LOCUS       pCAGGS-FLAG-STING        5900 bp    DNA     circular     17-NOV-2011
DEFINITION  LMBP 6921.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6921
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-NOV-2011
            
            
COMMENT     VNTDATE|605185794|
COMMENT     VNTNAME|pCAGGS-FLAG-STING|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-FLAG-STING" 0 5900 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglI" "BglII" "EcoRI" "SalI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5900 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 7 86 0 438 0 1966 0 
COMMENT            3358 0 4270 0 4392 0 4463 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglII" "agatct" 2 0 2 456 0 1177 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 5890 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 1169 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 1 3837 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "FLAG epitope" 22 0 0 4 27 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4..27") 
COMMENT        #6=(CFSignal (CObList) "intron AG promoter" 15 0 0 4916 5840 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4916..5840") 
COMMENT        #7=(CFSignal (CObList) "AG promoter" 29 0 0 4547 5888 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4547..5888") 
COMMENT        #8=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4164 4541 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4164..4541") 
COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3164 4024 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3164..4024)") 
COMMENT        #10=(CFSignal (CObList) "3' UTR of rabbit  \337-globin, incl. polyA" 43 
COMMENT             0 0 1177 1268 0 (CStringList) (CStringList) 1 1 1 1 "1177..1268") 
COMMENT        #11=(CFSignal (CObList) "3' flanking region of rabbit  \337-globin" 21 0 
COMMENT             0 1269 1625 0 (CStringList) (CStringList) 1 1 1 1 "1269..1625") 
COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 1703 1785 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1703..1785)") 
COMMENT        #13=(CFSignal (CObList) "SV40 ori" 33 0 0 1927 2002 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1927..2002") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2033 2107 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2033..2107") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2033 2107 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2033..2107)") 
COMMENT        #16=(CFSignal (CObList) "pMB1 ori" 33 0 1 2222 2623 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2222..2623)") 
COMMENT        #17=(CFSignal (CObList) "hTMEM173" 4 0 0 28 1167 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "28..1167")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #18=(CFSignal (CObList) "LMBP 6921" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 17-NOV-2011" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-FLAG-STING'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 6921." 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: Geert van Loo" 
COMMENT                          1) 
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COMMENT                          "Created: 10/28/11 11:09" 1) 
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COMMENT                          "Last Modified: 11/17/11 11:31" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5900 bp" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 28-OCT-2011" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 6921") 
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COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
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COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                   #66=(CLinePar 
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COMMENT                                        "   4..27" 1))) "FLAG epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4164..4541" 1))) "hCMV-IE enhancer") 
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COMMENT                                        "Start: 4547  End: 5888" 1) 
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COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4547..5888" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "   complement(1703..1785)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #85=(CLinePar 
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COMMENT                                        "   1927..2002" 1))) "SV40 ori") 
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COMMENT                                        "   complement(2222..2623)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                                 "3' UTR of rabbit  \337-globin, incl. polyA") 
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COMMENT                   "Feature Map") 
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COMMENT                            7 4 "GCCNNNNNGGC" "CGGNNNNNCCG"))) 
COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     misc_marker     4..27
                     /vntifkey="22"
                     /label=FLAG\epitope
     intron          4916..5840
                     /vntifkey="15"
                     /label=intron\AG\promoter
     promoter        4547..5888
                     /vntifkey="29"
                     /label=AG\promoter
     promoter        4164..4541
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     CDS             complement(3164..4024)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      1177..1268
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\\ß-globin,\incl.\polyA
     misc_feature    1269..1625
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\\ß-globin
     promoter        complement(1703..1785)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1927..2002
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      2033..2107
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2033..2107)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(2222..2623)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             28..1167
                     /vntifkey="4"
                     /label=hTMEM173
BASE COUNT     1222 a      1619 c      1640 g      1419 t 
ORIGIN
        1 atggactaca aggacgacga tgacaagatg ccccactcca gcctgcatcc atccatcccg 
       61 tgtcccaggg gtcacggggc ccagaaggca gccttggttc tgctgagtgc ctgcctggtg 
      121 accctttggg ggctaggaga gccaccagag cacactctcc ggtacctggt gctccaccta 
      181 gcctccctgc agctgggact gctgttaaac ggggtctgca gcctggctga ggagctgcac 
      241 cacatccact ccaggtaccg gggcagctac tggaggactg tgcgggcctg cctgggctgc 
      301 cccctccgcc gtggggccct gttgctgctg tccatctatt tctactactc cctcccaaat 
      361 gcggtcggcc cgcccttcac ttggatgctt gccctcctgg gcctctcgca ggcactgaac 
      421 atcctcctgg gcctcaaggg cctggcccca gctgagatct ctgcagtgtg tgaaaaaggg 
      481 aatttcaacg tggcccatgg gctggcatgg tcatattaca tcggatatct gcggctgatc 
      541 ctgccagagc tccaggcccg gattcgaact tacaatcagc attacaacaa cctgctacgg 
      601 ggtgcagtga gccagcggct gtatattctc ctcccattgg actgtggggt gcctgataac 
      661 ctgagtatgg ctgaccccaa cattcgcttc ctggataaac tgccccagca gaccgctgac 
      721 cgtgctggca tcaaggatcg ggtttacagc aacagcatct atgagcttct ggagaacggg 
      781 cagcgggcgg gcacctgtgt cctggagtac gccaccccct tgcagacttt gtttgccatg 
      841 tcacaataca gtcaagctgg ctttagccgg gaggataggc ttgagcaggc caaactcttc 
      901 tgccagacac ttgaggacat cctggcagat gcccctgagt ctcagaacaa ctgccgcctc 
      961 attgcctacc aggaacctgc agatgacagc agcttctcgc tgtcccagga ggttctccgg 
     1021 cacctgcggc aggaggaaaa ggaagaggtt actgtgggca gcttgaagac ctcagcggtg 
     1081 cccagtacct ccacgatgtc ccaagagcct gagctcctca tcagtggaat ggaaaagccc 
     1141 ctccctctcc gcacggattt ctcttgagtc gacgaagatc tttttccctc tgccaaaaat 
     1201 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt 
     1261 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg 
     1321 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat 
     1381 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc 
     1441 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata 
     1501 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta 
     1561 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg 
     1621 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 
     1681 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 
     1741 ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 
     1801 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc 
     1861 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
     1921 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
     1981 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt 
     2041 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     2101 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     2161 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     2221 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     2281 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggat aacgcaggaa 
     2341 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     2401 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     2461 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     2521 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     2581 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     2641 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     2701 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     2761 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     2821 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 
     2881 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     2941 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     3001 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     3061 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     3121 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     3181 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     3241 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     3301 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     3361 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     3421 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     3481 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     3541 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     3601 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     3661 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     3721 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     3781 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     3841 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     3901 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     3961 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     4021 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     4081 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     4141 gaaaagtgcc acctgggtcg atcgacattg attattgact agttattaat agtaatcaat 
     4201 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
     4261 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
     4321 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
     4381 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
     4441 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
     4501 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 
     4561 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 
     4621 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg 
     4681 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca 
     4741 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 
     4801 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc 
     4861 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 
     4921 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 
     4981 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 
     5041 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 
     5101 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 
     5161 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 
     5221 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 
     5281 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 
     5341 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 
     5401 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc 
     5461 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt 
     5521 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga 
     5581 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg 
     5641 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc 
     5701 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg 
     5761 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg 
     5821 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt 
     5881 ttggcaaaga attcctcgag 
//
