LOCUS       pCAGGS-E-hsiE           5713 bp    DNA     circular     11-MAR-2011
DEFINITION  LMBP 6928.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6928
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-MAR-2011

COMMENT     VNTDATE|584367816|
COMMENT     VNTNAME|pCAGGS-E-hsiE|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hsiE" 0 5713 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "NotI" "PvuI" "SacI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5713 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1653 0 1990 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 4 0 923 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3434 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 587 0 911 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "intron AG promoter" 15 0 0 4738 5662 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6303..7227") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2990 3850 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4555..5415)") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 2048 2449 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3613..4014)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1859 1933 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3424..3498)") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1859 1933 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3424..3498") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 1753 1828 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3318..3393") 
COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 1529 1611 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3094..3176)") 
COMMENT        #13=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 1003 1094 0 (CStringList) (CStringList) 1 1 1 1 "2568..2659") 
COMMENT        #14=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #15=(CFSignal (CObList) "AG promoter" 29 0 0 4369 5710 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5934..7275") 
COMMENT        #16=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3986 4363 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #17=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 1095 1451 0 (CStringList) (CStringList) 1 1 1 1 "2660..3016") 
COMMENT        #18=(CFSignal (CObList) "HsiE" 4 0 0 64 909 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
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COMMENT        #19=(CFSignal (CObList) "Last modified: 11-MAR-2011" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "LMBP 6928" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-hsiE'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/11/11 12:23" 1) 
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COMMENT                          "Last Modified: 03/11/11 12:23" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5713 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Modification Date in the Original DB: 25-SEP-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                 "3' flanking region of rabbit \337-globin"))) 
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COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                                   #71=(CLinePar 
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COMMENT                                   #74=(CLinePar 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
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COMMENT                                   #76=(CLinePar 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     intron          4738..5662
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(2990..3850)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2048..2449)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(1859..1933)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      1859..1933
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      1753..1828
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1529..1611)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1003..1094
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        4369..5710
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3986..4363
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1095..1451
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..909
                     /vntifkey="4"
                     /label=HsiE
BASE COUNT     1140 a      1586 c      1662 g      1325 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcaatggccg acccttcatt cgcatccggg cgcctgggct cccggctaca gggaagtatc 
      121 gcgatgatcg ccgaagaact gttgcgcgcc gggcgcctgg acgatgcctt gaaagccttg 
      181 caggaacagg tgcgcagcca gccgtcgaac gctaccctgc ggatcttcct gttccagctg 
      241 ctggccgtga tgggccagtg ggcgcgggcg cagaaccagc tcaaggtggt cggcgaactg 
      301 gatgcctcgg cgctgccgat ggtacagacc tactccaccg ccatcgactg cgaggcgctg 
      361 cgccgcgagg tgttcgccgg gcgcctgacg ccggtgatcc tcggccagcc ggcggaatgg 
      421 atcgcgccgc tgttgcaggc cctgagcctg gacgccgaag gccatggcga ggccgcccag 
      481 gccctgcgcg agcaggcctt cgacgccgcg ccggcggtgc ccggtcggat cggcgaagcg 
      541 ccgttcgcct ggctggcgga tgccgacacg cgcctcggcc cggtgctcga ggtaatcgtc 
      601 aacggtcgct atgcctggtt gccgatgagc aacctgcgca gcctcaaggt cgaggcgccg 
      661 agcgacctgc gcgatctggt ctggctgccg gccgaactga ccctggccaa cggtggcgcc 
      721 accgtcgcgc tgctgccggc gcgctacgcg gaaaccgtcg agcacggcga cgacgccgcg 
      781 cgcctgggac gcaagaccga atggctggac agcggcctgc cggtcggcca gcggctgttc 
      841 gtcaccgatg ccggcgagac cgcgctgttc gacctgcgtg aactggactt cgagccgacg 
      901 gacgcctgac tcgaggtcga ggaattcact cctcaggtgc aggctgccta tcagaaggtg 
      961 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc 
     1021 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa 
     1081 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat 
     1141 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat 
     1201 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa 
     1261 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt 
     1321 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt 
     1381 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc 
     1441 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc 
     1501 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     1561 taaagcctgg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 
     1621 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca 
     1681 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     1741 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 
     1801 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa 
     1861 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     1921 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
     1981 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     2041 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     2101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg 
     2161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     2221 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     2281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     2341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     2401 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     2461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     2521 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     2581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     2641 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 
     2701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     2761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     2821 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     2881 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
     2941 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
     3001 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     3061 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     3121 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     3181 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     3241 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     3301 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     3361 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     3421 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
     3481 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     3541 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     3601 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     3661 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     3721 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     3781 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     3841 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
     3901 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
     3961 ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca 
     4021 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 
     4081 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 
     4141 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 
     4201 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 
     4261 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 
     4321 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc 
     4381 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt 
     4441 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc 
     4501 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat 
     4561 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat 
     4621 aaaaagcgaa gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc 
     4681 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 
     4741 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 
     4801 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 
     4861 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc 
     4921 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt 
     4981 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg 
     5041 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg 
     5101 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg 
     5161 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg 
     5221 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg 
     5281 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct 
     5341 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc 
     5401 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc 
     5461 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct 
     5521 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag 
     5581 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca 
     5641 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca 
     5701 ttttggcaaa gaa 
//
