ID pB2H-delta-alfa standard; circular DNA; ; 7549 BP. DE LMBP 6700. CC Accession number: LMBP 6700 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 13-JUN-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter complement(3154..3188) FT /vntifkey="30" FT /label=tac\promoter FT promoter complement(3295..3378) FT /vntifkey="30" FT /label=lac\promoter FT terminator 4003..4050 FT /vntifkey="43" FT /label=T7\terminator FT CDS complement(4270..4929) FT /vntifkey="4" FT /label=chloramphenicol\resistance FT promoter 7533..7549 FT /vntifkey="30" FT /label=T7\promoter FT rep_origin complement(5483..5981) FT /vntifkey="33" FT /label=p15A\ori FT CDS complement(75..3056) FT /vntifkey="4" FT /label=lacZ\delta-alfa\fragment FT promoter complement(7410..7487) FT /vntifkey="30" FT /label=lacI\promoter FT /note="E.coli repressor lacI promoter, incl. RBS" FT CDS complement(6327..7409) FT /vntifkey="4" FT /label=lacI FT RBS complement(3118..3153) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT promoter complement(3218..3234) FT /vntifkey="30" FT /label=SP6\promoter SQ Sequence 7549 BP; 1746 A; 2043 C; 1954 G; 1806 t; ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 60 gagatatacc atggttattt ttgacaccag accaactggt aatggtagcg accggcgctc 120 agctggaatt ccgccgatac tgacgggctc caggagtcgt cgccaccaat ccccatatgg 180 aaaccgtcga tattcagcca tgtgccttct tccgcgtgca gcagatggcg atggctggtt 240 tccatcagtt gctgttgact gtagcggctg atgttgaact ggaagtcgcc gcgccactgg 300 tgtgggccat aattcaattc gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg 360 tacggggtat acatgtctga caatggcaga tcccagcggt caaaacaggc ggcagtaagg 420 cggtcgggat agttttcttg cggccctaat ccgagccagt ttacccgctc tgctacctgc 480 gccagctggc agttcaggcc aatccgcgcc ggatgcggtg tatcgctcgc cacttcaaca 540 tcaacggtaa tcgccatttg accactacca tcaatccggt aggttttccg gctgataaat 600 aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa tcagcaccgc atcagcaagt 660 gtatctgccg tgcactgcaa caacgctgct tcggcctggt aatggcccgc cgccttccag 720 cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc 780 agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca gttgtttttt atcgccaatc 840 cacatctgtg aaagaaagcc tgactggcgg ttaaattgcc aacgcttatt acccagctcg 900 atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagc 960 gtcacactga ggttttccgc cagacgccac tgctgccagg cgctgatgtg cccggcttct 1020 gaccatgcgg tcgcgttcgg ttgcactacg cgtactgtga gccagagttg cccggcgctc 1080 tccggctgcg gtagttcagg cagttcaatc aactgtttac cttgtggagc gacatccaga 1140 ggcacttcac cgcttgccag cggcttacca tccagcgcca ccatccagtg caggagctcg 1200 ttatcgctat gacggaacag gtattcgctg gtcacttcga tggtttgccc ggataaacgg 1260 aactggaaaa actgctgctg gtgttttgct tccgtcagcg ctggatgcgg cgtgcggtcg 1320 gcaaagacca gaccgttcat acagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg 1380 ccgtaagccg accacgggtt gccgttttca tcatatttaa tcagcgactg atccacccag 1440 tcccagacga agccgccctg taaacgggga tactgacgaa acgcctgcca gtatttagcg 1500 aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc aaaggatcag cgggcgcgtc 1560 tctccaggta gcgaaagcca ttttttgatg gaccatttcg gcacagccgg gaagggctgg 1620 tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg tggccgtggt gtcggctccg 1680 ccgccttcat actgcaccgg gcgggaagga tcgacagatt tgatccagcg atacagcgcg 1740 tcgtgattag cgccgtggcc tgattcattc cccagcgacc agatgatcac actcgggtga 1800 ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga 1860 tcatcggtca gacgattcat tggcaccatg ccgtgggttt caatattggc ttcatccacc 1920 acatacaggc cgtagcggtc gcacagcgtg taccacagcg gatggttcgg ataatgcgaa 1980 cagcgcacgg cgttaaagtt gttctgcttc atcagcagga tatcctgcac catcgtctgc 2040 tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc 2100 aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca 2160 tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac caccgcacga 2220 tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag acgtagtgtg 2280 acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg cgcggtgccg 2340 ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta gtcacgcaac 2400 tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc attaaagcga 2460 gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga gacgtcacgg 2520 aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc actccaacgc 2580 agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca 2640 gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca cagatgaaac 2700 gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca 2760 acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa cggcggattg 2820 accgtaatgg gataggttac gttggtgtag atgggcgcat cgtaaccgtg catctgccag 2880 tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca gctttccggc 2940 accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct gcgcaactgt 3000 tgggaagggc gatcggtgcg ggcaacgacg gccagtgaat ccgtaatcat ggtcataccc 3060 ccggatccga attcgagctc ggcgcgcctg cagcggccgc atgcatatgg atcgatcctg 3120 tttcctgtgt gaaattgtta tccgctcaca attccacaca ttatacgagc cgatgattaa 3180 ttgtcaacag ggggatgggg agtaagcttg agtattctat agtgtcacct aaatagcttg 3240 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 3300 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 3360 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 3420 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 3480 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 3540 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgaat 3600 taattctcat gtttgacagc ttatcatcgg ataacccctt ggggcctcta aacgggtctt 3660 gaggggtttt ttgttctcat gtttgacagc ttatcttcga ttagctttaa tgcggtagtt 3720 tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca 3780 tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac 3840 tgccgggcct cttgcgggat atcggccggc cacgcgatcg ctgacgtcgg taccctcgag 3900 tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc acatggactc gtctactagc 3960 gcagcttaat taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg 4020 gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga gaagcacacg 4080 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt 4140 taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca 4200 tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc aataactgcc 4260 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat 4320 tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag 4380 caccttgtcg ccttgcgtat aatatttgcc catagtgaaa acgggggcga agaagttgtc 4440 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa 4500 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac 4560 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga 4620 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat 4680 caccagctca ccgtctttca ttgccatacg gaactccgga tgagcattca tcaggcgggc 4740 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa 4800 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc 4860 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt 4920 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg 4980 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca 5040 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta 5100 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc 5160 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt 5220 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg 5280 ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt 5340 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg 5400 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg 5460 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata 5520 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg 5580 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa 5640 gataccaggc gtttcccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt 5700 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt 5760 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc 5820 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac 5880 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt 5940 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg 6000 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg 6060 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat 6120 cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc 6180 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg 6240 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga 6300 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 6360 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc 6420 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg 6480 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt 6540 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact 6600 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc 6660 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc 6720 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga 6780 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa 6840 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg 6900 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag 6960 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg 7020 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc 7080 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc 7140 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga 7200 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg 7260 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa 7320 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct 7380 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg 7440 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg 7500 ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata 7549 //