LOCUS       pBR322cI857             5278 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 693.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBR322cI857|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBR322cI857" 0 5278 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5278 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "PRM" 30 0 1 1136 1184 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1136..1184)") 
COMMENT        #4=(CFSignal (CObList) "PR" 30 0 0 1151 1217 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1151..1217") 
COMMENT        #5=(CFSignal (CObList) "ORI" 33 0 1 3270 3670 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3270..3670)") 
COMMENT        #6=(CFSignal (CObList) "AMP" 4 0 1 4210 5070 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4210..5070)") 
COMMENT        #7=(CFSignal (CObList) "lambda cI857" 4 0 1 422 1135 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
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COMMENT                         "DNA 'pBR322cI857'" 1) 
COMMENT                    #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                         1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 5278 bp" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1) 
COMMENT                    #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Modification Date in the Original DB: 19-MAR-1980" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                  "Annotations") 
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COMMENT                                 (CObjectList 
COMMENT                                  #26=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 422   End: 1135 (Complementary)" 
COMMENT                                       1))) "lambda cI857") 
COMMENT                           #27=(CFolderPar 
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COMMENT                                 (CParagraph 4210 (3 #6# 0) 1 2 2 0 327) 
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COMMENT                                  #28=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 4210  End: 5070 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #29=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
COMMENT                                  #30=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #31=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(4210..5070)" 1))) "AMP"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                       1) 
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COMMENT                                       "phage \361 promoter for repressor maintenance" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #37=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(1136..1184)" 1))) "PRM") 
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COMMENT                                  #40=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "phage \361 major rightward promoter" 1) 
COMMENT                                  #41=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #42=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                         "Promoter Prokaryotic (2 total)") 
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COMMENT                                       "Start: 3270  End: 3670 (Complementary)" 
COMMENT                                       1) 
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COMMENT                                       "origin plasmid" 1) 
COMMENT                                  #47=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #48=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(3270..3670)" 1))) "ORI"))) 
COMMENT                         "Replication Origin (1 total)"))) "Feature Map")))) 
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FEATURES             Location/Qualifiers
     promoter        complement(1136..1184)
                     /vntifkey="30"
                     /label=PRM
                     /note="phage ñ promoter for repressor maintenance"
     promoter        1151..1217
                     /vntifkey="30"
                     /label=PR
                     /note="phage ñ major rightward promoter"
     rep_origin      complement(3270..3670)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4210..5070)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     CDS             complement(422..1135)
                     /vntifkey="4"
                     /label=lambda\cI857
BASE COUNT     1213 a      1444 c      1336 g      1285 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatcc cccccccccc cccccccccg ccgaccagaa caccttgccg 
      421 atcagccaaa cgtctcttca ggccactgac tagcgataac tttccccaca acggaacaac 
      481 tctcattgca tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc 
      541 tatccctgat cagtttcttg aaggtaaact catcaccccc aagtctggct atgcagaaat 
      601 cacctggctc aacagcctgc tcagggtcaa cgagaattaa cattccgtca ggaaagcttg 
      661 gcttggagcc tgttggtgcg gtcatggaat taccttcaac ctcaagccag aatgcagaat 
      721 cactggcttt tttggttgtg cttacccatc tctccgcatc acctttggta aaggttctaa 
      781 gctcaggtga gaacatccct gcctgaacat gagaaaaaac agggtactca tactcacttc 
      841 taagtgacgg ctgcatacta accgcttcat acatctcgta gatttctctg gcgattgaag 
      901 ggctaaattc ttcaacgcta actttgagaa tttttgcaag caatgcggcg ttataagcat 
      961 ttaatgcatt gatgccatta aataaagcac caacgcctga ctgccccatc cccatcttgt 
     1021 ctgcgacaga ttcctgggat aagccaagtt catttttctt tttttcataa attgctttaa 
     1081 ggcgacgtgc gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg ctcatacgtt 
     1141 aaatctatca ccgcaaggga taaatatcta acaccgtgcg tgttgactat tttacctctg 
     1201 gcggtgataa tggttgcatg tactaaggag gttgtatgga acaacgcata accctgaaag 
     1261 attatgcaat gcgggggggg gggggggggg gggatcctct acgccggacg catcgtggcc 
     1321 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg 
     1381 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca 
     1441 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg 
     1501 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag 
     1561 ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg 
     1621 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga 
     1681 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg 
     1741 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc 
     1801 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc 
     1861 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt 
     1921 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg 
     1981 caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta 
     2041 acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg 
     2101 aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt 
     2161 cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg 
     2221 gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa 
     2281 ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca 
     2341 tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga 
     2401 cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc 
     2461 gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct 
     2521 tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt 
     2581 tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa 
     2641 cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca 
     2701 gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg 
     2761 tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca 
     2821 cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa 
     2881 gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca 
     2941 tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg 
     3001 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 
     3061 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 
     3121 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc 
     3181 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 
     3241 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 
     3301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     3361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     3421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     3481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     3541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     3601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     3661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     3721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     3781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     3841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
     3901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     3961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     4021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     4081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     4141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     4201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     4261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     4321 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     4381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     4441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     4501 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat 
     4561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 
     4621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 
     4681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 
     4741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 
     4801 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt 
     4861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 
     4921 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 
     4981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 
     5041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 
     5101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 
     5161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 
     5221 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa 
//
