LOCUS       pCAGGS-rtTA             5825 bp    DNA     circular     29-JUL-2009
DEFINITION  LMBP 6806.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|525128659|
COMMENT     VNTNAME|pCAGGS-rtTA|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     intron          4852..5776
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     promoter        4483..5824
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4100..4477
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     CDS             complement(3104..3964)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2162..2563)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      complement(1973..2047)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1973..2047
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      1867..1942
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        complement(1643..1725)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     misc_feature    1209..1565
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     terminator      1117..1208
                     /vntifkey="43"
                     /label=3'\UTR\of\ß-globin,\incl.\polyA
     CDS             10..1032
                     /vntifkey="4"
                     /label=rtTA
BASE COUNT     1280 a      1525 c      1599 g      1421 t 
ORIGIN
        1 aattccacca tgccaaagag acccagaccc tctagattag ataaaagtaa agtgattaac 
       61 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc 
      121 cagaagcttg gtgtagagca gcctacactg tattggcatg taaaaaataa gcgggctttg 
      181 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttaaaa 
      241 ggggaaagct ggcaagattt tttacgcaat aacgctaaaa gttttagatg tgctttacta 
      301 agtcatcgca atggagcaaa agtacattca gatacacggc ctacagaaaa acagtatgaa 
      361 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaacgcgtta 
      421 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat 
      481 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga 
      541 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa 
      601 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc gtacagccgc 
      661 gcgcgtacga aaaacaatta cgggtctacc atcgagggcc tgctcgatct cccggacgac 
      721 gacgcccccg aagaggcggg gctggcggct ccgcgcctgt cctttctccc cgcgggacac 
      781 acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc tgggggacga gctccactta 
      841 gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga tctggacatg 
      901 ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc cccctacggc 
      961 gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct tggaattgac 
     1021 gagtacggtg ggtaggaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct 
     1081 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat 
     1141 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt 
     1201 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg 
     1261 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat 
     1321 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc 
     1381 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata 
     1441 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta 
     1501 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg 
     1561 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 
     1621 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 
     1681 ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 
     1741 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc 
     1801 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
     1861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
     1921 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt 
     1981 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     2041 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     2101 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     2161 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     2221 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggat aacgcaggaa 
     2281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     2341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     2401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     2461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     2521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     2581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     2641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     2701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     2761 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 
     2821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     2881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     2941 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     3001 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     3061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     3121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     3181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     3241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     3301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     3361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     3421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     3481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     3541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     3601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     3661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     3721 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     3781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     3841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     3901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     3961 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     4021 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     4081 gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg 
     4141 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc 
     4201 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc 
     4261 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact 
     4321 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat 
     4381 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact 
     4441 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc 
     4501 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt 
     4561 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc aggcggggcg 
     4621 gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc caatcagagc 
     4681 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag 
     4741 cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc 
     4801 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg 
     4861 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct 
     4921 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc 
     4981 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg 
     5041 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc 
     5101 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa 
     5161 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg 
     5221 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg 
     5281 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg 
     5341 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga aggggcgcgg 
     5401 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg 
     5461 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc 
     5521 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag 
     5581 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct 
     5641 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg 
     5701 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt 
     5761 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg 
     5821 caaag 
//
