ID pAS2-mRIP1 standard; circular DNA; ; 10353 BP. DE LMBP 6680. CC Accession number: LMBP 6680 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 25-MAY-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(9363..10223) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(8420..8822) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(8245..8328) FT /vntifkey="30" FT /label=lac\promoter FT terminator 7991..8092 FT /vntifkey="43" FT /label=ADH1\terminator FT misc_marker 5957..5983 FT /vntifkey="22" FT /label=HA\epitope FT promoter 5075..5478 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS complement(3855..4304) FT /vntifkey="4" FT /label=CYH2 FT terminator complement(3312..3586) FT /vntifkey="43" FT /label=CYC1\terminator FT rep_origin complement(2661..3116) FT /vntifkey="33" FT /label=f1\ori FT CDS 1884..2558 FT /vntifkey="4" FT /label=TRP1 FT CDS 5501..5941 FT /vntifkey="4" FT /label=GALbd FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT CDS 5987..7957 FT /vntifkey="4" FT /label=mRIP1 SQ Sequence 10353 BP; 2973 A; 2179 C; 2366 G; 2835 t; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2220 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2280 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2340 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2400 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2460 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2520 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2580 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2640 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2700 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2760 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2820 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2880 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2940 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3000 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3060 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3120 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3180 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3240 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3300 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3360 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3420 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3480 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3540 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3600 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3660 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3720 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3780 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3840 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3900 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3960 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4020 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4080 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4140 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4200 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4260 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4320 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4380 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4440 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4500 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4560 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4620 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4680 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4740 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4800 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4860 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4920 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4980 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5040 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5100 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5160 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5220 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 5280 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5340 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5400 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5460 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5520 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5580 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5640 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5700 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5760 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5820 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5880 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5940 gccggaattc atggcttacc catacgatgt tccagattac gctagcatgc aaccagacat 6000 gtccttggac aatattaaga tggcatccag tgacctgctg gagaagacag acctagacag 6060 cggaggcttc gggaaggtgt ccttgtgtta ccacagaagc catggatttg tcatcctgaa 6120 aaaagtatac acagggccca accgcgctga gtacaatgag gttctcttgg aagaggggaa 6180 gatgatgcac agactgagac acagtcgagt ggtgaagcta ctgggcatca tcatagaaga 6240 agggaactat tcgctggtga tggagtacat ggagaagggc aacctgatgc acgtgctaaa 6300 gacccagata gatgtcccac tttcattgaa aggaaggata atcgtggagg ccatagaagg 6360 catgtgctac ttacatgaca aaggtgtgat acacaaggac ctgaagcctg agaatatcct 6420 cgttgatcgt gactttcaca ttaagatagc cgatcttggt gtggcttcct ttaagacatg 6480 gagcaaactg actaaggaga aagacaacaa gcagaaagaa gtgagcagca ccactaagaa 6540 gaacaatggt ggtacccttt actacatggc acccgaacac ctgaatgaca tcaatgcaaa 6600 gcccacggag aagtcggacg tgtacagctt tggcattgtc ctttgggcaa tatttgcaaa 6660 aaaggagccc tatgagaatg tcatctgtac tgagcagttc gtgatctgca taaaatctgg 6720 gaacaggcca aatgtagagg aaatccttga gtactgtcca agggagatca tcagcctcat 6780 ggagcggtgc tggcaggcga tcccagaaga caggccaaca tttcttggca ttgaagaaga 6840 atttaggcct ttttacttaa gtcattttga agaatatgta gaagaggatg tggcaagttt 6900 aaagaaagag tatccagatc aaagcccagt gctgcagaga atgttttcac tgcagcatga 6960 ctgtgtaccc ttacctccga gcaggtcaaa ttcagaacaa cctggatcgc tgcacagttc 7020 ccaggggctc cagatgggtc ctgtggagga gtcctggttt tcttcctccc cagagtaccc 7080 acaggacgag aatgatcgca gtgtgcaggc taagctgcaa gaggaagcca gctatcatgc 7140 ttttggaata tttgcagaga aacagacaaa accgcagcca aggcagaatg aggcttacaa 7200 cagagaggag gaaaggaaac gaagggtctc tcatgacccc tttgcacagc agagagctcg 7260 tgagaatatt aagagtgcag gagcaagagg tcattctgat cccagcacaa cgagtcgtgg 7320 aattgcagtg caacagctgt catggccagc cacccaaaca gtttggaaca atggattgta 7380 taatcagcat ggatttggaa ctacaggtac aggagtttgg tatccgccaa atctaagcca 7440 aatgtatagt acttataaaa ctccagtgcc agagaccaac ataccgggaa gcacacccac 7500 catgccatac ttctctgggc cagtagcaga tgacctcata aaatatacta tattcaatag 7560 ttctggtatt cagattggaa accacaatta tatggatgtt ggactgaatt cacaaccacc 7620 aaacaatact tgcaaagaag agtcgacttc cagacaccaa gccatctttg ataacaccac 7680 tagtctgact gatgaacacc tgaaccctat cagggaaaac ctgggaaggc agtggaaaaa 7740 ctgtgcccgc aagctgggct tcactgagtc tcagatcgat gaaatcgacc atgactatga 7800 aagagatgga ctgaaagaga aagtttacca aatgcttcag aagtggctga tgcgggaagg 7860 caccaaaggg gccacagtgg gaaagttggc ccaggcactt caccaatgtt gcaggataga 7920 cctgctgaac cacttgattc gtgccagcca gagctaagga tccgtcgacc tgcagccaag 7980 ctaattccgg gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa 8040 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttgttctt 8100 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat 8160 tgaccacacc tctaccggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg 8220 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 8280 gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct 8340 ttccagtcgg gaaacctgtc gtgccagctg gattaatgaa tcggccaacg cgcggggaga 8400 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 8460 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 8520 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 8580 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 8640 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 8700 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 8760 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 8820 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 8880 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 8940 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 9000 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 9060 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 9120 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 9180 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 9240 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 9300 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 9360 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 9420 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 9480 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 9540 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 9600 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 9660 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 9720 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 9780 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 9840 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 9900 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 9960 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 10020 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 10080 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 10140 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 10200 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 10260 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 10320 gttccgcgca catttccccg aaaagtgcca cct 10353 //