LOCUS       pACT2-IKKepsilon       10255 bp    DNA     circular     31-JUL-2015
DEFINITION  LMBP 6915.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|740919832|
COMMENT     VNTNAME|pACT2-IKKepsilon|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "EcoRI" "HindIII" "NcoI" "NdeI" "ScaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 7157 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NdeI" "catatg" 3 0 3 235 0 7165 0 8269 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 6892 0 9819 0 0 "")) 
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COMMENT        #6=(CFSignal (CObList) "FRT" 21 0 0 570 617 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "S. cerevisiae LEU2" 4 0 0 2474 3568 0 
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COMMENT        #217=(CSampFrag "pACT2-IKKepsilon" 7328 9819 "ScaI" 6892 "ScaI" 
COMMENT              (LOGPEN 0 0 0) 1.2239e-316 1.33409e-300) 
COMMENT        #218=(CSampFrag "pACT2-IKKepsilon" 2927 6892 "ScaI" 9819 "ScaI" 
COMMENT              (LOGPEN 0 0 0) 1.44956e-316 9.5261e-308))))
FEATURES             Location/Qualifiers
     terminator      418..522
                     /vntifkey="43"
                     /label=FLP\terminator
     misc_feature    570..617
                     /vntifkey="21"
                     /label=FRT
     rep_origin      600..1580
                     /vntifkey="33"
                     /label=2mu\ori
     CDS             2474..3568
                     /vntifkey="4"
                     /label=S.\cerevisiae\LEU2
     misc_feature    complement(4186..4219)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(4263..4296)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(4368..4401)
                     /vntifkey="21"
                     /label=loxP
     terminator      complement(4505..4606)
                     /vntifkey="43"
                     /label=ADH1\terminator
     CDS             complement(7219..7626)
                     /vntifkey="4"
                     /label=GALad
     promoter        complement(7637..8040)
                     /vntifkey="29"
                     /label=ADH1\promoter
     rep_origin      complement(8322..8724)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(9265..10125)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             complement(5003..7153)
                     /vntifkey="4"
                     /label=IKKepsilon
BASE COUNT     2815 a      2335 c      2365 g      2740 t 
ORIGIN
        1 gctttgaaga aaaatgcgcc ttattcaatc tttgctataa aaaatggccc aaaatctcac 
       61 attggaagac atttgatgac ctcatttctt tcaatgaagg gcctaacgga gttgactaat 
      121 gttgtgggaa attggagcga taagcgtgct tctgccgtgg ccaggacaac gtatactcat 
      181 cagataacag caatacctga tcactacttc gcactagttt ctcggtacta tgcatatgat 
      241 ccaatatcaa aggaaatgat agcattgaag gatgagacta atccaattga ggagtggcag 
      301 catatagaac agctaaaggg tagtgctgaa ggaagcatac gataccccgc atggaatggg 
      361 ataatatcac aggaggtact agactacctt tcatcctaca taaatagacg catataagta 
      421 cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat atacaggcaa 
      481 cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt 
      541 tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta 
      601 gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag acgcactttc 
      661 aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa taccgcttcc 
      721 acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc cctatataac 
      781 ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta cattttttat 
      841 gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat tcatagagtg 
      901 aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga gacaaaatag 
      961 aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca ctttctgttc 
     1021 acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt tatcttgaaa 
     1081 aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc aggctttttt 
     1141 tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga cctacagtgc 
     1201 aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag taatctaaga 
     1261 tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa aagaagtata 
     1321 gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct 
     1381 cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta caaaaatgaa 
     1441 gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc tgtaaaaatg 
     1501 cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttctacaaaa 
     1561 tgaagcacag atgcttcgtt aacaaagata tgctattgaa gtgcaagatg gaaacgcaga 
     1621 aaatgaaccg gggatgcgac gtgcaagatt acctatgcaa tagatgcaat agtttctcca 
     1681 ggaaccgaaa tacatacatt gtcttccgta aagcgctaga ctatatatta ttatacaggt 
     1741 tcaaatatac tatctgtttc agggaaaact cccaggttcg gatgttcaaa attcaatgat 
     1801 gggtaacaag tacgatcgta aatctgtaaa acagtttgtc ggatattagg ctgtatctcc 
     1861 tcaaagcgta ttcgaatatc attgagaagc tgcagcgtca catcggataa taatgatggc 
     1921 agccattgta gaagtgcctt ttgcatttct agtctctttc tcggtctagc tagttttact 
     1981 acatcgcgaa gatagaatct tagatcacac tgcctttgct gagctggatc aatagagtaa 
     2041 caaaagagtg gtaaggaact gtgggaatac tcaggtatcg taagatgcaa gagttcgaat 
     2101 ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg cgctatcgca 
     2161 cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc tgacgcatat 
     2221 acctttttca actgaaaaat tgggagaaaa aggaaaggtg agaggccgga accggctttt 
     2281 catatagaat agagaagcgt tcatgactaa atgcttgcat cacaatactt gaagttgaca 
     2341 atattattta aggacctatt gttttttcca ataggtggtt agcaatcgtc ttactttcta 
     2401 acttttctta ccttttacat ttcagcaata tatatatata tttcaaggat ataccattct 
     2461 aatgtctgcc cctatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg 
     2521 tcaagaaatc acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa 
     2581 tgtcaagttc gatttcgaaa atcatttaat tggtggtgct gctatcgatg ctacaggtgt 
     2641 cccacttcca gatgaggcgc tggaagcctc caagaaggtt gatgccgttt tgttaggtgc 
     2701 tgtgggtggt cctaaatggg gtaccggtag tgttagacct gaacaaggtt tactaaaaat 
     2761 ccgtaaagaa cttcaattgt acgccaactt aagaccatgt aactttgcat ccgactctct 
     2821 tttagactta tctccaatca agccacaatt tgctaaaggt actgacttcg ttgttgtcag 
     2881 agaattagtg ggaggtattt actttggtaa gagaaaggaa gacgatggtg atggtgtcgc 
     2941 ttgggatagt gaacaataca ccgttccaga agtgcaaaga atcacaagaa tggccgcttt 
     3001 catggcccta caacatgagc caccattgcc tatttggtcc ttggataaag ctaatgtttt 
     3061 ggcctcttca agattatgga gaaaaactgt ggaggaaacc atcaagaacg aatttcctac 
     3121 attgaaggtt caacatcaat tgattgattc tgccgccatg atcctagtta agaacccaac 
     3181 ccacctaaat ggtattataa tcaccagcaa catgtttggt gatatcatct ccgatgaagc 
     3241 ctccgttatc ccaggttcct tgggtttgtt gccatctgcg tccttggcct ctttgccaga 
     3301 caagaacacc gcatttggtt tgtacgaacc atgccacggt tctgctccag atttgccaaa 
     3361 gaataaggtt gaccctatcg ccactatctt gtctgctgca atgatgttga aattgtcatt 
     3421 gaacttgcct gaagaaggta aggccattga agatgcagtt aaaaaggttt tggatgcagg 
     3481 tatcagaact ggtgatttag gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc 
     3541 cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt tttttatgat atttgtacat 
     3601 aaactttata aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc 
     3661 atagggtaga cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga 
     3721 ggatagtaaa ggaatacagg taagcaaatt gatactaatg gctcaacgtg ataaggaaaa 
     3781 agaattgcac tttaacatta atattgacaa ggaggagggc accacacaaa aagttaggtg 
     3841 taacagaaaa tcatgaaact acgattccta atttgatatt ggaggatttt ctctaaaaaa 
     3901 aaaaaaatac aacaaataaa aaacactcaa tgacctgacc atttgatgga gtttaagtca 
     3961 ataccttctt gaaccatttc ccataatggt gaaagttccc tcaagaattt tactctgtca 
     4021 gaaacggcct taagcacgta gtgcaccaat tccgatcata tcaataaccc ttaatataac 
     4081 ttcgtataat gtatgctatc gaagttatta ggtctgaaga ggagtttacg tccagccaag 
     4141 ctagcctggc tgcaggtcga gcggccgcga tccggaaccc ttaatataac ttcgtataat 
     4201 gtatgctata cgaagttatt aggtccctcg accaattccg atcatattca ataaccctta 
     4261 atataacttc gtataatgta tgctatacga agttattagg tctgaagagg agtttacgtc 
     4321 cgctagcttg gctgcaggtc gagcggccgc gatccggaac ccttaatata acttcgtata 
     4381 atgtatgcta tacgaagtta ttaggtccct cgcacatgcc ggtagaggtg tggtcaataa 
     4441 gagcgacctc atgctatacc tgagaaagca acctgaccta caggaaagag ttactcaaga 
     4501 acaagaattt tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt 
     4561 ttataactta tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgt 
     4621 caacaacgta tctaccaacg atttgaccct tttccatctt ttcgtaaatt tctggcaagg 
     4681 tagacaagcc gacaaccttg attggagact tgaccaaacc tctggcgaag aagtccaaag 
     4741 cttctgaata agccctcgta atatattttc atgaagaatt taggtccaaa aaaaagatgg 
     4801 gcattaattc tagtcattta aaaaattcta tagatcagag gttacatggc caagattgaa 
     4861 acttagagga gtatagttac ataaaagaag gcaaaacgat gtataaatga aagaaattga 
     4921 gatggtgcac gatgcacagt tgaagtgaac ttgcggggtt tttcagtatc tacgattcat 
     4981 agatctctcg agctcgaatt cgtcagacat caggaggtgc tgggactcta tttagccgtt 
     5041 cgatgatgcg gttgttgtcc aggaggtcag atgccagcag cttcatcccc tcgcagagct 
     5101 cttgcatgtg gagaagcagg tcctttcgtg tagggctggg gtaaggagct atgggaggcg 
     5161 gcgaggcctg agcccccttt gctcggtcct gaaggagctg gtgagatagc tcttccagga 
     5221 gcttgctgag actctcctgg accccctggg cttctgtgtt acaggcagcc acagaacagc 
     5281 caaccaggcg caggtggttc ctggtctcgt gcaccaccct catcctcttg ccgtgtgtga 
     5341 ctaaggacgc ttgatacttc tgcacgcact cctcctggaa cacctgcagg agtcttttgg 
     5401 ctaaatgact gaaattcacc ttatccagct tgtgaatctg ctcctcgttg tagccaagcc 
     5461 ctggcctcat cctagacttc ttgaactgtt tgtagatgaa gttcatcttg tccaaacagc 
     5521 actgaatctg ctggatgctt ctgtcctcat gtacctgatc ccggctcttc accagctccc 
     5581 ggttcaggct gctcaggctc tcctgggtct ccgtgatatt ttgggagcat ctggagagga 
     5641 cctccgctag agtccgcagc ctggacctca gttctgcagc cgccttcagt tcctggatct 
     5701 caggcgttcc agccacgctg ctgaacctct cagttcccag gctgctgctg aggtagagga 
     5761 gggatgtcct tgccacttcc agagtccgtc tgcatgtggc ctgtagcacc tccatgaccc 
     5821 agtgcagccc ccgaaacatt agctcctgcc catccagcag ggcccgtgcc agccgcaggg 
     5881 cctggtagcc ggcgcccaac acgcccttgg cagtgttgta atccgcctgc aggtccactt 
     5941 tggggacgaa cttggggacg tccagagcag ggtccctgaa ggccagcccc ttagggatgg 
     6001 ctgtgctgaa gagggtcagg gggctgcttg ccgtcgtgtg ggcgatgtgc tgtgctgaga 
     6061 cgctgggctc gaggacacag aggtgaccct caaagaggta ctcctggtgt cggggggcca 
     6121 cactggtctg cttgtgcacg gcctcctgga aaatggctat cgtgttgtgg gcatggatat 
     6181 agatgtggtg caggactgcc tgggacaggg agaagacatg gacgacaact cgctgcagga 
     6241 tgtcactggt ctccgcaaag aactggtcga agccccagca cttggcctgc tccacctcca 
     6301 ggatgttggc caggatgggc accagctggc tctgcagccc cagtgacagc tggcaggtga 
     6361 tggggagggt gtagctccac tccaggggcc cgttctcccg cctctgggca cctgcaatgg 
     6421 ccccagccgg cttctccgtg gtgatccggt acatgatctc cttgttccgc cgtggcccac 
     6481 caaaggggat gaagggcagg ctgccagtgg ctgcatggta caaggtcact ccaatgctcc 
     6541 agagatccac agtcaccccg aacgcttttt gctggggctt tcgaagcacc gcccgctcat 
     6601 acatgtcggg atgcaggtac tcctcagtcc catagaccga gacgaacttc tcatcatcat 
     6661 ccagctcccg ggcagcgccg aagtctgtca gcttgtagat gctctgcccc tcctccccta 
     6721 cgaggcgcat gatgttcccc ggcttgatgt cgcgatgcac aatgccgttc tcccgcaggt 
     6781 ggttcatgcc ggccaccaca cagcgcagca ccaccaggaa ctcatcctca ggcagcccaa 
     6841 aggcattctc agggctctcc agcacactca gcaggctccc actggagcag tactccatca 
     6901 ccagtacctt ctgccggctt ccgcccgtct cctccaccgc aaagagcttg atgatgttct 
     6961 ggtggttcag cttccgcagg acctcaaact ccctcacctg cacctcgcgg ggccgcaggt 
     7021 agctggtagt gttgaagacc ttcacagcaa ccagctctcc ggatttcttg ttgcgggcct 
     7081 tgtacacact ggcagtggcc ccctgcccca gcaggtcatc tgtgtgccac aggtaattgg 
     7141 ctgtgctctg catctccatg gccatatgac cacccaagtt agcggaatct ggaacatcgt 
     7201 atgggtaagc catagagact ctttttttgg gtttggtggg gtatcttcat catcgaatag 
     7261 atagttatat acatcatcca ttgtagtggt attaaacatc cctgtagtga ttccaaacgc 
     7321 gttatacgca gtttggtccg tccaaccagg tgacagtggt tttgaattat taccatcatc 
     7381 aattttacta gccgtgattt cattattcat gaagttatca tgaacgttag aggaggcaat 
     7441 tggttgtgaa agcgcttgag aatttgtttg agttgttatg aggttcggac cgttgctact 
     7501 gttagtgaaa gtgaaggaca atgagctatc agcaatattc ccactttgat taaaattggc 
     7561 ggcggtaccc aattcgacct ttctcttctt ttttggaggc tcgggaatta attccgcttt 
     7621 atccatcttt gcaaagcttg gagttgattg tatgcttggt atagcttgaa atattgtgca 
     7681 gaaaaagaaa caaggaagaa agggaacgag aacaatgacg aggaaacaaa agattaataa 
     7741 ttgcaggtct atttatactt gatagcaaag cggcaaactt tttttatttc aaattcaagt 
     7801 aactggaagg aaggccgtat accgttgctc attagagagt agtgtgcgtg aatgaaggaa 
     7861 ggaaaaagtt tcgtgtgttc gaagataccc ctcatcagct ctggaacaac gacatctgtt 
     7921 ggtgctgtct ttgtcgttaa ttttttcctt tagtgtcttc catcattttt tttgtcattg 
     7981 cggatatggt gagacaacaa cgggggagag agaaaagaaa aaaaaagaaa agaagttgct 
     8041 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 
     8101 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 
     8161 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 
     8221 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 
     8281 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 
     8341 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     8401 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     8461 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     8521 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     8581 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     8641 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     8701 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     8761 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     8821 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     8881 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     8941 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     9001 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     9061 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     9121 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     9181 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     9241 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     9301 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     9361 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     9421 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     9481 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     9541 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg 
     9601 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     9661 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     9721 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     9781 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     9841 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc 
     9901 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     9961 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
    10021 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
    10081 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
    10141 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
    10201 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacct 
//
