LOCUS       pAS2-IKKepsilon        10558 bp    DNA     circular      3-APR-2015
DEFINITION  LMBP 6916.
SOURCE      
  ORGANISM  
COMMENT     Accession number: 6916
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 3-APR-2015
            
COMMENT     VNTDATE|582653553|
COMMENT     VNTNAME|pAS2-IKKepsilon|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
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COMMENT        #3=(CFSignal (CObList) "f1 ori" 33 0 1 2661 3116 0 (CStringList) 
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COMMENT        #4=(CFSignal (CObList) "TRP1" 4 0 0 1884 2558 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1884..2558") 
COMMENT        #5=(CFSignal (CObList) "2mu ori" 33 0 1 2 982 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 9568 10428 0 
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COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 8625 9027 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pAS2-IKKepsilon'" 1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
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FEATURES             Location/Qualifiers
     CDS             complement(3855..4304)
                     /vntifkey="4"
                     /label=CYH2
     rep_origin      complement(2661..3116)
                     /vntifkey="33"
                     /label=f1\ori
     CDS             1884..2558
                     /vntifkey="4"
                     /label=TRP1
     rep_origin      complement(2..982)
                     /vntifkey="33"
                     /label=2mu\ori
     CDS             complement(9568..10428)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(8625..9027)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      8196..8297
                     /vntifkey="43"
                     /label=ADH1\terminator
     misc_marker     5957..5983
                     /vntifkey="22"
                     /label=HA\epitope
     CDS             5501..5941
                     /vntifkey="4"
                     /label=GALbd
     promoter        5075..5478
                     /vntifkey="29"
                     /label=ADH1\promoter
     terminator      complement(3312..3586)
                     /vntifkey="43"
                     /label=CYC1\terminator
     promoter        complement(8450..8533)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      complement(1060..1164)
                     /vntifkey="43"
                     /label=FLP\terminator
     CDS             6011..8161
                     /vntifkey="4"
                     /label=IKBKE
BASE COUNT     2835 a      2366 c      2543 g      2814 t 
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 
     5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 
     5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 
     6001 ggccatggag atgcagagca cagccaatta cctgtggcac acagatgacc tgctggggca 
     6061 gggggccact gccagtgtgt acaaggcccg caacaagaaa tccggagagc tggttgctgt 
     6121 gaaggtcttc aacactacca gctacctgcg gccccgcgag gtgcaggtga gggagtttga 
     6181 ggtcctgcgg aagctgaacc accagaacat catcaagctc tttgcggtgg aggagacggg 
     6241 cggaagccgg cagaaggtac tggtgatgga gtactgctcc agtgggagcc tgctgagtgt 
     6301 gctggagagc cctgagaatg cctttgggct gcctgaggat gagttcctgg tggtgctgcg 
     6361 ctgtgtggtg gccggcatga accacctgcg ggagaacggc attgtgcatc gcgacatcaa 
     6421 gccggggaac atcatgcgcc tcgtagggga ggaggggcag agcatctaca agctgacaga 
     6481 cttcggcgct gcccgggagc tggatgatga tgagaagttc gtctcggtct atgggactga 
     6541 ggagtacctg catcccgaca tgtatgagcg ggcggtgctt cgaaagcccc agcaaaaagc 
     6601 gttcggggtg actgtggatc tctggagcat tggagtgacc ttgtaccatg cagccactgg 
     6661 cagcctgccc ttcatcccct ttggtgggcc acggcggaac aaggagatca tgtaccggat 
     6721 caccacggag aagccggctg gggccattgc aggtgcccag aggcgggaga acgggcccct 
     6781 ggagtggagc tacaccctcc ccatcacctg ccagctgtca ctggggctgc agagccagct 
     6841 ggtgcccatc ctggccaaca tcctggaggt ggagcaggcc aagtgctggg gcttcgacca 
     6901 gttctttgcg gagaccagtg acatcctgca gcgagttgtc gtccatgtct tctccctgtc 
     6961 ccaggcagtc ctgcaccaca tctatatcca tgcccacaac acgatagcca ttttccagga 
     7021 ggccgtgcac aagcagacca gtgtggcccc ccgacaccag gagtacctct ttgagggtca 
     7081 cctctgtgtc ctcgagccca gcgtctcagc acagcacatc gcccacacga cggcaagcag 
     7141 ccccctgacc ctcttcagca cagccatccc taaggggctg gccttcaggg accctgctct 
     7201 ggacgtcccc aagttcgtcc ccaaagtgga cctgcaggcg gattacaaca ctgccaaggg 
     7261 cgtgttgggc gccggctacc aggccctgcg gctggcacgg gccctgctgg atgggcagga 
     7321 gctaatgttt cgggggctgc actgggtcat ggaggtgcta caggccacat gcagacggac 
     7381 tctggaagtg gcaaggacat ccctcctcta cctcagcagc agcctgggaa ctgagaggtt 
     7441 cagcagcgtg gctggaacgc ctgagatcca ggaactgaag gcggctgcag aactgaggtc 
     7501 caggctgcgg actctagcgg aggtcctctc cagatgctcc caaaatatca cggagaccca 
     7561 ggagagcctg agcagcctga accgggagct ggtgaagagc cgggatcagg tacatgagga 
     7621 cagaagcatc cagcagattc agtgctgttt ggacaagatg aacttcatct acaaacagtt 
     7681 caagaagtct aggatgaggc cagggcttgg ctacaacgag gagcagattc acaagctgga 
     7741 taaggtgaat ttcagtcatt tagccaaaag actcctgcag gtgttccagg aggagtgcgt 
     7801 gcagaagtat caagcgtcct tagtcacaca cggcaagagg atgagggtgg tgcacgagac 
     7861 caggaaccac ctgcgcctgg ttggctgttc tgtggctgcc tgtaacacag aagcccaggg 
     7921 ggtccaggag agtctcagca agctcctgga agagctatct caccagctcc ttcaggaccg 
     7981 agcaaagggg gctcaggcct cgccgcctcc catagctcct taccccagcc ctacacgaaa 
     8041 ggacctgctt ctccacatgc aagagctctg cgaggggatg aagctgctgg catctgacct 
     8101 cctggacaac aaccgcatca tcgaacggct aaatagagtc ccagcacctc ctgatgtctg 
     8161 agggatccgt cgacctgcag ccaagctaat tccgggcgaa tttcttatga tttatgattt 
     8221 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg 
     8281 ttttaaaacg aaaattcttg ttcttgagta actctttcct gtaggtcagg ttgctttctc 
     8341 aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgcaa gcttggcgta 
     8401 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 
     8461 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt 
     8521 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctggatta 
     8581 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 
     8641 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     8701 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     8761 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     8821 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     8881 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     8941 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     9001 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     9061 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     9121 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     9181 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     9241 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     9301 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     9361 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     9421 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     9481 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     9541 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     9601 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     9661 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     9721 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     9781 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     9841 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 
     9901 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     9961 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
    10021 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
    10081 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
    10141 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 
    10201 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
    10261 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
    10321 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
    10381 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
    10441 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
    10501 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacct 
//
