ID pBlue-loxP-PGK-gb2-hyg-loxP standard; circular DNA; ; 4679 BP. DE LMBP 6665. CC Accession number: LMBP 6665 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 14-MAR-2014 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 631..1656 FT /vntifkey="4" FT /label=hygromycin\resistance FT terminator 1677..1753 FT /vntifkey="43" FT /label=synthetic\polyA FT promoter 565..630 FT /vntifkey="30" FT /label=synthetic\EM7\promoter FT promoter 42..552 FT /vntifkey="29" FT /label=mPGK1\promoter FT misc_feature 7..40 FT /vntifkey="21" FT /label=loxP FT misc_feature 1754..1787 FT /vntifkey="21" FT /label=loxP FT CDS complement(3021..3881) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(4012..4467) FT /vntifkey="33" FT /label=f1\ori FT promoter 4635..4651 FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(1823..1839) FT /vntifkey="30" FT /label=T3\promoter FT RBS complement(1865..1902) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT promoter complement(1903..1986) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin complement(2078..2480) FT /vntifkey="33" FT /label=pMB1\ori SQ Sequence 4679 BP; 1091 A; 1219 C; 1268 G; 1101 t; ggccgcataa cttcgtatag catacattat acgaagttat attctaccgg gtaggggagg 60 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct 120 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc 180 cgttctttgg tggccccgtc gcgccacctt ctactcctcc cctagtcagg aagttccccc 240 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc 300 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc 360 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg 420 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg 480 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg 540 gcctttcgac ctgcagcagc acgtgttgac aattaatcat cggcatagta tatcggcata 600 gtataatacg acaaggtgag gaactaaacc atgaaaaagc ctgaactcac cgcgacgtct 660 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag 720 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatacgt cctgcgggta 780 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc 840 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc 900 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct 960 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg 1020 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc 1080 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc 1140 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa 1200 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc 1260 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc 1320 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag 1380 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt 1440 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag 1500 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc 1560 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tacttgccga tagtggaaac 1620 cgacgcccca gcactcgtcc gagggcaaag gaataggttt ccctgccaca gtctgagagc 1680 tccctggcga attcggtacc aataaaagag ctttattttc atgatctgtg tgttggtttt 1740 tgtgtgcggc gcgataactt cgtatagcat acattatacg aagttatctc gagggggggc 1800 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg 1860 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 1920 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 1980 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 2040 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 2100 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 2160 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 2220 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 2280 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 2340 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 2400 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 2460 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 2520 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 2580 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 2640 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 2700 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 2760 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 2820 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 2880 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 2940 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 3000 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 3060 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 3120 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 3180 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 3240 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 3300 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 3360 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 3420 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 3480 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 3540 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 3600 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 3660 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 3720 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 3780 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 3840 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 3900 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 3960 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa 4020 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc 4080 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga 4140 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag 4200 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt 4260 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta 4320 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag 4380 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg 4440 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg 4500 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 4560 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 4620 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctccac cgcggtggc 4679 //