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga 4440 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg 4500 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc 4560 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg 4620 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg 4680 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct 4740 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc 4800 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcc 4860 tcgatcgagg gacctaataa cttcgtatag catacattat acgaagttat attaagggtt 4920 ccgcaagctt cctagactag tcgacggtat cgataagcag cttgatgatc tgtgacatgg 4980 cggatcccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 5040 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 5100 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac 5160 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg 5220 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc 5280 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca 5340 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg 5400 ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc 5460 gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg 5520 tgatggtgct gcgttggagt gacggcagtt atctggaaga tcaggatatg tggcggatga 5580 gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc 5640 atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga 5700 tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc 5760 aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg 5820 ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc 5880 cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag 5940 aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg 6000 gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg 6060 tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg 6120 ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg 6180 gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc 6240 tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc 6300 gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg 6360 ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt 6420 atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg 6480 tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc 6540 tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc 6600 ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct 6660 tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt 6720 cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc 6780 tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt 6840 ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc 6900 atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg 6960 gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac 7020 cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga 7080 ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc 7140 tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg 7200 atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt 7260 cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc 7320 gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct 7380 gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca 7440 cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg 7500 ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga 7560 ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact 7620 gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact 7680 atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt 7740 ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt 7800 atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc 7860 aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata 7920 tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg 7980 aattccagct gagcgccggt cgctaccatt accagttggt ctggtgtcag gggatccccc 8040 gggctgcagc caatatggga tcggccattg aacaagatgg attgcacgca ggttctccgg 8100 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 8160 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 8220 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 8280 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 8340 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 8400 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 8460 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 8520 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 8580 ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc gatgcctgct 8640 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 8700 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 8760 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 8820 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg ggatcaattc tctaggcttg 8880 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag 8940 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgta aactacattc 9000 taattgtttg tgtattttag attcacagtc ccaacggctc atttcaggcc cctcagcctc 9060 acagtctgtt catgatcata atcagccata ccacatttgt agaggtttta cttgctttaa 9120 aaaacctccc acacctcccc ctgaactaac ataaaatgaa tgcaattgtt gttgttaact 9180 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 9240 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 9300 atgtctggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 9360 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 9420 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 9480 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 9540 tctggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc 9600 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta 9660 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 9720 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 9780 ggatccacta gttctagcta gtctaggtcg atgcaggata acttcgtata gcatacatta 9840 tacgaagtta tagatcttgg gtacccgctc gagatatcta gaaagggcga attcgaccca 9900 agttt 9905 //