LOCUS       pCD-F-DrMALT1-C444A        7818 bp    DNA     circular      1-FEB-2005
DEFINITION  LMBP 6462.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|365082567|
COMMENT     VNTNAME|pCD-F-DrMALT1-C444A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT             (CTerm 1 1 0 "Eco47III" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
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COMMENT                            5 1 "GGGCCC" "CCCGGG") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37743104 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #145=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #146=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2271 " 1) 
COMMENT                                      #147=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2777 " 1) 
COMMENT                                      #148=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 6896 " 1))))) "AspEI: 3 sites") 
COMMENT                            6 5 "GACNNNNNGTC" "CTGNNNNNCAG") 
COMMENT                      #149=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37743328 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #150=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #151=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4754 " 1) 
COMMENT                                      #152=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 7118 " 1))))) "AviII: 2 sites") 
COMMENT                            3 3 "TGCGCA" "ACGCGT") 
COMMENT                      #153=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37743552 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #154=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #155=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 956 " 1))))) "BamHI: 1 site") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #156=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37743624 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #157=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #158=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 13 " 1))))) "BglII: 1 site") 1 
COMMENT                            5 "AGATCT" "TCTAGA") 
COMMENT                      #159=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37743656 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BseAI: 0 sites") 1 5 "TCCGGA" 
COMMENT                            "AGGCCT") 
COMMENT                      #160=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37743688 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #161=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #162=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2292 " 1) 
COMMENT                                      #163=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5049 " 1))))) 
COMMENT                             "BssHII: 2 sites") 1 5 "GCGCGC" "CGCGCG") 
COMMENT                      #164=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37753536 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #165=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #10# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #166=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3119 " 1))))) "BstXI: 1 site") 
COMMENT                            8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                      #167=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37753760 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #168=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #11# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #169=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3918 " 1))))) "DraIII: 1 site") 
COMMENT                            6 3 "CACNNNGTG" "GTGNNNCAC") 
COMMENT                      #170=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37823464 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #171=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #12# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #172=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4558 " 1))))) "EclXI: 1 site") 
COMMENT                            1 5 "CGGCCG" "GCCGGC") 
COMMENT                      #173=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37827680 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #174=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #13# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #175=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2081 " 1))))) 
COMMENT                             "Eco47III: 1 site") 3 3 "AGCGCT" "TCGCGA") 
COMMENT                      #176=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37827784 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EcoNI: 0 sites") 5 6 "CCTNNNNNAGG" 
COMMENT                            "GGANNNNNTCC") 
COMMENT                      #177=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37741264 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #178=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #15# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #179=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3384 " 1))))) "EcoRI: 1 site") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #180=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37741336 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EcoRV: 0 sites") 3 3 "GATATC" 
COMMENT                            "CTATAG") 
COMMENT                      #181=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37751904 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #182=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #17# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #183=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4754 " 1) 
COMMENT                                      #184=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 7118 " 1))))) "FspI: 2 sites") 
COMMENT                            3 3 "TGCGCA" "ACGCGT") 
COMMENT                      #185=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37742432 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #186=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #18# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #187=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 912 " 1) 
COMMENT                                      #188=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2867 " 1) 
COMMENT                                      #189=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3143 " 1))))) 
COMMENT                             "HindIII: 3 sites") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #190=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37738976 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "HpaI: 0 sites") 3 3 "GTTAAC" 
COMMENT                            "CAATTG") 
COMMENT                      #191=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37780448 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #192=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #20# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #193=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 951 " 1) 
COMMENT                                      #194=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2181 " 1) 
COMMENT                                      #195=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3222 " 1))))) "KpnI: 3 sites") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
COMMENT                      #196=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37791104 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #197=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #21# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #198=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #21# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 229 " 1))))) "MluI: 1 site") 1 
COMMENT                            5 "ACGCGT" "TGCGCA") 
COMMENT                      #199=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37791136 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #200=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #22# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #201=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #22# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4734 " 1))))) "MluNI: 1 site") 
COMMENT                            3 3 "TGGCCA" "ACCGGT") 
COMMENT                      #202=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37782368 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #203=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #23# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #204=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3812 " 1) 
COMMENT                                      #205=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5154 " 1) 
COMMENT                                      #206=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 5437 " 1))))) "NaeI: 3 sites") 
COMMENT                            3 3 "GCCGGC" "CGGCCG") 
COMMENT                      #207=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37782400 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #208=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #24# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #209=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #24# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4652 " 1))))) "NarI: 1 site") 
COMMENT                            2 4 "GGCGCC" "CCGCGG") 
COMMENT                      #210=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37775400 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #211=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #25# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #212=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #25# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 611 " 1) 
COMMENT                                      #213=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #25# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 919 " 1) 
COMMENT                                      #214=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #25# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4349 " 1) 
COMMENT                                      #215=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #25# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 5084 " 1))))) "NcoI: 4 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #216=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37775432 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #217=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #26# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #218=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #26# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 485 " 1))))) "NdeI: 1 site") 2 
COMMENT                            4 "CATATG" "GTATAC") 
COMMENT                      #219=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37785112 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #220=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #27# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #221=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #27# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 896 " 1))))) "NheI: 1 site") 1 
COMMENT                            5 "GCTAGC" "CGATCG") 
COMMENT                      #222=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37785144 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" 
COMMENT                            "CGCCGGCG") 
COMMENT                      #223=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37779896 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #224=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #29# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #225=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 209 " 1))))) "NruI: 1 site") 3 
COMMENT                            3 "TCGCGA" "AGCGCT") 
COMMENT                      #226=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37779928 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #227=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #30# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #228=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #30# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1746 " 1) 
COMMENT                                      #229=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #30# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1775 " 1) 
COMMENT                                      #230=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #30# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2077 " 1) 
COMMENT                                      #231=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #30# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4192 " 1) 
COMMENT                                      #232=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #30# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 4264 " 1))))) "NsiI: 5 sites") 
COMMENT                            5 1 "ATGCAT" "TACGTA") 
COMMENT                      #233=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37785664 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PinAI: 0 sites") 1 5 "ACCGGT" 
COMMENT                            "TGGCCA") 
COMMENT                      #234=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37785696 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #235=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #32# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #236=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #32# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 905 " 1) 
COMMENT                                      #237=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #32# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3393 " 1))))) "PmeI: 2 sites") 
COMMENT                            4 4 "GTTTAAAC" "CAAATTTG") 
COMMENT                      #238=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37779344 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #239=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #33# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #240=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #33# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1874 " 1) 
COMMENT                                      #241=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #33# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2720 " 1) 
COMMENT                                      #242=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #33# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2941 " 1) 
COMMENT                                      #243=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #33# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4705 " 1))))) "PstI: 4 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
COMMENT                      #244=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37779376 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #245=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #34# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #246=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #34# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7266 " 1))))) "PvuI: 1 site") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
COMMENT                      #247=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37791968 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #248=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #35# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #249=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #35# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1058 " 1) 
COMMENT                                      #250=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #35# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1755 " 1) 
COMMENT                                      #251=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #35# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1976 " 1) 
COMMENT                                      #252=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #35# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 3661 " 1) 
COMMENT                                      #253=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #35# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 4758 " 1) 
COMMENT                                      #254=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #35# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 5827 " 1))))) "PvuII: 6 sites") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
COMMENT                      #255=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37792000 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #256=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #36# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #257=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 819 " 1) 
COMMENT                                      #258=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1366 " 1) 
COMMENT                                      #259=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1985 " 1) 
COMMENT                                      #260=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 2551 " 1) 
COMMENT                                      #261=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 3010 " 1))))) "SacI: 5 sites") 
COMMENT                            5 1 "GAGCTC" "CTCGAG") 
COMMENT                      #262=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37786288 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SacII: 0 sites") 4 2 "CCGCGG" 
COMMENT                            "GGCGCC") 
COMMENT                      #263=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37786320 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #264=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #38# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #265=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #38# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 33 " 1) 
COMMENT                                      #266=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #38# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5629 " 1) 
COMMENT                                      #267=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #38# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 7817 " 1))))) "SalI: 3 sites") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
COMMENT                      #268=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37792176 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #269=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #39# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #270=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2922 " 1) 
COMMENT                                      #271=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2998 (on complementary strand)" 
COMMENT                                            1) 
COMMENT                                      #272=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4996 (on complementary strand)" 
COMMENT                                            1) 
COMMENT                                      #273=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 5206 (on complementary strand)" 
COMMENT                                            1) 
COMMENT                                      #274=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #39# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 5887 " 1))))) "SapI: 5 sites") 
COMMENT                            8 11 "GCTCTTCNNNNN" "CGAGAAGNNNNN") 
COMMENT                      #275=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37792208 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #276=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #40# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #277=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #40# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7376 " 1))))) "ScaI: 1 site") 
COMMENT                            3 3 "AGTACT" "TCATGA") 
COMMENT                      #278=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 37786632 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #279=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #41# 0) 1 2 2 0 180) 
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FEATURES             Location/Qualifiers
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     polyA_signal    3407..3621
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      3684..4097
                     /vntifkey="33"
                     /label=f1
     promoter        4162..4487
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     4523..5317
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    5333..5572
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      6004..6677
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(6822..7683)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     primer          complement(3405..3421)
                     /vntifkey="27"
                     /label=1503
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     CDS             962..3379
                     /vntifkey="4"
                     /label=Drmlt-C444A
     mutation        2291..2293
                     /vntifkey="62"
                     /label=C444A
BASE COUNT     1915 a      1948 c      1962 g      1993 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc 
      961 tatggaggac aagaatttgg ggttagaaaa cataagtgag gcagcactta gtagacttgg 
     1021 ttacatgcta gacaatgcag agtgtggctg gaaacagctg gcaaaggcag tggctgagca 
     1081 gccacagttc tgctatagtg acagagagat gaccgactgc tcactcaaag tgctaagtcc 
     1141 acatggcagt cctggcagat acctgcttgc cctccttgcg gatagaggct gtacgttggc 
     1201 tttcctgctt cagtgcctga agaaaatcga gcaccgagag gctgttggat ttctcactat 
     1261 gaatgtcatg gagcagattg acatcataca gcagccacag agtcagcgga tagcagaggg 
     1321 gagtcctgtg gttctgagct gcaaagcagt cggtcctgta gagctccaat accagtggtt 
     1381 caagggcaaa gatgagatgc cagaaggtaa cagcccagat ttggtgttta atcgtgcaag 
     1441 tccagcacaa caggggtact acatctgtcg tgtaagttct ggagacaagt acatcttctc 
     1501 caactgggct catgtatatc tgctgaggtc tggaggtgcg agtgcagatt ccagcagcag 
     1561 ctacttccct tcaacagcta gtgggttgaa tataacacaa caacccaggc ctcaagcgtt 
     1621 aatggaggga gacacgctct gtcttgaatg ttctgcccag gccaacccgc ctccacagtt 
     1681 ccagtggtat tttaacaaag aacttatgcc aaagtgcata agaaacttct taaagattcc 
     1741 atgcataaca acagctgaca gaggtattta tgcatgcaga gtttacaacc tttatcacga 
     1801 aatgtggagt gatcaggtcc aagtaaacat tggtcctggt tcctgttcgg aaagctcatg 
     1861 ggaaaaaact gcagtagata atcctgaggc gaatctaaag caaatgggtg attgttgcgc 
     1921 cactgataag gtagcactac ttattgggaa catgaattac ctgcatcatc ggcagctgag 
     1981 agctcctatg gcggatgttc acgagctgac taaccttcta cgccagctag attttaaagt 
     2041 ggtttctctg ctggatctca actggcagga gatgcatagc gctgttactg agttcctgct 
     2101 gctgttggat cgaggagttt atggcctgtt gtattttgct ggtcatggtt atgagaacta 
     2161 tgggaacagt tttatggtac ccattgatgc tccagcctcc tacaccttca agcattgtct 
     2221 gtgggtgcag gaagtgttac agcggatgca agaacgccag acaggactta acgtctttct 
     2281 gctggacatg gcgcgcaaac gaaacccaaa cgatgagggc atcccacaac cggacccctt 
     2341 gagagtgaca gcaaacatag tgtttggcta tgctacgtgt gttgatgctg aagcatttga 
     2401 agtaaataaa gacgatctct ctaatggaat cttcatgagc ttcctgaaga aaagactaat 
     2461 ggagccagag aaggtcacgg tcatacttga caaagtagca gaagacatgg gaaggtgtga 
     2521 aatcactcgt ggtcggcagg ctctagagct ccgtagtaat ctatcagagc ggcgtgcttt 
     2581 gacagacaaa attcaggctc cagcatgcca aatcgcagca tcaacacgga atttacagtg 
     2641 ggccattgca catgtcctcc cagagagccg ctgtcttcag tttgaatgtg gtgtcacggt 
     2701 gcagctcggt tttgctgcag agttttccaa tatcatgata atctacactc agatattgga 
     2761 gactccaaca gacattgtgt cctgctctgc tcagctttct gattttactg agggccctga 
     2821 agttgacctg aagcgcagca atcaggagag tctccacgag gctgaaagct tcctgctgac 
     2881 catagatgat ctactgcctc tgaaacaacc tcagctcttc actcgtatta gtgccctgca 
     2941 gagactcaag aaagaactct cctttactgt gtgtctacac tacaagtatg gtaatctgga 
     3001 tgaagagctc ctggaagaga aaaggatcac tgtgggaaaa cctctagtgt ccaaactcaa 
     3061 tctccacgag ccacggctct ttcacagttc ctcctctgat caacaaatgt ccagtttttt 
     3121 ggactcttca tccttcagcg aaagcttcag agctaacctg cctgacctaa aacaaaattt 
     3181 cattggttct gcatctacat ccttgcccaa ctatcgggta ccaatggaat catttgcatg 
     3241 ctttggtttg aagaacgaac cggaggaaac attatgtcca gagtttcttg agtctgaaga 
     3301 acctcccgcc attaagagtt tacccagtgc ttcttctgag gaactgccat ttaaatttag 
     3361 caactttcaa aattccattt aggaattcgt ttaaacccgc tgatcagcct cgactgtgcc 
     3421 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 
     3481 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 
     3541 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 
     3601 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 
     3661 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     3721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     3781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     3841 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     3901 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     3961 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     4021 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa 
     4081 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 
     4141 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta 
     4201 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     4261 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     4321 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     4381 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     4441 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 
     4501 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     4561 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     4621 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     4681 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 
     4741 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     4801 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     4861 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     4921 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     4981 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     5041 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     5101 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     5161 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     5221 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     5281 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 
     5341 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 
     5401 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 
     5461 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 
     5521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     5581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 
     5641 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 
     5701 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 
     5761 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     5821 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     5881 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     5941 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 
     6001 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     6061 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     6121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     6181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     6241 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     6301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     6361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     6421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     6481 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 
     6541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     6601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     6661 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     6721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     6781 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     6841 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     6901 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     6961 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     7021 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     7081 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     7141 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     7201 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     7261 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     7321 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     7381 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     7441 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     7501 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     7561 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     7621 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     7681 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     7741 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     7801 aaagtgccac ctgacgtc 
//
