LOCUS       pCD-F-bio-Ub-K33        5673 bp    DNA     circular     14-JAN-2010
DEFINITION  LMBP 6693.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|543163251|
COMMENT     VNTDBDATE|543163251|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCD-F-bio-Ub-K33|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Peter Marynen|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-F-bio-Ub-K33" 0 5673 
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COMMENT        (CHomObj 0 0 0 3 75) (CWordArray 84 27) (CWordArray) 
COMMENT        (CStringList "ApaI" "BamHI") (CStringList "atg") 
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COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5673 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 1243 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1001 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "atg" "atg" 1 0 87 29 0 58 0 168 0 211 0 300 0 
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COMMENT            0 3690 0 3859 0 4113 0 4301 0 4579 0 4626 0 4655 0 4683 0 4807 0 
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COMMENT            0 5523 0 5587 0 5607 0 0 "") 
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COMMENT            0 1290 0 1377 0 1382 0 1400 0 1466 0 1557 0 1696 0 1779 0 2041 0 
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COMMENT        #4=(CRSite (CStringList) "taa" "taa" 1 0 70 70 0 132 0 258 0 264 0 318 0 
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COMMENT        #5=(CRSite (CStringList) "tta" "tta" 1 0 80 69 0 131 0 181 0 187 0 243 0 
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COMMENT            5380 0 5444 0 5556 0 5568 0 5577 0 5613 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "tga" "tga" 1 0 94 56 0 110 0 154 0 169 0 234 0 
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COMMENT            0 5667 0 0 "") 
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COMMENT            0 5152 0 5156 0 5192 0 5233 0 5242 0 5290 0 5336 0 5339 0 5371 0 
COMMENT            5434 0 5450 0 5534 0 5550 0 5571 0 5585 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "tag" "tag" 1 0 60 66 0 114 0 182 0 188 0 251 0 
COMMENT            261 0 283 0 290 0 397 0 409 0 595 0 651 0 782 0 830 0 878 0 897 0 
COMMENT            1235 0 1279 0 1393 0 1459 0 1525 0 1549 0 1617 0 1704 0 1717 0 1754 
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COMMENT            0 4304 0 4353 0 4416 0 4449 0 4606 0 4736 0 4759 0 4941 0 4950 0 
COMMENT            4965 0 5100 0 5254 0 5316 0 5614 0 5630 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "cta" "cta" 1 0 61 27 0 136 0 250 0 440 0 502 0 
COMMENT            571 0 587 0 604 0 803 0 825 0 829 0 875 0 896 0 1005 0 1180 0 1278 0 
COMMENT            1357 0 1404 0 1488 0 1524 0 1599 0 1616 0 1685 0 1872 0 1882 0 1921 
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COMMENT            0 3994 0 4218 0 4313 0 4337 0 4341 0 4347 0 4352 0 4526 0 4605 0 
COMMENT            4644 0 4703 0 4720 0 4766 0 4919 0 4940 0 4991 0 0 "")) 
COMMENT       (CObList 
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COMMENT             (CStringList) 1 1 1 1 "209..863") 
COMMENT        #11=(CFSignal (CObList) "T7" 30 0 0 863 882 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "863..882") 
COMMENT        #12=(CFSignal (CObList) "BGH polyA" 25 0 0 1262 1476 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1262..1476") 
COMMENT        #13=(CFSignal (CObList) "f1" 33 0 0 1539 1952 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "SV40" 29 0 0 2017 2342 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "NeoR" 86 0 0 2378 3172 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2378..3172") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA" 25 0 0 3188 3427 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3188..3427") 
COMMENT        #17=(CFSignal (CObList) "ColE1" 33 0 0 3859 4532 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "AmpR" 22 0 1 4677 5538 0 (CStringList) 
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COMMENT                          "DNA 'pCD-F-bio-Ub-K33'" 1) 
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COMMENT                          "Currently local object. Original author: Peter Marynen" 
COMMENT                          1) 
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COMMENT                          "Created: 01/14/10 02:40" 1) 
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COMMENT                          "Last Modified: 01/14/10 02:40" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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FEATURES             Location/Qualifiers
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     polyA_signal    1262..1476
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      1539..1952
                     /vntifkey="33"
                     /label=f1
     promoter        2017..2342
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     2378..3172
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    3188..3427
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      3859..4532
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(4677..5538)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     primer          complement(1260..1276)
                     /vntifkey="27"
                     /label=1503
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     primer          947..982
                     /vntifkey="27"
                     /label=5925
     CDS             1007..1237
                     /vntifkey="4"
                     /label=Ub-K33
     misc_feature    947..1006
                     /vntifkey="21"
                     /label=bio-tag
     misc_feature    1022..1024
                     /vntifkey="21"
                     /label=R6
     misc_feature    1037..1039
                     /vntifkey="21"
                     /label=R11
     misc_feature    1085..1087
                     /vntifkey="21"
                     /label=R27
     misc_feature    1091..1093
                     /vntifkey="21"
                     /label=R29
     misc_feature    1148..1150
                     /vntifkey="21"
                     /label=R48
     misc_feature    1193..1195
                     /vntifkey="21"
                     /label=R63
     misc_feature    1103..1105
                     /vntifkey="21"
                     /label=K33
BASE COUNT     1319 a      1476 c      1460 g      1418 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac caggcctgaa 
      961 cgacatcttc gaggcccaga agatcgagtg gcacgaggag gatcctatgc agatcttcgt 
     1021 gaggaccctg accggcagga ccatcactct ggaggtggag cccagtgaca ccatcgaaaa 
     1081 tgtgagggcc agaatccaag ataaggaagg catccccccc gaccagcaga ggctcatctt 
     1141 tgcaggcagg cagctggaag atggccgcac tctttctgac tacaacatcc agagggagtc 
     1201 gaccctgcac ctggtcctgc gtctcagagg tgggtagggg cccgtttaaa cccgctgatc 
     1261 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 
     1321 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 
     1381 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 
     1441 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 
     1501 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 
     1561 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 
     1621 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 
     1681 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 
     1741 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 
     1801 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 
     1861 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc 
     1921 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 
     1981 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag 
     2041 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 
     2101 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 
     2161 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     2221 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 
     2281 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt 
     2341 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 
     2401 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 
     2461 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 
     2521 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 
     2581 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 
     2641 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 
     2701 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 
     2761 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 
     2821 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 
     2881 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 
     2941 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 
     3001 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 
     3061 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 
     3121 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 
     3181 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 
     3241 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 
     3301 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 
     3361 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     3421 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 
     3481 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 
     3541 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 
     3601 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 
     3661 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 
     3721 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 
     3781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 
     3841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 
     3901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 
     3961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 
     4021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 
     4081 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg 
     4141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 
     4201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 
     4261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 
     4321 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 
     4381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 
     4441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 
     4501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 
     4561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 
     4621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 
     4681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 
     4741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 
     4801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 
     4861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 
     4921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 
     4981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 
     5041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 
     5101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 
     5161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 
     5221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 
     5281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 
     5341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 
     5401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 
     5461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 
     5521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 
     5581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 
     5641 cgcacatttc cccgaaaagt gccacctgac gtc 
//
