LOCUS       pAS2-mRIP1             10353 bp    DNA     circular     25-MAY-2010
DEFINITION  LMBP 6680.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6680
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 25-MAY-2010
            
            
            
COMMENT     VNTDATE|551025062|
COMMENT     VNTNAME|pAS2-mRIP1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAS2-mRIP1" 0 10353 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "BamHI" "BglII" "EcoRI" "NheI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CYC1 terminator" 
COMMENT             "ACAGGCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAG" 
COMMENT             "Fragment of pAS2; Length: 276  (laatste N: G (276) ipv A (275) -> midden HindIII)" 
COMMENT             0 (CStringList "terminator") 0)) 1 "{(0,1),2}" 0 0 "" 0 4294967295 
COMMENT        0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 3311 3586 1 10353 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #1=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 7959 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 5982 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BglII" "agatct" 2 0 1 3969 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 3951 0 5946 0 7607 0 0 
COMMENT            "") 
COMMENT        #5=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 6 615 0 1703 0 1782 0 
COMMENT            4302 0 6944 0 10036 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 9363 10223 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(9363..10223)") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 8420 8822 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(8420..8822)") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 1 8245 8328 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(8245..8328)") 
COMMENT        #9=(CFSignal (CObList) "ADH1 terminator" 43 0 0 7991 8092 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "7991..8092") 
COMMENT        #10=(CFSignal (CObList) "HA epitope" 22 0 0 5957 5983 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5957..5983") 
COMMENT        #11=(CFSignal (CObList) "ADH1 promoter" 29 0 0 5075 5478 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5075..5478") 
COMMENT        #12=(CFSignal (CObList) "CYH2" 4 0 1 3855 4304 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3855..4304)") 
COMMENT        #13=(CFSignal (CObList) "f1 ori" 33 0 1 2661 3116 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2661..3116)") 
COMMENT        #14=(CFSignal (CObList) "TRP1" 4 0 0 1884 2558 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1884..2558") 
COMMENT        #15=(CFSignal (CObList) "GALbd" 4 0 0 5501 5941 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5501..5941") 
COMMENT        #16=(CFSignal (CObList) "FLP terminator" 43 0 1 1060 1164 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1060..1164)") 
COMMENT        #17=(CFSignal (CObList) "2mu ori" 33 0 1 2 982 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2..982)") 
COMMENT        #18=(CFSignal (CObList) "CYC1 terminator" 43 0 1 3312 3586 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3311..3586)") 
COMMENT        #19=(CFSignal (CObList) "mRIP1" 4 0 0 5987 7957 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5987..7957")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #20=(CFSignal (CObList) "LMBP 6680" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #21=(CFSignal (CObList) "Last modified: 25-MAY-2010" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #23=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pAS2-mRIP1" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #24=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 1 178) 
COMMENT                    (CObjectList 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pAS2-mRIP1'" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 6680." 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 04/09/10 02:31" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 05/25/10 11:58" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 10353 bp" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #33=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 9-APR-2010" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #36=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #38=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #39=(CLinePar (CParagraph 0 (15 #20# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 6680") 
COMMENT                     #40=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #41=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 25-MAY-2010"))) 
COMMENT                   "Annotations") 
COMMENT              #42=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1884 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1884  End: 2558" 1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1884..2558" 1))) "TRP1") 
COMMENT                            #48=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3855 (3 #12# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3855  End: 4304 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3855..4304)" 1))) "CYH2") 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5501 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5501  End: 5941" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5501..5941" 1))) "GALbd") 
COMMENT                            #56=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5987 (3 #19# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5987  End: 7957" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5987..7957" 1))) "mRIP1") 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 9363 (3 #6# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 9363  End: 10223 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(9363..10223)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (5 total)") 
COMMENT                     #64=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #65=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5957 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5957  End: 5983" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5957..5983" 1))) "HA epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #69=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5075 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5075  End: 5478" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5075..5478" 1))) "ADH1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #74=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8245 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8245  End: 8328 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(8245..8328)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2     End: 982  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2..982)" 1))) "2mu ori") 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2661 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2661  End: 3116 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2661..3116)" 1))) 
COMMENT                                 "f1 ori") 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8420 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8420  End: 8822 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(8420..8822)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #92=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #93=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1060 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1060  End: 1164 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1060..1164)" 1))) 
COMMENT                                 "FLP terminator") 
COMMENT                            #97=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3312 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3312  End: 3586 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(3311..3586)" 1))) 
COMMENT                                 "CYC1 terminator") 
COMMENT                            #101=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 7991 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 7991  End: 8092" 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   7991..8092" 1))) "ADH1 terminator"))) 
COMMENT                          "Terminator (3 total)"))) "Feature Map") 
COMMENT              #105=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #106=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #107=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #108=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7959 " 1))))) "BamHI: 1 site") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #109=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #110=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #111=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5982 " 1))))) "NheI: 1 site") 
COMMENT                            1 5 "GCTAGC" "CGATCG") 
COMMENT                      #112=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #113=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #114=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3969 " 1))))) "BglII: 1 site") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
COMMENT                      #115=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #116=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #117=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3951 " 1) 
COMMENT                                      #118=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5946 " 1) 
COMMENT                                      #119=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 7607 " 1))))) "EcoRI: 3 sites") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #120=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #121=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #122=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 615 " 1) 
COMMENT                                      #123=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1703 " 1) 
COMMENT                                      #124=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1782 " 1) 
COMMENT                                      #125=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4302 " 1) 
COMMENT                                      #126=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 6944 " 1) 
COMMENT                                      #127=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 10036 " 1))))) "XmnI: 6 sites") 
COMMENT                            5 5 "GAANNNNTTC" "CTTNNNNAAG"))) 
COMMENT                    "Restriction/Methylation Map") 
COMMENT              #128=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 20 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #129=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 3092: pAS2-mRIP: XmnI(6944) - XmnI(10036)" 1) 4 
COMMENT                            "" 6944 10035) 
COMMENT                      #130=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 2642: pAS2-mRIP: XmnI(4302) - XmnI(6944)" 1) 4 "" 
COMMENT                            4302 6943) 
COMMENT                      #131=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 2520: pAS2-mRIP: XmnI(1782) - XmnI(4302)" 1) 4 "" 
COMMENT                            1782 4301) 
COMMENT                      #132=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 1088: pAS2-mRIP: XmnI(615) - XmnI(1703)" 1) 4 "" 
COMMENT                            615 1702) 
COMMENT                      #133=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 932: pAS2-mRIP: XmnI(10036) - XmnI(615)" 1) 4 "" 
COMMENT                            10036 614) 
COMMENT                      #134=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 79: pAS2-mRIP: XmnI(1703) - XmnI(1782)" 1) 4 "" 
COMMENT                            1703 1781))) "Restriction Fragments")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #135=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #136=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #137=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #138=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #139=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #140=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #141=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #142=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #143=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #144=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #145=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #146=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #147=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #148=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #149=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #150=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #151=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) #152=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #153=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #154=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #155=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #156=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #157=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #158=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #159=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #160=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #161=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #162=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #163=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #164=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #165=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #166=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #167=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #168=(CWidgetStyle "~Scrap" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #169=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711935 1 5 "@N (@S)" 0) 
COMMENT          #170=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #171=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #172=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 
COMMENT                1 5 "@N (@S)" 0) 
COMMENT          #173=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #174=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #175=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #176=(CWidgetStyle "Intron Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #177=(CWidgetStyle "Misc_Feature Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #178=(CWidgetStyle "Promoter_E Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #179=(CWidgetStyle "Promoter_P Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #180=(CWidgetStyle "Rep_Origin Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #181=(CWidgetStyle "Terminator Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #182=(CWidgetStyle "oriT Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #183=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #184=(CWidgetStyle "Intron" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #185=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #186=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #187=(CWidgetStyle "Label 8" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #188=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #189=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #190=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #191=(CWidgetStyle "Label 12" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #192=(CWidgetStyle "Uniq RSite" 1 (LOGPEN 0 0 16750899) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #193=(CWidgetStyle "Label 13" 1 (LOGPEN 0 0 16763904) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #194=(CWidgetStyle "Label 14" 1 (LOGPEN 0 0 39168) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #195=(CWidgetStyle "Label 15" 1 (LOGPEN 0 0 16750848) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #196=(CWidgetStyle "Marker" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #197=(CWidgetStyle "Marker Label" 1 (LOGPEN 0 0 0) 3 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #198=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 3 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #199=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #200=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 3381504) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #201=(CWidgetStyle "Label 16" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #202=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 16737792) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #203=(CWidgetStyle "Label 17" 1 (LOGPEN 0 0 16737792) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #204=(CWidgetStyle "Label 18" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #205=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #206=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #207=(CWidgetStyle "Label 19" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #208=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 153) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #209=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 10040064) 4 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #210=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #211=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #212=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #213=(CWidgetStyle "RBS Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #214=(CWidgetStyle "Label 22" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #215=(CWidgetStyle "Label 23" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #216=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #217=(CWidgetStyle "Attenuator" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #218=(CWidgetStyle "Shape 15" 1 (LOGPEN 0 0 6684774) 4 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #219=(CWidgetStyle "Promoter_P" 1 (LOGPEN 0 0 6684774) 8 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #220=(CWidgetStyle "Shape 16" 1 (LOGPEN 0 0 6684774) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #221=(CWidgetStyle "Shape 17" 1 (LOGPEN 0 0 0) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #222=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #223=(CWidgetStyle "Label 24" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #224=(CWidgetStyle "Label 25" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #225=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #226=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #227=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #228=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 16763955) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #229=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #230=(CWidgetStyle "Shape 21" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16763955 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #231=(CWidgetStyle "Attenuator Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #232=(CWidgetStyle "5'UTR Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #233=(CWidgetStyle "3'UTR Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #234=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #235=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #236=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #237=(CWidgetStyle "tRNA Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #238=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #239=(CWidgetStyle "Label 31" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #240=(CWidgetStyle "Label 32" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 
COMMENT                1 5 "@N (@S)" 0) 
COMMENT          #241=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #242=(CWidgetStyle "Label 33" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #243=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #244=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #245=(CWidgetStyle "Shape 30" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #246=(CWidgetStyle "Shape 31" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #247=(CWidgetStyle "Label 35" 1 (LOGPEN 0 0 3394560) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #248=(CWidgetStyle "Label 36" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #249=(CWidgetStyle "Label 37" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 8421376 1 5 "@N (@S)" 0) 
COMMENT          #250=(CWidgetStyle "Label 38" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #251=(CWidgetStyle "Label 39" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 65280 1 5 "@N (@S)" 0) 
COMMENT          #252=(CWidgetStyle "Label 40" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 8388736 1 5 "@N (@S)" 0) 
COMMENT          #253=(CWidgetStyle "Label 41" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711935 1 5 "@N (@S)" 0) 
COMMENT          #254=(CWidgetStyle "Label 42" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16776960 1 5 "@N (@S)" 0) 
COMMENT          #255=(CWidgetStyle "Label 43" 1 (LOGPEN 0 0 6723891) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 128 1 5 "@N (@S)" 0) 
COMMENT          #256=(CWidgetStyle "Label 44" 1 (LOGPEN 0 0 39321) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #257=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #258=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #259=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 21.4978 -16.157 40.3368 40.3368 0.01 10 -1 10353 10353 1 0 0) 
COMMENT        #260=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1701672291 100) 
COMMENT              (CObjectList 
COMMENT               #261=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -115 100) 
COMMENT                     (CObjectList 
COMMENT                      #262=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #151# 46353356 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27479 
COMMENT                             6.27279 1 0.0214037) 0.0605929) 
COMMENT                      #263=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #145# 46034444 100) 
COMMENT                            (LOGPEN 46336240 46034456 46034464) 46034468 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pAS2-mRIP1" "@N" 1 0 0.871165 0 -10 
COMMENT                            8.08195 1.56185 Nil) 
COMMENT                      #264=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #144# 4063768 100) 
COMMENT                            (LOGPEN 1 0 2081903016) 0 
COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "10353 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 5.107 1.80976 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #265=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -958 100) 
COMMENT                     (CObjectList 
COMMENT                      #266=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -1217 100) 
COMMENT                            (CObjectList 
COMMENT                             #267=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #147# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.0787708 0.600476 1 0.082322) 0.8 1.8 1) 
COMMENT                             #268=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #211# 2082602612 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ampicillin resistance" "@N" 1 -8.70266 
COMMENT                                   12.5612 0 -10 8.85048 1.11561 #267#) 
COMMENT                             #269=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #147# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    3.66527 3.93793 1 0.082322) 0.8 1.8 1) 
COMMENT                             #270=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #211# 4063648 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "CYH2" "@N" 1 
COMMENT                                   10.4337 -12.3133 0 -10 2.65266 1.11561 #269#) 
COMMENT                             #271=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #147# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.72322 5.13222 1 0.082322) 0.8 1.8 0) 
COMMENT                             #272=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #211# 44946740 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "TRP1" "@N" 1 
COMMENT                                   14.3802 3.47772 0 -10 2.57829 1.11561 #271#) 
COMMENT                             #273=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #147# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    2.67336 2.94057 1 0.082322) 0.8 1.8 0) 
COMMENT                             #274=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #211# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "GALbd" "@N" 1 
COMMENT                                   -6.35885 -13.8573 0 -10 3.19807 1.11561 #273#) 
COMMENT                             #275=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #19# 0) 1 2 0 0 #147# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    1.45181 2.64609 1 0.082322) 0.8 1.8 0) 
COMMENT                             #276=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #211# 539781490 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "mRIP1" "@N" 1 
COMMENT                                   -14.1804 -5.96379 0 -10 3.02453 1.11561 #275#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #277=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -753 100) 
COMMENT                            (CObjectList 
COMMENT                             #278=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #210# 1273 100) 
COMMENT                                   (LOGPEN 0 4 10040064) 6 1 2.65639 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #279=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #211# 4063768 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "HA epitope" 
COMMENT                                   "@N" 1 -8.72287 -11.1412 0 -10 5.00783 
COMMENT                                   1.11561 #278#)) (CObjectList)) 
COMMENT                      #280=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1144 100) 
COMMENT                            (CObjectList 
COMMENT                             #281=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #205# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    2.9539 3.1987 1 0.082322) 0.8 1.8 0) 
COMMENT                             #282=(CLabel (CWidget 0 (0 0) 1 2 0 0 #211# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ADH1 promoter" 
COMMENT                                   "@N" 1 -4.69539 -15.3022 0 -10 6.91676 
COMMENT                                   1.11561 #281#)) (CObjectList)) 
COMMENT                      #283=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -316 100) 
COMMENT                            (CObjectList 
COMMENT                             #284=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #157# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 1.2779 1.36517) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #285=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #211# 539781490 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 -14.797 5.344 0 -10 5.50366 1.11561 
COMMENT                                   #284#)) (CObjectList)) 
COMMENT                      #286=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -382 100) 
COMMENT                            (CObjectList 
COMMENT                             #287=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #146# 933 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    0.927678 1.17187 1 0.082322) 0.8 1.8 1) 
COMMENT                             #288=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #211# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -12.6496 6.888 0 -10 4.06577 1.11561 #287#) 
COMMENT                             #289=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #146# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    4.38511 4.66141 1 0.082322) 0.8 1.8 1) 
COMMENT                             #290=(CLabel (CWidget 0 (0 0) 1 2 0 0 #211# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 ori" "@N" 1 
COMMENT                                   14.4713 -3.10585 0 -10 2.2808 1.11561 #289#) 
COMMENT                             #291=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #146# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    5.67816 6.27258 1 0.082322) 0.8 1.8 1) 
COMMENT                             #292=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #211# 1094796097 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "2mu ori" "@N" 1 
COMMENT                                   5.54767 13.8573 0 -10 3.32203 1.11561 #291#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #293=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -168 100) 
COMMENT                            (CObjectList 
COMMENT                             #294=(CLine 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #155# 1269 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 1.37001 
COMMENT                                   1.43181) 
COMMENT                             #295=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #238# 942749493 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ADH1 terminator" "@N" 1 -16.0744 3.55209 0 
COMMENT                                   -10 7.363 1.11561 #294#) 
COMMENT                             #296=(CLine 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #155# 891 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 5.56788 
COMMENT                                   5.63151) 
COMMENT                             #297=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #238# 44964404 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "FLP terminator" 
COMMENT                                   "@N" 1 13.5171 11.7362 0 -10 6.54489 1.11561 
COMMENT                                   #296#) 
COMMENT                             #298=(CLine 
COMMENT                                   (CWidget 0 (3 #18# 0) 1 2 0 0 #155# 774 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 4.10032 
COMMENT                                   4.26695) 
COMMENT                             #299=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #238# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "CYC1 terminator" "@N" 1 15.1665 -6.9128 0 
COMMENT                                   -10 7.28863 1.11561 #298#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #300=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1028 100) 
COMMENT                     (CObjectList 
COMMENT                      #301=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -1079 100) 
COMMENT                            (CObjectList 
COMMENT                             #302=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #259# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 1.4512 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #303=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #243# 926234673 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Bam}HI (7959)" "@N (@S)" 5 -15.4576 
COMMENT                                   2.40475 0 -10 5.47887 1.06602 #302#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #304=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -1028 100) 
COMMENT                            (CObjectList 
COMMENT                             #305=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #259# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 2.64912 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #306=(CLabel (CWidget 0 (0 0) 1 2 0 0 #243# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Nhe}I (5982)" "@N (@S)" 5 -8.91545 
COMMENT                                   -9.89469 0 -10 4.68555 1.06602 #305#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #307=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -575 100) 
COMMENT                            (CObjectList 
COMMENT                             #308=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #259# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 3.86886 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #309=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #243# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Bgl}II (3969)" "@N (@S)" 5 12.0234 
COMMENT                                   -10.6206 0 -10 4.73513 1.06602 #308#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #310=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #4# 0) 1 2 0 0 Nil -941 100) 
COMMENT                            (CObjectList 
COMMENT                             #311=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #258# 2081903016 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 3.87976 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #312=(CLabel (CWidget 0 (0 0) 1 2 0 0 #257# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Eco}RI (3951)" "@N (@S)" 5 13.2687 
COMMENT                                   -9.07658 0 -10 5.23096 1.06602 #311#) 
COMMENT                             #313=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #258# 2081903016 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 2.67094 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #314=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 35491204 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Eco}RI (5946)" "@N (@S)" 5 -8.50207 
COMMENT                                   -12.5612 0 -10 5.23096 1.06602 #313#) 
COMMENT                             #315=(CScratch 
COMMENT                                   (CWidget 3 (1 #4# 3) 1 2 0 0 #258# 2081903016 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 1.66449 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #316=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Eco}RI (7607)" "@N (@S)" 5 -14.588 
COMMENT                                   -1.74233 0 -10 5.23096 1.06602 #315#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #317=(CGroupWidget 
COMMENT                            (CWidget 6 (10 #5# 0) 1 2 0 0 Nil -229 100) 
COMMENT                            (CObjectList 
COMMENT                             #318=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #258# 2081903016 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 5.90114 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #319=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Xmn}I (615)" "@N (@S)" 5 6.59643 
COMMENT                                   12.4373 0 -10 4.36326 1.06602 #318#) 
COMMENT                             #320=(CScratch 
COMMENT                                   (CWidget 2 (1 #5# 2) 1 2 0 0 #258# 45017516 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 5.24189 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #321=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 393233 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Xmn}I (1703)" "@N (@S)" 5 14.8863 
COMMENT                                   8.07798 0 -10 4.8095 1.06602 #320#) 
COMMENT                             #322=(CScratch 
COMMENT                                   (CWidget 3 (1 #5# 3) 1 2 0 0 #258# 45205380 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 5.19403 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #323=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 45171100 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Xmn}I (1782)" "@N (@S)" 5 14.888 
COMMENT                                   6.70752 0 -10 4.8095 1.06602 #322#) 
COMMENT                             #324=(CScratch 
COMMENT                                   (CWidget 4 (1 #5# 4) 1 2 0 0 #258# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.66708 1.9 0.082322 1) 
COMMENT                             #325=(CLabel (CWidget 0 (0 0) 1 2 0 0 #257# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Xmn}I (4302)" "@N (@S)" 5 10.4871 
COMMENT                                   -14.3036 0 -10 4.8095 1.06602 #324#) 
COMMENT                             #326=(CScratch 
COMMENT                                   (CWidget 5 (1 #5# 5) 1 2 0 0 #258# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 2.06622 1.9 0.082322 1) 
COMMENT                             #327=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 34043544 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Xmn}I (6944)" "@N (@S)" 5 -13.9442 
COMMENT                                   -7.21029 0 -10 4.8095 1.06602 #326#) 
COMMENT                             #328=(CScratch 
COMMENT                                   (CWidget 6 (1 #5# 6) 1 2 0 0 #258# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 0.192685 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #329=(CLabel (CWidget 0 (0 0) 1 2 0 0 #257# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Xmn}I (10036)" "@N (@S)" 5 -5.14878 
COMMENT                                   13.8821 0 -10 5.23096 1.06602 #328#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #330=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -530 100) 
COMMENT                     (CObjectList 
COMMENT                      #331=(CLabel 
COMMENT                            (CWidget 0 (15 #20# 0) 1 2 0 0 #135# 859391045 100) 
COMMENT                            (LOGPEN 893859373 875640375 944060470) 908935737 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 6680" 
COMMENT                            "LMBP 6680" 65535 0.247913 -2.52871 0 -10 7.41259 
COMMENT                            1.56185 Nil) 
COMMENT                      #332=(CLabel 
COMMENT                            (CWidget 0 (15 #21# 0) 1 2 0 0 #135# 539780469 100) 
COMMENT                            (LOGPEN 840989025 540029232 926104120) 1414350624 
COMMENT                            (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 25-MAY-2010" 
COMMENT                            "Last modified: 25-MAY-2010" 65535 0.371869 -3.89223 
COMMENT                            0 -10 11.3792 1.06602 Nil)) (CObjectList)) 
COMMENT               #333=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -114 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1196705607 
COMMENT       (CStringList) 1094795585 1195852099 
COMMENT       (CObList 
COMMENT        #334=(CSampFrag "pAS2-mRIP" 3092 6944 "XmnI" 10036 "XmnI" (LOGPEN 0 0 0) 
COMMENT              7.40475e+097 9.76133e-313) 
COMMENT        #335=(CSampFrag "pAS2-mRIP" 2642 4302 "XmnI" 6944 "XmnI" (LOGPEN 0 0 0) 
COMMENT              3.87206e+228 5.87938e-322) 
COMMENT        #336=(CSampFrag "pAS2-mRIP" 2520 1782 "XmnI" 4302 "XmnI" (LOGPEN 0 0 0) 
COMMENT              3.87206e+228 5.87938e-322) 
COMMENT        #337=(CSampFrag "pAS2-mRIP" 1088 615 "XmnI" 1703 "XmnI" (LOGPEN 0 0 0) 
COMMENT              2.25485e-316 1.02894e-314) 
COMMENT        #338=(CSampFrag "pAS2-mRIP" 932 10036 "XmnI" 615 "XmnI" (LOGPEN 0 0 0) 
COMMENT              3.03434e-086 9.76118e-313) 
COMMENT        #339=(CSampFrag "pAS2-mRIP" 79 1703 "XmnI" 1782 "XmnI" (LOGPEN 0 0 0) 
COMMENT              2.26488e-316 1.02894e-314))))
FEATURES             Location/Qualifiers
     CDS             complement(9363..10223)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(8420..8822)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(8245..8328)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      7991..8092
                     /vntifkey="43"
                     /label=ADH1\terminator
     misc_marker     5957..5983
                     /vntifkey="22"
                     /label=HA\epitope
     promoter        5075..5478
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             complement(3855..4304)
                     /vntifkey="4"
                     /label=CYH2
     terminator      complement(3312..3586)
                     /vntifkey="43"
                     /label=CYC1\terminator
     rep_origin      complement(2661..3116)
                     /vntifkey="33"
                     /label=f1\ori
     CDS             1884..2558
                     /vntifkey="4"
                     /label=TRP1
     CDS             5501..5941
                     /vntifkey="4"
                     /label=GALbd
     terminator      complement(1060..1164)
                     /vntifkey="43"
                     /label=FLP\terminator
     rep_origin      complement(2..982)
                     /vntifkey="33"
                     /label=2mu\ori
     CDS             5987..7957
                     /vntifkey="4"
                     /label=mRIP1
BASE COUNT     2973 a      2179 c      2366 g      2835 t 
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 
     5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 
     5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcatgc aaccagacat 
     6001 gtccttggac aatattaaga tggcatccag tgacctgctg gagaagacag acctagacag 
     6061 cggaggcttc gggaaggtgt ccttgtgtta ccacagaagc catggatttg tcatcctgaa 
     6121 aaaagtatac acagggccca accgcgctga gtacaatgag gttctcttgg aagaggggaa 
     6181 gatgatgcac agactgagac acagtcgagt ggtgaagcta ctgggcatca tcatagaaga 
     6241 agggaactat tcgctggtga tggagtacat ggagaagggc aacctgatgc acgtgctaaa 
     6301 gacccagata gatgtcccac tttcattgaa aggaaggata atcgtggagg ccatagaagg 
     6361 catgtgctac ttacatgaca aaggtgtgat acacaaggac ctgaagcctg agaatatcct 
     6421 cgttgatcgt gactttcaca ttaagatagc cgatcttggt gtggcttcct ttaagacatg 
     6481 gagcaaactg actaaggaga aagacaacaa gcagaaagaa gtgagcagca ccactaagaa 
     6541 gaacaatggt ggtacccttt actacatggc acccgaacac ctgaatgaca tcaatgcaaa 
     6601 gcccacggag aagtcggacg tgtacagctt tggcattgtc ctttgggcaa tatttgcaaa 
     6661 aaaggagccc tatgagaatg tcatctgtac tgagcagttc gtgatctgca taaaatctgg 
     6721 gaacaggcca aatgtagagg aaatccttga gtactgtcca agggagatca tcagcctcat 
     6781 ggagcggtgc tggcaggcga tcccagaaga caggccaaca tttcttggca ttgaagaaga 
     6841 atttaggcct ttttacttaa gtcattttga agaatatgta gaagaggatg tggcaagttt 
     6901 aaagaaagag tatccagatc aaagcccagt gctgcagaga atgttttcac tgcagcatga 
     6961 ctgtgtaccc ttacctccga gcaggtcaaa ttcagaacaa cctggatcgc tgcacagttc 
     7021 ccaggggctc cagatgggtc ctgtggagga gtcctggttt tcttcctccc cagagtaccc 
     7081 acaggacgag aatgatcgca gtgtgcaggc taagctgcaa gaggaagcca gctatcatgc 
     7141 ttttggaata tttgcagaga aacagacaaa accgcagcca aggcagaatg aggcttacaa 
     7201 cagagaggag gaaaggaaac gaagggtctc tcatgacccc tttgcacagc agagagctcg 
     7261 tgagaatatt aagagtgcag gagcaagagg tcattctgat cccagcacaa cgagtcgtgg 
     7321 aattgcagtg caacagctgt catggccagc cacccaaaca gtttggaaca atggattgta 
     7381 taatcagcat ggatttggaa ctacaggtac aggagtttgg tatccgccaa atctaagcca 
     7441 aatgtatagt acttataaaa ctccagtgcc agagaccaac ataccgggaa gcacacccac 
     7501 catgccatac ttctctgggc cagtagcaga tgacctcata aaatatacta tattcaatag 
     7561 ttctggtatt cagattggaa accacaatta tatggatgtt ggactgaatt cacaaccacc 
     7621 aaacaatact tgcaaagaag agtcgacttc cagacaccaa gccatctttg ataacaccac 
     7681 tagtctgact gatgaacacc tgaaccctat cagggaaaac ctgggaaggc agtggaaaaa 
     7741 ctgtgcccgc aagctgggct tcactgagtc tcagatcgat gaaatcgacc atgactatga 
     7801 aagagatgga ctgaaagaga aagtttacca aatgcttcag aagtggctga tgcgggaagg 
     7861 caccaaaggg gccacagtgg gaaagttggc ccaggcactt caccaatgtt gcaggataga 
     7921 cctgctgaac cacttgattc gtgccagcca gagctaagga tccgtcgacc tgcagccaag 
     7981 ctaattccgg gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa 
     8041 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttgttctt 
     8101 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat 
     8161 tgaccacacc tctaccggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg 
     8221 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 
     8281 gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct 
     8341 ttccagtcgg gaaacctgtc gtgccagctg gattaatgaa tcggccaacg cgcggggaga 
     8401 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     8461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     8521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     8581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     8641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     8701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     8761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     8821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     8881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     8941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     9001 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     9061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     9121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     9181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     9241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     9301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     9361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     9421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     9481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     9541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     9601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     9661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     9721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     9781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     9841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     9901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     9961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
    10021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
    10081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
    10141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
    10201 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
    10261 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
    10321 gttccgcgca catttccccg aaaagtgcca cct 
//
