LOCUS       pBlue-loxP-PGK-gb2-hyg-loxP        4679 bp    DNA     circular     14-MAR-2014
DEFINITION  LMBP 6665.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6665
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 14-MAR-2014
COMMENT     VNTDATE|552066873|
COMMENT     VNTNAME|pBlue-loxP-PGK-gb2-hyg-loxP|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBlue-loxP-PGK-gb2-hyg-loxP" 0 4679 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 1 4289 4288 1 4679 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT            0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 875 0 1690 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 984 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1789 0 0 "")) 
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COMMENT        #6=(CFSignal (CObList) "synthetic polyA" 43 0 0 1677 1753 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1677..1753") 
COMMENT        #7=(CFSignal (CObList) "synthetic EM7 promoter" 30 0 0 565 630 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "565..630") 
COMMENT        #8=(CFSignal (CObList) "mPGK1 promoter" 29 0 0 42 552 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "42..552") 
COMMENT        #9=(CFSignal (CObList) "loxP" 21 0 0 7 40 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "7..40") 
COMMENT        #10=(CFSignal (CObList) "loxP" 21 0 0 1754 1787 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3021 3881 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3021..3881)") 
COMMENT        #12=(CFSignal (CObList) "f1 ori" 33 0 1 4012 4467 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "T7 promoter" 30 0 0 4635 4651 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "T3 promoter" 30 0 1 1823 1839 0 (CStringList) 
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COMMENT                                 "5' UTR of lacZ, incl. RBS"))) "RBS (1 total)") 
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FEATURES             Location/Qualifiers
     CDS             631..1656
                     /vntifkey="4"
                     /label=hygromycin\resistance
     terminator      1677..1753
                     /vntifkey="43"
                     /label=synthetic\polyA
     promoter        565..630
                     /vntifkey="30"
                     /label=synthetic\EM7\promoter
     promoter        42..552
                     /vntifkey="29"
                     /label=mPGK1\promoter
     misc_feature    7..40
                     /vntifkey="21"
                     /label=loxP
     misc_feature    1754..1787
                     /vntifkey="21"
                     /label=loxP
     CDS             complement(3021..3881)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(4012..4467)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        4635..4651
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(1823..1839)
                     /vntifkey="30"
                     /label=T3\promoter
     RBS             complement(1865..1902)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(1903..1986)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(2078..2480)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     1091 a      1219 c      1268 g      1101 t 
ORIGIN
        1 ggccgcataa cttcgtatag catacattat acgaagttat attctaccgg gtaggggagg 
       61 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct 
      121 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc 
      181 cgttctttgg tggccccgtc gcgccacctt ctactcctcc cctagtcagg aagttccccc 
      241 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc 
      301 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc 
      361 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg 
      421 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg 
      481 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg 
      541 gcctttcgac ctgcagcagc acgtgttgac aattaatcat cggcatagta tatcggcata 
      601 gtataatacg acaaggtgag gaactaaacc atgaaaaagc ctgaactcac cgcgacgtct 
      661 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag 
      721 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatacgt cctgcgggta 
      781 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc 
      841 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc 
      901 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct 
      961 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg 
     1021 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc 
     1081 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc 
     1141 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa 
     1201 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc 
     1261 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc 
     1321 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag 
     1381 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt 
     1441 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag 
     1501 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc 
     1561 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tacttgccga tagtggaaac 
     1621 cgacgcccca gcactcgtcc gagggcaaag gaataggttt ccctgccaca gtctgagagc 
     1681 tccctggcga attcggtacc aataaaagag ctttattttc atgatctgtg tgttggtttt 
     1741 tgtgtgcggc gcgataactt cgtatagcat acattatacg aagttatctc gagggggggc 
     1801 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg 
     1861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     1921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     1981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     2041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     2101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     2161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     2221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     2281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     2341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     2401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     2461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     2521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     2581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     2641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     2701 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     2761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     2821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     2881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     2941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     3001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     3061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     3121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     3181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     3241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     3301 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     3361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     3421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     3481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     3541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     3601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     3661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     3721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     3781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     3841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     3901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     3961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa 
     4021 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc 
     4081 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga 
     4141 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag 
     4201 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt 
     4261 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta 
     4321 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag 
     4381 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg 
     4441 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg 
     4501 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 
     4561 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 
     4621 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctccac cgcggtggc 
//
