LOCUS       pCAGGS-E-hsi-I-PA0072        6067 bp    DNA     circular     11-MAR-2011
DEFINITION  LMBP 6925.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6925
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-MAR-2011
            
COMMENT     VNTDATE|584368400|
COMMENT     VNTNAME|pCAGGS-E-hsi-I-PA0072|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hsi-I-PA0072" 0 6067 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "NotI" "PvuI" "SacI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6067 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 2007 0 2344 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 4 0 1277 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3788 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1265 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "intron AG promoter" 15 0 0 5092 6016 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6303..7227") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3344 4204 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4555..5415)") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 2402 2803 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3613..4014)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2213 2287 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3424..3498)") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2213 2287 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3424..3498") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 2107 2182 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3318..3393") 
COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 1883 1965 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3094..3176)") 
COMMENT        #13=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 1357 1448 0 (CStringList) (CStringList) 1 1 1 1 "2568..2659") 
COMMENT        #14=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #15=(CFSignal (CObList) "AG promoter" 29 0 0 4723 6064 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5934..7275") 
COMMENT        #16=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4340 4717 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #17=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 1449 1805 0 (CStringList) (CStringList) 1 1 1 1 "2660..3016") 
COMMENT        #18=(CFSignal (CObList) "PAO0072" 4 0 0 64 1263 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #19=(CFSignal (CObList) "Last modified: 11-MAR-2011" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "LMBP 6925" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                    2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-hsi-I-PA0072'" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 6925." 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/11/11 12:33" 1) 
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COMMENT                          "Last Modified: 03/11/11 12:33" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6067 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 25-SEP-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
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COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                 "3' flanking region of rabbit \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                                   #71=(CLinePar 
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COMMENT                                        "   5551..5928" 1))) "hCMV-IE enhancer") 
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COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #75=(CLinePar 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                        "   5934..7275" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #81=(CLinePar 
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COMMENT                                        "   complement(3094..3176)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                   #86=(CLinePar 
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COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     intron          5092..6016
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3344..4204)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2402..2803)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2213..2287)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2213..2287
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      2107..2182
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1883..1965)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1357..1448
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        4723..6064
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4340..4717
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1449..1805
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..1263
                     /vntifkey="4"
                     /label=PAO0072
BASE COUNT     1153 a      1759 c      1779 g      1376 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcaatgcgcg ccggcctgct gctctcgctg gcctgggccg actaccgcgg cgaggcgcgc 
      121 ctgtcgctct gcgcgatctt cgccctggcg gcggtgatca ccccgctgct ggtgctgttc 
      181 ggcctcaagt acggcctggt cagcaccctc accgagcggc tggagcgcgc gccttcggtg 
      241 cgcgagatca ttccggtcgg cggcgcccgc taccgtgccg aggacatcgc cgccctggcc 
      301 gcacgcgcgg acgtcgcctt cgccgtgccg cgcacccggc agatcgccgc caccgccgac 
      361 ctcagcggcg gcggcgagac cgccctgagc gtcgagatga ttcccagcgc cgccggcgac 
      421 ccgctgctgg ccggcctgcc gcaacccgac cggccgaccc gcgtggtgct cagccacggc 
      481 gccgcggaaa aactcggcgc ccagcctggc gagcgcatcg tcgcgcgcat cggccggcgc 
      541 atggatggcc aggcacagag ccagcgcctg gagctggaag tgctcgcggt gctgccgcag 
      601 gagcgtttcg cccgcgacgc gctgttcgcc ccgctggccc tgctggaagc cgccgaggac 
      661 tatcgcgacg gtcgcgcggt ggccgcctac ggctggccgg gaaaggccgg cgagaccccg 
      721 cgcgcacgca tctacccggg cttccgcctg tacgcccgag acctcgatgc ggtggaaggg 
      781 ctgcgccggc acttcgtcgc gagtggcgtg gaagtcgcca cccaggccga ggccatcgcc 
      841 caggtgcgca gcctcagccg caacctcggc ctggtgttct ggatcgtcgc cgcgctggcc 
      901 atcggcggtg ccttcgccgc catcgccgcc agcagcctgg ccgccgtcga acgcaagcgc 
      961 cgggccctgg cggtgctacg tctgctgggc ttcccgaccg ccgcgctggt cggcttcgtc 
     1021 atgctcctgg cgctgttcag cgccgtcttc ggcctgttcc tcgccgggct gctgtacgcc 
     1081 gccacggccg gcgcgctgaa ccacctgttc gacaaccaga gcggcgagtt cgtctgccgc 
     1141 ctgctgccca gccactacct gacggcgctg ctcgccacgc tgctgtgcag cgtcctggcc 
     1201 gccgccagcg gcggctggcg cgcggcgcgg atcgaagccg cagagggatt acgcgatgtt 
     1261 tgactcgagg tcgaggaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct 
     1321 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat 
     1381 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt 
     1441 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg 
     1501 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat 
     1561 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc 
     1621 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata 
     1681 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta 
     1741 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg 
     1801 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 
     1861 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 
     1921 ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 
     1981 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc 
     2041 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
     2101 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
     2161 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt 
     2221 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     2281 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     2341 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     2401 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     2461 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggat aacgcaggaa 
     2521 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     2581 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     2641 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     2701 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     2761 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     2821 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     2881 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     2941 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     3001 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 
     3061 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     3121 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     3181 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     3241 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     3301 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     3361 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     3421 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     3481 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     3541 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     3601 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     3661 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     3721 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     3781 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     3841 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     3901 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     3961 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     4021 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     4081 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     4141 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     4201 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     4261 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     4321 gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg 
     4381 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc 
     4441 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc 
     4501 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact 
     4561 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat 
     4621 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact 
     4681 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc 
     4741 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt 
     4801 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc aggcggggcg 
     4861 gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc caatcagagc 
     4921 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag 
     4981 cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc 
     5041 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg 
     5101 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct 
     5161 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc 
     5221 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg 
     5281 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc 
     5341 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa 
     5401 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg 
     5461 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg 
     5521 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg 
     5581 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga aggggcgcgg 
     5641 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg 
     5701 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc 
     5761 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag 
     5821 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct 
     5881 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg 
     5941 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt 
     6001 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg 
     6061 caaagaa 
//
