LOCUS       pAOX2BAX                8963 bp    DNA     circular     31-MAY-2010
DEFINITION  LMBP 6634.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6634
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 31-MAY-2010
            
            
            
            
COMMENT     VNTNAME|pAOX2BAX|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAOX2BAX" 0 8963 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 1 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "EcoRI" "EcoRV" "SacI" "SacII" "SphI" "XmnI" "PstI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 8963 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 8339 0 8805 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 8318 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 517 0 4342 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 8328 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 8961 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SphI" "gcatgc" 6 0 2 3549 0 8946 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 6 12 0 1460 0 2892 0 
COMMENT            3896 0 6291 0 6689 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 5937 0 8583 0 8940 0 0 "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "AOX1 terminator" 43 0 0 86 345 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "86..345") 
COMMENT        #9=(CFSignal (CObList) "pMB1 ori" 33 0 1 4675 5077 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4684..5086)") 
COMMENT        #10=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5618 6478 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5627..6487)") 
COMMENT        #11=(CFSignal (CObList) "mBax" 4 0 0 8353 8931 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "8362..8940") 
COMMENT        #12=(CFSignal (CObList) "AOX2 promoter" 29 0 0 6792 8316 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6801..8325") 
COMMENT        #13=(CFSignal (CObList) "Pichia HIS4" 4 0 1 745 3279 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2323..4857)") 
COMMENT        #14=(CFSignal (CObList) "3' flanking region of AOX1" 21 0 0 3634 4390 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5212..5968")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #15=(CFSignal (CObList) "LMBP 6634" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #16=(CFSignal (CObList) "Last modified: 31-MAY-2010" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                   0 4) 
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COMMENT                    (CObjectList 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pAOX2BAX'" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 6634." 
COMMENT                          1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 05/31/10 05:05" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8963 bp" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 16-MAR-2010" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 6634") 
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COMMENT                                 "" 1))) "Last modified: 31-MAY-2010"))) 
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COMMENT                                        "Start: 745   End: 3279 (Complementary)" 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2323..4857)" 1))) 
COMMENT                                 "Pichia HIS4") 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   complement(5627..6487)" 1))) 
COMMENT                                 "ampicillin resistance") 
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COMMENT                                        "Start: 8353  End: 8931" 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   8362..8940" 1))) "mBax"))) 
COMMENT                          "CDS (3 total)") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5212..5968" 1))) 
COMMENT                                 "3' flanking region of AOX1"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                        "Start: 6792  End: 8316" 1) 
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COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   6801..8325" 1))) "AOX2 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "Start: 4675  End: 5077 (Complementary)" 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4684..5086)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (1 total)") 
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COMMENT                                        "Start: 86    End: 345 " 1) 
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COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   86..345" 1))) "AOX1 terminator"))) 
COMMENT                          "Terminator (1 total)"))) "Feature Map") 
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COMMENT                                        " N1: 8318 " 1))))) "EcoRI: 1 site") 1 5 
COMMENT                          "GAATTC" "CTTAAG") 
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COMMENT                                        " N1: 8328 " 1))))) "SacI: 1 site") 5 1 
COMMENT                          "GAGCTC" "CTCGAG") 
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COMMENT                                        " N1: 8961 " 1))))) "SacII: 1 site") 4 2 
COMMENT                          "CCGCGG" "GGCGCC") 
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COMMENT                                        " N1: 8339 " 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 8805 " 1))))) "BamHI: 2 sites") 1 
COMMENT                          5 "GGATCC" "CCTAGG") 
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COMMENT                                        " N1: 517 " 1) 
COMMENT                                   #88=(CLinePar 
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COMMENT                                        " N2: 4342 " 1))))) "EcoRV: 2 sites") 3 
COMMENT                          3 "GATATC" "CTATAG") 
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COMMENT                                        " N1: 3549 " 1) 
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COMMENT                                        " N2: 8946 " 1))))) "SphI: 2 sites") 5 1 
COMMENT                          "GCATGC" "CGTACG") 
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COMMENT                                        " N2: 8583 " 1) 
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COMMENT                                        " N3: 8940 " 1))))) "PstI: 3 sites") 5 1 
COMMENT                          "CTGCAG" "GACGTC") 
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COMMENT                                         " N3: 2892 " 1) 
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COMMENT                                         " N6: 6689 " 1))))) "XmnI: 6 sites") 5 
COMMENT                          5 "GAANNNNTTC" "CTTNNNNAAG"))) 
COMMENT                   "Restriction/Methylation Map") 
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COMMENT                            "   Similarity between ambiguous nucleotides (if any)" 
COMMENT                            1) 
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COMMENT                            "is calculated as an AVERAGE similarity" 1))) 
COMMENT                    "Motifs (both strands)")))) 
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FEATURES             Location/Qualifiers
     terminator      86..345
                     /vntifkey="43"
                     /label=AOX1\terminator
     rep_origin      complement(4675..5077)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(5618..6478)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             8353..8931
                     /vntifkey="4"
                     /label=mBax
     promoter        6792..8316
                     /vntifkey="29"
                     /label=AOX2\promoter
     CDS             complement(745..3279)
                     /vntifkey="4"
                     /label=Pichia\HIS4
     misc_feature    3634..4390
                     /vntifkey="21"
                     /label=3'\flanking\region\of\AOX1
BASE COUNT     2386 a      2120 c      2047 g      2410 t 
ORIGIN
        1 ggccgcgaat taattcgcct tagacatgac tgttcctcag ttcaagttgg gcacttacga 
       61 gaagaccggt cttgctagat tctaatcaag aggatgtcag aatgccattt gcctgagaga 
      121 tgcaggcttc atttttgata cttttttatt tgtaacctat atagtatagg attttttttg 
      181 tcattttgtt tcttctcgta cgagcttgct cctgatcagc ctatctcgca gctgatgaat 
      241 atcttgtggt aggggtttgg gaaaatcatt cgagtttgat gtttttcttg gtatttccca 
      301 ctcctcttca gagtacagaa gattaagtga gaagttcgtt tgtgcaagct tatcgataag 
      361 ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa 
      421 tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc 
      481 ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc 
      541 agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt 
      601 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga 
      661 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcta tcgaatctaa 
      721 atgtaagtta aaatctctaa ataattaaat aagtcccagt ttctccatac gaaccttaac 
      781 agcattgcgg tgagcatcta gaccttcaac agcagccaga tccatcactg cttggccaat 
      841 atgtttcagt ccctcaggag ttacgtcttg tgaagtgatg aacttctgga aggttgcagt 
      901 gttaactccg ctgtattgac gggcatatcc gtacgttggc aaagtgtggt tggtaccgga 
      961 ggagtaatct ccacaactct ctggagagta ggcaccaaca aacacagatc cagcgtgttg 
     1021 tacttgatca acataagaag aagcattctc gatttgcagg atcaagtgtt caggagcgta 
     1081 ctgattggac atttccaaag cctgctcgta ggttgcaacc gatagggttg tagagtgtgc 
     1141 aatacacttg cgtacaattt caacccttgg caactgcaca gcttggttgt gaacagcatc 
     1201 ttcaattctg gcaagctcct tgtctgtcat atcgacagcc aacagaatca cctgggaatc 
     1261 aataccatgt tcagcttgag acagaaggtc tgaggcaacg aaatctggat cagcgtattt 
     1321 atcagcaata actagaactt cagaaggccc agcaggcatg tcaatactac acagggctga 
     1381 tgtgtcattt tgaaccatca tcttggcagc agtaacgaac tggtttcctg gaccaaatat 
     1441 tttgtcacac ttaggaacag tttctgttcc gtaagccata gcagctactg cctgggcgcc 
     1501 tcctgctagc acgatacact tagcaccaac cttgtgggca acgtagatga cttctggggt 
     1561 aagggtacca tccttcttag gtggagatgc aaaaacaatt tctttgcaac cagcaacttt 
     1621 ggcaggaaca cccagcatca gggaagtgga aggcagaatt gcggttccac caggaatata 
     1681 gaggccaact ttctcaatag gtcttgcaaa acgagagcag actacaccag ggcaagtctc 
     1741 aacttgcaac gtctccgtta gttgagcttc atggaatttc ctgacgttat ctatagagag 
     1801 atcaatggct ctcttaacgt tatctggcaa ttgcataagt tcctctggga aaggagcttc 
     1861 taacacaggt gtcttcaaag cgactccatc aaacttggca gttagttcta aaagggcttt 
     1921 gtcaccattt tgacgaacat tgtcgacaat tggtttgact aattccataa tctgttccgt 
     1981 tttctggata ggacgacgaa gggcatcttc aatttcttgt gaggaggcct tagaaacgtc 
     2041 aattttgcac aattcaatac gaccttcaga agggacttct ttaggtttgg attcttcttt 
     2101 aggttgttcc ttggtgtatc ctggcttggc atctcctttc cttctagtga cctttaggga 
     2161 cttcatatcc aggtttctct ccacctcgtc caacgtcaca ccgtacttgg cacatctaac 
     2221 taatgcaaaa taaaataagt cagcacattc ccaggctata tcttccttgg atttagcttc 
     2281 tgcaagttca tcagcttcct ccctaatttt agcgttcaac aaaacttcgt cgtcaaataa 
     2341 ccgtttggta taagaacctt ctggagcatt gctcttacga tcccacaagg tggcttccat 
     2401 ggctctaaga ccctttgatt ggccaaaaca ggaagtgcgt tccaagtgac agaaaccaac 
     2461 acctgtttgt tcaaccacaa atttcaagca gtctccatca caatccaatt cgatacccag 
     2521 caacttttga gttgctccag atgtagcacc tttataccac aaaccgtgac gacgagattg 
     2581 gtagactcca gtttgtgtcc ttatagcctc cggaatagac tttttggacg agtacaccag 
     2641 gcccaacgag taattagaag agtcagccac caaagtagtg aatagaccat cggggcggtc 
     2701 agtagtcaaa gacgccaaca aaatttcact gacagggaac tttttgacat cttcagaaag 
     2761 ttcgtattca gtagtcaatt gccgagcatc aataatgggg attataccag aagcaacagt 
     2821 ggaagtcaca tctaccaact ttgcggtctc agaaaaagca taaacagttc tactaccgcc 
     2881 attagtgaaa cttttcaaat cgcccagtgg agaagaaaaa ggcacagcga tactagcatt 
     2941 agcgggcaag gatgcaactt tatcaaccag ggtcctatag ataaccctag cgcctgggat 
     3001 catcctttgg acaactcttt ctgccaaatc taggtccaaa atcacttcat tgataccatt 
     3061 attgtacaac ttgagcaagt tgtcgatcag ctcctcaaat tggtcctctg taacggatga 
     3121 ctcaacttgc acattaactt gaagctcagt cgattgagtg aacttgatca ggttgtgcag 
     3181 ctggtcagca gcatagggaa acacggcttt tcctaccaaa ctcaaggaat tatcaaactc 
     3241 tgcaacactt gcgtatgcag gtagcaaggg aaatgtcata cttgaagtcg gacagtgagt 
     3301 gtagtcttga gaaattctga agccgtattt ttattatcag tgagtcagtc atcaggagat 
     3361 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc 
     3421 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc 
     3481 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc 
     3541 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg 
     3601 cttcctaatg caggagtcgc ataagggaga gcgtcgagta tctatgattg gaagtatggg 
     3661 aatggtgata cccgcattct tcagtgtctt gaggtctcct atcagattat gcccaactaa 
     3721 agcaaccgga ggaggagatt tcatggtaaa tttctctgac ttttggtcat cagtagactc 
     3781 gaactgtgag actatctcgg ttatgacagc agaaatgtcc ttcttggaga cagtaaatga 
     3841 agtcccacca ataaagaaat ccttgttatc aggaacaaac ttcttgtttc gaactttttc 
     3901 ggtgccttga actataaaat gtagagtgga tatgtcgggt aggaatggag cgggcaaatg 
     3961 cttaccttct ggaccttcaa gaggtatgta gggtttgtag atactgatgc caacttcagt 
     4021 gacaacgttg ctatttcgtt caaaccattc cgaatccaga gaaatcaaag ttgtttgtct 
     4081 actattgatc caagccagtg cggtcttgaa actgacaata gtgtgctcgt gttttgaggt 
     4141 catctttgta tgaataaatc tagtctttga tctaaataat cttgacgagc caaggcgata 
     4201 aatacccaaa tctaaaactc ttttaaaacg ttaaaaggac aagtatgtct gcctgtatta 
     4261 aaccccaaat cagctcgtag tctgatcctc atcaacttga ggggcactat cttgttttag 
     4321 agaaatttgc ggagatgcga tatcgagaaa aaggtacgct gattttaaac gtgaaattta 
     4381 tctcaagatc tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 
     4441 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 
     4501 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat 
     4561 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc 
     4621 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 
     4681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     4741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     4801 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     4861 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     4921 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     4981 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     5041 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     5101 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     5161 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     5221 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     5281 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     5341 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     5401 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     5461 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     5521 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     5581 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     5641 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     5701 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     5761 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     5821 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     5881 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca 
     5941 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     6001 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     6061 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     6121 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     6181 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg 
     6241 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     6301 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     6361 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     6421 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     6481 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     6541 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     6601 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 
     6661 tcacgaggcc ctttcgtctt caagaattaa ttctcatgtt tgacagctta tcatcgataa 
     6721 gctgactcat gttggtattg tgaaatagac gcagatcggg aacactgaaa aataacagtt 
     6781 attattcgag atcttttttt cagaccatat gaccggtcca tcttctacgg ggggattatc 
     6841 tatgctttga cctctatctt gattctttta tgattcaaat cacttttacg ttatttatta 
     6901 cttactggtt atttacttag cgccttttct gaaaaacatt tactaaaaat catacatcgg 
     6961 cactctcaaa cacgacagat tgtgatcaag aagcagagac aatcaccact aaggttgcac 
     7021 atttgagcca gtaggctcct aatagaggtt cgatacttat tttgataata cgacatattg 
     7081 tcttacctct gaatgtgtca atactctctc gttcttcgtc tcgtcagcta aaaatataac 
     7141 acttcgagta agatacgccc aattgaaggc tacgagatac cagactatca ctagtagaac 
     7201 tttgacatct gctaaagcag atcaaatatc catttatcca gaatcaatta ccttccttta 
     7261 gcttgtcgaa ggcatgaaaa agctacatga aaatccccat ccttgaagtt ttgtcagctt 
     7321 aaaggactcc atttcctaaa atttcaagca gtcctctcaa ctaaattttt ttccattcct 
     7381 ctgcacccag ccctcttcat caaccgtcca gccttctcaa aagtccaatg taagtagcct 
     7441 gcaaattcag gttacaaccc ctcaattttc catccaaggg cgatccttac aaagttaata 
     7501 tcgaacagca gagactaagc gagtcatcat caccacccaa cgatggtgaa aaactttaag 
     7561 catagattga tggagggtgt atggcacttg gcggctgcat tagagtttga aactatgggg 
     7621 taatacatca catccggaac tgatccgact ccgagatcat atgcaaagca cgtgatgtac 
     7681 cccgtaaact gctcggatta tcgttgcaat tcatcgtctt aaacagtaca agaaacttta 
     7741 ttcatgggtc attggactct gatgaggggc acatttcccc aatgattttt tgggaaagaa 
     7801 agccgtaaga ggacagttaa gcgaaagaga caagacaacg aacagcaaaa gtgacagctg 
     7861 tcagctacct agtggacagt tgggagtttc caattggttg gttttgaatt tttacccatg 
     7921 ttgagttgtc cttgcttctc cttgcaaaca atgcaagttg ataagacatc accttccaag 
     7981 ataggctatt tttgtcgcat aaatttttgt ctcggagtga aaaccccttt tatgtgaaca 
     8041 gattacagaa gcgtcctacc cttcaccggt tgagatgggg agaaaattaa gcgatgagga 
     8101 gacgattatt ggtataaaag aagcaaccaa aatcccttat tgtccttttc tgatcagcat 
     8161 caaagaatat tgtcttaaaa cgggctttta actacattgt tcttacacat tgcaaacctc 
     8221 ttccttctat ttcggatcaa ctgtattgac tacattgatc ttttttaacg aagtttacga 
     8281 cttactaaat ccccacaaac aaatcaactg agaaaagaat tcgagctcgg tacccgggga 
     8341 tcctctagag tcatggacgg gtccggggag cagcttggga gcggcgggcc caccagctct 
     8401 gaacagatca tgaagacagg ggcctttttg ctacagggtt tcatccagga tcgagcaggg 
     8461 aggatggctg gggagacacc tgagctgacc ttggagcagc cgccccagga tgcgtccacc 
     8521 aagaagctga gcgagtgtct ccggcgaatt ggagatgaac tggatagcaa tatggagctg 
     8581 cagaggatga ttgctgacgt ggacacggac tccccccgag aggtcttctt ccgggtggca 
     8641 gctgacatgt ttgctgatgg caacttcaac tggggccgcg tggttgccct cttctacttt 
     8701 gctagcaaac tggtgctcaa ggccctgtgc actaaagtgc ccgagctgat cagaaccatc 
     8761 atgggctgga cactggactt cctccgtgag cggctgcttg tctggatcca agaccagggt 
     8821 ggctgggaag gcctcctctc ctacttcggg acccccacat ggcagacagt gaccatcttt 
     8881 gtggctggag tcctcaccgc ctcgctcacc atctggaaga agatgggctg agacctgcag 
     8941 gcatgcaagc ttcgagccgc ggc 
//
