LOCUS       pCAGGS-E-hCYLD-C        6766 bp    DNA     circular     15-SEP-2011
DEFINITION  LMBP 6615.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6615
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-SEP-2011
            
            
            
            
COMMENT     VNTDATE|601302290|
COMMENT     VNTNAME|pCAGGS-E-hCYLD-C|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hCYLD-C" 0 6766 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "NotI" "PvuI" "SacI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6766 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 2706 0 3043 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 4 0 1142 0 1976 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 1771 0 4487 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1964 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "intron AG promoter" 15 0 0 5791 6715 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6303..7227") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4043 4903 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4555..5415)") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 3101 3502 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3613..4014)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2912 2986 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3424..3498)") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2912 2986 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3424..3498") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 2806 2881 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3318..3393") 
COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 2582 2664 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3094..3176)") 
COMMENT        #13=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 2056 2147 0 (CStringList) (CStringList) 1 1 1 1 "2568..2659") 
COMMENT        #14=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #15=(CFSignal (CObList) "AG promoter" 29 0 0 5422 6763 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5934..7275") 
COMMENT        #16=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 5039 5416 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #17=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 2148 2504 0 (CStringList) (CStringList) 1 1 1 1 "2660..3016") 
COMMENT        #18=(CFSignal (CObList) "hCYLD fragment" 4 0 0 64 1962 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #19=(CFSignal (CObList) "LMBP 6615" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "Last modified: 15-SEP-2011" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-hCYLD-C'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 09/15/11 12:24" 1) 
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COMMENT                          "Last Modified: 09/15/11 12:36" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6766 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 25-SEP-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                 "" 1))) "LMBP 6615") 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 64    End: 1962" 1))) 
COMMENT                                 "hCYLD fragment") 
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COMMENT                                        "   complement(4555..5415)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
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COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                        "   2660..3016" 1))) 
COMMENT                                 "3' flanking region of rabbit \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5551..5928" 1))) "hCMV-IE enhancer") 
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COMMENT                                        "Start: 5422  End: 6763" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #75=(CLinePar 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                        "   5934..7275" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #81=(CLinePar 
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COMMENT                                        "   complement(3094..3176)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "   complement(3613..4014)" 1))) 
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COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     intron          5791..6715
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(4043..4903)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(3101..3502)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2912..2986)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2912..2986
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      2806..2881
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(2582..2664)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      2056..2147
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        5422..6763
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        5039..5416
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    2148..2504
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..1962
                     /vntifkey="4"
                     /label=hCYLD\fragment
BASE COUNT     1593 a      1694 c      1775 g      1704 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcaggtgttg gggacaaagg ttcatccagt cataataaac caaaggctac aggatctacc 
      121 tcagaccctg gaaatagaaa cagatctgaa ttattttata ccttaaatgg gtcttctgtt 
      181 gactcacaac cacaatccaa atcaaaaaat acatggtaca ttgatgaagt tgcagaagac 
      241 cctgcaaaat ctcttacaga gatatctaca gactttgacc gttcttcacc accactccag 
      301 cctcctcctg tgaactcact gaccaccgag aacagattcc actctttacc attcagtctc 
      361 accaagatgc ccaataccaa tggaagtatt ggccacagtc cactttctct gtcagcccag 
      421 tctgtaatgg aagagctaaa cactgcaccc gtccaagaga gtccaccctt ggccatgcct 
      481 cctgggaact cacatggtct agaagtgggc tcattggctg aagttaagga gaaccctcct 
      541 ttctatgggg taatccgttg gatcggtcag ccaccaggac tgaatgaagt gctcgctgga 
      601 ctggaactgg aagatgagtg tgcaggctgt acggatggaa ccttcagagg cactcggtat 
      661 ttcacctgtg ccctgaagaa ggcgctgttt gtgaaactga agagctgcag gcctgactct 
      721 aggtttgcat cattgcagcc ggtttccaat cagattgagc gctgtaactc tttagcattt 
      781 ggaggctact taagtgaagt agtagaagaa aatactccac caaaaatgga aaaagaaggc 
      841 ttggagataa tgattgggaa gaagaaaggc atccagggtc attacaattc ttgttactta 
      901 gactcaacct tattctgctt atttgctttt agttctgttc tggacactgt gttacttaga 
      961 cccaaagaaa agaacgatgt agaatattat agtgaaaccc aagagctact gaggacagaa 
     1021 attgttaatc ctctgagaat atatggatat gtgtgtgcca caaaaattat gaaactgagg 
     1081 aaaatacttg aaaaggtgga ggctgcatca ggatttacct ctgaagaaaa agatcctgag 
     1141 gaattcttga atattctgtt tcatcatatt ttaagggtag aacctttgct aaaaataaga 
     1201 tcagcaggtc aaaaggtaca agattgttac ttctatcaaa tttttatgga aaaaaatgag 
     1261 aaagttggcg ttcccacaat tcagcagttg ttagaatggt cttttatcaa cagtaacctg 
     1321 aaatttgcag aggcaccatc atgtctgatt attcagatgc ctcgatttgg aaaagacttt 
     1381 aaactattta aaaaaatttt tccttctctg gaattaaata taacagattt acttgaagac 
     1441 actcccagac agtgccggat atgtggaggg cttgcaatgt atgagtgtag agaatgctac 
     1501 gacgatccgg acatctcagc tggaaaaatc aagcagtttt gtaaaacctg caacactcaa 
     1561 gtccaccttc atccgaagag gctgaatcat aaatataacc cagtgtcact tcccaaagac 
     1621 ttacccgact gggactggag acacggctgc atcccttgcc agaatatgga gttatttgct 
     1681 gttctctgca tagaaacaag ccactatgtt gcttttgtga agtatgggaa ggacgattct 
     1741 gcctggctct tctttgacag catggccgat cgggatggtg gtcagaatgg cttcaacatt 
     1801 cctcaagtca ccccatgccc agaagtagga gagtacttga agatgtctct ggaagacctg 
     1861 cattccttgg actccaggag aatccaaggc tgtgcacgaa gactgctttg tgatgcatat 
     1921 atgtgcatgt accagagtcc aacaatgagt ttgtacaaat aactcgaggt cgaggaattc 
     1981 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct 
     2041 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc 
     2101 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg 
     2161 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag 
     2221 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa 
     2281 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc 
     2341 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt 
     2401 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc 
     2461 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc 
     2521 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     2581 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc 
     2641 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     2701 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 
     2761 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     2821 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 
     2881 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta 
     2941 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     3001 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga 
     3061 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     3121 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     3181 gtaatacggt tatccacaga atcagggata acgcaggaaa gaacatgtga gcaaaaggcc 
     3241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     3301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     3361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     3421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     3481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     3541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     3601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     3661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     3721 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     3781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     3841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     3901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     3961 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     4021 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     4081 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     4141 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     4201 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     4261 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     4321 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     4381 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     4441 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     4501 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     4561 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     4621 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     4681 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     4741 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     4801 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     4861 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     4921 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     4981 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga 
     5041 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 
     5101 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 
     5161 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 
     5221 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 
     5281 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 
     5341 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 
     5401 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc 
     5461 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg 
     5521 ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga ggggcggggc 
     5581 ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct 
     5641 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga 
     5701 gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc 
     5761 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg 
     5821 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc 
     5881 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg 
     5941 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc 
     6001 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc 
     6061 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc 
     6121 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc 
     6181 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc 
     6241 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 
     6301 ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg 
     6361 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca 
     6421 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc 
     6481 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg 
     6541 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg 
     6601 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac 
     6661 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct 
     6721 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa 
//
