LOCUS       pCAGGS-E-plcB           5854 bp    DNA     circular     11-MAR-2011
DEFINITION  LMBP 6927.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6927
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-MAR-2011

COMMENT     VNTDATE|584368558|
COMMENT     VNTNAME|pCAGGS-E-plcB|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-plcB" 0 5854 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "NotI" "PvuI" "SacI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5854 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1794 0 2131 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 4 0 1064 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 636 0 3575 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1052 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "intron AG promoter" 15 0 0 4879 5803 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6303..7227") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3131 3991 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4555..5415)") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 2189 2590 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3613..4014)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2000 2074 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3424..3498)") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2000 2074 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3424..3498") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 1894 1969 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3318..3393") 
COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 1670 1752 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3094..3176)") 
COMMENT        #13=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 1144 1235 0 (CStringList) (CStringList) 1 1 1 1 "2568..2659") 
COMMENT        #14=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #15=(CFSignal (CObList) "AG promoter" 29 0 0 4510 5851 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5934..7275") 
COMMENT        #16=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4127 4504 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #17=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 1236 1592 0 (CStringList) (CStringList) 1 1 1 1 "2660..3016") 
COMMENT        #18=(CFSignal (CObList) "PlcB" 4 0 0 64 1050 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #19=(CFSignal (CObList) "Last modified: 11-MAR-2011" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "LMBP 6927" 2 0 0 0 0 0 (CStringList) 
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COMMENT                   5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-plcB'" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 6927." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/11/11 12:35" 1) 
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COMMENT                          "Last Modified: 03/11/11 12:35" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5854 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 25-SEP-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
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COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                 "3' flanking region of rabbit \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5551..5928" 1))) "hCMV-IE enhancer") 
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COMMENT                                        "Start: 4510  End: 5851" 1) 
COMMENT                                   #74=(CLinePar 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #75=(CLinePar 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                        "   5934..7275" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #81=(CLinePar 
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COMMENT                                        "   complement(3094..3176)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "Start: 1894  End: 1969" 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
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COMMENT                                        "   3318..3393" 1))) "SV40 ori") 
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COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     intron          4879..5803
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3131..3991)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2189..2590)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2000..2074)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2000..2074
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      1894..1969
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1670..1752)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1144..1235
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        4510..5851
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4127..4504
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1236..1592
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..1050
                     /vntifkey="4"
                     /label=PlcB
BASE COUNT     1198 a      1665 c      1650 g      1341 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcaatgaaaa ccttcgcccg cctggccgcc ggtctctgca tctgcgccat cgccagccag 
      121 gccaacgcct ggtcgcaacc gacccacaag aacatcgtca aggatgccct ggccttcatg 
      181 aactcctcct acgccaccga ggagatgcgc caggcctacc ggttctacgt gagcgccgcc 
      241 ggcagcgagg cgcaggccgg ggagatcctc ggccaggcgg ccttcgacgt ggacgacttc 
      301 aaggacaccc gtctcggtgg ctggtgggtc ggctacgaac acgcgccgct gtggggcgcg 
      361 gcttcgggca tcgtcaacta cacctcgtac tggcacttcc tcaacctggc ccgggacggc 
      421 gactcccatg gcaaccccca cggcggctac gactaccgct accacaaggt cgacggcggc 
      481 atcgccgacg tcgactggta cgccatggtc tacctctaca accgcgagct gaagcgcgag 
      541 gacttcgaca ccaccgaggc gcactaccgc cagggcaccc gctcggactg gcaggagcac 
      601 tacggcgact tccagaccgc cgccttccag ccgatcgata acctcgccac ctactggttc 
      661 gagcagttcc gcgccgcgcc ttcgctgcag accatcggct atgccctgca tgccaccggc 
      721 gacgtggccc agccgcacca tgtgtggatc acctcggcca acggccattc cagctgggaa 
      781 ggctgggtcg acgaccacta cgccagcgag aagctcaacg acccggcggc ggtggccaac 
      841 ctggtggggc gctacgaccc gagcaagagc atccgcgacc tgctcaccca gaccggccag 
      901 gtcgcctacg cgcgccccga gccgctctac gacaccagct acgagacgcg cctgcgggtg 
      961 gcgaaggagc tgattcccga gtccatcgcc ctgaccgtca ccgtactgac caagggcgcc 
     1021 aacagcttcg atgcaccgac tgctctctga ctcgaggtcg aggaattcac tcctcaggtg 
     1081 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac 
     1141 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg 
     1201 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt 
     1261 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg 
     1321 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa 
     1381 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc 
     1441 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc 
     1501 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca 
     1561 gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta 
     1621 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 
     1681 acgagccgga agcataaagt gtaaagcctg ggtgcctaat gagtgagcta actcacatta 
     1741 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc 
     1801 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     1861 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     1921 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     1981 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa 
     2041 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 
     2101 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc 
     2161 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 
     2221 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     2281 tccacagaat cagggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     2341 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     2401 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     2461 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     2521 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     2581 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     2641 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     2701 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     2761 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     2821 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     2881 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     2941 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     3001 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     3061 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     3121 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     3181 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     3241 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     3301 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     3361 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     3421 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     3481 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     3541 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     3601 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     3661 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     3721 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     3781 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     3841 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     3901 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     3961 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     4021 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     4081 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca ttgattattg 
     4141 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc 
     4201 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 
     4261 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 
     4321 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 
     4381 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 
     4441 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 
     4501 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca 
     4561 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg 
     4621 gggggggggc cccccccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg 
     4681 aaaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg 
     4741 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgttgcgcgc 
     4801 tgccttcccc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg 
     4861 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag 
     4921 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc 
     4981 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg 
     5041 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg 
     5101 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg 
     5161 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga 
     5221 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt 
     5281 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc 
     5341 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc 
     5401 ggggagggct cgggggaagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc 
     5461 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt 
     5521 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg 
     5581 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc 
     5641 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg 
     5701 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag 
     5761 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct 
     5821 ggttattgtg ctgtctcatc attttggcaa agaa 
//
