LOCUS       pCAGGS-E-CYLD           7736 bp    DNA     circular      3-DEC-2013
DEFINITION  LMBP 6613.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6613
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 03-DEC-2013
COMMENT     VNTDATE|680457606|
COMMENT     VNTNAME|pCAGGS-E-CYLD|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-CYLD" 0 7736 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
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COMMENT        (CWordArray) (CStringList "NotI" "PvuI" "SalI" "XhoI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 1 59 2929 1 7736 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 52 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 2738 0 5457 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 6007 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2934 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 4 481 0 1302 0 2757 0 
COMMENT            5686 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #6=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 0 
COMMENT            3118 3474 0 (CStringList) (CStringList) 1 1 1 1 "3115..3471") 
COMMENT        #7=(CFSignal (CObList) "lac promoter" 30 0 1 3552 3634 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3549..3631)") 
COMMENT        #8=(CFSignal (CObList) "SV40 ori" 33 0 0 3776 3851 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3773..3848") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3882 3956 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3879..3953") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3882 3956 0 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 4071 4472 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5013 5873 0 
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COMMENT        #13=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 6009 6386 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6006..6383") 
COMMENT        #14=(CFSignal (CObList) "AG promoter" 29 0 0 6392 7733 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "intron AG promoter" 15 0 0 6761 7685 0 
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COMMENT        #17=(CFSignal (CObList) "hCYLD" 4 0 0 59 2929 0 (CStringList) 
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COMMENT                          "Created: 12/03/13 04:00" 1) 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "E. coli lac operon promoter" 1) 
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COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     terminator      3026..3117
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    3118..3474
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(3552..3634)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      3776..3851
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      3882..3956
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(3882..3956)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(4071..4472)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(5013..5873)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        6009..6386
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        6392..7733
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          6761..7685
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     CDS             11..49
                     /vntifkey="4"
                     /label=E-tag
     CDS             59..2929
                     /vntifkey="4"
                     /label=hCYLD
BASE COUNT     1891 a      1856 c      2023 g      1966 t 
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgcaat 
       61 gagttcaggc ttatggagcc aagaaaaagt cacttcaccc tactgggaag agcggatttt 
      121 ttacttgctt cttcaagaat gcagcgttac agacaaacaa acacaaaagc tccttaaagt 
      181 accgaaggga agtataggac agtatattca agatcgttct gtggggcatt caaggattcc 
      241 ttctgcaaaa ggcaagaaaa atcagattgg attaaaaatt ctagagcaac ctcatgcagt 
      301 tctctttgtt gatgaaaagg atgttgtaga gataaatgaa aagttcacag agttactttt 
      361 ggcaattacc aattgtgagg agaggttcag cctgtttaaa aacagaaaca gactaagtaa 
      421 aggcctccaa atagacgtgg gctgtcctgt gaaagtacag ctgagatctg gggaagaaaa 
      481 atttcctgga gttgtacgct tcagaggacc cctgttagca gagaggacag tctccggaat 
      541 attctttgga gttgaattgc tggaagaagg tcgtggtcaa ggtttcactg acggggtgta 
      601 ccaagggaaa cagctttttc agtgtgatga agattgtggc gtgtttgttg cattggacaa 
      661 gctagaactc atagaagatg atgacactgc attggaaagt gattacgcag gtcctgggga 
      721 cacaatgcag gtcgaacttc ctcctttgga aataaactcc agagtttctt tgaaggttgg 
      781 agaaacaata gaatctggaa cagttatatt ctgtgatgtt ttgccaggaa aagaaagctt 
      841 aggatatttt gttggtgtgg acatggataa ccctattggc aactgggatg gaagatttga 
      901 tggagtgcag ctttgtagtt ttgcgtgtgt tgaaagtaca attctattgc acatcaatga 
      961 tatcatccca gctttatcag agagtgtgac gcaggaaagg aggcctccca aacttgcctt 
     1021 tatgtcaaga ggtgttgggg acaaaggttc atccagtcat aataaaccaa aggctacagg 
     1081 atctacctca gaccctggaa atagaaacag atctgaatta ttttatacct taaatgggtc 
     1141 ttctgttgac tcacaaccac aatccaaatc aaaaaataca tggtacattg atgaagttgc 
     1201 agaagaccct gcaaaatctc ttacagagat atctacagac tttgaccgtt cttcaccacc 
     1261 actccagcct cctcctgtga actcactgac caccgagaac agattccact ctttaccatt 
     1321 cagtctcacc aagatgccca ataccaatgg aagtattggc cacagtccac tttctctgtc 
     1381 agcccagtct gtaatggaag agctaaacac tgcacccgtc caagagagtc cacccttggc 
     1441 catgcctcct gggaactcac atggtctaga agtgggctca ttggctgaag ttaaggagaa 
     1501 ccctcctttc tatggggtaa tccgttggat cggtcagcca ccaggactga atgaagtgct 
     1561 cgctggactg gaactggaag atgagtgtgc aggctgtacg gatggaacct tcagaggcac 
     1621 tcggtatttc acctgtgccc tgaagaaggc gctgtttgtg aaactgaaga gctgcaggcc 
     1681 tgactctagg tttgcatcat tgcagccggt ttccaatcag attgagcgct gtaactcttt 
     1741 agcatttgga ggctacttaa gtgaagtagt agaagaaaat actccaccaa aaatggaaaa 
     1801 agaaggcttg gagataatga ttgggaagaa gaaaggcatc cagggtcatt acaattcttg 
     1861 ttacttagac tcaaccttat tctgcttatt tgcttttagt tctgttctgg acactgtgtt 
     1921 acttagaccc aaagaaaaga acgatgtaga atattatagt gaaacccaag agctactgag 
     1981 gacagaaatt gttaatcctc tgagaatata tggatatgtg tgtgccacaa aaattatgaa 
     2041 actgaggaaa atacttgaaa aggtggaggc tgcatcagga tttacctctg aagaaaaaga 
     2101 tcctgaggaa ttcttgaata ttctgtttca tcatatttta agggtagaac ctttgctaaa 
     2161 aataagatca gcaggtcaaa aggtacaaga ttgttacttc tatcaaattt ttatggaaaa 
     2221 aaatgagaaa gttggcgttc ccacaattca gcagttgtta gaatggtctt ttatcaacag 
     2281 taacctgaaa tttgcagagg caccatcatg tctgattatt cagatgcctc gatttggaaa 
     2341 agactttaaa ctatttaaaa aaatttttcc ttctctggaa ttaaatataa cagatttact 
     2401 tgaagacact cccagacagt gccggatatg tggagggctt gcaatgtatg agtgtagaga 
     2461 atgctacgac gatccggaca tctcagctgg aaaaatcaag cagttttgta aaacctgcaa 
     2521 cactcaagtc caccttcatc cgaagaggct gaatcataaa tataacccag tgtcacttcc 
     2581 caaagactta cccgactggg actggagaca cggctgcatc ccttgccaga atatggagtt 
     2641 atttgctgtt ctctgcatag aaacaagcca ctatgttgct tttgtgaagt atgggaagga 
     2701 cgattctgcc tggctcttct ttgacagcat ggccgatcgg gatggtggtc agaatggctt 
     2761 caacattcct caagtcaccc catgcccaga agtaggagag tacttgaaga tgtctctgga 
     2821 agacctgcat tccttggact ccaggagaat ccaaggctgt gcacgaagac tgctttgtga 
     2881 tgcatatatg tgcatgtacc agagtccaac aatgagtttg tacaaataag acctcgaggt 
     2941 cgaggaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa 
     3001 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat 
     3061 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat 
     3121 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt 
     3181 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc 
     3241 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca 
     3301 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt 
     3361 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt 
     3421 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga 
     3481 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     3541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tgggtgccta 
     3601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     3661 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact 
     3721 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 
     3781 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 
     3841 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt 
     3901 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 
     3961 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct 
     4021 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
     4081 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     4141 ctcaaaggcg gtaatacggt tatccacaga atcagggata acgcaggaaa gaacatgtga 
     4201 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
     4261 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
     4321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
     4381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
     4441 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
     4501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
     4561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
     4621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     4681 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     4741 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     4801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     4861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     4921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     4981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     5041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     5101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     5161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     5221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     5281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
     5341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     5401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     5461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     5521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     5581 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
     5641 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     5701 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     5761 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     5821 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     5881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
     5941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     6001 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 
     6061 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 
     6121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 
     6181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 
     6241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 
     6301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 
     6361 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct 
     6421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 
     6481 tgcagcgatg ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga 
     6541 ggggcggggc ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg 
     6601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg 
     6661 gcgggcggga gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg 
     6721 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc 
     6781 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct 
     6841 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg 
     6901 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg 
     6961 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc 
     7021 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg 
     7081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc 
     7141 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac 
     7201 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg 
     7261 cggggcgggg ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga 
     7321 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg 
     7381 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc 
     7441 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg 
     7501 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg 
     7561 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct 
     7621 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc 
     7681 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa 
//
