LOCUS       pCAGGS-Flag-hIKKg        6115 bp    DNA     circular      2-DEC-2010
DEFINITION  LMBP 6761.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6761
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 2-DEC-2010
            
            
            
COMMENT     VNTDATE|572542239|
COMMENT     VNTNAME|pCAGGS-Flag-hIKKg|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-Flag-hIKKg" 0 6115 
COMMENT       (CDBMol 0 0 1 2433 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BanI" "HindIII" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6115 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 8 38 0 62 0 1969 0 3410 0 
COMMENT            5411 0 5632 0 5815 0 5859 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1867 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5 0 0 "")) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "intron AG promoter" 15 0 0 5140 6064 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "5129..6053") 
COMMENT        #4=(CFSignal (CObList) "AG promoter" 29 0 0 4771 6112 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4760..6101") 
COMMENT        #5=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4388 4765 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4377..4754") 
COMMENT        #6=(CFSignal (CObList) "FLAG epitope" 22 0 0 13 36 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "13..36") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 2446 2847 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2435..2836)") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2257 2331 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2246..2320)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2257 2331 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2246..2320") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 2151 2226 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2140..2215") 
COMMENT        #11=(CFSignal (CObList) "lac promoter" 30 0 1 1927 2009 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1916..1998)") 
COMMENT        #12=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 1493 1849 0 (CStringList) (CStringList) 1 1 1 1 "1482..1838") 
COMMENT        #13=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 1401 1492 0 (CStringList) (CStringList) 1 1 1 1 "1390..1481") 
COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3388 4248 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3377..4237)") 
COMMENT        #15=(CFSignal (CObList) "hIKKg" 4 0 0 54 1313 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "43..1302")) (CObList) (CObList) (CObList) 
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COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #17=(CFSignal (CObList) "LMBP 6761" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-Flag-hIKKg'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 12/02/10 03:30" 1) 
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COMMENT                          "Last Modified: 12/02/10 03:49" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6115 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 1-DEC-2010" 1))) 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     intron          5140..6064
                     /vntifkey="15"
                     /label=intron\AG\promoter
     promoter        4771..6112
                     /vntifkey="29"
                     /label=AG\promoter
     promoter        4388..4765
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
     rep_origin      complement(2446..2847)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2257..2331)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2257..2331
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      2151..2226
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1927..2009)
                     /vntifkey="30"
                     /label=lac\promoter
     misc_feature    1493..1849
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     terminator      1401..1492
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     CDS             complement(3388..4248)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             54..1313
                     /vntifkey="4"
                     /label=hIKKg
BASE COUNT     1312 a      1628 c      1794 g      1375 t         6 others
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccggtccgga attatgaata 
       61 ggcacctctg gaagagccaa ctgtgtgaga tggtgcagcc cagtggtggc ccggcagcag 
      121 atcaggacgt actgggcgaa gagtctcctc tggggaagcc agccatgctg cacctgcctt 
      181 cagaacaggg cgctcctgag accctccagc gctgcctgga ggagaatcaa gagctccgag 
      241 atgccatccg gcagagcaac cagattctgc gggagcgctg cgaggagctt ctgcatttcc 
      301 aagccagcca gagggaggag aaggagttcc tcatgtgcaa gttccaggag gccaggaaac 
      361 tggtggagag actcggcctg gagaagctcg atctgaagag gcagaaggag caggctctgc 
      421 gggaggtgga gcacctgaag agatgccagc agcagatggc tgaggacaag gcctctgtga 
      481 aagcccaggt gacgtccttg ctcggggagc tgcaggagag ccagagtcgc ttggaggctg 
      541 ccactaagga atgccaggct ctggagggtc gggcccgggc ggccagcgag caggcgcggc 
      601 agctggagag tgagcgcgag gcgctgcagc agcagcacag cgtgcaggtg gaccagctgc 
      661 gcatgcaggg ccagagcgtg gaggccgcgc tccgcatgga gcgccaggcc gcctcggagg 
      721 agaagaggaa gctggcccag ttgcaggtgg cctatcacca gctcttccaa gaatacgaca 
      781 accacatcaa gagcagcgtg gtgggcagtg agcggaagcg aggaatgcag ctggaagatc 
      841 tcaaacagca gctccagcag gccgaggagg ccctggtggc caaacaggag gtgatcgata 
      901 agctgaagga ggaggccgag cagcacaaga ttgtgatgga gaccgttccg gtgctgaagg 
      961 cccaggcgga tatctacaag gcggacttcc aggctgagag gcaggcccgg gagaagctgg 
     1021 ccgagaagaa ggagctcctg caggagcagc tggagcagct gcagagggag tacagcaaac 
     1081 tgaaggccag ctgtcaggag tcggccagga tcgaggacat gaggaagcgg catgtcgagg 
     1141 tctcccaggc ccccttgccc cccgcccctg cctacctctc ctctcccctg gccctgccca 
     1201 gccagaggag gagccccccc gaggagccac ctgacttctg ctgtcccaag tgccagtatc 
     1261 aggcccctga tatggacacc ctgcagatac atgtcatgga gtgcattgag tagnnnnnng 
     1321 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc 
     1381 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga 
     1441 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt 
     1501 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac 
     1561 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga 
     1621 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct 
     1681 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat 
     1741 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc 
     1801 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc 
     1861 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     1921 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag 
     1981 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 
     2041 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 
     2101 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     2161 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 
     2221 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat 
     2281 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 
     2341 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt 
     2401 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
     2461 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     2521 aggcggtaat acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa 
     2581 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     2641 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     2701 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     2761 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     2821 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     2881 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     2941 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     3001 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     3061 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     3121 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     3181 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     3241 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     3301 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     3361 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     3421 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     3481 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     3541 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     3601 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     3661 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 
     3721 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     3781 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
     3841 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
     3901 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
     3961 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 
     4021 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
     4081 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
     4141 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
     4201 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
     4261 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
     4321 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg 
     4381 gtcgatcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt 
     4441 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 
     4501 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca 
     4561 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca 
     4621 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 
     4681 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 
     4741 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc 
     4801 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt 
     4861 gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg gcggggcgag 
     4921 gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga 
     4981 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg 
     5041 cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc 
     5101 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct 
     5161 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg 
     5221 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt 
     5281 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt 
     5341 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg 
     5401 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg 
     5461 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc 
     5521 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg 
     5581 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc 
     5641 ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg gcccccggag 
     5701 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga 
     5761 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg 
     5821 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg 
     5881 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg 
     5941 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg 
     6001 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct 
     6061 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaa 
//
