LOCUS       pCAGGS-FLAG-MDA5-CARD        5489 bp    DNA     circular     11-SEP-2014
DEFINITION  LMBP 6920.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6920
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-SEP-2014
COMMENT     VNTDATE|707390972|
COMMENT     VNTNAME|pCAGGS-FLAG-MDA5-CARD|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-FLAG-MDA5-CARD" 0 5489 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "HincII" "KpnI" "PvuI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 1 0 0 0 0 1 5489 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 766 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HincII" "gtyrac" 4 0 2 465 0 624 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 33 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 3197 0 3752 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5485 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "intron AG promoter" 15 0 0 4505 5429 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4502..5426") 
COMMENT        #6=(CFSignal (CObList) "AG promoter" 29 0 0 4136 5477 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4133..5474") 
COMMENT        #7=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3753 4130 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3750..4127") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2753 3613 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2750..3610)") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 1292 1374 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1289..1371)") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 1516 1591 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1513..1588") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1622 1696 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1619..1693") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1622 1696 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1619..1693)") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 1811 2212 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1808..2209)") 
COMMENT        #14=(CFSignal (CObList) "hIFIH1 CARD" 4 0 0 34 753 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "34..753") 
COMMENT        #15=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 766 857 0 (CStringList) (CStringList) 1 1 1 1 "763..854") 
COMMENT        #16=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 858 1214 0 (CStringList) (CStringList) 1 1 1 1 "855..1211") 
COMMENT        #17=(CFSignal (CObList) "FLAG epitope" 22 0 0 4 27 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4..27")) (CObList) (CObList) (CObList) 
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COMMENT        #18=(CFSignal (CObList) "LMBP 6920" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 11-SEP-2014" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-FLAG-MDA5-CARD'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: Geert van Loo" 
COMMENT                          1) 
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COMMENT                          "Created: 08/27/14 09:29" 1) 
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COMMENT                          "Last Modified: 09/11/14 01:42" 1) 
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COMMENT                          "length: 5489 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 27-AUG-2014" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "   complement(1808..2209)" 1))) 
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FEATURES             Location/Qualifiers
     misc_marker     4..27
                     /vntifkey="22"
                     /label=FLAG\epitope
     intron          4505..5429
                     /vntifkey="15"
                     /label=intron\AG\promoter
     promoter        4136..5477
                     /vntifkey="29"
                     /label=AG\promoter
     promoter        3753..4130
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     CDS             complement(2753..3613)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      766..857
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    858..1214
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(1292..1374)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1516..1591
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      1622..1696
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1622..1696)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(1811..2212)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             34..753
                     /vntifkey="4"
                     /label=hIFIH1\CARD
BASE COUNT     1199 a      1426 c      1521 g      1343 t 
ORIGIN
        1 atggactaca aggacgacga tgacaagggt accatgtcga atgggtattc cacagacgag 
       61 aatttccgct atctcatctc gtgcttcagg gccagggtga aaatgtacat ccaggtggag 
      121 cctgtgctgg actacctgac ctttctgcct gcagaggtga aggagcagat tcagaggaca 
      181 gtcgccacct ccgggaacat gcaggcagtt gaactgctgc tgagcacctt ggagaaggga 
      241 gtctggcacc ttggttggac tcgggaattc gtggaggccc tccggagaac cggcagccct 
      301 ctggccgccc gctacatgaa ccctgagctc acggacttgc cctctccatc gtttgagaac 
      361 gctcatgatg aatatctcca actgctgaac ctccttcagc ccactctggt ggacaagctt 
      421 ctagttagag acgtcttgga taagtgcatg gaggaggaac tgttgacaat tgaagacaga 
      481 aaccggattg ctgctgcaga aaacaatgga aatgaatcag gtgtaagaga gctactaaaa 
      541 aggattgtgc agaaagaaaa ctggttctct gcatttctga atgttcttcg tcaaacagga 
      601 aacaatgaac ttgtccaaga gttaacaggc tctgattgct cagaaagcaa tgcagagatt 
      661 gagaatttat cacaagttga tggtcctcaa gtggaagagc aacttctttc aaccacagtt 
      721 cagccaaatc tggagaagga ggtctggggc atgtgactcg acgaagatct ttttccctct 
      781 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg 
      841 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca 
      901 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca 
      961 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg 
     1021 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt 
     1081 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat 
     1141 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt 
     1201 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt 
     1261 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 
     1321 gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 
     1381 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa 
     1441 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 
     1501 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 
     1561 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 
     1621 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     1681 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     1741 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg 
     1801 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     1861 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcagggata 
     1921 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     1981 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     2041 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     2101 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     2161 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     2221 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     2281 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     2341 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     2401 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 
     2461 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     2521 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     2581 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     2641 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
     2701 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
     2761 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
     2821 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
     2881 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
     2941 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
     3001 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
     3061 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 
     3121 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 
     3181 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 
     3241 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 
     3301 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
     3361 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 
     3421 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 
     3481 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
     3541 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 
     3601 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
     3661 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
     3721 catttccccg aaaagtgcca cctgggtcga tcgacattga ttattgacta gttattaata 
     3781 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 
     3841 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 
     3901 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 
     3961 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 
     4021 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 
     4081 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt 
     4141 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta 
     4201 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc 
     4261 cccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc 
     4321 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg 
     4381 gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt 
     4441 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc 
     4501 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg 
     4561 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt 
     4621 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg 
     4681 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct 
     4741 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg 
     4801 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg 
     4861 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc 
     4921 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg 
     4981 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg 
     5041 ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc 
     5101 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg 
     5161 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt 
     5221 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc 
     5281 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac 
     5341 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc 
     5401 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt 
     5461 ctcatcattt tggcaaagaa ttcctcgag 
//
